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The map label for this gene is puuB [H]

Identifier: 77461750

GI number: 77461750

Start: 6197571

End: 6198881

Strand: Direct

Name: puuB [H]

Synonym: Pfl01_5529

Alternate gene names: 77461750

Gene position: 6197571-6198881 (Clockwise)

Preceding gene: 77461748

Following gene: 77461752

Centisome position: 96.26

GC content: 63.77

Gene sequence:

>1311_bases
ATGAATGCCCGCGCCCAAGCCACCGCGAGCCAACCCCACGTTGCCTCTTATTACGCCGCCAGCAGCTTGCCGCAGCCCGA
CCATCCGGTGCTGAAAGGTGAACTGATCGCCGACGTGTGCGTGGTCGGTGGAGGTTTCTCCGGGCTGAACACGGCGCTGG
AATTGGCTGAACGGGGTTTGAGCGTGGCGCTGCTGGAAGCGCACCGGATCGGCTGGGGCGCCAGCGGTCGCAACGGCGGG
CAACTGATTCGCGGGGTCGGTCACGGCCTCGATCAGTTCGCCAACGTGATCGGCGCCGACGGCGTGCGTCAGATGAAGCT
CATGGGCCTGGAAGCGGTGGAAATCGTCCGCCAGCGCGTCGAAAAATTTCAGATCCCCTGCGACCTGACCTGGGGTTACT
GCGACCTCGCCAACAGACCTTCCGACCTTGAAGGTTTTGCCGAAGACGCCGAAGAGCTGCGCAGCCTCGGTTACCGCTAC
GAAACCCGACTGCTGCAAGCGAACGAAATGCACACTGTGGTCGGCTCCAGACGCTATGTCGGCGGTCTGATCGACATGGG
CTCAGGGCATCTGCACCCGTTGAACCTGGCACTCGGCGAAGCCGCAGCGGCGCAACAACTCGGGGTGAAGCTGTTCGAGC
AATCGGCGGTCACCCGCATCGACTACGGCCCCGAAGTCCGCGTACATACGGCGCAAGGTTCGGTGCGGGCAAAAACCCTG
GTGCTCGGCTGCAACGCCTATCTCAATGGGCTGAATCCGCAACTCAGCGGCAAGGTACTGCCGGCCGGCAGCTACATCAT
TGCCACCGAACCGCTGAGCGAAGAGCAGGCCCACGCCCTGCTGCCGCAGAACATGGCGGTCTGCGATCAGCGGGTGGCGC
TCGATTACTACCGGCTCTCGGCCGACCGCCGCCTGCTGTTTGGCGGCGCCTGCCATTACTCGGGGCGCGATCCGAAGGAC
ATCGGCGCGTACATGCGGCCGAAGATGCTTGAAGTGTTCCCGCAGCTCGCGTCAGCGAAGATCGATTATCAGTGGGGCGG
GATGATCGGCATCGGTGCCAACCGTCTGCCGCAGATTGGCCGGCTCGCCGATCAGCCGAATGTGTATTACGCCCAGGCCT
ATTCCGGCCACGGCGTGAATGCCACGCACCTGGCAGGCAAGCTGCTGGCCGAGGCCATCAGCGGCCAACACAGCGGCGGC
TTCGACCTGTTCGCCAAAGTGCCACACATCACCTTCCCCGGCGGCAAGCATCTGCGCTCGCCGCTGCTGGCGTTGGGGAT
GTTGTGGCATCGGCTTAAAGAGCTGGTCTGA

Upstream 100 bases:

>100_bases
GCCGTGATCCAGACACCACGGGCCGACCAATGTTGCCCAGCCTCGGGCCTGCGGCTTATGCTCCGTCAAATTTTCTCGAC
ACCCGCTCAGGATGACGCCC

Downstream 100 bases:

>100_bases
GATCGTTCCCACGCTCCTGCGTGGGAACGCTCCTTTCTAGACCCGCCAGAACGGCTTCAACCCCTCCTCCAGCGCCTGCT
CCCGGGTCAACCCGATGTCC

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MNARAQATASQPHVASYYAASSLPQPDHPVLKGELIADVCVVGGGFSGLNTALELAERGLSVALLEAHRIGWGASGRNGG
QLIRGVGHGLDQFANVIGADGVRQMKLMGLEAVEIVRQRVEKFQIPCDLTWGYCDLANRPSDLEGFAEDAEELRSLGYRY
ETRLLQANEMHTVVGSRRYVGGLIDMGSGHLHPLNLALGEAAAAQQLGVKLFEQSAVTRIDYGPEVRVHTAQGSVRAKTL
VLGCNAYLNGLNPQLSGKVLPAGSYIIATEPLSEEQAHALLPQNMAVCDQRVALDYYRLSADRRLLFGGACHYSGRDPKD
IGAYMRPKMLEVFPQLASAKIDYQWGGMIGIGANRLPQIGRLADQPNVYYAQAYSGHGVNATHLAGKLLAEAISGQHSGG
FDLFAKVPHITFPGGKHLRSPLLALGMLWHRLKELV

Sequences:

>Translated_436_residues
MNARAQATASQPHVASYYAASSLPQPDHPVLKGELIADVCVVGGGFSGLNTALELAERGLSVALLEAHRIGWGASGRNGG
QLIRGVGHGLDQFANVIGADGVRQMKLMGLEAVEIVRQRVEKFQIPCDLTWGYCDLANRPSDLEGFAEDAEELRSLGYRY
ETRLLQANEMHTVVGSRRYVGGLIDMGSGHLHPLNLALGEAAAAQQLGVKLFEQSAVTRIDYGPEVRVHTAQGSVRAKTL
VLGCNAYLNGLNPQLSGKVLPAGSYIIATEPLSEEQAHALLPQNMAVCDQRVALDYYRLSADRRLLFGGACHYSGRDPKD
IGAYMRPKMLEVFPQLASAKIDYQWGGMIGIGANRLPQIGRLADQPNVYYAQAYSGHGVNATHLAGKLLAEAISGQHSGG
FDLFAKVPHITFPGGKHLRSPLLALGMLWHRLKELV
>Mature_436_residues
MNARAQATASQPHVASYYAASSLPQPDHPVLKGELIADVCVVGGGFSGLNTALELAERGLSVALLEAHRIGWGASGRNGG
QLIRGVGHGLDQFANVIGADGVRQMKLMGLEAVEIVRQRVEKFQIPCDLTWGYCDLANRPSDLEGFAEDAEELRSLGYRY
ETRLLQANEMHTVVGSRRYVGGLIDMGSGHLHPLNLALGEAAAAQQLGVKLFEQSAVTRIDYGPEVRVHTAQGSVRAKTL
VLGCNAYLNGLNPQLSGKVLPAGSYIIATEPLSEEQAHALLPQNMAVCDQRVALDYYRLSADRRLLFGGACHYSGRDPKD
IGAYMRPKMLEVFPQLASAKIDYQWGGMIGIGANRLPQIGRLADQPNVYYAQAYSGHGVNATHLAGKLLAEAISGQHSGG
FDLFAKVPHITFPGGKHLRSPLLALGMLWHRLKELV

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=423, Percent_Identity=44.9172576832151, Blast_Score=362, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47017; Mature: 47017

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNARAQATASQPHVASYYAASSLPQPDHPVLKGELIADVCVVGGGFSGLNTALELAERGL
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
SVALLEAHRIGWGASGRNGGQLIRGVGHGLDQFANVIGADGVRQMKLMGLEAVEIVRQRV
EEEEEEHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EKFQIPCDLTWGYCDLANRPSDLEGFAEDAEELRSLGYRYETRLLQANEMHTVVGSRRYV
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHCCCHHH
GGLIDMGSGHLHPLNLALGEAAAAQQLGVKLFEQSAVTRIDYGPEVRVHTAQGSVRAKTL
CCEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCEEEEE
VLGCNAYLNGLNPQLSGKVLPAGSYIIATEPLSEEQAHALLPQNMAVCDQRVALDYYRLS
EEECHHHHCCCCCCCCCEEECCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHC
ADRRLLFGGACHYSGRDPKDIGAYMRPKMLEVFPQLASAKIDYQWGGMIGIGANRLPQIG
CCCEEEEECEECCCCCCHHHHHHHHCHHHHHHHHHHHCCEEEEEECCEEECCCCCCCHHH
RLADQPNVYYAQAYSGHGVNATHLAGKLLAEAISGQHSGGFDLFAKVPHITFPGGKHLRS
HHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCEEECCCCHHHHH
PLLALGMLWHRLKELV
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNARAQATASQPHVASYYAASSLPQPDHPVLKGELIADVCVVGGGFSGLNTALELAERGL
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
SVALLEAHRIGWGASGRNGGQLIRGVGHGLDQFANVIGADGVRQMKLMGLEAVEIVRQRV
EEEEEEHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EKFQIPCDLTWGYCDLANRPSDLEGFAEDAEELRSLGYRYETRLLQANEMHTVVGSRRYV
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHCCCHHH
GGLIDMGSGHLHPLNLALGEAAAAQQLGVKLFEQSAVTRIDYGPEVRVHTAQGSVRAKTL
CCEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCEEEEE
VLGCNAYLNGLNPQLSGKVLPAGSYIIATEPLSEEQAHALLPQNMAVCDQRVALDYYRLS
EEECHHHHCCCCCCCCCEEECCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHC
ADRRLLFGGACHYSGRDPKDIGAYMRPKMLEVFPQLASAKIDYQWGGMIGIGANRLPQIG
CCCEEEEECEECCCCCCHHHHHHHHCHHHHHHHHHHHCCEEEEEECCEEECCCCCCCHHH
RLADQPNVYYAQAYSGHGVNATHLAGKLLAEAISGQHSGGFDLFAKVPHITFPGGKHLRS
HHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCEEECCCCHHHHH
PLLALGMLWHRLKELV
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]