Click here to switch to the map view.

The map label for this gene is potH [H]

Identifier: 77461344

GI number: 77461344

Start: 5755599

End: 5756846

Strand: Reverse

Name: potH [H]

Synonym: Pfl01_5123

Alternate gene names: 77461344

Gene position: 5756846-5755599 (Counterclockwise)

Preceding gene: 77461345

Following gene: 77461343

Centisome position: 89.41

GC content: 61.86

Gene sequence:

>1248_bases
ATGGCCATCGCCGTTCCCCTGAACGAGGGCAACAGCCCCACCTTGAAGCAGCGGCTCAAGCACGCCGAGCGGGTCAACCG
CTGGAAAGCCCAGGCGCTGATCGCGCCGCTGGTGCTGTTTCTGTTGCTGGTGTTCCTGGTGCCGATCGTGGCGCTGCTCT
ATAAAAGCGTCGGCAACCCGGAAGTGGTCAGCGGCATGCCGCGCACCGTGACTGCGATTGCCGCGTGGGACGGCCGAGGC
CTGCCCGCAGAACCGGTCTATAAAGCCGCCAGCGAAGACCTCGCCGAAGCGCGCAAAAACCAGACCCTGGGCGATCTGTC
CAAGCGCCTGAACATGGAGTTGGCCGGCTATCGCAGCCTGCTGACCAAAACCGCCCGCTCGCTGCCATTCGCCAGCGAAC
CGGCCTCTTATAAAGAAGCGCTGGAAGGCCTCGACGAGCGTTGGGGCGATCCGGCCTACTGGCAAGCGGTGAAGCGCAAC
ACCAGCAGCATCACCCCGTTCTATCTGCTGGCGGCGGTGGATCACCGCATCGACGACCTCGGCGAAATTGCCCCGGCGAC
CCCGGATCAGGCGATCTACCTCGACATCTTCGCCCGGACCTTCTGGATGGGCCTGATCATCACCGTGATCTGCCTGGTGC
TGGCGTATCCGCTGGCTTACCTGCTGGCCAACCTGCCATCGCGTCAAAGCAACCTGCTGATGATTCTGGTGCTGCTGCCG
TTCTGGACTTCGATCCTGGTACGGGTCGCCGCGTGGATCGTGTTGCTGCAATCCGGTGGTTTGATCAACAGCGCGCTGAT
GGCGATGGGCATCATCGATAAACCGCTGGAACTGGTGTTCAACCGCACCGGGGTCTACATCTCGATGGTGCACATCCTGC
TGCCGTTCATGATCCTGCCGATCTACAGCGTGATGAAAGGCATCTCGCCGACCTACATGCGTGCTGCGATTTCCCTGGGT
TGTCATCCGTTCGCCAGTTTCTGGCGGGTGTACTTCCCGCAGACCTATGCCGGTGTCGGTGCCGGTTGCCTGTTGGTGTT
CATCCTCGCCATTGGCTACTACATCACCCCGGCGCTGCTGGGCAGCCCGAACGATCAGATGGTCAGCTACTTCGTCGCGT
TCTACACCAACACCAGCATCAACTGGGGCATGGCGACCGCACTCGGCGGGCTGTTGCTGCTGGCGACCGTGGTGCTTTAT
CTGATCTACAGCTGGCTGGTGGGCGCCAGTCGCCTGCGCCTGAGCTAA

Upstream 100 bases:

>100_bases
GGAGCAGCGCTTCAATTCCTGGGCTGCGAAGTAAGGCTGATGCATACGCGGCGTCACCCCCAGGGTGACGCCGTCATTTA
AAAGATTTGCGGAGTACGTC

Downstream 100 bases:

>100_bases
GGGGAATTTGAAATGCTGAGTCCTTATATGTCGCCCGTCGAGCGGGTGTGGTTCTACAGCTTGCGGATCCTCTGCGGCTT
GATCCTGTTGTTTCTGATCC

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MAIAVPLNEGNSPTLKQRLKHAERVNRWKAQALIAPLVLFLLLVFLVPIVALLYKSVGNPEVVSGMPRTVTAIAAWDGRG
LPAEPVYKAASEDLAEARKNQTLGDLSKRLNMELAGYRSLLTKTARSLPFASEPASYKEALEGLDERWGDPAYWQAVKRN
TSSITPFYLLAAVDHRIDDLGEIAPATPDQAIYLDIFARTFWMGLIITVICLVLAYPLAYLLANLPSRQSNLLMILVLLP
FWTSILVRVAAWIVLLQSGGLINSALMAMGIIDKPLELVFNRTGVYISMVHILLPFMILPIYSVMKGISPTYMRAAISLG
CHPFASFWRVYFPQTYAGVGAGCLLVFILAIGYYITPALLGSPNDQMVSYFVAFYTNTSINWGMATALGGLLLLATVVLY
LIYSWLVGASRLRLS

Sequences:

>Translated_415_residues
MAIAVPLNEGNSPTLKQRLKHAERVNRWKAQALIAPLVLFLLLVFLVPIVALLYKSVGNPEVVSGMPRTVTAIAAWDGRG
LPAEPVYKAASEDLAEARKNQTLGDLSKRLNMELAGYRSLLTKTARSLPFASEPASYKEALEGLDERWGDPAYWQAVKRN
TSSITPFYLLAAVDHRIDDLGEIAPATPDQAIYLDIFARTFWMGLIITVICLVLAYPLAYLLANLPSRQSNLLMILVLLP
FWTSILVRVAAWIVLLQSGGLINSALMAMGIIDKPLELVFNRTGVYISMVHILLPFMILPIYSVMKGISPTYMRAAISLG
CHPFASFWRVYFPQTYAGVGAGCLLVFILAIGYYITPALLGSPNDQMVSYFVAFYTNTSINWGMATALGGLLLLATVVLY
LIYSWLVGASRLRLS
>Mature_414_residues
AIAVPLNEGNSPTLKQRLKHAERVNRWKAQALIAPLVLFLLLVFLVPIVALLYKSVGNPEVVSGMPRTVTAIAAWDGRGL
PAEPVYKAASEDLAEARKNQTLGDLSKRLNMELAGYRSLLTKTARSLPFASEPASYKEALEGLDERWGDPAYWQAVKRNT
SSITPFYLLAAVDHRIDDLGEIAPATPDQAIYLDIFARTFWMGLIITVICLVLAYPLAYLLANLPSRQSNLLMILVLLPF
WTSILVRVAAWIVLLQSGGLINSALMAMGIIDKPLELVFNRTGVYISMVHILLPFMILPIYSVMKGISPTYMRAAISLGC
HPFASFWRVYFPQTYAGVGAGCLLVFILAIGYYITPALLGSPNDQMVSYFVAFYTNTSINWGMATALGGLLLLATVVLYL
IYSWLVGASRLRLS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=212, Percent_Identity=37.2641509433962, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1787369, Length=221, Percent_Identity=33.9366515837104, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787713, Length=184, Percent_Identity=30.9782608695652, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 45723; Mature: 45591

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIAVPLNEGNSPTLKQRLKHAERVNRWKAQALIAPLVLFLLLVFLVPIVALLYKSVGNP
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EVVSGMPRTVTAIAAWDGRGLPAEPVYKAASEDLAEARKNQTLGDLSKRLNMELAGYRSL
HHHCCCCHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHH
LTKTARSLPFASEPASYKEALEGLDERWGDPAYWQAVKRNTSSITPFYLLAAVDHRIDDL
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
GEIAPATPDQAIYLDIFARTFWMGLIITVICLVLAYPLAYLLANLPSRQSNLLMILVLLP
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
FWTSILVRVAAWIVLLQSGGLINSALMAMGIIDKPLELVFNRTGVYISMVHILLPFMILP
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
IYSVMKGISPTYMRAAISLGCHPFASFWRVYFPQTYAGVGAGCLLVFILAIGYYITPALL
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHHHHHHHC
GSPNDQMVSYFVAFYTNTSINWGMATALGGLLLLATVVLYLIYSWLVGASRLRLS
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
AIAVPLNEGNSPTLKQRLKHAERVNRWKAQALIAPLVLFLLLVFLVPIVALLYKSVGNP
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EVVSGMPRTVTAIAAWDGRGLPAEPVYKAASEDLAEARKNQTLGDLSKRLNMELAGYRSL
HHHCCCCHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHH
LTKTARSLPFASEPASYKEALEGLDERWGDPAYWQAVKRNTSSITPFYLLAAVDHRIDDL
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
GEIAPATPDQAIYLDIFARTFWMGLIITVICLVLAYPLAYLLANLPSRQSNLLMILVLLP
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
FWTSILVRVAAWIVLLQSGGLINSALMAMGIIDKPLELVFNRTGVYISMVHILLPFMILP
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
IYSVMKGISPTYMRAAISLGCHPFASFWRVYFPQTYAGVGAGCLLVFILAIGYYITPALL
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHHHHHHHC
GSPNDQMVSYFVAFYTNTSINWGMATALGGLLLLATVVLYLIYSWLVGASRLRLS
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]