Click here to switch to the map view.

The map label for this gene is phoB [H]

Identifier: 77461827

GI number: 77461827

Start: 6277952

End: 6278641

Strand: Direct

Name: phoB [H]

Synonym: Pfl01_5606

Alternate gene names: 77461827

Gene position: 6277952-6278641 (Clockwise)

Preceding gene: 77461825

Following gene: 77461828

Centisome position: 97.51

GC content: 64.78

Gene sequence:

>690_bases
ATGGTTGGCAGGAGCATTCTGATCGTCGACGACGAAGCGCCCATTCGCGAAATGATCGCCGTTGCGTTGGAAATGGCCGG
CTATGACTGCCTCGAGGCAGAGAACTCGCAGCAGGCCCACGCCATCATCGTCGACCGCAAGCCGGACCTGATCCTGCTCG
ACTGGATGCTGCCCGGCACCTCCGGCATCGAGCTGGCCCGCCGCCTCAAGCGTGACGAACTGACCGGGGACATCCCGATC
ATCATGCTCACCGCCAAGGGCGAAGAGGACAACAAGATCCAGGGCCTGGAAGTCGGCGCCGACGACTACATCACCAAACC
GTTTTCCCCCCGCGAACTGGTGGCACGCCTCAAGGCCGTGCTGCGTCGCGCCGGCCCGACCGATGGCGAAGCGCCGATCG
AAGTCGGCGGCCTGCTGCTCGACCCGATCAGCCACCGCGTGACCATCGACGGCCGTCCGGCCGAGATGGGCCCGACCGAA
TACCGTCTGCTGCAATTCTTCATGACCCACCAGGAGCGCGCCTACACCCGTGGCCAGTTGCTGGACCAGGTCTGGGGCGG
CAACGTCTATGTTGAAGAGCGCACCGTCGACGTGCACATCCGGCGCCTGCGCAAGGCCCTCGGCGACGCCTACGAAAATC
TGGTACAAACCGTGCGCGGCACCGGCTACCGGTTCTCCACCAAGGCCTGA

Upstream 100 bases:

>100_bases
ATTCGCACAATCGCCGCCGGACTAAAATACTGTCACACGACTGCAATAATTCCGTTATCTAATGCGGCGCAAGACAGTTA
AATGACAAGAGGATTAAGGC

Downstream 100 bases:

>100_bases
GCCGAGCGCCAGCCCCGTAGACAAGGACCGCATTTTCCGTGAACCAAAACTGGCATGGCACCCTGATCCGCCACATGCTG
TTGCTGGTCACCGGCTGCCT

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MVGRSILIVDDEAPIREMIAVALEMAGYDCLEAENSQQAHAIIVDRKPDLILLDWMLPGTSGIELARRLKRDELTGDIPI
IMLTAKGEEDNKIQGLEVGADDYITKPFSPRELVARLKAVLRRAGPTDGEAPIEVGGLLLDPISHRVTIDGRPAEMGPTE
YRLLQFFMTHQERAYTRGQLLDQVWGGNVYVEERTVDVHIRRLRKALGDAYENLVQTVRGTGYRFSTKA

Sequences:

>Translated_229_residues
MVGRSILIVDDEAPIREMIAVALEMAGYDCLEAENSQQAHAIIVDRKPDLILLDWMLPGTSGIELARRLKRDELTGDIPI
IMLTAKGEEDNKIQGLEVGADDYITKPFSPRELVARLKAVLRRAGPTDGEAPIEVGGLLLDPISHRVTIDGRPAEMGPTE
YRLLQFFMTHQERAYTRGQLLDQVWGGNVYVEERTVDVHIRRLRKALGDAYENLVQTVRGTGYRFSTKA
>Mature_229_residues
MVGRSILIVDDEAPIREMIAVALEMAGYDCLEAENSQQAHAIIVDRKPDLILLDWMLPGTSGIELARRLKRDELTGDIPI
IMLTAKGEEDNKIQGLEVGADDYITKPFSPRELVARLKAVLRRAGPTDGEAPIEVGGLLLDPISHRVTIDGRPAEMGPTE
YRLLQFFMTHQERAYTRGQLLDQVWGGNVYVEERTVDVHIRRLRKALGDAYENLVQTVRGTGYRFSTKA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=228, Percent_Identity=62.280701754386, Blast_Score=291, Evalue=2e-80,
Organism=Escherichia coli, GI1788394, Length=232, Percent_Identity=39.2241379310345, Blast_Score=157, Evalue=4e-40,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=36.6812227074236, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=36.3636363636364, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1786911, Length=224, Percent_Identity=36.6071428571429, Blast_Score=130, Evalue=5e-32,
Organism=Escherichia coli, GI1790863, Length=232, Percent_Identity=35.7758620689655, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI2367329, Length=227, Percent_Identity=37.0044052863436, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI87082012, Length=221, Percent_Identity=33.9366515837104, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1790860, Length=226, Percent_Identity=34.070796460177, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1787375, Length=224, Percent_Identity=29.0178571428571, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=31.1111111111111, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1790552, Length=219, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787229, Length=225, Percent_Identity=34.2222222222222, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI145693140, Length=240, Percent_Identity=32.9166666666667, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=35.1851851851852, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1790299, Length=110, Percent_Identity=32.7272727272727, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788191, Length=121, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25624; Mature: 25624

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGRSILIVDDEAPIREMIAVALEMAGYDCLEAENSQQAHAIIVDRKPDLILLDWMLPGT
CCCCEEEEEECCCCHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEECCCCEEEEEEECCCC
SGIELARRLKRDELTGDIPIIMLTAKGEEDNKIQGLEVGADDYITKPFSPRELVARLKAV
CHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
LRRAGPTDGEAPIEVGGLLLDPISHRVTIDGRPAEMGPTEYRLLQFFMTHQERAYTRGQL
HHHCCCCCCCCCCEECCEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LDQVWGGNVYVEERTVDVHIRRLRKALGDAYENLVQTVRGTGYRFSTKA
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
>Mature Secondary Structure
MVGRSILIVDDEAPIREMIAVALEMAGYDCLEAENSQQAHAIIVDRKPDLILLDWMLPGT
CCCCEEEEEECCCCHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEECCCCEEEEEEECCCC
SGIELARRLKRDELTGDIPIIMLTAKGEEDNKIQGLEVGADDYITKPFSPRELVARLKAV
CHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
LRRAGPTDGEAPIEVGGLLLDPISHRVTIDGRPAEMGPTEYRLLQFFMTHQERAYTRGQL
HHHCCCCCCCCCCEECCEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LDQVWGGNVYVEERTVDVHIRRLRKALGDAYENLVQTVRGTGYRFSTKA
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2115874; 10984043 [H]