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The map label for this gene is potC [H]

Identifier: 77461343

GI number: 77461343

Start: 5754762

End: 5755586

Strand: Reverse

Name: potC [H]

Synonym: Pfl01_5122

Alternate gene names: 77461343

Gene position: 5755586-5754762 (Counterclockwise)

Preceding gene: 77461344

Following gene: 77461342

Centisome position: 89.39

GC content: 61.82

Gene sequence:

>825_bases
ATGCTGAGTCCTTATATGTCGCCCGTCGAGCGGGTGTGGTTCTACAGCTTGCGGATCCTCTGCGGCTTGATCCTGTTGTT
TCTGATCCTGCCGGTGCTGGTGATCGTGCCGCTGTCGTTCAACTCGGGCAGTTTCCTGGTGTACCCGCTGCAAGGTTTCT
CGCTGCACTGGTATCAGGACTTCTTCGCTTCGGCGGAATGGATGCGCTCGCTGAAGAACAGCATCATCGTCGCCCCGGCC
GCGACGGTACTGGCAATGGTCTTCGGCACGCTGGCGGCCATCGGCCTGACCCGTGGCGACTTCCCCGGCAAGCCGCTGGT
GATGGCGCTGGTGATTTCGCCGATGGTGGTGCCAGTGGTGATCATCGGTGTGGCCAGTTACCTGTTCTTCGCGCCGCTGG
GCCTGGGCAACAGTTTCTTTTCGCTGATCGTGGTGCACGCGGTGCTGGGTGTGCCGTTCGTGATCATCACGGTGTCGGCG
ACCCTGCAGGGGTTCAATCACAACCTGGTGCGGGCGGCCGCCAGCCTTGGCGCCTCGCCACTGACCACTTTCCGTCGGGT
GACCTTGCCGCTGATTGCGCCGGGGGTGATTTCCGGTGCGCTGTTTGCCTTTGCAACCTCGTTCGATGAAGTGGTGGTGA
CGCTGTTTCTTGCAGGTCCCGAGCAGGCGACGTTGCCTCGGCAGATGTTCAGCGGGATTCGCGAGAACCTGAGCCCGACG
ATTGCGGCGGCTGCGACTCTGCTGATTGCCTTCTCGGTGATTCTGTTGCTGACGCTGGAGTGGTTGCGTGGGCGGAGTGA
GAAGCTGCGCACCGCTCAGGTCTGA

Upstream 100 bases:

>100_bases
ACTCGGCGGGCTGTTGCTGCTGGCGACCGTGGTGCTTTATCTGATCTACAGCTGGCTGGTGGGCGCCAGTCGCCTGCGCC
TGAGCTAAGGGGAATTTGAA

Downstream 100 bases:

>100_bases
CAACTGCACAATCCGGGTTGTCTGCCATTCATGCGCTGAATGACAGACAACCCGATTTCCCCAGCTACGCTTGTGCTCAG
CTCCCACATCTATAAGAGGC

Product: binding-protein dependent transport system inner membrane protein

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MLSPYMSPVERVWFYSLRILCGLILLFLILPVLVIVPLSFNSGSFLVYPLQGFSLHWYQDFFASAEWMRSLKNSIIVAPA
ATVLAMVFGTLAAIGLTRGDFPGKPLVMALVISPMVVPVVIIGVASYLFFAPLGLGNSFFSLIVVHAVLGVPFVIITVSA
TLQGFNHNLVRAAASLGASPLTTFRRVTLPLIAPGVISGALFAFATSFDEVVVTLFLAGPEQATLPRQMFSGIRENLSPT
IAAAATLLIAFSVILLLTLEWLRGRSEKLRTAQV

Sequences:

>Translated_274_residues
MLSPYMSPVERVWFYSLRILCGLILLFLILPVLVIVPLSFNSGSFLVYPLQGFSLHWYQDFFASAEWMRSLKNSIIVAPA
ATVLAMVFGTLAAIGLTRGDFPGKPLVMALVISPMVVPVVIIGVASYLFFAPLGLGNSFFSLIVVHAVLGVPFVIITVSA
TLQGFNHNLVRAAASLGASPLTTFRRVTLPLIAPGVISGALFAFATSFDEVVVTLFLAGPEQATLPRQMFSGIRENLSPT
IAAAATLLIAFSVILLLTLEWLRGRSEKLRTAQV
>Mature_274_residues
MLSPYMSPVERVWFYSLRILCGLILLFLILPVLVIVPLSFNSGSFLVYPLQGFSLHWYQDFFASAEWMRSLKNSIIVAPA
ATVLAMVFGTLAAIGLTRGDFPGKPLVMALVISPMVVPVVIIGVASYLFFAPLGLGNSFFSLIVVHAVLGVPFVIITVSA
TLQGFNHNLVRAAASLGASPLTTFRRVTLPLIAPGVISGALFAFATSFDEVVVTLFLAGPEQATLPRQMFSGIRENLSPT
IAAAATLLIAFSVILLLTLEWLRGRSEKLRTAQV

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=236, Percent_Identity=32.2033898305085, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1787081, Length=255, Percent_Identity=33.7254901960784, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787714, Length=246, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788764, Length=185, Percent_Identity=28.6486486486486, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1786980, Length=217, Percent_Identity=29.9539170506912, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787080, Length=118, Percent_Identity=28.8135593220339, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29557; Mature: 29557

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]