Click here to switch to the map view.

The map label for this gene is puuA [H]

Identifier: 77461628

GI number: 77461628

Start: 6070936

End: 6072294

Strand: Reverse

Name: puuA [H]

Synonym: Pfl01_5407

Alternate gene names: 77461628

Gene position: 6072294-6070936 (Counterclockwise)

Preceding gene: 77461631

Following gene: 77461627

Centisome position: 94.31

GC content: 62.03

Gene sequence:

>1359_bases
ATGAGTAACAACCTCGACCAGCTCACCGATTGGTTGAAAGACCACAAGATCACAGAAGTCGAATGCATGATCGGCGACTT
GACCGGCATCACCCGGGGCAAGATCTCGCCGACCAACAAGTTCATCGCCGAAAAAGGCATGCGCCTGCCAGAGAGCGTTC
TGTTGCAGACCGTGACCGGCGACTACGTCGAAGACGACATCTATTACGAACTGCTCGACCCGGCCGACATCGACATGATC
TGCCGCCCCGACCAGAACGCCGTGTACCTGGTGCCGTGGGCCATCGAGCCGACCGCCCAGGTGATCCACGATACCTACGA
CAAGCAAGGCAACCCGATCGAGCTGTCGCCGCGCAACGTCCTCAAGAAAGTCCTGAAACTCTATGCCGACAAGGGCTGGC
AGCCGATCGTGGCGCCGGAAATGGAGTTCTACCTGACCAAGCGCAGCGACGACCCTGACTATCCGTTGCAGCCGCCAGTC
GGCCGCTCCGGGCGTCCGGAGATCGGTCGTCAGTCGTTCTCCATCGAAGCGGCGAACGAATTCGATCCGCTGTTCGAAGA
CGTCTACGACTGGTGCGAATTGCAGGAACTGGACCTCGACACGCTGATCCACGAGGACGGCACGGCGCAGATGGAAATCA
ACTTCCGTCACGGCGATGCCCTGTCGCTGGCCGACCAGATCCTGGTGTTCAAGCGCACCATGCGCGAGGCCGCCCTCAAG
CACAACGTGGCCGCGACCTTCATGGCCAAGCCGATGACCGGCGAGCCGGGCAGTGCGATGCACCTGCACCAGAGCATCAT
CGACATCGAGACTGGCAAAAACGTGTTCTCCAATGACGACGGGACCATGAGCCAGCTGTTCCTGCACCACATCGGTGGCC
TGCAGAAACTGATCCCTGAGCTGTTGCCGCTGTTCGCACCGAACGTCAACTCGTTCCGCCGCTTCCTGCCGGACACCTCG
GCGCCGGTGAACGTGGAGTGGGGCGAAGAAAACCGCACCGTGGGCCTGCGCGTACCGGATGCCGGCCCGCAGAACCGCCG
GGTGGAAAACCGCCTGCCGGGCGCCGACGCCAACCCGTACCTGGCGATTGCCGCGAGCCTGCTCTGCGGCTACATCGGCA
TGGTCGAAGGCATCAACCCGAGCGCGCCGGTCGTGGGCCGTGGTTACGAGCGCCGCAACCTGCGCCTGCCGCTGACCATC
GAGGACGCGCTGGAGCGTATGGAAAACAGCGCGACCATCGAGAAGTATCTGGGCAAGACCTTCATCACCGGCTACGTCGC
GGTCAAGCGGGCCGAGCATGAAAACTTCAAGCGCGTGATCAGTTCCTGGGAGCGTGAGTACCTGCTCTTCGCCGTCTGA

Upstream 100 bases:

>100_bases
GCGTAAGCCGGCACTGCGCGCAAGCAAGGCCCTTTAACGCGACGCCTGGTGCGTCAAACATTGCCTGACATCTGTCAGGA
GACTCAGCCCAGAGGCATTT

Downstream 100 bases:

>100_bases
TACGCCGGGCGCGGTGGCCAGCCGCCGCGCCTTCACCGATAACAAGGAGAATTGGCATGACCAGCAACAACCCGCAAACC
CGTGAGTGGCAAGCCCTCAG

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSNNLDQLTDWLKDHKITEVECMIGDLTGITRGKISPTNKFIAEKGMRLPESVLLQTVTGDYVEDDIYYELLDPADIDMI
CRPDQNAVYLVPWAIEPTAQVIHDTYDKQGNPIELSPRNVLKKVLKLYADKGWQPIVAPEMEFYLTKRSDDPDYPLQPPV
GRSGRPEIGRQSFSIEAANEFDPLFEDVYDWCELQELDLDTLIHEDGTAQMEINFRHGDALSLADQILVFKRTMREAALK
HNVAATFMAKPMTGEPGSAMHLHQSIIDIETGKNVFSNDDGTMSQLFLHHIGGLQKLIPELLPLFAPNVNSFRRFLPDTS
APVNVEWGEENRTVGLRVPDAGPQNRRVENRLPGADANPYLAIAASLLCGYIGMVEGINPSAPVVGRGYERRNLRLPLTI
EDALERMENSATIEKYLGKTFITGYVAVKRAEHENFKRVISSWEREYLLFAV

Sequences:

>Translated_452_residues
MSNNLDQLTDWLKDHKITEVECMIGDLTGITRGKISPTNKFIAEKGMRLPESVLLQTVTGDYVEDDIYYELLDPADIDMI
CRPDQNAVYLVPWAIEPTAQVIHDTYDKQGNPIELSPRNVLKKVLKLYADKGWQPIVAPEMEFYLTKRSDDPDYPLQPPV
GRSGRPEIGRQSFSIEAANEFDPLFEDVYDWCELQELDLDTLIHEDGTAQMEINFRHGDALSLADQILVFKRTMREAALK
HNVAATFMAKPMTGEPGSAMHLHQSIIDIETGKNVFSNDDGTMSQLFLHHIGGLQKLIPELLPLFAPNVNSFRRFLPDTS
APVNVEWGEENRTVGLRVPDAGPQNRRVENRLPGADANPYLAIAASLLCGYIGMVEGINPSAPVVGRGYERRNLRLPLTI
EDALERMENSATIEKYLGKTFITGYVAVKRAEHENFKRVISSWEREYLLFAV
>Mature_451_residues
SNNLDQLTDWLKDHKITEVECMIGDLTGITRGKISPTNKFIAEKGMRLPESVLLQTVTGDYVEDDIYYELLDPADIDMIC
RPDQNAVYLVPWAIEPTAQVIHDTYDKQGNPIELSPRNVLKKVLKLYADKGWQPIVAPEMEFYLTKRSDDPDYPLQPPVG
RSGRPEIGRQSFSIEAANEFDPLFEDVYDWCELQELDLDTLIHEDGTAQMEINFRHGDALSLADQILVFKRTMREAALKH
NVAATFMAKPMTGEPGSAMHLHQSIIDIETGKNVFSNDDGTMSQLFLHHIGGLQKLIPELLPLFAPNVNSFRRFLPDTSA
PVNVEWGEENRTVGLRVPDAGPQNRRVENRLPGADANPYLAIAASLLCGYIGMVEGINPSAPVVGRGYERRNLRLPLTIE
DALERMENSATIEKYLGKTFITGYVAVKRAEHENFKRVISSWEREYLLFAV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=455, Percent_Identity=26.8131868131868, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI87081870, Length=441, Percent_Identity=36.5079365079365, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI1790301, Length=399, Percent_Identity=27.0676691729323, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50988; Mature: 50856

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNLDQLTDWLKDHKITEVECMIGDLTGITRGKISPTNKFIAEKGMRLPESVLLQTVTG
CCCCHHHHHHHHHHCCCCEEEEEEHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCC
DYVEDDIYYELLDPADIDMICRPDQNAVYLVPWAIEPTAQVIHDTYDKQGNPIELSPRNV
CHHCCCCEEEECCCCCCCEEECCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEECHHHH
LKKVLKLYADKGWQPIVAPEMEFYLTKRSDDPDYPLQPPVGRSGRPEIGRQSFSIEAANE
HHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
FDPLFEDVYDWCELQELDLDTLIHEDGTAQMEINFRHGDALSLADQILVFKRTMREAALK
CCHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
HNVAATFMAKPMTGEPGSAMHLHQSIIDIETGKNVFSNDDGTMSQLFLHHIGGLQKLIPE
HHHHHHEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
LLPLFAPNVNSFRRFLPDTSAPVNVEWGEENRTVGLRVPDAGPQNRRVENRLPGADANPY
HHHHHCCCHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCHHHCCCCCCCCHH
LAIAASLLCGYIGMVEGINPSAPVVGRGYERRNLRLPLTIEDALERMENSATIEKYLGKT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCHHHHHHHHHHH
FITGYVAVKRAEHENFKRVISSWEREYLLFAV
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure 
SNNLDQLTDWLKDHKITEVECMIGDLTGITRGKISPTNKFIAEKGMRLPESVLLQTVTG
CCCHHHHHHHHHHCCCCEEEEEEHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCC
DYVEDDIYYELLDPADIDMICRPDQNAVYLVPWAIEPTAQVIHDTYDKQGNPIELSPRNV
CHHCCCCEEEECCCCCCCEEECCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEECHHHH
LKKVLKLYADKGWQPIVAPEMEFYLTKRSDDPDYPLQPPVGRSGRPEIGRQSFSIEAANE
HHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
FDPLFEDVYDWCELQELDLDTLIHEDGTAQMEINFRHGDALSLADQILVFKRTMREAALK
CCHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
HNVAATFMAKPMTGEPGSAMHLHQSIIDIETGKNVFSNDDGTMSQLFLHHIGGLQKLIPE
HHHHHHEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
LLPLFAPNVNSFRRFLPDTSAPVNVEWGEENRTVGLRVPDAGPQNRRVENRLPGADANPY
HHHHHCCCHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCHHHCCCCCCCCHH
LAIAASLLCGYIGMVEGINPSAPVVGRGYERRNLRLPLTIEDALERMENSATIEKYLGKT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCHHHHHHHHHHH
FITGYVAVKRAEHENFKRVISSWEREYLLFAV
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]