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The map label for this gene is tyrS [H]

Identifier: 77461319

GI number: 77461319

Start: 5732113

End: 5733312

Strand: Reverse

Name: tyrS [H]

Synonym: Pfl01_5098

Alternate gene names: 77461319

Gene position: 5733312-5732113 (Counterclockwise)

Preceding gene: 77461322

Following gene: 77461317

Centisome position: 89.05

GC content: 54.42

Gene sequence:

>1200_bases
ATGAAGTCGGTTGAAGAGCAGCTAGCGCTGATTAAACGTGGTGCGGAAGAACTGTTGGTCGAGTCCGAGCTGATCGAAAA
GCTCAAGCGTGGCCAGCCGCTGCGAATCAAGGCCGGCTTCGATCCAACTGCGCCGGATCTGCACCTTGGTCACACCGTGC
TTATTAATAAGCTGCGCCAGTTCCAGGAACTGGGTCATCAGGTGATCTTCCTTATAGGTGACTTCACCGGAATGATCGGT
GATCCGAGCGGCAAGAGCGCCACTCGCCCTCCGCTGACGCGTGAGCAGGTTCTGGAAAACGCCGAGACTTACAAGACTCA
GGTGTTCAAGATTCTTGATCCGGCGAAAACCGAAGTTGCGTTCAACTCCACCTGGATGGATCAGATGGGGCCTGCGGACT
TTATTCGTCTGACCTCGCAGTACACCGTTGCTCGTATGCTCGAGCGTGACGATTTCGATAAGCGCTATACCACCAATCAG
CCAATCGCCATTCACGAATTCCTCTATCCGCTGGTTCAGGGTTATGACTCTGTAGCGTTGCGCGCTGACGTTGAGCTGGG
TGGTACTGATCAGAAGTTCAATCTGCTGATGGGGCGTGAGCTGCAGCGTGGTTATGGCCAGGAAGCCCAGTGCATTCTGA
CGATGCCGCTGCTTGAGGGTTTGGATGGCGTGAAGAAGATGTCCAAATCGCTGGGCAACTACGTCGGTATCCAGGAAGCG
CCAGGTGTCATGTATAGCAAGCTGGTTTCGATTCCCGATGCGTTGATGTGGCGCTACTTCGAGTTGCTCAGCTTCCGTTC
GATGGATGAGATAAATGCTTTCCGTGCTGATGTTGAAGCGGGTGCTAACCCGCGTGATATCAAGATCAAGCTGGCCGAAG
AGATTGTTGCGCGCTTCCATGGTGAAGAGGCCGCGGCCAATGCTCACCGTGGTGCGGGCAATCGTATGAAGGATGGCGAG
TTGCCGGATGATCTGCCAGAGGTTGAGCTGACTGCTGCCGAGGATATGCCGATTGCTGCTGTCCTTAATAAGGCAGGTCT
GGTGAAGAACTCGGCAGCTGCTCGTGATCTGCTGGCCTCCGGTGGTGTGCGTGTAGATGGTGAGGTGGTTGATCGCTCCT
TTATATACGTACTGGGCGCGACTCACGTTTGTCAGGCCGGCAAGAAGGCTTTTGCACGGATTACGCTGAAATCTGAGTAA

Upstream 100 bases:

>100_bases
TCTGTCCGTGGATTGGCGTCCGTGCGCCGGGCAAACCTTGTATTTGGTGCGCGATCTTGTATGGTTGGTTCCCTTTGAAT
CTGAGCCTTGCGGGTCTGTT

Downstream 100 bases:

>100_bases
AGATGAAATAAGGGGTTGACGGCGAATTCCAGAGGTCTATAATTCGCCCCACTTCCGGCGCAGTCGAAACGGAAAACTCC
TTGAGATTCAATGAGTTAAG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MKSVEEQLALIKRGAEELLVESELIEKLKRGQPLRIKAGFDPTAPDLHLGHTVLINKLRQFQELGHQVIFLIGDFTGMIG
DPSGKSATRPPLTREQVLENAETYKTQVFKILDPAKTEVAFNSTWMDQMGPADFIRLTSQYTVARMLERDDFDKRYTTNQ
PIAIHEFLYPLVQGYDSVALRADVELGGTDQKFNLLMGRELQRGYGQEAQCILTMPLLEGLDGVKKMSKSLGNYVGIQEA
PGVMYSKLVSIPDALMWRYFELLSFRSMDEINAFRADVEAGANPRDIKIKLAEEIVARFHGEEAAANAHRGAGNRMKDGE
LPDDLPEVELTAAEDMPIAAVLNKAGLVKNSAAARDLLASGGVRVDGEVVDRSFIYVLGATHVCQAGKKAFARITLKSE

