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The map label for this gene is dctA1 [H]

Identifier: 77461099

GI number: 77461099

Start: 5501660

End: 5502973

Strand: Direct

Name: dctA1 [H]

Synonym: Pfl01_4878

Alternate gene names: 77461099

Gene position: 5501660-5502973 (Clockwise)

Preceding gene: 77461098

Following gene: 77461100

Centisome position: 85.45

GC content: 60.12

Gene sequence:

>1314_bases
ATGCTCAGATGGTGCTCGCGTTCAATCTTCCTCCAAGTGGTTCTCGGACTGATGCTCGGCATCGTCTGCGGACTGACCCT
TCCCGAATACTCGGCCCAGCTCAAACCGCTCGGCGACGGTTTCATCAAACTGATCAAGATGCTCATCGGCCTGATCGTGT
TCTGCGTGGTGGTCAGCGGCATCAGTGGCGCCGGCGACTTGAAGAAGGTCGGGCGCATCGGCCTCAAGTCGGTTATCTAC
TTCGAAGTACTGACCACCATCGCCCTGGTGATCGGTCTGGTGTTTGCCTTCACCACCGGGATCGGCAGCGGCGCCAATAT
CCATCTGGAGCAGCTGTCCGCCGCCGACATGGGCGACATCGCCGAACGCGGCCAGCACATGCACACCACCACCCAGTTCC
TGATGGACCTGATCCCGACTTCGGTACTCGGCGCCTTTGCCGACAACAACATCCTACAAGTGCTGTTGTTCTCGGTGCTG
TTCGGCAGCGCGTTGAATCTGGTGGGCGAAGCGGCTTCCGGGATCTCGCGGCTGATCAACGAACTGAGCCATGTGATCTT
CCGCATCATGGGCATGATCGTGCGTCTGGCTCCGATCGGCGTGTTCGGCGCCATCGCCTTCACCACCAGCAAATATGGCC
TGGATTCGCTGCAACACCTGGGCAGCCTGGTCGGTTTGTTCTACCTGACCTGCATCGCTTTCGTCGCGCTGATTCTCGGT
CTGGTAATGCGTCTTTCCGGCCTGCGCATGTGGCCGTTGCTCAAGTACCTGCGTGAAGAGTTGCTGATCGTCATGGGCAC
CGCGTCCTCCGACGCCGTGCTGCCGCAGATCATGCGCAAGCTTGAACATCTGGGCATCGGCAGCTCGACTGTCGGGCTGG
TGATTCCCACCGGTTACTCGTTCAACCTCGACGGTTTTTCGATCTACCTGACCCTGGCCATCGTGTTCATCGCCAACGCC
ACCGGTACACCGCTGGCCATGACCGATCTGCTGACGATTTTGCTGGTATCGCTGATTACCTCCAAAGGTGCCCACGGCAT
TCCCGGTTCGGCGCTGGTGATTCTCGCGGCCACGCTGACGGCGATCCCGGCGATCCCGGTGGTCGGTCTGGTGCTGGTAC
TGGCGGTGGACTGGTTCATGGGCATCGGCCGGGCGCTGACCAACCTGATCGGCAACTGCGTCGCCACCGTGGCCATCGCC
CGTTGGGAAAAGGACATCGACGTGCAGCGGGCGAACAAGGTGCTCAACGGCGAGCAGGGCTATAACTTTCAGCCGAGAAA
AACGGTCGCTCAAGCGGCGGCCAAAGAATTCTGA

Upstream 100 bases:

>100_bases
CTGCACCCGCACTGGACATACCGGTAAGACCACAATAATTAAGTCCTGAATCTGATGCGCCCCGCGCACGGCAGCAAGGA
CACCGACCAGGATTCCTCCC

Downstream 100 bases:

>100_bases
ATGAGCGCCGTGCCTGCGCCAATGCAGGCACGGCTCATGGAGCGAACACGTGATTACTACATCAACCGTCGTCAACTCAG
TGGTGGAAAAACTCCGGGCC

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MLRWCSRSIFLQVVLGLMLGIVCGLTLPEYSAQLKPLGDGFIKLIKMLIGLIVFCVVVSGISGAGDLKKVGRIGLKSVIY
FEVLTTIALVIGLVFAFTTGIGSGANIHLEQLSAADMGDIAERGQHMHTTTQFLMDLIPTSVLGAFADNNILQVLLFSVL
FGSALNLVGEAASGISRLINELSHVIFRIMGMIVRLAPIGVFGAIAFTTSKYGLDSLQHLGSLVGLFYLTCIAFVALILG
LVMRLSGLRMWPLLKYLREELLIVMGTASSDAVLPQIMRKLEHLGIGSSTVGLVIPTGYSFNLDGFSIYLTLAIVFIANA
TGTPLAMTDLLTILLVSLITSKGAHGIPGSALVILAATLTAIPAIPVVGLVLVLAVDWFMGIGRALTNLIGNCVATVAIA
RWEKDIDVQRANKVLNGEQGYNFQPRKTVAQAAAKEF

Sequences:

>Translated_437_residues
MLRWCSRSIFLQVVLGLMLGIVCGLTLPEYSAQLKPLGDGFIKLIKMLIGLIVFCVVVSGISGAGDLKKVGRIGLKSVIY
FEVLTTIALVIGLVFAFTTGIGSGANIHLEQLSAADMGDIAERGQHMHTTTQFLMDLIPTSVLGAFADNNILQVLLFSVL
FGSALNLVGEAASGISRLINELSHVIFRIMGMIVRLAPIGVFGAIAFTTSKYGLDSLQHLGSLVGLFYLTCIAFVALILG
LVMRLSGLRMWPLLKYLREELLIVMGTASSDAVLPQIMRKLEHLGIGSSTVGLVIPTGYSFNLDGFSIYLTLAIVFIANA
TGTPLAMTDLLTILLVSLITSKGAHGIPGSALVILAATLTAIPAIPVVGLVLVLAVDWFMGIGRALTNLIGNCVATVAIA
RWEKDIDVQRANKVLNGEQGYNFQPRKTVAQAAAKEF
>Mature_437_residues
MLRWCSRSIFLQVVLGLMLGIVCGLTLPEYSAQLKPLGDGFIKLIKMLIGLIVFCVVVSGISGAGDLKKVGRIGLKSVIY
FEVLTTIALVIGLVFAFTTGIGSGANIHLEQLSAADMGDIAERGQHMHTTTQFLMDLIPTSVLGAFADNNILQVLLFSVL
FGSALNLVGEAASGISRLINELSHVIFRIMGMIVRLAPIGVFGAIAFTTSKYGLDSLQHLGSLVGLFYLTCIAFVALILG
LVMRLSGLRMWPLLKYLREELLIVMGTASSDAVLPQIMRKLEHLGIGSSTVGLVIPTGYSFNLDGFSIYLTLAIVFIANA
TGTPLAMTDLLTILLVSLITSKGAHGIPGSALVILAATLTAIPAIPVVGLVLVLAVDWFMGIGRALTNLIGNCVATVAIA
RWEKDIDVQRANKVLNGEQGYNFQPRKTVAQAAAKEF

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=498, Percent_Identity=25.5020080321285, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI4827012, Length=273, Percent_Identity=30.03663003663, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI40254478, Length=459, Percent_Identity=26.3616557734205, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI194239697, Length=471, Percent_Identity=23.9915074309979, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI66773030, Length=473, Percent_Identity=23.8900634249471, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI21314632, Length=406, Percent_Identity=27.3399014778325, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI223468566, Length=253, Percent_Identity=28.0632411067194, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI223468564, Length=253, Percent_Identity=28.0632411067194, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI5032093, Length=379, Percent_Identity=24.802110817942, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI262359914, Length=394, Percent_Identity=23.8578680203046, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI301601644, Length=161, Percent_Identity=29.8136645962733, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1789947, Length=412, Percent_Identity=49.7572815533981, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1790514, Length=417, Percent_Identity=36.6906474820144, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1789473, Length=328, Percent_Identity=25, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788024, Length=396, Percent_Identity=22.7272727272727, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17537407, Length=473, Percent_Identity=26.4270613107822, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71996953, Length=471, Percent_Identity=24.203821656051, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI193206505, Length=471, Percent_Identity=23.9915074309979, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71983106, Length=461, Percent_Identity=23.4273318872017, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71983099, Length=461, Percent_Identity=23.4273318872017, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI193206654, Length=433, Percent_Identity=21.2471131639723, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17541374, Length=398, Percent_Identity=22.6130653266332, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583025, Length=417, Percent_Identity=24.4604316546763, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17137668, Length=417, Percent_Identity=24.4604316546763, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24583023, Length=417, Percent_Identity=24.4604316546763, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281360481, Length=466, Percent_Identity=25.3218884120172, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI281360483, Length=464, Percent_Identity=25, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17137666, Length=464, Percent_Identity=25, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46544; Mature: 46544

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCHHHHHHHHHHHCC
>Mature Secondary Structure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CCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA