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The map label for this gene is bioB [H]

Identifier: 77461397

GI number: 77461397

Start: 5821270

End: 5822325

Strand: Reverse

Name: bioB [H]

Synonym: Pfl01_5176

Alternate gene names: 77461397

Gene position: 5822325-5821270 (Counterclockwise)

Preceding gene: 77461403

Following gene: 77461396

Centisome position: 90.43

GC content: 61.27

Gene sequence:

>1056_bases
ATGAGCGCCAGCACCACTGCCACCCTGCGTCACGACTGGACTTTGGCCGAAGTCAAAGCACTGTTCGTTCAGCCATTCAA
CGACTTGCTGTTCCAGGCGCAGACGGTGCACCGCGCGCATTTCGACGCCAACCGCGTCCAGGTTTCCACTCTGCTGTCGA
TCAAGACCGGCGCCTGCCCGGAAGATTGCAAATATTGTCCGCAGTCCGGTCACTACAACACCGGGCTGGAAAAAGAAAAG
CTCATGGAAGTGCAGAAAGTCCTCGAAGAGGCTGCACGCGCCAAGGCCATCGGTTCGACCCGTTTCTGCATGGGCGCGGC
GTGGAAGCACCCGTCGGCCAAGGACATGCCTTATGTGCTGAAGATGGTCGAAGGCGTGAAGGCCATGGGCCTGGAAACCT
GCATGACCCTCGGTCGTCTCGATCAGGATCAGACCGAGGCACTGGCCAAGGCCGGGCTCGACTACTACAACCACAACCTC
GACACCTCGCCGGAGTTCTACGGCAGCATCATCACCACCCGCACCTATGCCGAGCGTCTGCAGACCCTGGCCTATGTGCG
CGATGCGGGGATGAAAATCTGCTCCGGCGGCATCCTCGGTATGGGCGAGTCCCTCGATGACCGCGCGAACCTGCTGATCC
AGTTGGCCAACCTGCCGGAGCACCCGGAATCGGTGCCGATCAACATGCTGGTGAAAGTCGCCGGTACGCCGCTGGAAAAC
GCTGACGACGTCGATCCGTTCGACTTCATCCGCATGCTCGCCGTGGCGCGGATCCTGATGCCGCAATCCCACGTGCGCCT
GTCCGCCGGCCGCGAAGCGATGAACGAGCAGATGCAGGCCCTGGCGTTCTTCGCCGGCGCCAACTCGATCTTCTACGGCG
ACAAACTGCTGACCACCGCCAACCCGCAGGCCGACAAGGACATGCAGTTGTTCGCACGTCTGGGCATTCAGCCGGAAGCA
CGCGAAGAGCATGCTGACGAAGTACATCAGGCTGCCATCGAACAGGCGCTGGTGGAGCAGAAGAGCAGCGAGCAGTTCTA
CAACGCGGCGGTCTGA

Upstream 100 bases:

>100_bases
ACAGCGCATGACTCTTCCTTAAATATGCCGAACATCCGTGTCGTGCCTGTGGGTATTCCGTTTCCCCAGGCGCCAGCCAA
GCATAACCAAGGAAACGCCC

Downstream 100 bases:

>100_bases
TCGCCCGTTCTTCTCAGGCGCTGCCTCGGGCGGTGCCTGCTGATTCATTTGCCACCCGTGAGGCTTGCATGTCTTTCGAT
CTCGCCGCACGCCTTGCTGC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MSASTTATLRHDWTLAEVKALFVQPFNDLLFQAQTVHRAHFDANRVQVSTLLSIKTGACPEDCKYCPQSGHYNTGLEKEK
LMEVQKVLEEAARAKAIGSTRFCMGAAWKHPSAKDMPYVLKMVEGVKAMGLETCMTLGRLDQDQTEALAKAGLDYYNHNL
DTSPEFYGSIITTRTYAERLQTLAYVRDAGMKICSGGILGMGESLDDRANLLIQLANLPEHPESVPINMLVKVAGTPLEN
ADDVDPFDFIRMLAVARILMPQSHVRLSAGREAMNEQMQALAFFAGANSIFYGDKLLTTANPQADKDMQLFARLGIQPEA
REEHADEVHQAAIEQALVEQKSSEQFYNAAV

Sequences:

>Translated_351_residues
MSASTTATLRHDWTLAEVKALFVQPFNDLLFQAQTVHRAHFDANRVQVSTLLSIKTGACPEDCKYCPQSGHYNTGLEKEK
LMEVQKVLEEAARAKAIGSTRFCMGAAWKHPSAKDMPYVLKMVEGVKAMGLETCMTLGRLDQDQTEALAKAGLDYYNHNL
DTSPEFYGSIITTRTYAERLQTLAYVRDAGMKICSGGILGMGESLDDRANLLIQLANLPEHPESVPINMLVKVAGTPLEN
ADDVDPFDFIRMLAVARILMPQSHVRLSAGREAMNEQMQALAFFAGANSIFYGDKLLTTANPQADKDMQLFARLGIQPEA
REEHADEVHQAAIEQALVEQKSSEQFYNAAV
>Mature_350_residues
SASTTATLRHDWTLAEVKALFVQPFNDLLFQAQTVHRAHFDANRVQVSTLLSIKTGACPEDCKYCPQSGHYNTGLEKEKL
MEVQKVLEEAARAKAIGSTRFCMGAAWKHPSAKDMPYVLKMVEGVKAMGLETCMTLGRLDQDQTEALAKAGLDYYNHNLD
TSPEFYGSIITTRTYAERLQTLAYVRDAGMKICSGGILGMGESLDDRANLLIQLANLPEHPESVPINMLVKVAGTPLENA
DDVDPFDFIRMLAVARILMPQSHVRLSAGREAMNEQMQALAFFAGANSIFYGDKLLTTANPQADKDMQLFARLGIQPEAR
EEHADEVHQAAIEQALVEQKSSEQFYNAAV

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=342, Percent_Identity=69.2982456140351, Blast_Score=495, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6321725, Length=317, Percent_Identity=48.2649842271293, Blast_Score=313, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 38806; Mature: 38674

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASTTATLRHDWTLAEVKALFVQPFNDLLFQAQTVHRAHFDANRVQVSTLLSIKTGACP
CCCCCCEEHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEHHHHHEECCCCCC
EDCKYCPQSGHYNTGLEKEKLMEVQKVLEEAARAKAIGSTRFCMGAAWKHPSAKDMPYVL
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHH
KMVEGVKAMGLETCMTLGRLDQDQTEALAKAGLDYYNHNLDTSPEFYGSIITTRTYAERL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHH
QTLAYVRDAGMKICSGGILGMGESLDDRANLLIQLANLPEHPESVPINMLVKVAGTPLEN
HHHHHHHHHCHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHEEECCCCCCC
ADDVDPFDFIRMLAVARILMPQSHVRLSAGREAMNEQMQALAFFAGANSIFYGDKLLTTA
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEECC
NPQADKDMQLFARLGIQPEAREEHADEVHQAAIEQALVEQKSSEQFYNAAV
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SASTTATLRHDWTLAEVKALFVQPFNDLLFQAQTVHRAHFDANRVQVSTLLSIKTGACP
CCCCCEEHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEHHHHHEECCCCCC
EDCKYCPQSGHYNTGLEKEKLMEVQKVLEEAARAKAIGSTRFCMGAAWKHPSAKDMPYVL
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHH
KMVEGVKAMGLETCMTLGRLDQDQTEALAKAGLDYYNHNLDTSPEFYGSIITTRTYAERL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHH
QTLAYVRDAGMKICSGGILGMGESLDDRANLLIQLANLPEHPESVPINMLVKVAGTPLEN
HHHHHHHHHCHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHEEECCCCCCC
ADDVDPFDFIRMLAVARILMPQSHVRLSAGREAMNEQMQALAFFAGANSIFYGDKLLTTA
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEECC
NPQADKDMQLFARLGIQPEAREEHADEVHQAAIEQALVEQKSSEQFYNAAV
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA