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The map label for this gene is potI [H]

Identifier: 77461622

GI number: 77461622

Start: 6063925

End: 6064815

Strand: Reverse

Name: potI [H]

Synonym: Pfl01_5401

Alternate gene names: 77461622

Gene position: 6064815-6063925 (Counterclockwise)

Preceding gene: 77461623

Following gene: 77461620

Centisome position: 94.2

GC content: 63.86

Gene sequence:

>891_bases
ATGAAGCGCTTCCGTTTCTCCAGCCTGATGCTGGTGCTCGGTCTGTTGTTCATCTACCTGCCGATGCTGATCCTGGTGAT
CTACTCGTTCAACGCCTCAAAACTGGTGACGGTGTGGGGCGGCTGGTCGGTGAAGTGGTACGTCGGCCTGCTCGACAACT
CGCAACTGATGGGCTCGGTGGTGCGCTCGCTGGAAATCGCCTGCTACACCGCGATTGCCGCGGTGGCGCTGGGTACGCTG
GCAGCCTTCGTGCTGACCCGCATCACCCGCTTCAAGGGCCGCACGCTGTTCGGCGGCCTGGTCACCGCGCCGCTGGTCAT
GCCTGAGGTGATTACCGGTCTGTCGCTGTTGCTGCTGTTCGTGGCGATGGCGCAGATGATCGGCTGGCCGCAGGAGCGTG
GCATCGTCACGATCTGGATCGCCCACACCACGTTCTGTGCGGCGTATGTGGCGGTGGTGGTGTCGGCGCGTCTGCGTGAG
CTGGACCTGTCGATCGAAGAAGCGGCGATGGACCTCGGTGCGCGTCCGTGGAAGGTGTTCTTCCTGATCACCATCCCGAT
GATCGCGCCGTCGCTGGCGGCGGGCGGCATGATGTCGTTCGCGCTGTCGCTGGATGACCTGGTATTGGCGAGCTTCGTCT
CCGGCCCGGGTTCAACGACCCTGCCGATGGAAGTGTTCTCGGCGGTGCGTCTGGGCGTGAAGCCTGAGATCAACGCCGTG
GCCAGCCTGATTCTGCTGGCGGTGTCGCTGGTGACCTTCCTGGTCTGGTTCTTCAGCCGCCGTGCCGAAGAAGCCCGCAA
GAAAGCGATTCAGCAGGCCATCGAAGAGGGCGCTGCGGATTCGTGGAAGCAACCGGACGTGCGCCGGGCGCCGACGCCGG
AAGCGGCTTAA

Upstream 100 bases:

>100_bases
GGCCGGTGGCGTCCGCCCTGGCGGTGGTGATGCTGGCGATCCTGATCGTGCCGATCATCCTGTTCAACCGCAGTCAGGCC
AAGGAAATGGAGGGTAAAGA

Downstream 100 bases:

>100_bases
GTCTGCGGATACAAAAAACCTCGCGACCGGTCACGGTCGCGAGGTTTTTTTATGCCGGTCAGGAAACCCAGGGCGACTGG
CGAATCACCTCGACGAAATT

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKRFRFSSLMLVLGLLFIYLPMLILVIYSFNASKLVTVWGGWSVKWYVGLLDNSQLMGSVVRSLEIACYTAIAAVALGTL
AAFVLTRITRFKGRTLFGGLVTAPLVMPEVITGLSLLLLFVAMAQMIGWPQERGIVTIWIAHTTFCAAYVAVVVSARLRE
LDLSIEEAAMDLGARPWKVFFLITIPMIAPSLAAGGMMSFALSLDDLVLASFVSGPGSTTLPMEVFSAVRLGVKPEINAV
ASLILLAVSLVTFLVWFFSRRAEEARKKAIQQAIEEGAADSWKQPDVRRAPTPEAA

Sequences:

>Translated_296_residues
MKRFRFSSLMLVLGLLFIYLPMLILVIYSFNASKLVTVWGGWSVKWYVGLLDNSQLMGSVVRSLEIACYTAIAAVALGTL
AAFVLTRITRFKGRTLFGGLVTAPLVMPEVITGLSLLLLFVAMAQMIGWPQERGIVTIWIAHTTFCAAYVAVVVSARLRE
LDLSIEEAAMDLGARPWKVFFLITIPMIAPSLAAGGMMSFALSLDDLVLASFVSGPGSTTLPMEVFSAVRLGVKPEINAV
ASLILLAVSLVTFLVWFFSRRAEEARKKAIQQAIEEGAADSWKQPDVRRAPTPEAA
>Mature_296_residues
MKRFRFSSLMLVLGLLFIYLPMLILVIYSFNASKLVTVWGGWSVKWYVGLLDNSQLMGSVVRSLEIACYTAIAAVALGTL
AAFVLTRITRFKGRTLFGGLVTAPLVMPEVITGLSLLLLFVAMAQMIGWPQERGIVTIWIAHTTFCAAYVAVVVSARLRE
LDLSIEEAAMDLGARPWKVFFLITIPMIAPSLAAGGMMSFALSLDDLVLASFVSGPGSTTLPMEVFSAVRLGVKPEINAV
ASLILLAVSLVTFLVWFFSRRAEEARKKAIQQAIEEGAADSWKQPDVRRAPTPEAA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=265, Percent_Identity=64.1509433962264, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI1787368, Length=226, Percent_Identity=38.0530973451327, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI1787714, Length=256, Percent_Identity=32.8125, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788764, Length=212, Percent_Identity=29.7169811320755, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32265; Mature: 32265

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFRFSSLMLVLGLLFIYLPMLILVIYSFNASKLVTVWGGWSVKWYVGLLDNSQLMGSV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEECCCHHHHHHH
VRSLEIACYTAIAAVALGTLAAFVLTRITRFKGRTLFGGLVTAPLVMPEVITGLSLLLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VAMAQMIGWPQERGIVTIWIAHTTFCAAYVAVVVSARLRELDLSIEEAAMDLGARPWKVF
HHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEH
FLITIPMIAPSLAAGGMMSFALSLDDLVLASFVSGPGSTTLPMEVFSAVRLGVKPEINAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHH
ASLILLAVSLVTFLVWFFSRRAEEARKKAIQQAIEEGAADSWKQPDVRRAPTPEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRFRFSSLMLVLGLLFIYLPMLILVIYSFNASKLVTVWGGWSVKWYVGLLDNSQLMGSV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEECCCHHHHHHH
VRSLEIACYTAIAAVALGTLAAFVLTRITRFKGRTLFGGLVTAPLVMPEVITGLSLLLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VAMAQMIGWPQERGIVTIWIAHTTFCAAYVAVVVSARLRELDLSIEEAAMDLGARPWKVF
HHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEH
FLITIPMIAPSLAAGGMMSFALSLDDLVLASFVSGPGSTTLPMEVFSAVRLGVKPEINAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHH
ASLILLAVSLVTFLVWFFSRRAEEARKKAIQQAIEEGAADSWKQPDVRRAPTPEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]