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The map label for this gene is gph [C]

Identifier: 77461340

GI number: 77461340

Start: 5751901

End: 5752719

Strand: Reverse

Name: gph [C]

Synonym: Pfl01_5119

Alternate gene names: 77461340

Gene position: 5752719-5751901 (Counterclockwise)

Preceding gene: 77461341

Following gene: 77461339

Centisome position: 89.35

GC content: 63.0

Gene sequence:

>819_bases
ATGAGCGGTTTTGAGCAGCTGTTCCCGGGGCAACTGCCGCGGTTGGTGATGTTCGATCTGGATGGCACGCTGATCGATTC
GGTACCCGACCTGGCGGCGGCTGTGGATAACATGCTGCTCTCCCTCGGGCGCAAGCCTGCCGGTATCGAGTCGGTGCGCG
AGTGGGTCGGCAACGGTGCGCCGGTGCTGGTGCGCCGCGCCTTGGCCGGTGGCATCGACCATTCTTCCGTGGATGACGTC
GAAGCCGAGCACGCGCTGGAAGTTTTCATGGAGGCCTACGGCGCCAGCCATGAGCTGACCGTGGTCTATCCCGGCGTGCG
CGACACCCTGAAATGGCTGCACAAGCAGGGCGTGGCCATGGCGCTGATCACCAACAAGCCGGAGCGCTTCGTCGCGCCGC
TGCTGGATCAGATGAAGATCGGCCGCTACTTCAAGTGGATCATCGGCGGCGATACCCTGCCGCAGAAGAAGCCCGATCCG
GCGGCGCTGTTCTTCGTCATGAAGATGTCCGGGATTCCAGCCTCGCAATCGCTGTTCGTCGGCGATTCGCGCAGCGACGT
GCTGGCGGCGAAAGCGGCGGGGGTCAAATGTGTCGGGCTGAGTTATGGCTACAACCATGGCCGACCGATTGCCGAGGAAT
CCCCGACGCTGGTGATCGACGATCTGCGCAAGCTAATTCCCGGTTGCCTGGATACGGCCGCTGAGATAACGTTGCCCGAC
GCTGCTCAATCCCCTTCTGGAAACGCCATCGTGGTGGTCACCCGCAAACTCTGGATGAAAGTCATCAAGGCCCTGGCCCG
CTGGCGTTGGCGCGCCTGA

Upstream 100 bases:

>100_bases
CCGGTGCCGACACCTTTGTCGCCGGTTCGGCGATCTTCAATGCGCCGAACTATCAGGAAGTCATCGACAAGATGCGTTCC
GAACTGGCGCTGGCTCGCCC

Downstream 100 bases:

>100_bases
CTTGTTCCTGGCCGGCTTGCCGGCGCGTTTGCACACCTGACTGATTTGCACCTCACACCACGAGGCACCCTATGATCCGC
GAAGAATTCCTGCGTCTGGC

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSGFEQLFPGQLPRLVMFDLDGTLIDSVPDLAAAVDNMLLSLGRKPAGIESVREWVGNGAPVLVRRALAGGIDHSSVDDV
EAEHALEVFMEAYGASHELTVVYPGVRDTLKWLHKQGVAMALITNKPERFVAPLLDQMKIGRYFKWIIGGDTLPQKKPDP
AALFFVMKMSGIPASQSLFVGDSRSDVLAAKAAGVKCVGLSYGYNHGRPIAEESPTLVIDDLRKLIPGCLDTAAEITLPD
AAQSPSGNAIVVVTRKLWMKVIKALARWRWRA

Sequences:

>Translated_272_residues
MSGFEQLFPGQLPRLVMFDLDGTLIDSVPDLAAAVDNMLLSLGRKPAGIESVREWVGNGAPVLVRRALAGGIDHSSVDDV
EAEHALEVFMEAYGASHELTVVYPGVRDTLKWLHKQGVAMALITNKPERFVAPLLDQMKIGRYFKWIIGGDTLPQKKPDP
AALFFVMKMSGIPASQSLFVGDSRSDVLAAKAAGVKCVGLSYGYNHGRPIAEESPTLVIDDLRKLIPGCLDTAAEITLPD
AAQSPSGNAIVVVTRKLWMKVIKALARWRWRA
>Mature_271_residues
SGFEQLFPGQLPRLVMFDLDGTLIDSVPDLAAAVDNMLLSLGRKPAGIESVREWVGNGAPVLVRRALAGGIDHSSVDDVE
AEHALEVFMEAYGASHELTVVYPGVRDTLKWLHKQGVAMALITNKPERFVAPLLDQMKIGRYFKWIIGGDTLPQKKPDPA
ALFFVMKMSGIPASQSLFVGDSRSDVLAAKAAGVKCVGLSYGYNHGRPIAEESPTLVIDDLRKLIPGCLDTAAEITLPDA
AQSPSGNAIVVVTRKLWMKVIKALARWRWRA

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family

Homologues:

Organism=Escherichia coli, GI1789787, Length=243, Percent_Identity=43.6213991769547, Blast_Score=178, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPH_PSEPF (Q3K5U8)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_350847.1
- ProteinModelPortal:   Q3K5U8
- SMR:   Q3K5U8
- STRING:   Q3K5U8
- GeneID:   3717037
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_5119
- eggNOG:   COG0546
- HOGENOM:   HBG742904
- OMA:   DSSNDAQ
- ProtClustDB:   PRK13223
- BioCyc:   PFLU205922:PFL_5119-MONOMER
- HAMAP:   MF_00495
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198
- Gene3D:   G3DSA:3.40.50.1000
- Gene3D:   G3DSA:1.10.150.240
- PRINTS:   PR00413
- TIGRFAMs:   TIGR01549
- TIGRFAMs:   TIGR01509
- TIGRFAMs:   TIGR01449

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: =3.1.3.18

Molecular weight: Translated: 29559; Mature: 29428

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: ACT_SITE 19-19

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFEQLFPGQLPRLVMFDLDGTLIDSVPDLAAAVDNMLLSLGRKPAGIESVREWVGNGA
CCCHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
PVLVRRALAGGIDHSSVDDVEAEHALEVFMEAYGASHELTVVYPGVRDTLKWLHKQGVAM
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCEE
ALITNKPERFVAPLLDQMKIGRYFKWIIGGDTLPQKKPDPAALFFVMKMSGIPASQSLFV
EEECCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHEEEEEHHCCCCCCCEEEE
GDSRSDVLAAKAAGVKCVGLSYGYNHGRPIAEESPTLVIDDLRKLIPGCLDTAAEITLPD
CCCCCHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCHHHEECCC
AAQSPSGNAIVVVTRKLWMKVIKALARWRWRA
CCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGFEQLFPGQLPRLVMFDLDGTLIDSVPDLAAAVDNMLLSLGRKPAGIESVREWVGNGA
CCHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
PVLVRRALAGGIDHSSVDDVEAEHALEVFMEAYGASHELTVVYPGVRDTLKWLHKQGVAM
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCEE
ALITNKPERFVAPLLDQMKIGRYFKWIIGGDTLPQKKPDPAALFFVMKMSGIPASQSLFV
EEECCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHEEEEEHHCCCCCCCEEEE
GDSRSDVLAAKAAGVKCVGLSYGYNHGRPIAEESPTLVIDDLRKLIPGCLDTAAEITLPD
CCCCCHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCHHHEECCC
AAQSPSGNAIVVVTRKLWMKVIKALARWRWRA
CCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA