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The map label for this gene is cls [H]

Identifier: 77461885

GI number: 77461885

Start: 6345049

End: 6346488

Strand: Direct

Name: cls [H]

Synonym: Pfl01_5665

Alternate gene names: 77461885

Gene position: 6345049-6346488 (Clockwise)

Preceding gene: 77461883

Following gene: 77461891

Centisome position: 98.55

GC content: 61.32

Gene sequence:

>1440_bases
ATGGATTATTTCGGACCGCACATTTTCGGTTATCTGATCGCCCTGATACACACCCTCGGCTCGATCGCCGCGATCCATGC
CGTATTGACCGTCCGCACCGCCCAAGGCTCGATCGCCTGGGCGCTGTCGCTGATCTTCATTCCCTACCTCACCCTGATTC
CTTATCTGGTGTTCGGCCGCAGCACCTTCGATGGCTACATCAAGGCCCGCCGCCAGGCCAACGAACAGATGCGCCAGGCG
ATTTCCGAACTCAACTGGCGGCCGTGGGTGGAAGAAGCCCTGACCGCCCGCGCCTCGAATGCTTACGACTCATTGCGAGC
GATGCCGAAGCTGGGACGCATGCCGTGCCTGGCCAACAATGAAGTGCGATTGCTGATCAACGGTGCCGCCACCTTCGAGG
CGATTTTCCACGCAATCGACCAGGCGCGGGAAGCGGTGCTGATCCAGTTTTTCATCATTCACGACGATCGTCTTGGTCAG
CGCCTACGCGACCTGCTGCTGAAAAAAGCGGCCGAAGGCGTGGTGATTCATTTGCTCTACGACCGCATCGGCAGCCACGC
CCTGCCCCACAGCTACGTGCAAGCGCTGCGCGATGGCGGTGTCGAGGTCAAGGCGTTCGCCACCCGCAGCGGCTGGCTCA
ATCGTTTCCAGGTGAATTTCCGCAACCACCGCAAGATCGTGGTGGTCGACGGGCTGATCGGGTTTGTCGGCGGGCACAAC
GTAGGCGACGAATACATGGGTGAAAAGCCACCGCTGGCACCGTGGCGCGACACTCACGTTCAGGTGCGCGGCCCGGTGGT
GGCGTGCATGCAGGAGTCATTTGCCGAAGACTGGTTCTGGGCCGCGCGGTCATTGCCGCCGCTGATCCTGCCGGACGCGT
ACCCAGAGGACGGCGTGCTTTGCCAATTGCTCGCCAGCGGCCCGGCGGATGCTTACGAAACCTGCTCGCTGTTCTTCGTT
GAGGCCATCCACGCCGCCACCGAACGCGTGTGGATCACCAGCCCCTACTTCATTCCTGACGAAGCCGTGTTCGCGGCGTT
GCGCCTCGCAGTTTTGCGCGGGGTCGACGTGCGCCTGTTGCTGCCTTCGCGTCCCGACCACCGCATCGTCTACGCAGCCT
CCAGCCTGTACGCCTTCGAAGCGGTACGCGCCGGCGTGCGGGTATTCCGCTATGAACCGGGTTTTCTGCATCAGAAGGTG
GTGTTGATCGACAGTGAAATCAGCGCCATCGGCAGCGCCAATCTGGACAATCGTTCGTTCCGGCTTAATTTCGAGGTGAT
GCTGCTGACCGTCGACAGCGAATTCGCCGCCAGCGTGGAACAGATGCTCAACGACGACTTCGCCCAGGCCTACGAGGTCG
CCAAAGAAGAAAGCCGGGAGATCCACCGCCTGCAACAGGTCGGCATGCGGATCGCCCGGCTGATTTCACCGATTCTATAG

Upstream 100 bases:

>100_bases
TTCGGTTCATGACCGGCGCTGGCAGCTTGAAAACAAATAGCGACAATCGCCATGGAACATGGTCTGTTAAGCATTGAATC
TTCAGGAGCAAGGTTGCCCC

Downstream 100 bases:

>100_bases
GTTCAAGGGTTGTAGATGTCGTCCCGGGTCCATGGCAGTTCATGGCTGCCGTCGGCATGGGCCTTCACGGCGAGGATCTG
GTGCAGATTGATCCAGCCGC

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MDYFGPHIFGYLIALIHTLGSIAAIHAVLTVRTAQGSIAWALSLIFIPYLTLIPYLVFGRSTFDGYIKARRQANEQMRQA
ISELNWRPWVEEALTARASNAYDSLRAMPKLGRMPCLANNEVRLLINGAATFEAIFHAIDQAREAVLIQFFIIHDDRLGQ
RLRDLLLKKAAEGVVIHLLYDRIGSHALPHSYVQALRDGGVEVKAFATRSGWLNRFQVNFRNHRKIVVVDGLIGFVGGHN
VGDEYMGEKPPLAPWRDTHVQVRGPVVACMQESFAEDWFWAARSLPPLILPDAYPEDGVLCQLLASGPADAYETCSLFFV
EAIHAATERVWITSPYFIPDEAVFAALRLAVLRGVDVRLLLPSRPDHRIVYAASSLYAFEAVRAGVRVFRYEPGFLHQKV
VLIDSEISAIGSANLDNRSFRLNFEVMLLTVDSEFAASVEQMLNDDFAQAYEVAKEESREIHRLQQVGMRIARLISPIL

Sequences:

>Translated_479_residues
MDYFGPHIFGYLIALIHTLGSIAAIHAVLTVRTAQGSIAWALSLIFIPYLTLIPYLVFGRSTFDGYIKARRQANEQMRQA
ISELNWRPWVEEALTARASNAYDSLRAMPKLGRMPCLANNEVRLLINGAATFEAIFHAIDQAREAVLIQFFIIHDDRLGQ
RLRDLLLKKAAEGVVIHLLYDRIGSHALPHSYVQALRDGGVEVKAFATRSGWLNRFQVNFRNHRKIVVVDGLIGFVGGHN
VGDEYMGEKPPLAPWRDTHVQVRGPVVACMQESFAEDWFWAARSLPPLILPDAYPEDGVLCQLLASGPADAYETCSLFFV
EAIHAATERVWITSPYFIPDEAVFAALRLAVLRGVDVRLLLPSRPDHRIVYAASSLYAFEAVRAGVRVFRYEPGFLHQKV
VLIDSEISAIGSANLDNRSFRLNFEVMLLTVDSEFAASVEQMLNDDFAQAYEVAKEESREIHRLQQVGMRIARLISPIL
>Mature_479_residues
MDYFGPHIFGYLIALIHTLGSIAAIHAVLTVRTAQGSIAWALSLIFIPYLTLIPYLVFGRSTFDGYIKARRQANEQMRQA
ISELNWRPWVEEALTARASNAYDSLRAMPKLGRMPCLANNEVRLLINGAATFEAIFHAIDQAREAVLIQFFIIHDDRLGQ
RLRDLLLKKAAEGVVIHLLYDRIGSHALPHSYVQALRDGGVEVKAFATRSGWLNRFQVNFRNHRKIVVVDGLIGFVGGHN
VGDEYMGEKPPLAPWRDTHVQVRGPVVACMQESFAEDWFWAARSLPPLILPDAYPEDGVLCQLLASGPADAYETCSLFFV
EAIHAATERVWITSPYFIPDEAVFAALRLAVLRGVDVRLLLPSRPDHRIVYAASSLYAFEAVRAGVRVFRYEPGFLHQKV
VLIDSEISAIGSANLDNRSFRLNFEVMLLTVDSEFAASVEQMLNDDFAQAYEVAKEESREIHRLQQVGMRIARLISPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=464, Percent_Identity=26.0775862068966, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1787007, Length=343, Percent_Identity=31.7784256559767, Blast_Score=138, Evalue=9e-34,
Organism=Escherichia coli, GI87081826, Length=402, Percent_Identity=26.6169154228856, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 53800; Mature: 53800

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00435 PEROXIDASE_1 ; PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYFGPHIFGYLIALIHTLGSIAAIHAVLTVRTAQGSIAWALSLIFIPYLTLIPYLVFGR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
STFDGYIKARRQANEQMRQAISELNWRPWVEEALTARASNAYDSLRAMPKLGRMPCLANN
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EVRLLINGAATFEAIFHAIDQAREAVLIQFFIIHDDRLGQRLRDLLLKKAAEGVVIHLLY
CEEEEEECHHHHHHHHHHHHHHHHHEEEEEEEEECHHHHHHHHHHHHHHHCCCEEEEHHH
DRIGSHALPHSYVQALRDGGVEVKAFATRSGWLNRFQVNFRNHRKIVVVDGLIGFVGGHN
HHHCCCCCCHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCEEEEEECHHHHHCCCC
VGDEYMGEKPPLAPWRDTHVQVRGPVVACMQESFAEDWFWAARSLPPLILPDAYPEDGVL
CCHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHH
CQLLASGPADAYETCSLFFVEAIHAATERVWITSPYFIPDEAVFAALRLAVLRGVDVRLL
HHHHHCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEE
LPSRPDHRIVYAASSLYAFEAVRAGVRVFRYEPGFLHQKVVLIDSEISAIGSANLDNRSF
CCCCCCCEEEEEEHHHHHHHHHHHCCEEEEECCCCCCCEEEEEECHHHHHCCCCCCCCEE
RLNFEVMLLTVDSEFAASVEQMLNDDFAQAYEVAKEESREIHRLQQVGMRIARLISPIL
EEEEEEEEEEECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDYFGPHIFGYLIALIHTLGSIAAIHAVLTVRTAQGSIAWALSLIFIPYLTLIPYLVFGR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
STFDGYIKARRQANEQMRQAISELNWRPWVEEALTARASNAYDSLRAMPKLGRMPCLANN
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EVRLLINGAATFEAIFHAIDQAREAVLIQFFIIHDDRLGQRLRDLLLKKAAEGVVIHLLY
CEEEEEECHHHHHHHHHHHHHHHHHEEEEEEEEECHHHHHHHHHHHHHHHCCCEEEEHHH
DRIGSHALPHSYVQALRDGGVEVKAFATRSGWLNRFQVNFRNHRKIVVVDGLIGFVGGHN
HHHCCCCCCHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCEEEEEECHHHHHCCCC
VGDEYMGEKPPLAPWRDTHVQVRGPVVACMQESFAEDWFWAARSLPPLILPDAYPEDGVL
CCHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHH
CQLLASGPADAYETCSLFFVEAIHAATERVWITSPYFIPDEAVFAALRLAVLRGVDVRLL
HHHHHCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEE
LPSRPDHRIVYAASSLYAFEAVRAGVRVFRYEPGFLHQKVVLIDSEISAIGSANLDNRSF
CCCCCCCEEEEEEHHHHHHHHHHHCCEEEEECCCCCCCEEEEEECHHHHHCCCCCCCCEE
RLNFEVMLLTVDSEFAASVEQMLNDDFAQAYEVAKEESREIHRLQQVGMRIARLISPIL
EEEEEEEEEEECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA