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The map label for this gene is gshB [H]

Identifier: 77461530

GI number: 77461530

Start: 5974873

End: 5975823

Strand: Direct

Name: gshB [H]

Synonym: Pfl01_5309

Alternate gene names: 77461530

Gene position: 5974873-5975823 (Clockwise)

Preceding gene: 77461514

Following gene: 77461531

Centisome position: 92.8

GC content: 62.78

Gene sequence:

>951_bases
ATGAGCGTTCGCGTCGGGATTGTCATGGACCCTATCGCCAGCATTTCCTATAAAAAGGATAGCTCGCTGGCCATGCTGCT
GGCGGCGCAAAAGCGCGGCTGGGTACTGTTCTATATGGAACAGAAAGACCTTTATCAGGGTGAAGGCCAGGCACGGGCGC
GGATGAAGCCGCTGAAAGTCTTCGCCAACCCGGAAAAATGGTTCGAACTGGGCACCGAGCAGGATTCGCCGCTGAGCGAC
CTGAACGTGATCCTGATGCGCAAGGATCCGCCGTTCGACATGGAGTTCGTCTACTCCACCTACCTGCTCGAACAGGCCGA
GTCCGCCGGCGTGCTGGTGGTCAACAAGCCGCAGAGCCTGCGCGACTGCAACGAAAAACTGTTCGCCACCCTGTTCCCGC
AGTGCACGCCGCCGACTGTGGTCAGCCGCCGCGCCGACGTGCTGCGTGAGTTCGCCGCCAAACATGGCGACGTGATCCTC
AAGCCGCTGGACGGCATGGGCGGCACCTCGATCTTCCGTCACCGCGCCGGCGATCCGAACCTGTCGGTGATTCTGGAAAC
CCTGACCGCCCTCGGCGCCCAGCAGATCATGGGCCAGGCCTACCTGCCGGCGATCAAGGACGGTGACAAGCGCATCCTGA
TGATCGACGGCGAGCCGGTGGATTACTGCCTGGCGCGGATTCCGGCCCAGGGCGAAACCCGTGGCAACCTCGCGGCCGGC
GGCCGTGGCGAAGCCCGTCCGTTGACCGACAAGGATCGCTGGATCGCCGCTCAGGTCGGCCCGACCTTGCGCGCGAAGGG
CCTGCTGTTCGTCGGCCTGGACGTGATCGGTGAGCACCTGACCGAAATCAACGTCACCAGCCCGACCTGCATCCGCGAAA
TCGACAATGCCTTCGGCACCGACATCGGCGGGATGCTGATGGATGCGATCGAGAAAAAGCTGCAAGCTTGA

Upstream 100 bases:

>100_bases
ATCTATCGACGGGTTTTTCCTTGACCGAAAACCCGCCCGGCGCCACTCTGGCGGCACTTTTTTCATACCGATCCGGTAGC
CAATTTTCGAGGAAAACCCA

Downstream 100 bases:

>100_bases
TGTGCATCACATGCAGCCTGTCGCTTGATGCTTGAAGCTAAAGGCGTGAAACCAACATTGCGTTATCATGCGCGGCCTGT
GAAAAACGCGATGTTGGTTT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSVRVGIVMDPIASISYKKDSSLAMLLAAQKRGWVLFYMEQKDLYQGEGQARARMKPLKVFANPEKWFELGTEQDSPLSD
LNVILMRKDPPFDMEFVYSTYLLEQAESAGVLVVNKPQSLRDCNEKLFATLFPQCTPPTVVSRRADVLREFAAKHGDVIL
KPLDGMGGTSIFRHRAGDPNLSVILETLTALGAQQIMGQAYLPAIKDGDKRILMIDGEPVDYCLARIPAQGETRGNLAAG
GRGEARPLTDKDRWIAAQVGPTLRAKGLLFVGLDVIGEHLTEINVTSPTCIREIDNAFGTDIGGMLMDAIEKKLQA

Sequences:

>Translated_316_residues
MSVRVGIVMDPIASISYKKDSSLAMLLAAQKRGWVLFYMEQKDLYQGEGQARARMKPLKVFANPEKWFELGTEQDSPLSD
LNVILMRKDPPFDMEFVYSTYLLEQAESAGVLVVNKPQSLRDCNEKLFATLFPQCTPPTVVSRRADVLREFAAKHGDVIL
KPLDGMGGTSIFRHRAGDPNLSVILETLTALGAQQIMGQAYLPAIKDGDKRILMIDGEPVDYCLARIPAQGETRGNLAAG
GRGEARPLTDKDRWIAAQVGPTLRAKGLLFVGLDVIGEHLTEINVTSPTCIREIDNAFGTDIGGMLMDAIEKKLQA
>Mature_315_residues
SVRVGIVMDPIASISYKKDSSLAMLLAAQKRGWVLFYMEQKDLYQGEGQARARMKPLKVFANPEKWFELGTEQDSPLSDL
NVILMRKDPPFDMEFVYSTYLLEQAESAGVLVVNKPQSLRDCNEKLFATLFPQCTPPTVVSRRADVLREFAAKHGDVILK
PLDGMGGTSIFRHRAGDPNLSVILETLTALGAQQIMGQAYLPAIKDGDKRILMIDGEPVDYCLARIPAQGETRGNLAAGG
RGEARPLTDKDRWIAAQVGPTLRAKGLLFVGLDVIGEHLTEINVTSPTCIREIDNAFGTDIGGMLMDAIEKKLQA

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=313, Percent_Identity=66.7731629392971, Blast_Score=430, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34753; Mature: 34622

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRVGIVMDPIASISYKKDSSLAMLLAAQKRGWVLFYMEQKDLYQGEGQARARMKPLKV
CCEEEEEEECCHHHCCCCCCCCEEEEEEECCCCEEEEEEECCCHHCCCCCHHHCCCCEEE
FANPEKWFELGTEQDSPLSDLNVILMRKDPPFDMEFVYSTYLLEQAESAGVLVVNKPQSL
ECCHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHH
RDCNEKLFATLFPQCTPPTVVSRRADVLREFAAKHGDVILKPLDGMGGTSIFRHRAGDPN
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCCC
LSVILETLTALGAQQIMGQAYLPAIKDGDKRILMIDGEPVDYCLARIPAQGETRGNLAAG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCC
GRGEARPLTDKDRWIAAQVGPTLRAKGLLFVGLDVIGEHLTEINVTSPTCIREIDNAFGT
CCCCCCCCCCCCCEEEEECCCCHHHCCEEEEEHHHHHHHHHEECCCCCHHHHHHHHHHCC
DIGGMLMDAIEKKLQA
CHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVRVGIVMDPIASISYKKDSSLAMLLAAQKRGWVLFYMEQKDLYQGEGQARARMKPLKV
CEEEEEEECCHHHCCCCCCCCEEEEEEECCCCEEEEEEECCCHHCCCCCHHHCCCCEEE
FANPEKWFELGTEQDSPLSDLNVILMRKDPPFDMEFVYSTYLLEQAESAGVLVVNKPQSL
ECCHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHH
RDCNEKLFATLFPQCTPPTVVSRRADVLREFAAKHGDVILKPLDGMGGTSIFRHRAGDPN
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCCC
LSVILETLTALGAQQIMGQAYLPAIKDGDKRILMIDGEPVDYCLARIPAQGETRGNLAAG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCC
GRGEARPLTDKDRWIAAQVGPTLRAKGLLFVGLDVIGEHLTEINVTSPTCIREIDNAFGT
CCCCCCCCCCCCCEEEEECCCCHHHCCEEEEEHHHHHHHHHEECCCCCHHHHHHHHHHCC
DIGGMLMDAIEKKLQA
CHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]