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The map label for this gene is argH2

Identifier: 77461710

GI number: 77461710

Start: 6156949

End: 6158343

Strand: Direct

Name: argH2

Synonym: Pfl01_5489

Alternate gene names: 77461710

Gene position: 6156949-6158343 (Clockwise)

Preceding gene: 77461702

Following gene: 77461712

Centisome position: 95.63

GC content: 62.94

Gene sequence:

>1395_bases
ATGAGCACTGACAAGACCAATCAGTCCTGGGGCGGCCGCTTCAGTGAACCCGTCGACGCCTTCGTCGCCCGCTTCACCGC
CTCCGTCACTTTCGACCAGCGCCTGTATCGCCACGACATCATGGGCTCGATCGCCCACGCCACCATGCTGGCCAAGGTCG
GCGTGCTGACGGATGCCGAGCGCGACAGCATCATCGATGGCCTGAAGACCATCCAGGGCGAAATCGAGGCCGGCCAGTTT
GACTGGCGCGTCGACCTCGAAGACGTGCACATGAACATCGAAGCACGCCTGACCGACCGCATCGGCGTCACCGGCAAGAA
GCTGCACACCGGCCGTAGCCGCAACGACCAGGTCGCCACCGACATCCGCCTGTGGCTGCGTGACGAAATCGACCTGATCC
TGAGCGAAATCACCCGCCTGCAAAAAGGCCTGCTGGAGCAGGCCGAGCGTGAAGCCGCCAGCATCATGCCGGGCTTTACT
CACCTGCAGACCGCTCAGCCGGTGACCTTCGGGCACCACATGCTGGCCTGGTTCGAAATGCTCAGCCGCGACTACGAGCG
TCTGGTCGACTGCCGCAAGCGCACCAACCGCATGCCGCTGGGCAGCGCCGCACTGGCCGGCACCACTTACCCGATCGACC
GCGAATACACCGCACAACTGCTGGGCTTCGACGCCGTCGGCGGCAACTCGCTGGACAACGTGTCCGACCGTGACTTCGCC
ATCGAATTCTGCTCGGCCGCGAGCATCGCGATGATGCACCTGTCGCGCTTCTCCGAAGAGCTGGTGCTGTGGACCAGCGC
GCAATTCCAGTTCATCGATCTGCCAGACCGTTTCTGCACCGGCAGCTCGATCATGCCGCAAAAGAAAAACCCCGACGTGC
CAGAGCTGGTACGTGGCAAGACCGGCCGTGTGTTCGGCGCCCTGATGGGCCTGTTGACCCTGATGAAAGGCCAGCCACTG
GCCTACAACAAGGACAATCAGGAAGACAAGGAACCGTTGTTCGACGCCGCCGATACCCTGCGCGACTCGCTGCGGGCTTT
TGCCGACATGATCCCGGCGATCAAGCCAAAGCACGCGATCATGCGTGAAGCGGCACTGCGCGGTTTCTCCACCGCCACCG
ACCTGGCCGACTACCTGGTACGCCGTGGCCTGCCGTTCCGTGATTGCCACGAGATCGTCGGTCATGCCGTGAAGTACGGC
GTCGACACCGGCAAGGATCTGGCGGAAATGAGCCTGGAAGAACTGCGTCAGTTCAGCGACCAGATCGAGCAGGACGTGTT
TGCAGTGCTGACCCTGGAAGGTTCGGTAAATGCCCGTGACCACATCGGCGGCACTGCGCCGGCGCAGGTCAAGGCTGCCG
TGGTTCGTGGTCAGGCGTTGATCGCCAGCCGCTAA

Upstream 100 bases:

>100_bases
AAAAACGTATCGACCAGTGTCAGCGACAGGATTGGCAACCCTGTTATTATCCGCGCCAGCGTTTCACGCCTTCTTTCTTC
AAGCCGATACGAGCGAATTC

Downstream 100 bases:

