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The map label for this gene is cca

Identifier: 77461363

GI number: 77461363

Start: 5779071

End: 5780300

Strand: Direct

Name: cca

Synonym: Pfl01_5142

Alternate gene names: 77461363

Gene position: 5779071-5780300 (Clockwise)

Preceding gene: 77461362

Following gene: 77461366

Centisome position: 89.76

GC content: 60.57

Gene sequence:

>1230_bases
ATGCAGATTTACAAGGTCGGCGGTGCGGTACGTGATCGCCTGCTGGGCAAACCGGTCACCGATATCGATTGGGTGGTGGT
CGGGGCGACTACAGAAGAAATGCTCGCCAAGGGATTTCGCCCGGTCGGCGCGGATTTTCCGGTGTTTCTTCACCCGAAAA
GTGGCGAGGAGTACGCCCTCGCCCGCACCGAGCGCAAGAGCGGTCGCGGTTATGGTGGTTTCACCTTTCACGCCAGCCCC
GAGGTCACGCTTGAAGAAGACCTGATCCGCCGCGACCTTACGATCAACGCCATGGCCGAGGATGATCAGCAAAACCTGAC
CGATCCCTACCACGGTCAGCGCGATCTCGAAGAGCGGATTCTGCGCCACGTTTCCCCCGCGTTTGCCGAAGATCCGCTCA
GGGTCCTGCGTGTTGCACGCTTTGCCGCTCGCTACGCGGAGCTTGGTTTCAAGGTAGCGCCCGAGACCCTGGAGCTGATG
CGTCAGCTCAGCGAATCGGGTGAACTGGAAGCCCTTACGGCCGAACGCAGCTGGAAAGAGATTTCCCGCGCACTGATGGA
AGATCAGCCCCAGGTGTTCATTCAGGTGTTGCGCGATTGCGGCGCCTTGAAGGTGCTGATGCCGGAGGTCGATGCCTTGT
TCGGCGTGCCGCAACCGGAAGCCCATCACCCGGAAATCGACAGCGGCCTTCACACCCTGAGCGTGCTTGAGCAGTCAGCC
TTGCACAAACAACCGCTGACCGTGCGCTGGGCCTGCCTGCTGCACGACCTCGGCAAAGGTCTTACTCCAGAGGAGGAATG
GCCACGGCACATCGCCCATGAGCACAAAGGCCTGAAGTTGATCAAAGCAGTCAACGAACGCTTCAAGGCACCGAAGGACT
GTCAGGAACTGGCCTTGCTGGTCGGCCAATATCACACCCACGGGCATCGCGCCCTGGAGCTGAAGGCTTCGACCTTGCTC
GAGTTGCTGCAAAGCTTTGACGTTTACCGTCGGCCACAGCGTTTCGAAGAGTTCATCGTCGCGTGCGAGATGGATGCCCG
AGGCCGCAAAGGGCTCGAGCAACGCAGTTATCCACAGGCGGATTACCTGCGCGGCGCGGCCAATGTGGCACGCGGCGTGG
CGGTGCAGCCGTTGCTGGAAAAGGGATTCAAAGGCCCGGAACTGGGCGAGGCACTCAAGCGCGAACGGCTCAAGGCGTTG
AAGGTTTACAAGGAATCGGCGGCGTCCTGA

Upstream 100 bases:

>100_bases
TTACGGTCGACTCGACCTGGCCGTCATTCATCTTTGACTCCGTTGAGACCTCCAGAAGTCCGGCGCAAGGGTTATCCTGT
CGGGCCAACGGAGGTTTTTT

Downstream 100 bases:

>100_bases
AAGCACCTGATCCGCCCTCGCGAGGGCGGATCAAACTGTTCAAGACAGATCTGAAGGCGTGAGCTGAATACCGCGCCACT
CGAATCCGACCGGTGCCAGC

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MQIYKVGGAVRDRLLGKPVTDIDWVVVGATTEEMLAKGFRPVGADFPVFLHPKSGEEYALARTERKSGRGYGGFTFHASP
EVTLEEDLIRRDLTINAMAEDDQQNLTDPYHGQRDLEERILRHVSPAFAEDPLRVLRVARFAARYAELGFKVAPETLELM
RQLSESGELEALTAERSWKEISRALMEDQPQVFIQVLRDCGALKVLMPEVDALFGVPQPEAHHPEIDSGLHTLSVLEQSA
LHKQPLTVRWACLLHDLGKGLTPEEEWPRHIAHEHKGLKLIKAVNERFKAPKDCQELALLVGQYHTHGHRALELKASTLL
ELLQSFDVYRRPQRFEEFIVACEMDARGRKGLEQRSYPQADYLRGAANVARGVAVQPLLEKGFKGPELGEALKRERLKAL
KVYKESAAS

Sequences:

>Translated_409_residues
MQIYKVGGAVRDRLLGKPVTDIDWVVVGATTEEMLAKGFRPVGADFPVFLHPKSGEEYALARTERKSGRGYGGFTFHASP
EVTLEEDLIRRDLTINAMAEDDQQNLTDPYHGQRDLEERILRHVSPAFAEDPLRVLRVARFAARYAELGFKVAPETLELM
RQLSESGELEALTAERSWKEISRALMEDQPQVFIQVLRDCGALKVLMPEVDALFGVPQPEAHHPEIDSGLHTLSVLEQSA
LHKQPLTVRWACLLHDLGKGLTPEEEWPRHIAHEHKGLKLIKAVNERFKAPKDCQELALLVGQYHTHGHRALELKASTLL
ELLQSFDVYRRPQRFEEFIVACEMDARGRKGLEQRSYPQADYLRGAANVARGVAVQPLLEKGFKGPELGEALKRERLKAL
KVYKESAAS
>Mature_409_residues
MQIYKVGGAVRDRLLGKPVTDIDWVVVGATTEEMLAKGFRPVGADFPVFLHPKSGEEYALARTERKSGRGYGGFTFHASP
EVTLEEDLIRRDLTINAMAEDDQQNLTDPYHGQRDLEERILRHVSPAFAEDPLRVLRVARFAARYAELGFKVAPETLELM
RQLSESGELEALTAERSWKEISRALMEDQPQVFIQVLRDCGALKVLMPEVDALFGVPQPEAHHPEIDSGLHTLSVLEQSA
LHKQPLTVRWACLLHDLGKGLTPEEEWPRHIAHEHKGLKLIKAVNERFKAPKDCQELALLVGQYHTHGHRALELKASTLL
ELLQSFDVYRRPQRFEEFIVACEMDARGRKGLEQRSYPQADYLRGAANVARGVAVQPLLEKGFKGPELGEALKRERLKAL
KVYKESAAS

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=207, Percent_Identity=31.4009661835749, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789436, Length=405, Percent_Identity=57.7777777777778, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI87081691, Length=178, Percent_Identity=34.2696629213483, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_PSEPF (Q3K5S5)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_350870.1
- ProteinModelPortal:   Q3K5S5
- SMR:   Q3K5S5
- STRING:   Q3K5S5
- GeneID:   3717273
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_5142
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   RWACLLH
- ProtClustDB:   PRK10885
- BioCyc:   PFLU205922:PFL_5142-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 46100; Mature: 46100

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIYKVGGAVRDRLLGKPVTDIDWVVVGATTEEMLAKGFRPVGADFPVFLHPKSGEEYAL
CEEEECCHHHHHHHCCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEEECCCCCCHHH
ARTERKSGRGYGGFTFHASPEVTLEEDLIRRDLTINAMAEDDQQNLTDPYHGQRDLEERI
HHHHHHCCCCCCCEEEECCCCCCHHHHHHHHCCEEEECCCCCHHCCCCCCCCHHHHHHHH
LRHVSPAFAEDPLRVLRVARFAARYAELGFKVAPETLELMRQLSESGELEALTAERSWKE
HHHCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHH
ISRALMEDQPQVFIQVLRDCGALKVLMPEVDALFGVPQPEAHHPEIDSGLHTLSVLEQSA
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
LHKQPLTVRWACLLHDLGKGLTPEEEWPRHIAHEHKGLKLIKAVNERFKAPKDCQELALL
HHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHH
VGQYHTHGHRALELKASTLLELLQSFDVYRRPQRFEEFIVACEMDARGRKGLEQRSYPQA
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHCCCCCH
DYLRGAANVARGVAVQPLLEKGFKGPELGEALKRERLKALKVYKESAAS
HHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQIYKVGGAVRDRLLGKPVTDIDWVVVGATTEEMLAKGFRPVGADFPVFLHPKSGEEYAL
CEEEECCHHHHHHHCCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEEECCCCCCHHH
ARTERKSGRGYGGFTFHASPEVTLEEDLIRRDLTINAMAEDDQQNLTDPYHGQRDLEERI
HHHHHHCCCCCCCEEEECCCCCCHHHHHHHHCCEEEECCCCCHHCCCCCCCCHHHHHHHH
LRHVSPAFAEDPLRVLRVARFAARYAELGFKVAPETLELMRQLSESGELEALTAERSWKE
HHHCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHH
ISRALMEDQPQVFIQVLRDCGALKVLMPEVDALFGVPQPEAHHPEIDSGLHTLSVLEQSA
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
LHKQPLTVRWACLLHDLGKGLTPEEEWPRHIAHEHKGLKLIKAVNERFKAPKDCQELALL
HHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHH
VGQYHTHGHRALELKASTLLELLQSFDVYRRPQRFEEFIVACEMDARGRKGLEQRSYPQA
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHCCCCCH
DYLRGAANVARGVAVQPLLEKGFKGPELGEALKRERLKALKVYKESAAS
HHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA