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The map label for this gene is isp [H]

Identifier: 77461918

GI number: 77461918

Start: 6385775

End: 6386956

Strand: Direct

Name: isp [H]

Synonym: Pfl01_5698

Alternate gene names: 77461918

Gene position: 6385775-6386956 (Clockwise)

Preceding gene: 77461917

Following gene: 77461921

Centisome position: 99.18

GC content: 53.98

Gene sequence:

>1182_bases
TTGTCATTGCAAGGATCGCATATGGATAAGTACATCGTTCTACGAAAAACCTTCGGTCACACCAGCTCGATAAAGCGTAC
TGCGTCCGCCGCTGTGTCGGACATCACGCTGGGTGTTGAGCACATCCCGCTGCATGCGTTGCCCACTATAAGTAATGATC
CAGAAGTAGTGGCTTTAACTCCGCCGATGGCCACAAAACTAATCCAGCCCATGAAAAGCTCGACTGTCCAGCAGTCAAAG
GGAGACTCTTGGGGAATAGCTGCCGTCGGCGCCGATCGATCGCCCTTTACCGGAGCCGACGTGACTGTCGCTGTACTGGA
TACGGGCATAGACAAATCTCACCCTGCCTTTGCTGGTATGGATATTGTGGAGCACGACTTCACTGGCGAAGGCAACGGTG
ACGTGAACGGCCACGGGACTCACTGCGCAGGAACAATCTTTGGTCGTGACGTAAACGGGCAGAGGATAGGCATAGCCCGG
GGCGTCAGCCGCGCTGTCATCGGCAAAGTGATTGGACAGAACGATGGCGACTCCCAAATGATATTCGAAGGGCTTCAGTG
GGCACTTCAACAACGAGCAAACATCATCTCAATGTCACTCGGCTTTGATTTTCCTGGCGAAGTCCAGAGCAAGGTCAATG
ATGGATGGCCCGTGGATCTGGCCACTTCCCAAGCGCTGGAAACATACCGTGGAAACTTAAGGTTGCTCGATGCGCTGATG
GGCCTGTTCAAAGCTCAATCTGCTTTCAACTTAGCACCTTTGGTGATTGCTGCAGCAGGTAATGAAAGCCGGCGCATGAT
CAATAGCCAGTATAAAATTGCAGCGTCCCTACCGGCTTCTGCGCAGGACGTGATTTCTGTGGCAGCTGTTTCGCCCGCAA
TGCAAATACAGTACGAAGTAGCAGATTTCTCCAATAGTTTCCCCCAGTTATCCGCCCCCGGCGTAGACATAGTATCCGCT
TGGCCGGGTGGAGCTCTGCGCTCTTCTGACGGTACAAGTATGGCTTGCCCTCACGTTGCGGGGGTCGCTGCTCTATGGTG
GCAGCGCGCAGTTGAAAGCAAATCTACGAACCGGGCTTGGGCCGTCGCCGCAAACCTCACCACGCACGCTCGACCCAACT
GCTTCACAAGTTCATTTGACGCAAGCGACTTTGGCCACGGACTAGTCACAGCGCCTTTGTAA

Upstream 100 bases:

>100_bases
CTATACGGACATTCTGAAGGCGTATGCCGATACCTTGGTGCCGATTGAACCACAGGTTTCGCTGCCTATCCGGGTATAAC
AGCAATTCAAAATCCCAGGC

Downstream 100 bases:

>100_bases
TCACGCTCGCGCTGCCGGGGCTCAGCCTCTTTTCCCAATCAATTACTAAGGAAAAATCGTGGAAGAGACGTCTGAAACAT
CCGCTGAACGCTCGCTGCGA

Product: peptidase S8/S53 subtilisin kexin sedolisin

Products: NA

Alternate protein names: ISP-1 [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSLQGSHMDKYIVLRKTFGHTSSIKRTASAAVSDITLGVEHIPLHALPTISNDPEVVALTPPMATKLIQPMKSSTVQQSK
GDSWGIAAVGADRSPFTGADVTVAVLDTGIDKSHPAFAGMDIVEHDFTGEGNGDVNGHGTHCAGTIFGRDVNGQRIGIAR
GVSRAVIGKVIGQNDGDSQMIFEGLQWALQQRANIISMSLGFDFPGEVQSKVNDGWPVDLATSQALETYRGNLRLLDALM
GLFKAQSAFNLAPLVIAAAGNESRRMINSQYKIAASLPASAQDVISVAAVSPAMQIQYEVADFSNSFPQLSAPGVDIVSA
WPGGALRSSDGTSMACPHVAGVAALWWQRAVESKSTNRAWAVAANLTTHARPNCFTSSFDASDFGHGLVTAPL

Sequences:

>Translated_393_residues
MSLQGSHMDKYIVLRKTFGHTSSIKRTASAAVSDITLGVEHIPLHALPTISNDPEVVALTPPMATKLIQPMKSSTVQQSK
GDSWGIAAVGADRSPFTGADVTVAVLDTGIDKSHPAFAGMDIVEHDFTGEGNGDVNGHGTHCAGTIFGRDVNGQRIGIAR
GVSRAVIGKVIGQNDGDSQMIFEGLQWALQQRANIISMSLGFDFPGEVQSKVNDGWPVDLATSQALETYRGNLRLLDALM
GLFKAQSAFNLAPLVIAAAGNESRRMINSQYKIAASLPASAQDVISVAAVSPAMQIQYEVADFSNSFPQLSAPGVDIVSA
WPGGALRSSDGTSMACPHVAGVAALWWQRAVESKSTNRAWAVAANLTTHARPNCFTSSFDASDFGHGLVTAPL
>Mature_392_residues
SLQGSHMDKYIVLRKTFGHTSSIKRTASAAVSDITLGVEHIPLHALPTISNDPEVVALTPPMATKLIQPMKSSTVQQSKG
DSWGIAAVGADRSPFTGADVTVAVLDTGIDKSHPAFAGMDIVEHDFTGEGNGDVNGHGTHCAGTIFGRDVNGQRIGIARG
VSRAVIGKVIGQNDGDSQMIFEGLQWALQQRANIISMSLGFDFPGEVQSKVNDGWPVDLATSQALETYRGNLRLLDALMG
LFKAQSAFNLAPLVIAAAGNESRRMINSQYKIAASLPASAQDVISVAAVSPAMQIQYEVADFSNSFPQLSAPGVDIVSAW
PGGALRSSDGTSMACPHVAGVAALWWQRAVESKSTNRAWAVAANLTTHARPNCFTSSFDASDFGHGLVTAPL

Specific function: Major intracellular protease produced by Bacillus subtilis [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6319893, Length=266, Percent_Identity=31.5789473684211, Blast_Score=76, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: NA

Molecular weight: Translated: 41397; Mature: 41266

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00136 SUBTILASE_ASP ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQGSHMDKYIVLRKTFGHTSSIKRTASAAVSDITLGVEHIPLHALPTISNDPEVVALT
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHEECHHHCCCEEECCCCCCCCEEEEC
PPMATKLIQPMKSSTVQQSKGDSWGIAAVGADRSPFTGADVTVAVLDTGIDKSHPAFAGM
CHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
DIVEHDFTGEGNGDVNGHGTHCAGTIFGRDVNGQRIGIARGVSRAVIGKVIGQNDGDSQM
HHEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEHHHHHHHHHHHHHHCCCCCCHHH
IFEGLQWALQQRANIISMSLGFDFPGEVQSKVNDGWPVDLATSQALETYRGNLRLLDALM
HHHHHHHHHHHCCCEEEEECCCCCCHHHHHHCCCCCCEEHHHHHHHHHHCCCHHHHHHHH
GLFKAQSAFNLAPLVIAAAGNESRRMINSQYKIAASLPASAQDVISVAAVSPAMQIQYEV
HHHHHHCCCCCCEEEEEECCCHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEEE
ADFSNSFPQLSAPGVDIVSAWPGGALRSSDGTSMACPHVAGVAALWWQRAVESKSTNRAW
HHCCCCCCCCCCCCCEEEECCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEE
AVAANLTTHARPNCFTSSFDASDFGHGLVTAPL
EEEECCCCCCCCCCCCCCCCCCCCCCCEEECCC
>Mature Secondary Structure 
SLQGSHMDKYIVLRKTFGHTSSIKRTASAAVSDITLGVEHIPLHALPTISNDPEVVALT
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHEECHHHCCCEEECCCCCCCCEEEEC
PPMATKLIQPMKSSTVQQSKGDSWGIAAVGADRSPFTGADVTVAVLDTGIDKSHPAFAGM
CHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
DIVEHDFTGEGNGDVNGHGTHCAGTIFGRDVNGQRIGIARGVSRAVIGKVIGQNDGDSQM
HHEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEHHHHHHHHHHHHHHCCCCCCHHH
IFEGLQWALQQRANIISMSLGFDFPGEVQSKVNDGWPVDLATSQALETYRGNLRLLDALM
HHHHHHHHHHHCCCEEEEECCCCCCHHHHHHCCCCCCEEHHHHHHHHHHCCCHHHHHHHH
GLFKAQSAFNLAPLVIAAAGNESRRMINSQYKIAASLPASAQDVISVAAVSPAMQIQYEV
HHHHHHCCCCCCEEEEEECCCHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEEE
ADFSNSFPQLSAPGVDIVSAWPGGALRSSDGTSMACPHVAGVAALWWQRAVESKSTNRAW
HHCCCCCCCCCCCCCEEEECCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEE
AVAANLTTHARPNCFTSSFDASDFGHGLVTAPL
EEEECCCCCCCCCCCCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3087947; 9384377 [H]