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The map label for this gene is ispB [H]

Identifier: 77461082

GI number: 77461082

Start: 5480185

End: 5481153

Strand: Direct

Name: ispB [H]

Synonym: Pfl01_4861

Alternate gene names: 77461082

Gene position: 5480185-5481153 (Clockwise)

Preceding gene: 77461076

Following gene: 77461083

Centisome position: 85.12

GC content: 63.47

Gene sequence:

>969_bases
ATGCAACCCCAAGCTTTCTACCGCGCGGTGGCGGACGATTTTAGCGCCGTCGACGGCATCATCAAGAAGCAGCTGACTTC
CCGAGTGCCGCTGGTATCGAAAATCGGCGATTACATCACGTCGGCCGGCGGCAAACGCCTGCGTCCTTTATTGGTGCTGC
TGTGCGGCAAGGCCCTGGGTCGCGAAGGCGACGACATGCGCCTGCTGGCCGCTACCATCGAATTCCTGCACACCGCCACC
CTGCTGCATGACGACGTGGTCGACATGTCCGGCATGCGCCGTGGCCGTTCGACCGCCAACGCCATGTGGGGCAACGCCCC
GAGCGTGCTGGTCGGCGACTTCCTGTACTCGCGCTCGTTCGAGATGATGGTCGAACTGGGCTCGATGCCGGTGATGAAAA
TCCTGTCCCAGGCCACGCGCATCATCGCCGAAGGCGAAGTGCTGCAACTGTCCAAGGTTCGCGACGCCAGCACCACCGAA
GAAACCTACATGGAAGTCATCCGCGGCAAGACCGCGATGCTCTTCGAGGCTTCGACACACAGCGCCGCAGCACTGGCTGG
CGCGACCGCCGAGCAGAGCGAAGCCCTGCGCACTTTCGGTGATCACCTGGGCGTTGCGTTCCAGCTGGTCGACGACCTGC
TCGACTACAAGGGCGACGCCGAAACCCTGGGCAAGAACGTCGGTGACGATCTGGCCGAAGGCAAGCCGACCCTGCCGCTG
ATCTACACCATGCGCGAAGGCACGCCGGAGCAAGCCGCACTGGTACGCCAGGCGATCCAGAAAGGCGGGATCGAAGACCT
GGAAAGCATCCGTATCGCCGTGGAAGCCTCCGGTTCGCTGGAATACACCGCGCAACTGGCCCGTGATTACGTGGCTCGCG
CGATCAAGTGCCTCGACGCGCTGCCGGCCAGCGAATACCGCGATGCACTGGTTGAGCTGAGCGAGTTCGCGGTCGCCCGC
ACGCACTGA

Upstream 100 bases:

>100_bases
CAAGCCTTCGAAGTCATGCAAGGGGCCGTCCATCGCGCCTTGACAGGCCCGGACGTGGGTCCTAGCATGCCGCGCAACCC
TTCTGGAGCACCTGTCGCTG

Downstream 100 bases:

>100_bases
TCAGCATCGCGTCATCAAAAAACCGCAGCCTCCGTTGGAGCCTGCGGTTTTTTGTTGCCTGCCATCAGCGGTTAAAACCC
TATACAATGTGCGACTTTTA

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MQPQAFYRAVADDFSAVDGIIKKQLTSRVPLVSKIGDYITSAGGKRLRPLLVLLCGKALGREGDDMRLLAATIEFLHTAT
LLHDDVVDMSGMRRGRSTANAMWGNAPSVLVGDFLYSRSFEMMVELGSMPVMKILSQATRIIAEGEVLQLSKVRDASTTE
ETYMEVIRGKTAMLFEASTHSAAALAGATAEQSEALRTFGDHLGVAFQLVDDLLDYKGDAETLGKNVGDDLAEGKPTLPL
IYTMREGTPEQAALVRQAIQKGGIEDLESIRIAVEASGSLEYTAQLARDYVARAIKCLDALPASEYRDALVELSEFAVAR
TH

Sequences:

>Translated_322_residues
MQPQAFYRAVADDFSAVDGIIKKQLTSRVPLVSKIGDYITSAGGKRLRPLLVLLCGKALGREGDDMRLLAATIEFLHTAT
LLHDDVVDMSGMRRGRSTANAMWGNAPSVLVGDFLYSRSFEMMVELGSMPVMKILSQATRIIAEGEVLQLSKVRDASTTE
ETYMEVIRGKTAMLFEASTHSAAALAGATAEQSEALRTFGDHLGVAFQLVDDLLDYKGDAETLGKNVGDDLAEGKPTLPL
IYTMREGTPEQAALVRQAIQKGGIEDLESIRIAVEASGSLEYTAQLARDYVARAIKCLDALPASEYRDALVELSEFAVAR
TH
>Mature_322_residues
MQPQAFYRAVADDFSAVDGIIKKQLTSRVPLVSKIGDYITSAGGKRLRPLLVLLCGKALGREGDDMRLLAATIEFLHTAT
LLHDDVVDMSGMRRGRSTANAMWGNAPSVLVGDFLYSRSFEMMVELGSMPVMKILSQATRIIAEGEVLQLSKVRDASTTE
ETYMEVIRGKTAMLFEASTHSAAALAGATAEQSEALRTFGDHLGVAFQLVDDLLDYKGDAETLGKNVGDDLAEGKPTLPL
IYTMREGTPEQAALVRQAIQKGGIEDLESIRIAVEASGSLEYTAQLARDYVARAIKCLDALPASEYRDALVELSEFAVAR
TH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=307, Percent_Identity=30.9446254071661, Blast_Score=154, Evalue=8e-38,
Organism=Homo sapiens, GI4758430, Length=269, Percent_Identity=28.6245353159851, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI83700220, Length=269, Percent_Identity=28.6245353159851, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI169808399, Length=336, Percent_Identity=22.9166666666667, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=52.9595015576324, Blast_Score=343, Evalue=9e-96,
Organism=Escherichia coli, GI1786623, Length=272, Percent_Identity=29.0441176470588, Blast_Score=85, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17505681, Length=334, Percent_Identity=32.0359281437126, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319475, Length=300, Percent_Identity=31.3333333333333, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325188, Length=276, Percent_Identity=23.9130434782609, Blast_Score=92, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24651612, Length=299, Percent_Identity=30.7692307692308, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI281365769, Length=280, Percent_Identity=25, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24660002, Length=280, Percent_Identity=25, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 34907; Mature: 34907

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPQAFYRAVADDFSAVDGIIKKQLTSRVPLVSKIGDYITSAGGKRLRPLLVLLCGKALG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
REGDDMRLLAATIEFLHTATLLHDDVVDMSGMRRGRSTANAMWGNAPSVLVGDFLYSRSF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHH
EMMVELGSMPVMKILSQATRIIAEGEVLQLSKVRDASTTEETYMEVIRGKTAMLFEASTH
HHHHHHCCCHHHHHHHHHHHHHHCCCEEEHHHHCCCCCHHHHHHHHHCCCCEEEEECCCC
SAAALAGATAEQSEALRTFGDHLGVAFQLVDDLLDYKGDAETLGKNVGDDLAEGKPTLPL
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHCCCCCCEE
IYTMREGTPEQAALVRQAIQKGGIEDLESIRIAVEASGSLEYTAQLARDYVARAIKCLDA
EEEECCCCCHHHHHHHHHHHHCCHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHC
LPASEYRDALVELSEFAVARTH
CCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQPQAFYRAVADDFSAVDGIIKKQLTSRVPLVSKIGDYITSAGGKRLRPLLVLLCGKALG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
REGDDMRLLAATIEFLHTATLLHDDVVDMSGMRRGRSTANAMWGNAPSVLVGDFLYSRSF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHH
EMMVELGSMPVMKILSQATRIIAEGEVLQLSKVRDASTTEETYMEVIRGKTAMLFEASTH
HHHHHHCCCHHHHHHHHHHHHHHCCCEEEHHHHCCCCCHHHHHHHHHCCCCEEEEECCCC
SAAALAGATAEQSEALRTFGDHLGVAFQLVDDLLDYKGDAETLGKNVGDDLAEGKPTLPL
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHCCCCCCEE
IYTMREGTPEQAALVRQAIQKGGIEDLESIRIAVEASGSLEYTAQLARDYVARAIKCLDA
EEEECCCCCHHHHHHHHHHHHCCHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHC
LPASEYRDALVELSEFAVARTH
CCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]