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The map label for this gene is exoA [H]

Identifier: 77461766

GI number: 77461766

Start: 6214225

End: 6215004

Strand: Direct

Name: exoA [H]

Synonym: Pfl01_5545

Alternate gene names: 77461766

Gene position: 6214225-6215004 (Clockwise)

Preceding gene: 77461764

Following gene: 77461769

Centisome position: 96.52

GC content: 59.36

Gene sequence:

>780_bases
ATGCGGATCATCAGTGTGAACGTCAATGGTATTCAGGCTGCAGTCGAGCGCGGTTTGCTCAGTTGGCTGCAAGCACAGAA
TGCCGACGTCATCTGCCTGCAGGACACCCGTGCCTCCGCCTTTGAACTGGATGACCCAGCCTTCCAACTGGACGGCTACT
TCCTTTATGCCTGCGATGCTGAAGTCCCTGCCCAAGGCGGCGTGGCTTTGTATTCGCGGTTGCAACCGAAGGCTGTCATC
AGCGGTCTCGGTTTCGAGACGGCCGACCGCTACGGGCGCTACCTGCAAGCAGATTTCGACAAAGTCAGTATTGCCACCTT
GCTGCTTCCTTCGGGGATGAACGGCGATGAGGATTTGAACCAGAAGTTCAAGCTCATGGACGACTTCGGCAAGTACCTGG
ACAAACAGCGGCGCAAACGTCGCGAGTACATTTATTGTGGCTCGCTGTACGTCGCGCAGCAGAAGCTCGACATCAAGAAC
TGGCGCGACAGCCAGCAATCCCCGGGCTTCCTGGCGCCGGAGCGCGCCTGGATGGACGAGATTGTCGGCAACATGGGCTA
CGTAGATGCCCTGCGCGAAGTCAGCCGCGAAGGCGACCAGTACAGCTGGTGGCCGGACAACGAACAGGCCGAGATGTTGA
ATCTGGGCTGGCGCTTCGACTACCAGATCCTGACTCCGGGACTGCGGCGCTTCGTGCGCAGCGCACGCCTGCCACGTCAG
CCACGGTTCTCGCAGCATGCACCGCTGATCGTCGACTACGACTGGACGCTGACCATCTGA

Upstream 100 bases:

>100_bases
GCGCTGATACGCTTGCATGAAAAAAACCCCAAATACCACGGATTTAGCTAATTAGCTTGACGCCGTGTATCATACACGCA
CGCGATTTTTGGGGCCATTT

Downstream 100 bases:

>100_bases
GCGGCCTTTCGCAGACACAAAAAAACCGACATCACTGTCGGTTTTTTTGTGGGCGATCGTTCGTTATTTGATCAACCGCC
AGGTGAACGGATAGCGGTAA

Product: exodeoxyribonuclease III xth

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MRIISVNVNGIQAAVERGLLSWLQAQNADVICLQDTRASAFELDDPAFQLDGYFLYACDAEVPAQGGVALYSRLQPKAVI
SGLGFETADRYGRYLQADFDKVSIATLLLPSGMNGDEDLNQKFKLMDDFGKYLDKQRRKRREYIYCGSLYVAQQKLDIKN
WRDSQQSPGFLAPERAWMDEIVGNMGYVDALREVSREGDQYSWWPDNEQAEMLNLGWRFDYQILTPGLRRFVRSARLPRQ
PRFSQHAPLIVDYDWTLTI

Sequences:

>Translated_259_residues
MRIISVNVNGIQAAVERGLLSWLQAQNADVICLQDTRASAFELDDPAFQLDGYFLYACDAEVPAQGGVALYSRLQPKAVI
SGLGFETADRYGRYLQADFDKVSIATLLLPSGMNGDEDLNQKFKLMDDFGKYLDKQRRKRREYIYCGSLYVAQQKLDIKN
WRDSQQSPGFLAPERAWMDEIVGNMGYVDALREVSREGDQYSWWPDNEQAEMLNLGWRFDYQILTPGLRRFVRSARLPRQ
PRFSQHAPLIVDYDWTLTI
>Mature_259_residues
MRIISVNVNGIQAAVERGLLSWLQAQNADVICLQDTRASAFELDDPAFQLDGYFLYACDAEVPAQGGVALYSRLQPKAVI
SGLGFETADRYGRYLQADFDKVSIATLLLPSGMNGDEDLNQKFKLMDDFGKYLDKQRRKRREYIYCGSLYVAQQKLDIKN
WRDSQQSPGFLAPERAWMDEIVGNMGYVDALREVSREGDQYSWWPDNEQAEMLNLGWRFDYQILTPGLRRFVRSARLPRQ
PRFSQHAPLIVDYDWTLTI

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=254, Percent_Identity=27.5590551181102, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI18375503, Length=254, Percent_Identity=27.5590551181102, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI18375501, Length=254, Percent_Identity=27.5590551181102, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71989536, Length=259, Percent_Identity=24.3243243243243, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI221330655, Length=258, Percent_Identity=28.2945736434109, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17136678, Length=239, Percent_Identity=29.7071129707113, Blast_Score=101, Evalue=6e-22,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29786; Mature: 29786

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIISVNVNGIQAAVERGLLSWLQAQNADVICLQDTRASAFELDDPAFQLDGYFLYACDA
CEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCEEECCEEEEEECC
EVPAQGGVALYSRLQPKAVISGLGFETADRYGRYLQADFDKVSIATLLLPSGMNGDEDLN
CCCCCCCHHHHHHCCHHHHHHCCCCHHHHHHHHHHHCCCCHHEEEEEEECCCCCCCHHHH
QKFKLMDDFGKYLDKQRRKRREYIYCGSLYVAQQKLDIKNWRDSQQSPGFLAPERAWMDE
HHHHHHHHHHHHHHHHHHHHHCEEEECCEEEEHHHHCHHHCCCCCCCCCCCCCHHHHHHH
IVGNMGYVDALREVSREGDQYSWWPDNEQAEMLNLGWRFDYQILTPGLRRFVRSARLPRQ
HHCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHEECCCEEEHEEECHHHHHHHHHHCCCCC
PRFSQHAPLIVDYDWTLTI
CCCCCCCCEEEECCEEEEC
>Mature Secondary Structure
MRIISVNVNGIQAAVERGLLSWLQAQNADVICLQDTRASAFELDDPAFQLDGYFLYACDA
CEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCEEECCEEEEEECC
EVPAQGGVALYSRLQPKAVISGLGFETADRYGRYLQADFDKVSIATLLLPSGMNGDEDLN
CCCCCCCHHHHHHCCHHHHHHCCCCHHHHHHHHHHHCCCCHHEEEEEEECCCCCCCHHHH
QKFKLMDDFGKYLDKQRRKRREYIYCGSLYVAQQKLDIKNWRDSQQSPGFLAPERAWMDE
HHHHHHHHHHHHHHHHHHHHHCEEEECCEEEEHHHHCHHHCCCCCCCCCCCCCHHHHHHH
IVGNMGYVDALREVSREGDQYSWWPDNEQAEMLNLGWRFDYQILTPGLRRFVRSARLPRQ
HHCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHEECCCEEEHEEECHHHHHHHHHHCCCCC
PRFSQHAPLIVDYDWTLTI
CCCCCCCCEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]