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The map label for this gene is potH [H]

Identifier: 77461623

GI number: 77461623

Start: 6064812

End: 6065732

Strand: Reverse

Name: potH [H]

Synonym: Pfl01_5402

Alternate gene names: 77461623

Gene position: 6065732-6064812 (Counterclockwise)

Preceding gene: 77461624

Following gene: 77461622

Centisome position: 94.21

GC content: 58.52

Gene sequence:

>921_bases
ATGAACATGCGCAAATTCAAACGCCGGCTCAATCGAATAATTCCCGGTGGCCGCCAGTTGGTCATCGGGGTTCCGTTCAT
CTGGCTGTTCCTGTTCTTCATGCTGCCGTTCTTCATCGTCCTGAAGATCAGCTTCGCCGAAGCGGACGTGGCCATTCCGC
CGTACACCGAGATCTACACTTACGCCGAGCAGAAGCTGCAACTGCTGCTGAACCTGGGCAACTACGCGATGCTCGGCGAC
GACGAGCTGTACATCGCCGCCTACCTCGGCTCGCTGAAGATGGCATTTTTCAGCACGCTGCTGTGCCTGTTGATCGGTTA
CCCGATGGCCTACGCCATCGCCAGCGCCCGCAAAGAGCTGCAAACGGTGCTGGTGCTGCTGATCATGATGCCGACCTGGA
CCGCGATCCTGATCCGCGTCTATGCGTGGATGGGTATCCTCAGCAACAACGGCCTGCTCAACGGCTTCCTGATGAGCATG
GGTTTGATCGACGAGCCGCTGCAGATTCTCAACACCAACCTCGCGGTGTACATCGGCGTCGTCTACTCGTACCTGCCGTT
CATGATCCTGCCGCTGTACGCCAACCTGGTGAAGCACGACACCAGCCTGCTGGAGGCCGCTTCCGACCTCGGTTCGAGCA
CCTTCAACAGCTTCTGGAAAATCACCGTGCCGCTGTCCAAGAACGGCATCATCGCCGGCTGCATGCTGGTGTTCATCCCG
GTGGTGGGCGAGTTCGTGATCCCGGAACTGCTCGGCGGTCCGGAAACCCTGATGATCGGTAAAGTGCTCTGGCAAGAGTT
CTTCAACAACCGTGACTGGCCGGTGGCGTCCGCCCTGGCGGTGGTGATGCTGGCGATCCTGATCGTGCCGATCATCCTGT
TCAACCGCAGTCAGGCCAAGGAAATGGAGGGTAAAGAATGA

Upstream 100 bases:

>100_bases
AGTCGTTCGTGGCCAACGCCGAGCGCCGTGGTGCGCGTCCGACCTGGGGCGATCAGGTCTACGTCTGGTGGGAAGACGAC
AGCGGCGTGGTACTTCGCTC

Downstream 100 bases:

>100_bases
AGCGCTTCCGTTTCTCCAGCCTGATGCTGGTGCTCGGTCTGTTGTTCATCTACCTGCCGATGCTGATCCTGGTGATCTAC
TCGTTCAACGCCTCAAAACT

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MNMRKFKRRLNRIIPGGRQLVIGVPFIWLFLFFMLPFFIVLKISFAEADVAIPPYTEIYTYAEQKLQLLLNLGNYAMLGD
DELYIAAYLGSLKMAFFSTLLCLLIGYPMAYAIASARKELQTVLVLLIMMPTWTAILIRVYAWMGILSNNGLLNGFLMSM
GLIDEPLQILNTNLAVYIGVVYSYLPFMILPLYANLVKHDTSLLEAASDLGSSTFNSFWKITVPLSKNGIIAGCMLVFIP
VVGEFVIPELLGGPETLMIGKVLWQEFFNNRDWPVASALAVVMLAILIVPIILFNRSQAKEMEGKE

Sequences:

>Translated_306_residues
MNMRKFKRRLNRIIPGGRQLVIGVPFIWLFLFFMLPFFIVLKISFAEADVAIPPYTEIYTYAEQKLQLLLNLGNYAMLGD
DELYIAAYLGSLKMAFFSTLLCLLIGYPMAYAIASARKELQTVLVLLIMMPTWTAILIRVYAWMGILSNNGLLNGFLMSM
GLIDEPLQILNTNLAVYIGVVYSYLPFMILPLYANLVKHDTSLLEAASDLGSSTFNSFWKITVPLSKNGIIAGCMLVFIP
VVGEFVIPELLGGPETLMIGKVLWQEFFNNRDWPVASALAVVMLAILIVPIILFNRSQAKEMEGKE
>Mature_306_residues
MNMRKFKRRLNRIIPGGRQLVIGVPFIWLFLFFMLPFFIVLKISFAEADVAIPPYTEIYTYAEQKLQLLLNLGNYAMLGD
DELYIAAYLGSLKMAFFSTLLCLLIGYPMAYAIASARKELQTVLVLLIMMPTWTAILIRVYAWMGILSNNGLLNGFLMSM
GLIDEPLQILNTNLAVYIGVVYSYLPFMILPLYANLVKHDTSLLEAASDLGSSTFNSFWKITVPLSKNGIIAGCMLVFIP
VVGEFVIPELLGGPETLMIGKVLWQEFFNNRDWPVASALAVVMLAILIVPIILFNRSQAKEMEGKE

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=298, Percent_Identity=60.0671140939597, Blast_Score=347, Evalue=4e-97,
Organism=Escherichia coli, GI1787369, Length=271, Percent_Identity=39.4833948339483, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1787713, Length=243, Percent_Identity=28.8065843621399, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34389; Mature: 34389

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMRKFKRRLNRIIPGGRQLVIGVPFIWLFLFFMLPFFIVLKISFAEADVAIPPYTEIYT
CCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
YAEQKLQLLLNLGNYAMLGDDELYIAAYLGSLKMAFFSTLLCLLIGYPMAYAIASARKEL
HHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTVLVLLIMMPTWTAILIRVYAWMGILSNNGLLNGFLMSMGLIDEPLQILNTNLAVYIGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCHHHHHHH
VYSYLPFMILPLYANLVKHDTSLLEAASDLGSSTFNSFWKITVPLSKNGIIAGCMLVFIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEEEEECCCCHHHHHHHHHHH
VVGEFVIPELLGGPETLMIGKVLWQEFFNNRDWPVASALAVVMLAILIVPIILFNRSQAK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
EMEGKE
CCCCCC
>Mature Secondary Structure
MNMRKFKRRLNRIIPGGRQLVIGVPFIWLFLFFMLPFFIVLKISFAEADVAIPPYTEIYT
CCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
YAEQKLQLLLNLGNYAMLGDDELYIAAYLGSLKMAFFSTLLCLLIGYPMAYAIASARKEL
HHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTVLVLLIMMPTWTAILIRVYAWMGILSNNGLLNGFLMSMGLIDEPLQILNTNLAVYIGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCHHHHHHH
VYSYLPFMILPLYANLVKHDTSLLEAASDLGSSTFNSFWKITVPLSKNGIIAGCMLVFIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEEEEECCCCHHHHHHHHHHH
VVGEFVIPELLGGPETLMIGKVLWQEFFNNRDWPVASALAVVMLAILIVPIILFNRSQAK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
EMEGKE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]