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The map label for this gene is spoT [H]

Identifier: 77461772

GI number: 77461772

Start: 6218442

End: 6220547

Strand: Direct

Name: spoT [H]

Synonym: Pfl01_5551

Alternate gene names: 77461772

Gene position: 6218442-6220547 (Clockwise)

Preceding gene: 77461771

Following gene: 77461773

Centisome position: 96.58

GC content: 61.68

Gene sequence:

>2106_bases
ATGCCGAGCATAGACGCCCTCGCCGATCGCTTATCGACCTACCTCGGCAACGACCAGGTCAACCTGGTCCGCCGAGCGTA
TTTCTACGCCGAACAAGCCCACGACGGCCAACGCCGCCGCAGCGGCGAGGCGTACGTCACGCATCCTCTTGCCGTGGCGA
ATATTCTTGCCGACATGCACATGGACCATCAGAGCCTGATGGCTGCGATGCTGCATGACGTGATCGAAGACACCGGTATC
GCCAAGGAAGCGCTGCAAGCGCAGTTCGGTGAAACCGTGGCCGAACTGGTCGACGGGGTCAGCAAACTGACCCAGATGAA
CTTCGAGACCAAGGCCGAAGCCCAGGCTGAAAACTTCCAGAAGATGGCGATGGCGATGGCACGCGACATTCGCGTGATCC
TGGTCAAGCTCGCCGACCGCCTGCACAACATGCGCACGCTGGAAGTGCTGTCCGGCGAGAAACGCCGGCGCATCGCCAAG
GAAACCCTCGAGATCTACGCCCCTATCGCCAACCGGCTGGGCATGCACGCGATCCGCATCGAATTCGAAGACCTCGGCTT
CAAGGCGATGCACCCGATGCGTTCCGCGCGGATCTACCAGGCCGTCAAGCGCGCCCGGGGCAACCGCAAGGAAATCGTCA
ACAAGATCGAAGAATCCCTCGGCCACTGCCTCGCCATCGACGGCATCCAGGGTGAAGTCAGCGGTCGCCAGAAACACCTC
TACGGCATCTACAAGAAAATGCGCGGCAAGCGTCGGGCCTTCAACGAGATCATGGACGTCTATGCGTTCCGGATCATCGT
CGACAAGGTCGATACCTGCTACCGCGTGCTGGGTGCTGTACATAATTTGTACAAACCGTTGCCGGGACGCTTCAAGGATT
ACATCGCGATCCCCAAGGCCAACGGCTATCAGTCGCTGCATACCACGCTGTTCGGCATGCACGGTGTACCGATCGAGATC
CAGATCCGCACCCGCGAAATGGAAGAGATGGCCAACAACGGCATCGCCGCCCATTGGCTGTACAAGTCCAGCGGCGACGA
GCAGCCGAAAGGCACTCACGCCCGCGCCCGTCAGTGGGTCAAGGGCGTGCTGGAAATGCAGCAACGCGCCGGCAACTCGC
TGGAATTCATCGAGAGCGTGAAGATCGACCTGTTCCCGGACGAGGTCTACGTGTTCACGCCCAAAGGCCGGATCATGGAG
CTGCCCAAAGGCTCCACGGCGGTCGACTTTGCCTACGCGGTGCACACCGACGTCGGCAACAGCTGCATCGCCTGTCGGAT
CAACCGTCGTCTCGCGCCGCTGTCCGAACCGCTGCAAAGCGGCTCCACGGTCGAGATCGTCAGCGCTCCTGGCGCACGGC
CGAACCCGGCGTGGCTCAACTTCGTGGTCACCGGCAAGGCGCGGACCCACATCCGCCATGCGCTGAAACTGCAACGCCGC
TCCGAATCCATCAGCCTCGGCGAACGCCTGCTGAACAAGGTGCTCAACGGCTTCGACAGCGCGCTGGAACAGATTCCGGC
CGAACGCGTGAAGGCGATGCTCACCGAGTACCGCCTCGAACTGATCGAAGACCTGCTCGAAGACATCGGACTGGGCAACC
GTATGGCCTATGTCGTGGCGCGCCGCCTGCTCGGCGAAGGCGAACAACTGCCAAGCCCGGAAGGTCCGCTGGCGATTCGC
GGCACCGAAGGCCTGGTTCTCAGCTATGCCAAATGCTGTACGCCGATCCCGGGCGACCCGATTGTCGGTCACCTGTCGGC
GGGCAAAGGCATGGTCGTGCACCTGGACAACTGCCGCAACATCAGCGAAATCCGCCACAACCCGGAAAAATGCATCCAGC
TCTCGTGGGCCAAGGATGTCACCGGCGAATTCAACGTCGAACTGCGCGTCGAGCTGGAACACCAGCGCGGCCTGATCGCC
CTGCTGGCCAGCAGCGTCAACGCAGCCGACGGCAATATCGAGAAAATCAGCATGGACGAACGCGATGGTCGCATCAGCGT
TGTCCAGTTGGTGGTCAGCGTCCACGACCGTGTGCACCTGGCCCGCGTGATCAAGAAACTGCGCGCCCTGACCGGGGTGA
TCCGCATCACCCGCATGCGTGCATAA

Upstream 100 bases:

>100_bases
GGTCTTCGCTGCGTTCGAGGACGAGTCCAACGAGGCCGTCTAAGCCTATGCCTGGTCGACGTAGCACGGCGCGGGATCAC
AGCCTACGGCAGGAGACATC

Downstream 100 bases:

>100_bases
CCACCTAATTACAAGGAGTCATTCATGACCAAAACCGTTATCACCAGCGACAAGGCCCCGGCCGCCATCGGTACTTACTC
TCAGGCGATCAAGGCCGGTA

Product: (p)ppGpp synthetase SpoT/RelA

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 701; Mature: 700

Protein sequence:

>701_residues
MPSIDALADRLSTYLGNDQVNLVRRAYFYAEQAHDGQRRRSGEAYVTHPLAVANILADMHMDHQSLMAAMLHDVIEDTGI
AKEALQAQFGETVAELVDGVSKLTQMNFETKAEAQAENFQKMAMAMARDIRVILVKLADRLHNMRTLEVLSGEKRRRIAK
ETLEIYAPIANRLGMHAIRIEFEDLGFKAMHPMRSARIYQAVKRARGNRKEIVNKIEESLGHCLAIDGIQGEVSGRQKHL
YGIYKKMRGKRRAFNEIMDVYAFRIIVDKVDTCYRVLGAVHNLYKPLPGRFKDYIAIPKANGYQSLHTTLFGMHGVPIEI
QIRTREMEEMANNGIAAHWLYKSSGDEQPKGTHARARQWVKGVLEMQQRAGNSLEFIESVKIDLFPDEVYVFTPKGRIME
LPKGSTAVDFAYAVHTDVGNSCIACRINRRLAPLSEPLQSGSTVEIVSAPGARPNPAWLNFVVTGKARTHIRHALKLQRR
SESISLGERLLNKVLNGFDSALEQIPAERVKAMLTEYRLELIEDLLEDIGLGNRMAYVVARRLLGEGEQLPSPEGPLAIR
GTEGLVLSYAKCCTPIPGDPIVGHLSAGKGMVVHLDNCRNISEIRHNPEKCIQLSWAKDVTGEFNVELRVELEHQRGLIA
LLASSVNAADGNIEKISMDERDGRISVVQLVVSVHDRVHLARVIKKLRALTGVIRITRMRA

Sequences:

>Translated_701_residues
MPSIDALADRLSTYLGNDQVNLVRRAYFYAEQAHDGQRRRSGEAYVTHPLAVANILADMHMDHQSLMAAMLHDVIEDTGI
AKEALQAQFGETVAELVDGVSKLTQMNFETKAEAQAENFQKMAMAMARDIRVILVKLADRLHNMRTLEVLSGEKRRRIAK
ETLEIYAPIANRLGMHAIRIEFEDLGFKAMHPMRSARIYQAVKRARGNRKEIVNKIEESLGHCLAIDGIQGEVSGRQKHL
YGIYKKMRGKRRAFNEIMDVYAFRIIVDKVDTCYRVLGAVHNLYKPLPGRFKDYIAIPKANGYQSLHTTLFGMHGVPIEI
QIRTREMEEMANNGIAAHWLYKSSGDEQPKGTHARARQWVKGVLEMQQRAGNSLEFIESVKIDLFPDEVYVFTPKGRIME
LPKGSTAVDFAYAVHTDVGNSCIACRINRRLAPLSEPLQSGSTVEIVSAPGARPNPAWLNFVVTGKARTHIRHALKLQRR
SESISLGERLLNKVLNGFDSALEQIPAERVKAMLTEYRLELIEDLLEDIGLGNRMAYVVARRLLGEGEQLPSPEGPLAIR
GTEGLVLSYAKCCTPIPGDPIVGHLSAGKGMVVHLDNCRNISEIRHNPEKCIQLSWAKDVTGEFNVELRVELEHQRGLIA
LLASSVNAADGNIEKISMDERDGRISVVQLVVSVHDRVHLARVIKKLRALTGVIRITRMRA
>Mature_700_residues
PSIDALADRLSTYLGNDQVNLVRRAYFYAEQAHDGQRRRSGEAYVTHPLAVANILADMHMDHQSLMAAMLHDVIEDTGIA
KEALQAQFGETVAELVDGVSKLTQMNFETKAEAQAENFQKMAMAMARDIRVILVKLADRLHNMRTLEVLSGEKRRRIAKE
TLEIYAPIANRLGMHAIRIEFEDLGFKAMHPMRSARIYQAVKRARGNRKEIVNKIEESLGHCLAIDGIQGEVSGRQKHLY
GIYKKMRGKRRAFNEIMDVYAFRIIVDKVDTCYRVLGAVHNLYKPLPGRFKDYIAIPKANGYQSLHTTLFGMHGVPIEIQ
IRTREMEEMANNGIAAHWLYKSSGDEQPKGTHARARQWVKGVLEMQQRAGNSLEFIESVKIDLFPDEVYVFTPKGRIMEL
PKGSTAVDFAYAVHTDVGNSCIACRINRRLAPLSEPLQSGSTVEIVSAPGARPNPAWLNFVVTGKARTHIRHALKLQRRS
ESISLGERLLNKVLNGFDSALEQIPAERVKAMLTEYRLELIEDLLEDIGLGNRMAYVVARRLLGEGEQLPSPEGPLAIRG
TEGLVLSYAKCCTPIPGDPIVGHLSAGKGMVVHLDNCRNISEIRHNPEKCIQLSWAKDVTGEFNVELRVELEHQRGLIAL
LASSVNAADGNIEKISMDERDGRISVVQLVVSVHDRVHLARVIKKLRALTGVIRITRMRA

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=700, Percent_Identity=54.1428571428571, Blast_Score=795, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=684, Percent_Identity=32.6023391812866, Blast_Score=391, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25143118, Length=131, Percent_Identity=34.3511450381679, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 78540; Mature: 78409

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSIDALADRLSTYLGNDQVNLVRRAYFYAEQAHDGQRRRSGEAYVTHPLAVANILADMH
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHCCCCCEEECHHHHHHHHHHHC
MDHQSLMAAMLHDVIEDTGIAKEALQAQFGETVAELVDGVSKLTQMNFETKAEAQAENFQ
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
KMAMAMARDIRVILVKLADRLHNMRTLEVLSGEKRRRIAKETLEIYAPIANRLGMHAIRI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEE
EFEDLGFKAMHPMRSARIYQAVKRARGNRKEIVNKIEESLGHCLAIDGIQGEVSGRQKHL
EHHHCCCHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHH
YGIYKKMRGKRRAFNEIMDVYAFRIIVDKVDTCYRVLGAVHNLYKPLPGRFKDYIAIPKA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCC
NGYQSLHTTLFGMHGVPIEIQIRTREMEEMANNGIAAHWLYKSSGDEQPKGTHARARQWV
CCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHH
KGVLEMQQRAGNSLEFIESVKIDLFPDEVYVFTPKGRIMELPKGSTAVDFAYAVHTDVGN
HHHHHHHHHCCCCHHHHHHCEEEEECCCEEEECCCCCEEECCCCCCCEEEEEEEECCCCC
SCIACRINRRLAPLSEPLQSGSTVEIVSAPGARPNPAWLNFVVTGKARTHIRHALKLQRR
CEEEEEECCCCCCHHHHHHCCCEEEEEECCCCCCCCCEEEEEEECCHHHHHHHHHHHHHH
SESISLGERLLNKVLNGFDSALEQIPAERVKAMLTEYRLELIEDLLEDIGLGNRMAYVVA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RRLLGEGEQLPSPEGPLAIRGTEGLVLSYAKCCTPIPGDPIVGHLSAGKGMVVHLDNCRN
HHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCEEEECCCCCEEEEECCCCC
ISEIRHNPEKCIQLSWAKDVTGEFNVELRVELEHQRGLIALLASSVNAADGNIEKISMDE
HHHHHCCHHHHEEEEHHHCCCCCEEEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEECCC
RDGRISVVQLVVSVHDRVHLARVIKKLRALTGVIRITRMRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSIDALADRLSTYLGNDQVNLVRRAYFYAEQAHDGQRRRSGEAYVTHPLAVANILADMH
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHCCCCCEEECHHHHHHHHHHHC
MDHQSLMAAMLHDVIEDTGIAKEALQAQFGETVAELVDGVSKLTQMNFETKAEAQAENFQ
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
KMAMAMARDIRVILVKLADRLHNMRTLEVLSGEKRRRIAKETLEIYAPIANRLGMHAIRI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEE
EFEDLGFKAMHPMRSARIYQAVKRARGNRKEIVNKIEESLGHCLAIDGIQGEVSGRQKHL
EHHHCCCHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHH
YGIYKKMRGKRRAFNEIMDVYAFRIIVDKVDTCYRVLGAVHNLYKPLPGRFKDYIAIPKA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCC
NGYQSLHTTLFGMHGVPIEIQIRTREMEEMANNGIAAHWLYKSSGDEQPKGTHARARQWV
CCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHH
KGVLEMQQRAGNSLEFIESVKIDLFPDEVYVFTPKGRIMELPKGSTAVDFAYAVHTDVGN
HHHHHHHHHCCCCHHHHHHCEEEEECCCEEEECCCCCEEECCCCCCCEEEEEEEECCCCC
SCIACRINRRLAPLSEPLQSGSTVEIVSAPGARPNPAWLNFVVTGKARTHIRHALKLQRR
CEEEEEECCCCCCHHHHHHCCCEEEEEECCCCCCCCCEEEEEEECCHHHHHHHHHHHHHH
SESISLGERLLNKVLNGFDSALEQIPAERVKAMLTEYRLELIEDLLEDIGLGNRMAYVVA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RRLLGEGEQLPSPEGPLAIRGTEGLVLSYAKCCTPIPGDPIVGHLSAGKGMVVHLDNCRN
HHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCEEEECCCCCEEEEECCCCC
ISEIRHNPEKCIQLSWAKDVTGEFNVELRVELEHQRGLIALLASSVNAADGNIEKISMDE
HHHHHCCHHHHEEEEHHHCCCCCEEEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEECCC
RDGRISVVQLVVSVHDRVHLARVIKKLRALTGVIRITRMRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]