>100_bases
AAGCATCGCGGGCAAGTCGAGTTTTCGACTTGCCCGCGAACTTGCTCAAGGTTTTCTACTTTTTGGCGGCGATCATCGCC
AAAAACGCCGGCATCGCCGC

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL 2; Arginosuccinase 2

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSTDKTNQSWGGRFSEPVDAFVARFTASVTFDQRLYRHDIMGSIAHATMLAKVGVLTDAERDSIIDGLKTIQGEIEAGQF
DWRVDLEDVHMNIEARLTDRIGVTGKKLHTGRSRNDQVATDIRLWLRDEIDLILSEITRLQKGLLEQAEREAASIMPGFT
HLQTAQPVTFGHHMLAWFEMLSRDYERLVDCRKRTNRMPLGSAALAGTTYPIDREYTAQLLGFDAVGGNSLDNVSDRDFA
IEFCSAASIAMMHLSRFSEELVLWTSAQFQFIDLPDRFCTGSSIMPQKKNPDVPELVRGKTGRVFGALMGLLTLMKGQPL
AYNKDNQEDKEPLFDAADTLRDSLRAFADMIPAIKPKHAIMREAALRGFSTATDLADYLVRRGLPFRDCHEIVGHAVKYG
VDTGKDLAEMSLEELRQFSDQIEQDVFAVLTLEGSVNARDHIGGTAPAQVKAAVVRGQALIASR

Sequences:

>Translated_464_residues
MSTDKTNQSWGGRFSEPVDAFVARFTASVTFDQRLYRHDIMGSIAHATMLAKVGVLTDAERDSIIDGLKTIQGEIEAGQF
DWRVDLEDVHMNIEARLTDRIGVTGKKLHTGRSRNDQVATDIRLWLRDEIDLILSEITRLQKGLLEQAEREAASIMPGFT
HLQTAQPVTFGHHMLAWFEMLSRDYERLVDCRKRTNRMPLGSAALAGTTYPIDREYTAQLLGFDAVGGNSLDNVSDRDFA
IEFCSAASIAMMHLSRFSEELVLWTSAQFQFIDLPDRFCTGSSIMPQKKNPDVPELVRGKTGRVFGALMGLLTLMKGQPL
AYNKDNQEDKEPLFDAADTLRDSLRAFADMIPAIKPKHAIMREAALRGFSTATDLADYLVRRGLPFRDCHEIVGHAVKYG
VDTGKDLAEMSLEELRQFSDQIEQDVFAVLTLEGSVNARDHIGGTAPAQVKAAVVRGQALIASR
>Mature_463_residues
STDKTNQSWGGRFSEPVDAFVARFTASVTFDQRLYRHDIMGSIAHATMLAKVGVLTDAERDSIIDGLKTIQGEIEAGQFD
WRVDLEDVHMNIEARLTDRIGVTGKKLHTGRSRNDQVATDIRLWLRDEIDLILSEITRLQKGLLEQAEREAASIMPGFTH
LQTAQPVTFGHHMLAWFEMLSRDYERLVDCRKRTNRMPLGSAALAGTTYPIDREYTAQLLGFDAVGGNSLDNVSDRDFAI
EFCSAASIAMMHLSRFSEELVLWTSAQFQFIDLPDRFCTGSSIMPQKKNPDVPELVRGKTGRVFGALMGLLTLMKGQPLA
YNKDNQEDKEPLFDAADTLRDSLRAFADMIPAIKPKHAIMREAALRGFSTATDLADYLVRRGLPFRDCHEIVGHAVKYGV
DTGKDLAEMSLEELRQFSDQIEQDVFAVLTLEGSVNARDHIGGTAPAQVKAAVVRGQALIASR

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily

Homologues:

Organism=Homo sapiens, GI31541964, Length=460, Percent_Identity=43.2608695652174, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI68303542, Length=460, Percent_Identity=43.2608695652174, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI68303547, Length=460, Percent_Identity=40.8695652173913, Blast_Score=341, Evalue=1e-93,
Organism=Homo sapiens, GI68303549, Length=460, Percent_Identity=40.4347826086956, Blast_Score=333, Evalue=2e-91,
Organism=Escherichia coli, GI1790398, Length=451, Percent_Identity=49.2239467849224, Blast_Score=438, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6321806, Length=452, Percent_Identity=43.5840707964602, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI221473854, Length=441, Percent_Identity=40.1360544217687, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI78706858, Length=441, Percent_Identity=40.1360544217687, Blast_Score=326, Evalue=2e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARLY2_PSEPF (Q3K4S8)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_351217.1
- ProteinModelPortal:   Q3K4S8
- SMR:   Q3K4S8
- STRING:   Q3K4S8
- GeneID:   3713813
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_5489
- eggNOG:   COG0165
- HOGENOM:   HBG539632
- OMA:   EDIHTVI
- ProtClustDB:   PRK00855
- BioCyc:   PFLU205922:PFL_5489-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00006
- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PANTHER:   PTHR11444:SF3
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00838

Pfam domain/function: PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.1

Molecular weight: Translated: 51682; Mature: 51551

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDKTNQSWGGRFSEPVDAFVARFTASVTFDQRLYRHDIMGSIAHATMLAKVGVLTDAE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
RDSIIDGLKTIQGEIEAGQFDWRVDLEDVHMNIEARLTDRIGVTGKKLHTGRSRNDQVAT
HHHHHHHHHHHHCCCCCCCEEEEEEHHHHHCCEEEEEHHHCCCCCCHHCCCCCCCCHHHH
DIRLWLRDEIDLILSEITRLQKGLLEQAEREAASIMPGFTHLQTAQPVTFGHHMLAWFEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHH
LSRDYERLVDCRKRTNRMPLGSAALAGTTYPIDREYTAQLLGFDAVGGNSLDNVSDRDFA
HHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHH
IEFCSAASIAMMHLSRFSEELVLWTSAQFQFIDLPDRFCTGSSIMPQKKNPDVPELVRGK
HHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCHHHCCCCCCCCCCCCCCCHHHHCCC
TGRVFGALMGLLTLMKGQPLAYNKDNQEDKEPLFDAADTLRDSLRAFADMIPAIKPKHAI
CCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
MREAALRGFSTATDLADYLVRRGLPFRDCHEIVGHAVKYGVDTGKDLAEMSLEELRQFSD
HHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
QIEQDVFAVLTLEGSVNARDHIGGTAPAQVKAAVVRGQALIASR
HHHHHEEEEEEECCCCCCHHCCCCCCCHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
STDKTNQSWGGRFSEPVDAFVARFTASVTFDQRLYRHDIMGSIAHATMLAKVGVLTDAE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
RDSIIDGLKTIQGEIEAGQFDWRVDLEDVHMNIEARLTDRIGVTGKKLHTGRSRNDQVAT
HHHHHHHHHHHHCCCCCCCEEEEEEHHHHHCCEEEEEHHHCCCCCCHHCCCCCCCCHHHH
DIRLWLRDEIDLILSEITRLQKGLLEQAEREAASIMPGFTHLQTAQPVTFGHHMLAWFEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHH
LSRDYERLVDCRKRTNRMPLGSAALAGTTYPIDREYTAQLLGFDAVGGNSLDNVSDRDFA
HHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHH
IEFCSAASIAMMHLSRFSEELVLWTSAQFQFIDLPDRFCTGSSIMPQKKNPDVPELVRGK
HHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCHHHCCCCCCCCCCCCCCCHHHHCCC
TGRVFGALMGLLTLMKGQPLAYNKDNQEDKEPLFDAADTLRDSLRAFADMIPAIKPKHAI
CCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
MREAALRGFSTATDLADYLVRRGLPFRDCHEIVGHAVKYGVDTGKDLAEMSLEELRQFSD
HHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
QIEQDVFAVLTLEGSVNARDHIGGTAPAQVKAAVVRGQALIASR
HHHHHEEEEEEECCCCCCHHCCCCCCCHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA