Click here to switch to the map view.

The map label for this gene is kstD [H]

Identifier: 77461132

GI number: 77461132

Start: 5534011

End: 5535747

Strand: Direct

Name: kstD [H]

Synonym: Pfl01_4911

Alternate gene names: 77461132

Gene position: 5534011-5535747 (Clockwise)

Preceding gene: 77461128

Following gene: 77461133

Centisome position: 85.95

GC content: 65.28

Gene sequence:

>1737_bases
ATGTCTGCCCTTCCCGATATCGACTGCGACGTTCTAGTGGTCGGCTCCGGCGCCGCCGGTTTGTCGGCCGCCGTCACCGC
CGCCTGGCATGGCTTGAAGGTCATCGTGGTGGAAAAGGATCCGGTGTTCGGCGGCGCCACCGCGTGGTCCGGCGGCTGGG
CGTGGGTGCCGTGCAATCCGCTGGCCTGGCGCGCCGGGATCGTCGAAGACGTGGAATTGCCGCGCACCTATCTGCGTCAC
GAACTCGGCGAGCACTACGACCCGGCCATGATCGACGCCTTCCTTGAGGCCGGCCCGCGCATGGTGGCGTTCTTCGAACA
ACACACCGCCCTGCAATTCGCCGACGGCAACGCCATCGCCGACATCCACGGTGAAACACCGGGCGCCGGCACCGGCGGAC
GCTCGGTGATTGCCGCTCCCTACGACGGACGAAAGGTCGGACGCCTGCTCAAGCGTCTTCGCACAACCATGCGCGAAACT
TCATTCATGGGCATGCCGATCATGGCCGGTGCGGACCTGTCGGCGTTTCTCAATCTGACTCGCTCGTTGTCGGCAGCCTG
GCACGTGACCCGGCGTTTCACTCGCCACCTGCTGGACCTGGCACTGCACGGCCGGGCGATGCAACTGGTCAACGGCGTGG
CGCTGGTGGCGCGGCTGGGGAAATCCGCCGAAGACCTCGGCGTTCTGCTGTGGGAGTCGGCACCGGTGACCGAACTGCTG
CGCGACGAATCACGCATCACCGGCGCTCGCGTGAACACCACAAAAGGCCCGCTCAATGTCCATGCCCGCAAAGCCGTGGT
GCTCGCCGCTGGTGGGTTCGCCAATGACATCGAGCGGCGCAGAGCGTTGTTCCCTCGTACGCCCACCGGTCATGAACATT
GGGCGCTGCCACCCTTGGGAGTTAATGGCGACGGCCTGCGACTGGGTGAAACCGTCGGGGGCCGGGTCAACACCGATGTG
GCCTCGCCAGTCGCCTGGGCCCCGGTCTCGCAGGTGCCACACTCCGACGGCAGCATCGGCCATTTCCCACACATCATCGA
GCGCGGCAAACCCGGCGTCATCGGCGTGCTGCGCAACGGCCAGCGCTTCGTCAACGAAGCCAACGGCTATTACGACTACG
TCAGCGCCATGGTCGCCGCCGCCCCCGAGGGTGAAGAGGTCGCGTCCTGGCTGATCTGCACCCGCGCGTTCCAGCGGCGT
TACGGGCTCGGCATGTCTCGTCCGTTTCCGGTTCCGGTGTCAGGTTTTATCCGCAGCGGCTATCTGAAAACCGGCAATAC
CCTTGAGGAATTGGCCACCGCATGCGGCATCGATCCGATCGGTTTACGCGCCACTCTGCATGACTACAACCACCATGCCC
GGCACGGCAAAGACCCGCTGTTCGGTCGCGGCTCGACCCCGTACAACCGCAAACAGGGCGATCCGGCACAACAGCCCAAC
CCGTGCGTGGCCCCTATAGAACAAGGCCCGTTCTACGCGGTGAAGGTGCAGCCTGGTTGCTTCGGCACCTTCGCCGGGCT
CAAGGTCAACCCGCATGCACAAGTGCTCGACACCGAAGACCGGCCTATCCCCGGGCTGTACGCCGCCGGTGGCGACATGG
CCAGCATCATGGGCGGGCATTACCCGGCGGGCGGGATCAACCTCGGACCGGCGCTGACGTTCGGTTACATCGCCGCCCGG
CACATGGCGGGCGTCACGGCTTTCGAACAGGAGATCGACCATGCAGCACATCGTTAA

Upstream 100 bases:

>100_bases
ACCGCCCACTTTGCGCGTCAGTCAGTTGATCTGCTTCCTCCTCACTGGTTATTTTCTGGAACATGATTCTAAAAATAATT
GATTGCTGGAGCCCTTGCTG

Downstream 100 bases:

>100_bases
CAGACAAGGACTGAACCTGCCAAAACTCGGCCTCGGCACCTGGCCGATGCTTGGCGACGAATGCACCCGCGCCGTGGAGC
AAGCGCTGGAACTCGGTTAC

Product: putative FAD-binding dehydrogenase

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MSALPDIDCDVLVVGSGAAGLSAAVTAAWHGLKVIVVEKDPVFGGATAWSGGWAWVPCNPLAWRAGIVEDVELPRTYLRH
ELGEHYDPAMIDAFLEAGPRMVAFFEQHTALQFADGNAIADIHGETPGAGTGGRSVIAAPYDGRKVGRLLKRLRTTMRET
SFMGMPIMAGADLSAFLNLTRSLSAAWHVTRRFTRHLLDLALHGRAMQLVNGVALVARLGKSAEDLGVLLWESAPVTELL
RDESRITGARVNTTKGPLNVHARKAVVLAAGGFANDIERRRALFPRTPTGHEHWALPPLGVNGDGLRLGETVGGRVNTDV
ASPVAWAPVSQVPHSDGSIGHFPHIIERGKPGVIGVLRNGQRFVNEANGYYDYVSAMVAAAPEGEEVASWLICTRAFQRR
YGLGMSRPFPVPVSGFIRSGYLKTGNTLEELATACGIDPIGLRATLHDYNHHARHGKDPLFGRGSTPYNRKQGDPAQQPN
PCVAPIEQGPFYAVKVQPGCFGTFAGLKVNPHAQVLDTEDRPIPGLYAAGGDMASIMGGHYPAGGINLGPALTFGYIAAR
HMAGVTAFEQEIDHAAHR

Sequences:

>Translated_578_residues
MSALPDIDCDVLVVGSGAAGLSAAVTAAWHGLKVIVVEKDPVFGGATAWSGGWAWVPCNPLAWRAGIVEDVELPRTYLRH
ELGEHYDPAMIDAFLEAGPRMVAFFEQHTALQFADGNAIADIHGETPGAGTGGRSVIAAPYDGRKVGRLLKRLRTTMRET
SFMGMPIMAGADLSAFLNLTRSLSAAWHVTRRFTRHLLDLALHGRAMQLVNGVALVARLGKSAEDLGVLLWESAPVTELL
RDESRITGARVNTTKGPLNVHARKAVVLAAGGFANDIERRRALFPRTPTGHEHWALPPLGVNGDGLRLGETVGGRVNTDV
ASPVAWAPVSQVPHSDGSIGHFPHIIERGKPGVIGVLRNGQRFVNEANGYYDYVSAMVAAAPEGEEVASWLICTRAFQRR
YGLGMSRPFPVPVSGFIRSGYLKTGNTLEELATACGIDPIGLRATLHDYNHHARHGKDPLFGRGSTPYNRKQGDPAQQPN
PCVAPIEQGPFYAVKVQPGCFGTFAGLKVNPHAQVLDTEDRPIPGLYAAGGDMASIMGGHYPAGGINLGPALTFGYIAAR
HMAGVTAFEQEIDHAAHR
>Mature_577_residues
SALPDIDCDVLVVGSGAAGLSAAVTAAWHGLKVIVVEKDPVFGGATAWSGGWAWVPCNPLAWRAGIVEDVELPRTYLRHE
LGEHYDPAMIDAFLEAGPRMVAFFEQHTALQFADGNAIADIHGETPGAGTGGRSVIAAPYDGRKVGRLLKRLRTTMRETS
FMGMPIMAGADLSAFLNLTRSLSAAWHVTRRFTRHLLDLALHGRAMQLVNGVALVARLGKSAEDLGVLLWESAPVTELLR
DESRITGARVNTTKGPLNVHARKAVVLAAGGFANDIERRRALFPRTPTGHEHWALPPLGVNGDGLRLGETVGGRVNTDVA
SPVAWAPVSQVPHSDGSIGHFPHIIERGKPGVIGVLRNGQRFVNEANGYYDYVSAMVAAAPEGEEVASWLICTRAFQRRY
GLGMSRPFPVPVSGFIRSGYLKTGNTLEELATACGIDPIGLRATLHDYNHHARHGKDPLFGRGSTPYNRKQGDPAQQPNP
CVAPIEQGPFYAVKVQPGCFGTFAGLKVNPHAQVLDTEDRPIPGLYAAGGDMASIMGGHYPAGGINLGPALTFGYIAARH
MAGVTAFEQEIDHAAHR

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

Organism=Caenorhabditis elegans, GI71986328, Length=350, Percent_Identity=26.5714285714286, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 61830; Mature: 61699

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALPDIDCDVLVVGSGAAGLSAAVTAAWHGLKVIVVEKDPVFGGATAWSGGWAWVPCNP
CCCCCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCEEEECCCC
LAWRAGIVEDVELPRTYLRHELGEHYDPAMIDAFLEAGPRMVAFFEQHTALQFADGNAIA
HHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEHHCCEEEEECCCEEE
DIHGETPGAGTGGRSVIAAPYDGRKVGRLLKRLRTTMRETSFMGMPIMAGADLSAFLNLT
EECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHH
RSLSAAWHVTRRFTRHLLDLALHGRAMQLVNGVALVARLGKSAEDLGVLLWESAPVTELL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCHHHHH
RDESRITGARVNTTKGPLNVHARKAVVLAAGGFANDIERRRALFPRTPTGHEHWALPPLG
HHHHHCCCCEEECCCCCCEEEEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
VNGDGLRLGETVGGRVNTDVASPVAWAPVSQVPHSDGSIGHFPHIIERGKPGVIGVLRNG
CCCCCEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHCCCCCEEEEECCC
QRFVNEANGYYDYVSAMVAAAPEGEEVASWLICTRAFQRRYGLGMSRPFPVPVSGFIRSG
HHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCC
YLKTGNTLEELATACGIDPIGLRATLHDYNHHARHGKDPLFGRGSTPYNRKQGDPAQQPN
CCCCCCHHHHHHHHHCCCCCCCEEEEHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
PCVAPIEQGPFYAVKVQPGCFGTFAGLKVNPHAQVLDTEDRPIPGLYAAGGDMASIMGGH
CCCCCCCCCCEEEEEECCCCCEEECCCEECCCCEEECCCCCCCCCEEECCCCHHHHHCCC
YPAGGINLGPALTFGYIAARHMAGVTAFEQEIDHAAHR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SALPDIDCDVLVVGSGAAGLSAAVTAAWHGLKVIVVEKDPVFGGATAWSGGWAWVPCNP
CCCCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCEEEECCCC
LAWRAGIVEDVELPRTYLRHELGEHYDPAMIDAFLEAGPRMVAFFEQHTALQFADGNAIA
HHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEHHCCEEEEECCCEEE
DIHGETPGAGTGGRSVIAAPYDGRKVGRLLKRLRTTMRETSFMGMPIMAGADLSAFLNLT
EECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHH
RSLSAAWHVTRRFTRHLLDLALHGRAMQLVNGVALVARLGKSAEDLGVLLWESAPVTELL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCHHHHH
RDESRITGARVNTTKGPLNVHARKAVVLAAGGFANDIERRRALFPRTPTGHEHWALPPLG
HHHHHCCCCEEECCCCCCEEEEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
VNGDGLRLGETVGGRVNTDVASPVAWAPVSQVPHSDGSIGHFPHIIERGKPGVIGVLRNG
CCCCCEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHCCCCCEEEEECCC
QRFVNEANGYYDYVSAMVAAAPEGEEVASWLICTRAFQRRYGLGMSRPFPVPVSGFIRSG
HHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCC
YLKTGNTLEELATACGIDPIGLRATLHDYNHHARHGKDPLFGRGSTPYNRKQGDPAQQPN
CCCCCCHHHHHHHHHCCCCCCCEEEEHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
PCVAPIEQGPFYAVKVQPGCFGTFAGLKVNPHAQVLDTEDRPIPGLYAAGGDMASIMGGH
CCCCCCCCCCEEEEEECCCCCEEECCCEECCCCEEECCCCCCCCCEEECCCCHHHHHCCC
YPAGGINLGPALTFGYIAARHMAGVTAFEQEIDHAAHR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]