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The map label for this gene is potF [H]

Identifier: 77461626

GI number: 77461626

Start: 6068230

End: 6069342

Strand: Reverse

Name: potF [H]

Synonym: Pfl01_5405

Alternate gene names: 77461626

Gene position: 6069342-6068230 (Counterclockwise)

Preceding gene: 77461627

Following gene: 77461625

Centisome position: 94.27

GC content: 56.51

Gene sequence:

>1113_bases
ATGAAGGCATTAGGCAAAAAGCTCGCTGGCAAGACTCTGCTCGCCATGTCCCTGATGGGCGTGATGGCGGGTGCGGTTCA
GGCGGACGACAAGGTGTTGCACGTTTACAACTGGTCCGACTACATCGCGCCGGACACGGTTGCCAATTTCGAAAAAGAGA
CGGGCATCAAGGTCGTCTATGACGTTTTCGACAGCAACGAAACCCTGGAAGCCAAGCTGCTGGCCGGCAAATCCGGTTAC
GACATCGTGGTGCCTTCCAACAACTTCCTGGCCAAGCAGATCAAGGCCGGCGTTTATCAGGAACTGGACAAGTCCAAGCT
GACGAACTGGAAGAACCTCGACGAAGACCTGCTCAAGGCCGTTGGCGACGCCAGCGACCCGGGCAACAAACACGCGTTCC
CGTACATGTGGGGTTCGATCGGCATCGGCTACAACCCGGCCAAGGTCAAGGCTGCCCTGGGCATCGACAAGATCGATTCC
TGGGACATCGTGTTCAAGCCTGAAAACATCGAGAAGCTGAAAAGCTGCGGCGTGAGCTTCCTCGACGCACCGACCGAAAT
GATTCCGGCGGCTTTGCACTACCTGGGCAAACCGACCAACAGCAAAGACAAGGCTGACCTGAAAGCCGCCGAAGACCTGT
TCCTCAAGATTCGTCCTTCGGTGGCTTACTTCCACTCCTCGAAGTACATCTCGGACCTGGCCAACGGCAACATCTGCGTA
GCGGTCGGCTACTCGGGTGACCTGGAGCAATCCAAGACCCGCGCCAAGGAAGCCGGTGACAAGGTCAAACTGGCCTACAC
CATTCCAAAAGAAGGTGCCGGTACTTTCTACGACATGGTCGCCATTCCAAAGGATGCCGAAAACGTCGAAGCCGCCTACA
AGTTCATGAACTACCTGCTCGAGCCGAAAGTGATGGCCGAAATCACCAACGCCGTGCGTTTCCCGAACGGCAACAAGGCG
GCCACCGCCCTGGTGGACAAGGAAATCACCAGCGATCCGAGCATCTACCCTTCGGCTGAAGTGAAGAAGCAGCTCTATGC
GATCAGCGATCTGGACGCAGCCACCCTGCGCCTGATCACTCGCAGCTGGACCAAGATCAAATCCGGTAAATAA

Upstream 100 bases:

>100_bases
AGACCGCCTCGGATCTTGCCAGACTAGCCGCGGTTCCAGTTGTCCGGGTTCGGCCGCTGAACAAAGTGGTTCAAAAAAGA
AAAATTTGGAGCATTAACGC

Downstream 100 bases:

>100_bases
GCCGGTTTGAAGCAGCCACCGCTGGCCGTCGCCTTCACCCGGGCGGTCAGCGGTGCAATTAAATGACAGAAAGTTTTGCT
GGAACGGTTTTTCGAAGGTA

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKALGKKLAGKTLLAMSLMGVMAGAVQADDKVLHVYNWSDYIAPDTVANFEKETGIKVVYDVFDSNETLEAKLLAGKSGY
DIVVPSNNFLAKQIKAGVYQELDKSKLTNWKNLDEDLLKAVGDASDPGNKHAFPYMWGSIGIGYNPAKVKAALGIDKIDS
WDIVFKPENIEKLKSCGVSFLDAPTEMIPAALHYLGKPTNSKDKADLKAAEDLFLKIRPSVAYFHSSKYISDLANGNICV
AVGYSGDLEQSKTRAKEAGDKVKLAYTIPKEGAGTFYDMVAIPKDAENVEAAYKFMNYLLEPKVMAEITNAVRFPNGNKA
ATALVDKEITSDPSIYPSAEVKKQLYAISDLDAATLRLITRSWTKIKSGK

Sequences:

>Translated_370_residues
MKALGKKLAGKTLLAMSLMGVMAGAVQADDKVLHVYNWSDYIAPDTVANFEKETGIKVVYDVFDSNETLEAKLLAGKSGY
DIVVPSNNFLAKQIKAGVYQELDKSKLTNWKNLDEDLLKAVGDASDPGNKHAFPYMWGSIGIGYNPAKVKAALGIDKIDS
WDIVFKPENIEKLKSCGVSFLDAPTEMIPAALHYLGKPTNSKDKADLKAAEDLFLKIRPSVAYFHSSKYISDLANGNICV
AVGYSGDLEQSKTRAKEAGDKVKLAYTIPKEGAGTFYDMVAIPKDAENVEAAYKFMNYLLEPKVMAEITNAVRFPNGNKA
ATALVDKEITSDPSIYPSAEVKKQLYAISDLDAATLRLITRSWTKIKSGK
>Mature_370_residues
MKALGKKLAGKTLLAMSLMGVMAGAVQADDKVLHVYNWSDYIAPDTVANFEKETGIKVVYDVFDSNETLEAKLLAGKSGY
DIVVPSNNFLAKQIKAGVYQELDKSKLTNWKNLDEDLLKAVGDASDPGNKHAFPYMWGSIGIGYNPAKVKAALGIDKIDS
WDIVFKPENIEKLKSCGVSFLDAPTEMIPAALHYLGKPTNSKDKADLKAAEDLFLKIRPSVAYFHSSKYISDLANGNICV
AVGYSGDLEQSKTRAKEAGDKVKLAYTIPKEGAGTFYDMVAIPKDAENVEAAYKFMNYLLEPKVMAEITNAVRFPNGNKA
ATALVDKEITSDPSIYPSAEVKKQLYAISDLDAATLRLITRSWTKIKSGK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=373, Percent_Identity=58.7131367292225, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1787367, Length=350, Percent_Identity=35.1428571428571, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1787711, Length=358, Percent_Identity=27.3743016759777, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40401; Mature: 40401

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALGKKLAGKTLLAMSLMGVMAGAVQADDKVLHVYNWSDYIAPDTVANFEKETGIKVVY
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHCCHHHCCCEEEE
DVFDSNETLEAKLLAGKSGYDIVVPSNNFLAKQIKAGVYQELDKSKLTNWKNLDEDLLKA
EECCCCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
VGDASDPGNKHAFPYMWGSIGIGYNPAKVKAALGIDKIDSWDIVFKPENIEKLKSCGVSF
HCCCCCCCCCCCCCEEECCCCCCCCHHHEEEECCCCCCCCCEEEECCCCHHHHHHCCCCH
LDAPTEMIPAALHYLGKPTNSKDKADLKAAEDLFLKIRPSVAYFHSSKYISDLANGNICV
HCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEEEECCCEEEHCCHHHHHHHCCCCEEE
AVGYSGDLEQSKTRAKEAGDKVKLAYTIPKEGAGTFYDMVAIPKDAENVEAAYKFMNYLL
EECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
EPKVMAEITNAVRFPNGNKAATALVDKEITSDPSIYPSAEVKKQLYAISDLDAATLRLIT
CCHHHHHHHHHHCCCCCCCHHHHEEHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
RSWTKIKSGK
HHHHHHCCCC
>Mature Secondary Structure
MKALGKKLAGKTLLAMSLMGVMAGAVQADDKVLHVYNWSDYIAPDTVANFEKETGIKVVY
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHCCHHHCCCEEEE
DVFDSNETLEAKLLAGKSGYDIVVPSNNFLAKQIKAGVYQELDKSKLTNWKNLDEDLLKA
EECCCCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
VGDASDPGNKHAFPYMWGSIGIGYNPAKVKAALGIDKIDSWDIVFKPENIEKLKSCGVSF
HCCCCCCCCCCCCCEEECCCCCCCCHHHEEEECCCCCCCCCEEEECCCCHHHHHHCCCCH
LDAPTEMIPAALHYLGKPTNSKDKADLKAAEDLFLKIRPSVAYFHSSKYISDLANGNICV
HCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEEEECCCEEEHCCHHHHHHHCCCCEEE
AVGYSGDLEQSKTRAKEAGDKVKLAYTIPKEGAGTFYDMVAIPKDAENVEAAYKFMNYLL
EECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
EPKVMAEITNAVRFPNGNKAATALVDKEITSDPSIYPSAEVKKQLYAISDLDAATLRLIT
CCHHHHHHHHHHCCCCCCCHHHHEEHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
RSWTKIKSGK
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]