BacMap

Definition	Pseudomonas fluorescens Pf0-1 chromosome, complete genome.
Accession	NC_007492
Length	6,438,405

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The map label for this gene is dkgB [H]

Identifier: 77461133

GI number: 77461133

Start: 5535731

End: 5536564

Strand: Direct

Name: dkgB [H]

Synonym: Pfl01_4912

Alternate gene names: 77461133

Gene position: 5535731-5536564 (Clockwise)

Preceding gene: 77461132

Following gene: 77461134

Centisome position: 85.98

GC content: 61.15

Gene sequence:

>834_bases
ATGCAGCACATCGTTAACAGACAAGGACTGAACCTGCCAAAACTCGGCCTCGGCACCTGGCCGATGCTTGGCGACGAATG
CACCCGCGCCGTGGAGCAAGCGCTGGAACTCGGTTACCGCCACATCGACACGGCAGCGGCCTACAACAACGAAGACGCAG
TCGGACAGGCGTTGGCAAATACGCCGACACCCCGCGAGCAGATCCACGTCACCACCAAGGTCTGGTGGGACCAGCTGCAA
CCGGACGCCATGCGTCACTCGCTGGATCGCAGCCTCAAGGCGTTGCGCAGCGAGTACGTCGATCTGTTCATGATCCATTG
GCCGACCACTGACTGGGACCTGCCGCACACCCTTGAAACCCTCGCGTCGTTCAAGGAGCAAGGTCTGGCTCGCAACATCG
GTGTGGCGAACTTTCCGCTGCCGCTGCTGCGCAAAGTCGTCGAGGAATACGGCATCCCTCTGTCAGCCATCCAGATCGAG
TACCACGTCCTGCTCGGACAAAACGCCCTGCTCGACTACGCCCGTCAACACGATCTGGCGCTGACGGCCTACACGCCACT
GGCGCGCAACAAGGTCTCGGAGATTCCACAGATTCAGCAGATCGCCGAAAAACATGGCGTGCTGCCGACTCAAGTGGCGC
TGAAGTGGCTGCTGGATCAGGACAACGTCGCGGCGATTCCCAAGGCCAGCAGCAAGACCAATCAACTGGCCAACCTGGCG
TCACTGCAGGTCGAACTGGATGACGACGACCGAGCGTTGATCGCCGCGTTGTCCAAACGCGAACGTCAGGTCAGCCCGGA
CTTCGCGCCGGTGTGGGATGCCTTCGACCGCTGA

Upstream 100 bases:

>100_bases
TACCCGGCGGGCGGGATCAACCTCGGACCGGCGCTGACGTTCGGTTACATCGCCGCCCGGCACATGGCGGGCGTCACGGC
TTTCGAACAGGAGATCGACC

Downstream 100 bases:

>100_bases
TACCTGACCCGGCGAAAGAAATCCGCCGGCGAATTGAATTCCCGGCGCTCGCGCTCGTCAATAACAGGCCCGACCTCGTC
ACCCCGAGGCCGGGCCTTTG

Product: aldo/keto reductase

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MQHIVNRQGLNLPKLGLGTWPMLGDECTRAVEQALELGYRHIDTAAAYNNEDAVGQALANTPTPREQIHVTTKVWWDQLQ
PDAMRHSLDRSLKALRSEYVDLFMIHWPTTDWDLPHTLETLASFKEQGLARNIGVANFPLPLLRKVVEEYGIPLSAIQIE
YHVLLGQNALLDYARQHDLALTAYTPLARNKVSEIPQIQQIAEKHGVLPTQVALKWLLDQDNVAAIPKASSKTNQLANLA
SLQVELDDDDRALIAALSKRERQVSPDFAPVWDAFDR

Sequences:

>Translated_277_residues
MQHIVNRQGLNLPKLGLGTWPMLGDECTRAVEQALELGYRHIDTAAAYNNEDAVGQALANTPTPREQIHVTTKVWWDQLQ
PDAMRHSLDRSLKALRSEYVDLFMIHWPTTDWDLPHTLETLASFKEQGLARNIGVANFPLPLLRKVVEEYGIPLSAIQIE
YHVLLGQNALLDYARQHDLALTAYTPLARNKVSEIPQIQQIAEKHGVLPTQVALKWLLDQDNVAAIPKASSKTNQLANLA
SLQVELDDDDRALIAALSKRERQVSPDFAPVWDAFDR
>Mature_277_residues
MQHIVNRQGLNLPKLGLGTWPMLGDECTRAVEQALELGYRHIDTAAAYNNEDAVGQALANTPTPREQIHVTTKVWWDQLQ
PDAMRHSLDRSLKALRSEYVDLFMIHWPTTDWDLPHTLETLASFKEQGLARNIGVANFPLPLLRKVVEEYGIPLSAIQIE
YHVLLGQNALLDYARQHDLALTAYTPLARNKVSEIPQIQQIAEKHGVLPTQVALKWLLDQDNVAAIPKASSKTNQLANLA
SLQVELDDDDRALIAALSKRERQVSPDFAPVWDAFDR

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=292, Percent_Identity=30.4794520547945, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI5174391, Length=292, Percent_Identity=30.4794520547945, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI310109922, Length=279, Percent_Identity=31.1827956989247, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI310109920, Length=298, Percent_Identity=30.5369127516779, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI45446745, Length=298, Percent_Identity=30.2013422818792, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI4503285, Length=298, Percent_Identity=30.2013422818792, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI24497585, Length=306, Percent_Identity=30.3921568627451, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI5453543, Length=298, Percent_Identity=30.2013422818792, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI223468663, Length=287, Percent_Identity=27.8745644599303, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI93277124, Length=300, Percent_Identity=27.6666666666667, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI24497583, Length=290, Percent_Identity=30.3448275862069, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI5174695, Length=297, Percent_Identity=29.2929292929293, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI300116273, Length=262, Percent_Identity=30.1526717557252, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI291291012, Length=290, Percent_Identity=26.8965517241379, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI4502049, Length=289, Percent_Identity=28.0276816608997, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI300116271, Length=275, Percent_Identity=27.2727272727273, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI207028673, Length=109, Percent_Identity=40.3669724770642, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI310109926, Length=109, Percent_Identity=41.2844036697248, Blast_Score=85, Evalue=6e-17,
Organism=Escherichia coli, GI1786400, Length=268, Percent_Identity=39.1791044776119, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI87082198, Length=264, Percent_Identity=35.9848484848485, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1788081, Length=273, Percent_Identity=30.7692307692308, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788070, Length=317, Percent_Identity=27.4447949526814, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789375, Length=296, Percent_Identity=27.027027027027, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87081735, Length=274, Percent_Identity=25.9124087591241, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787674, Length=251, Percent_Identity=23.5059760956175, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17566692, Length=290, Percent_Identity=28.6206896551724, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17537075, Length=301, Percent_Identity=26.578073089701, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17537077, Length=299, Percent_Identity=27.4247491638796, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17561298, Length=273, Percent_Identity=27.8388278388278, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17550248, Length=292, Percent_Identity=28.7671232876712, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71998625, Length=262, Percent_Identity=28.6259541984733, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17552492, Length=263, Percent_Identity=28.1368821292776, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17561300, Length=256, Percent_Identity=31.640625, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17538386, Length=271, Percent_Identity=29.1512915129151, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17564128, Length=300, Percent_Identity=27.3333333333333, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17537079, Length=298, Percent_Identity=25.503355704698, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17562292, Length=273, Percent_Identity=27.1062271062271, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324694, Length=301, Percent_Identity=33.2225913621262, Blast_Score=149, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6320576, Length=284, Percent_Identity=30.9859154929577, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6321896, Length=279, Percent_Identity=27.9569892473118, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6319625, Length=264, Percent_Identity=32.5757575757576, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6322556, Length=258, Percent_Identity=27.5193798449612, Blast_Score=96, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6320079, Length=245, Percent_Identity=28.1632653061224, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24644950, Length=303, Percent_Identity=30.6930693069307, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI21356425, Length=294, Percent_Identity=30.2721088435374, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI45553081, Length=262, Percent_Identity=32.824427480916, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI221378297, Length=323, Percent_Identity=28.7925696594427, Blast_Score=140, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24657054, Length=286, Percent_Identity=29.3706293706294, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI20129731, Length=283, Percent_Identity=31.8021201413428, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24663317, Length=290, Percent_Identity=31.0344827586207, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24662789, Length=291, Percent_Identity=29.2096219931272, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI281366140, Length=241, Percent_Identity=30.7053941908714, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24662781, Length=272, Percent_Identity=30.8823529411765, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24662785, Length=276, Percent_Identity=31.1594202898551, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24646155, Length=300, Percent_Identity=24, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24646159, Length=163, Percent_Identity=31.2883435582822, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 31173; Mature: 31173

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHIVNRQGLNLPKLGLGTWPMLGDECTRAVEQALELGYRHIDTAAAYNNEDAVGQALAN
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
TPTPREQIHVTTKVWWDQLQPDAMRHSLDRSLKALRSEYVDLFMIHWPTTDWDLPHTLET
CCCCHHHEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHH
LASFKEQGLARNIGVANFPLPLLRKVVEEYGIPLSAIQIEYHVLLGQNALLDYARQHDLA
HHHHHHCCHHHCCCCCCCCHHHHHHHHHHHCCCEEHEEEEEEEEECCHHHHHHHHHCCEE
LTAYTPLARNKVSEIPQIQQIAEKHGVLPTQVALKWLLDQDNVAAIPKASSKTNQLANLA
EEECCCHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHH
SLQVELDDDDRALIAALSKRERQVSPDFAPVWDAFDR
HEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHCCC
>Mature Secondary Structure
MQHIVNRQGLNLPKLGLGTWPMLGDECTRAVEQALELGYRHIDTAAAYNNEDAVGQALAN
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
TPTPREQIHVTTKVWWDQLQPDAMRHSLDRSLKALRSEYVDLFMIHWPTTDWDLPHTLET
CCCCHHHEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHH
LASFKEQGLARNIGVANFPLPLLRKVVEEYGIPLSAIQIEYHVLLGQNALLDYARQHDLA
HHHHHHCCHHHCCCCCCCCHHHHHHHHHHHCCCEEHEEEEEEEEECCHHHHHHHHHCCEE
LTAYTPLARNKVSEIPQIQQIAEKHGVLPTQVALKWLLDQDNVAAIPKASSKTNQLANLA
EEECCCHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHH
SLQVELDDDDRALIAALSKRERQVSPDFAPVWDAFDR
HEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796; 11108008 [H]