Sequences:

>Translated_399_residues
MKSVEEQLALIKRGAEELLVESELIEKLKRGQPLRIKAGFDPTAPDLHLGHTVLINKLRQFQELGHQVIFLIGDFTGMIG
DPSGKSATRPPLTREQVLENAETYKTQVFKILDPAKTEVAFNSTWMDQMGPADFIRLTSQYTVARMLERDDFDKRYTTNQ
PIAIHEFLYPLVQGYDSVALRADVELGGTDQKFNLLMGRELQRGYGQEAQCILTMPLLEGLDGVKKMSKSLGNYVGIQEA
PGVMYSKLVSIPDALMWRYFELLSFRSMDEINAFRADVEAGANPRDIKIKLAEEIVARFHGEEAAANAHRGAGNRMKDGE
LPDDLPEVELTAAEDMPIAAVLNKAGLVKNSAAARDLLASGGVRVDGEVVDRSFIYVLGATHVCQAGKKAFARITLKSE
>Mature_399_residues
MKSVEEQLALIKRGAEELLVESELIEKLKRGQPLRIKAGFDPTAPDLHLGHTVLINKLRQFQELGHQVIFLIGDFTGMIG
DPSGKSATRPPLTREQVLENAETYKTQVFKILDPAKTEVAFNSTWMDQMGPADFIRLTSQYTVARMLERDDFDKRYTTNQ
PIAIHEFLYPLVQGYDSVALRADVELGGTDQKFNLLMGRELQRGYGQEAQCILTMPLLEGLDGVKKMSKSLGNYVGIQEA
PGVMYSKLVSIPDALMWRYFELLSFRSMDEINAFRADVEAGANPRDIKIKLAEEIVARFHGEEAAANAHRGAGNRMKDGE
LPDDLPEVELTAAEDMPIAAVLNKAGLVKNSAAARDLLASGGVRVDGEVVDRSFIYVLGATHVCQAGKKAFARITLKSE

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=400, Percent_Identity=27.75, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=31.8396226415094, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25145802, Length=295, Percent_Identity=27.4576271186441, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6325160, Length=317, Percent_Identity=25.8675078864353, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI20130383, Length=397, Percent_Identity=25.9445843828715, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44183; Mature: 44183

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVEEQLALIKRGAEELLVESELIEKLKRGQPLRIKAGFDPTAPDLHLGHTVLINKLRQ
CCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
FQELGHQVIFLIGDFTGMIGDPSGKSATRPPLTREQVLENAETYKTQVFKILDPAKTEVA
HHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEE
FNSTWMDQMGPADFIRLTSQYTVARMLERDDFDKRYTTNQPIAIHEFLYPLVQGYDSVAL
ECCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
RADVELGGTDQKFNLLMGRELQRGYGQEAQCILTMPLLEGLDGVKKMSKSLGNYVGIQEA
EEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHCHHHHHHHHHHHHCCCCCCCC
PGVMYSKLVSIPDALMWRYFELLSFRSMDEINAFRADVEAGANPRDIKIKLAEEIVARFH
CCHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHC
GEEAAANAHRGAGNRMKDGELPDDLPEVELTAAEDMPIAAVLNKAGLVKNSAAARDLLAS
CCHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHC
GGVRVDGEVVDRSFIYVLGATHVCQAGKKAFARITLKSE
CCCEECHHHHCCHHHEEHHHHHHHHHHHHHEEEEEEECC
>Mature Secondary Structure
MKSVEEQLALIKRGAEELLVESELIEKLKRGQPLRIKAGFDPTAPDLHLGHTVLINKLRQ
CCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
FQELGHQVIFLIGDFTGMIGDPSGKSATRPPLTREQVLENAETYKTQVFKILDPAKTEVA
HHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEE
FNSTWMDQMGPADFIRLTSQYTVARMLERDDFDKRYTTNQPIAIHEFLYPLVQGYDSVAL
ECCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEE
RADVELGGTDQKFNLLMGRELQRGYGQEAQCILTMPLLEGLDGVKKMSKSLGNYVGIQEA
EEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHCHHHHHHHHHHHHCCCCCCCC
PGVMYSKLVSIPDALMWRYFELLSFRSMDEINAFRADVEAGANPRDIKIKLAEEIVARFH
CCHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHC
GEEAAANAHRGAGNRMKDGELPDDLPEVELTAAEDMPIAAVLNKAGLVKNSAAARDLLAS
CCHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHC
GGVRVDGEVVDRSFIYVLGATHVCQAGKKAFARITLKSE
CCCEECHHHHCCHHHEEHHHHHHHHHHHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA