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The map label for this gene is bioF [H]

Identifier: 77461396

GI number: 77461396

Start: 5820023

End: 5821201

Strand: Reverse

Name: bioF [H]

Synonym: Pfl01_5175

Alternate gene names: 77461396

Gene position: 5821201-5820023 (Counterclockwise)

Preceding gene: 77461397

Following gene: 77461395

Centisome position: 90.41

GC content: 64.72

Gene sequence:

>1179_bases
ATGTCTTTCGATCTCGCCGCACGCCTTGCTGCCCGTCGTGCCGACAATCTCTATCGCCAGCGACCGCTGCTTGAAAGCCC
GCAAGGGCCGCAAGTGGTGGTCGACGGTCAGCCGCTGCTGGCGTTCTGCAACAACGATTACCTGGGGCTGGCCAATCATC
CGCAAGTGATCGAAGCCTGGCGCGCCGGTGCCTCGAAGTGGGGCGTCGGCGGCGGTGCCTCGCATCTGGTGATCGGCCAC
AGCGGCCCGCATCACGAATTGGAAGAAGCTCTGGCAGACCTCACCGGACGGCCGCGCGCGCTGTTGTTCACCACCGGTTA
CATGGCCAATCTCGGCGCGGTCACGGCGCTGGTCGGGCAGGGCGATACGGTGCTCGAAGACCGGCTCAATCATGCGTCGT
TGCTGGATGCCGGATTGTTGTCCGGCGCGCGCTTCAATCGTTATCTGCACAACGACGCCGACAGCCTCGCCAAGCGTCTG
GAGAAAGCCACCGGCAATACGCTGGTGGTCACTGACGGCGTGTTCAGCATGGACGGCGACCTCGCCGATCTGCCGGCGCT
GGCCCGGGAAACTAAAGCGAAAGGCGCCTGGCTGATGGTCGACGACGCCCACGGTTTCGGCCCGCTGGGGGCAAACGGTG
GCGGGATCGTCGAGCATTTCGGTTTGAGCCAGGAAGACGTGCCGGTGCTGGTCGGCACGCTCGGCAAAGCGTTCGGTACG
GCTGGCGCCTTTGTGGCCGGCAGTGAAGAGCTGATCGAAAGCCTGATCCAGTTCGCCCGTCCGTACATCTACACCACCAG
CCAGCCGCCAGCGCTGGCCTGCGCAACCTTGAAGAGTCTGGAGCTGCTGCGCAGCGAACACTGGCGTCGCGAGCATCTGC
AGACGCTGATCCGCCAGTTCCGCCACGGCGCCGAACAGATCGGTCTGGAACTTATGGACAGCTTCACGCCGATTCAGCCG
ATCCTGATCGGCGATGCCGGTCGCGCCGTGCGTCTGTCGCAGATGCTGCGCGAGCGTGGACTGATGGTCACCGCGATCCG
CCCGCCGACCGTGCCTGCCGGCAGCGCGCGGTTGCGGGTGACGCTGACCGCCGCTCACAGCGAGGCACAGGTGCAGCTAT
TGTTGGAAGGACTGGCCGATTGCTTTGCGCAACTCAAGTCGGAGCCAAGCCATGCGTGA

Upstream 100 bases:

>100_bases
GCAGCGAGCAGTTCTACAACGCGGCGGTCTGATCGCCCGTTCTTCTCAGGCGCTGCCTCGGGCGGTGCCTGCTGATTCAT
TTGCCACCCGTGAGGCTTGC

Downstream 100 bases:

>100_bases
TCGCCTGATTCTGCTACCGGGCTGGGGCCTCGGCGTTTCGCCGCTGGAGCCGCTGGCCGCTGCGTTGCGCGGACTCGACG
AACACCTGCAGGTCGACATC

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSFDLAARLAARRADNLYRQRPLLESPQGPQVVVDGQPLLAFCNNDYLGLANHPQVIEAWRAGASKWGVGGGASHLVIGH
SGPHHELEEALADLTGRPRALLFTTGYMANLGAVTALVGQGDTVLEDRLNHASLLDAGLLSGARFNRYLHNDADSLAKRL
EKATGNTLVVTDGVFSMDGDLADLPALARETKAKGAWLMVDDAHGFGPLGANGGGIVEHFGLSQEDVPVLVGTLGKAFGT
AGAFVAGSEELIESLIQFARPYIYTTSQPPALACATLKSLELLRSEHWRREHLQTLIRQFRHGAEQIGLELMDSFTPIQP
ILIGDAGRAVRLSQMLRERGLMVTAIRPPTVPAGSARLRVTLTAAHSEAQVQLLLEGLADCFAQLKSEPSHA

Sequences:

>Translated_392_residues
MSFDLAARLAARRADNLYRQRPLLESPQGPQVVVDGQPLLAFCNNDYLGLANHPQVIEAWRAGASKWGVGGGASHLVIGH
SGPHHELEEALADLTGRPRALLFTTGYMANLGAVTALVGQGDTVLEDRLNHASLLDAGLLSGARFNRYLHNDADSLAKRL
EKATGNTLVVTDGVFSMDGDLADLPALARETKAKGAWLMVDDAHGFGPLGANGGGIVEHFGLSQEDVPVLVGTLGKAFGT
AGAFVAGSEELIESLIQFARPYIYTTSQPPALACATLKSLELLRSEHWRREHLQTLIRQFRHGAEQIGLELMDSFTPIQP
ILIGDAGRAVRLSQMLRERGLMVTAIRPPTVPAGSARLRVTLTAAHSEAQVQLLLEGLADCFAQLKSEPSHA
>Mature_391_residues
SFDLAARLAARRADNLYRQRPLLESPQGPQVVVDGQPLLAFCNNDYLGLANHPQVIEAWRAGASKWGVGGGASHLVIGHS
GPHHELEEALADLTGRPRALLFTTGYMANLGAVTALVGQGDTVLEDRLNHASLLDAGLLSGARFNRYLHNDADSLAKRLE
KATGNTLVVTDGVFSMDGDLADLPALARETKAKGAWLMVDDAHGFGPLGANGGGIVEHFGLSQEDVPVLVGTLGKAFGTA
GAFVAGSEELIESLIQFARPYIYTTSQPPALACATLKSLELLRSEHWRREHLQTLIRQFRHGAEQIGLELMDSFTPIQPI
LIGDAGRAVRLSQMLRERGLMVTAIRPPTVPAGSARLRVTLTAAHSEAQVQLLLEGLADCFAQLKSEPSHA

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=381, Percent_Identity=35.4330708661417, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI284448556, Length=407, Percent_Identity=33.1695331695332, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI83977442, Length=353, Percent_Identity=34.2776203966006, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI83977444, Length=353, Percent_Identity=34.2776203966006, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI83977440, Length=353, Percent_Identity=34.2776203966006, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI4758668, Length=342, Percent_Identity=31.2865497076023, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI119220554, Length=354, Percent_Identity=29.0960451977401, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI4502025, Length=354, Percent_Identity=33.0508474576271, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI40316939, Length=354, Percent_Identity=33.0508474576271, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI5454084, Length=260, Percent_Identity=24.2307692307692, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1786993, Length=353, Percent_Identity=47.5920679886686, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1790046, Length=387, Percent_Identity=36.4341085271318, Blast_Score=244, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI71994529, Length=382, Percent_Identity=34.2931937172775, Blast_Score=219, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI32566772, Length=352, Percent_Identity=28.125, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17560914, Length=316, Percent_Identity=28.4810126582279, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17560912, Length=316, Percent_Identity=28.4810126582279, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI71982617, Length=322, Percent_Identity=23.6024844720497, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71982625, Length=322, Percent_Identity=23.6024844720497, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320438, Length=414, Percent_Identity=28.9855072463768, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6320267, Length=362, Percent_Identity=27.0718232044199, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6323954, Length=267, Percent_Identity=27.3408239700375, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24662918, Length=386, Percent_Identity=32.9015544041451, Blast_Score=228, Evalue=8e-60,
Organism=Drosophila melanogaster, GI17137420, Length=349, Percent_Identity=31.8051575931232, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI17136286, Length=353, Percent_Identity=28.0453257790368, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24653280, Length=284, Percent_Identity=24.6478873239437, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24653276, Length=284, Percent_Identity=24.6478873239437, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24653278, Length=284, Percent_Identity=24.6478873239437, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022834
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.47 [H]

Molecular weight: Translated: 41971; Mature: 41840

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDLAARLAARRADNLYRQRPLLESPQGPQVVVDGQPLLAFCNNDYLGLANHPQVIEAW
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCEECCCCCHHHHHHH
RAGASKWGVGGGASHLVIGHSGPHHELEEALADLTGRPRALLFTTGYMANLGAVTALVGQ
HCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCC
GDTVLEDRLNHASLLDAGLLSGARFNRYLHNDADSLAKRLEKATGNTLVVTDGVFSMDGD
CCHHHHHHCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCEECCCC
LADLPALARETKAKGAWLMVDDAHGFGPLGANGGGIVEHFGLSQEDVPVLVGTLGKAFGT
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCC
AGAFVAGSEELIESLIQFARPYIYTTSQPPALACATLKSLELLRSEHWRREHLQTLIRQF
CCCEECCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHGAEQIGLELMDSFTPIQPILIGDAGRAVRLSQMLRERGLMVTAIRPPTVPAGSARLRV
HCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEE
TLTAAHSEAQVQLLLEGLADCFAQLKSEPSHA
EEEECCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SFDLAARLAARRADNLYRQRPLLESPQGPQVVVDGQPLLAFCNNDYLGLANHPQVIEAW
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCEECCCCCHHHHHHH
RAGASKWGVGGGASHLVIGHSGPHHELEEALADLTGRPRALLFTTGYMANLGAVTALVGQ
HCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCC
GDTVLEDRLNHASLLDAGLLSGARFNRYLHNDADSLAKRLEKATGNTLVVTDGVFSMDGD
CCHHHHHHCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCEECCCC
LADLPALARETKAKGAWLMVDDAHGFGPLGANGGGIVEHFGLSQEDVPVLVGTLGKAFGT
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCC
AGAFVAGSEELIESLIQFARPYIYTTSQPPALACATLKSLELLRSEHWRREHLQTLIRQF
CCCEECCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHGAEQIGLELMDSFTPIQPILIGDAGRAVRLSQMLRERGLMVTAIRPPTVPAGSARLRV
HCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEE
TLTAAHSEAQVQLLLEGLADCFAQLKSEPSHA
EEEECCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA