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The map label for this gene is pilB [H]

Identifier: 77461041

GI number: 77461041

Start: 5439714

End: 5441414

Strand: Direct

Name: pilB [H]

Synonym: Pfl01_4820

Alternate gene names: 77461041

Gene position: 5439714-5441414 (Clockwise)

Preceding gene: 77461038

Following gene: 77461042

Centisome position: 84.49

GC content: 60.14

Gene sequence:

>1701_bases
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTACAGGCCGAACTGCTCACGGAAAAAAGCGCCCAGCACGC
CTGGCAGCAGGCCCAGCGCAATCGCCTGTCGCTGGTCAGCTATCTGGTACAAAACAAACTGGTGAAGAGCGCGCAGGTCG
CCGAGATTGCCTCGGAGCACTTCGGCATGGCGTTCCTCGACCTCAACTGTCTCGACAGGGAAACCCAGCCCAAGGGTCTG
GTCAGTGAAAAACTGGTGCGCCAGCACCATGCCCTGCCCCTGTGGCGTCGCGGCAACAAGCTGTTCGTGGGGATCTCCGA
CCCGAGCAATCATCAGGCGATCAACGACATCCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AGCTCACCGACGCCATCGAGAAATTCTTCGACACCCACGCCTCCGGCCTGGAAGACATGGCCGATGTCGACCTCGACGGG
CTGGACGTCGAGTCCATCGATGACAGCAAGCAGGACGCCATCGGCGGGCTCGATGCCGACGATGCGCCGGTGGTGCGCTT
CGTGCACAAGATGCTGCTGGACGCAATCAAGAGCGGCTCTTCCGACCTGCACTTCGAGCCTTACGAGAAAAACTACCGGG
TGCGGGTGCGCACCGACGGCATCCTGCGCGAAGTGGCCAAGCCACCGATTCAATTGGCAGGACGCATCGCCGCACGCCTG
AAGGTGATGGCCAGCCTCGACATCTCGGAGCGCCGCAAACCTCAGGACGGGCGCCTGAAGATGCGCCTGTCAAAGACCAA
ATCGATCGATTTCCGGGTCAACACCCTGCCAACCCTGTGGGGCGAAAAAGTGGTGATCCGGATCCTCGACCCCTCCAGCG
CGCAGATCGGCATCGACGCCCTCGGTTATGAGCCGGAGCAGAAAGATCTGTACATGGCCGCCCTGAAACAGCCGCAAGGA
ATGATTCTGGTCACCGGCCCCACCGGTTCGGGCAAGACCGTTTCGCTGTACACCGGGCTGAATATCCTCAATACCGTCGA
CATCAACATCTCTACCGCTGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAAG
GGATGGACTTTGCCCAGGCCCTGCGCTCGTTCCTGCGTCAGGATCCGGACGTGATCATGGTCGGCGAGATCCGTGACCTC
GAGACCGCCGAGATCGCCATCAAAGCAGCGCAGACCGGTCACCTCGTGCTCTCCACGCTGCACACCAACAGCGCCGCCGA
GACACTCACCCGCTTGCACAACATGGGCATTCCCGGCTTCAACATCGCCACGTCGGTGAGCCTGATCATCGCCCAGCGTC
TGGCGCGAAAACTCTGCAGCCAATGCAAGAAAGCCATCGAGATTCCCCGCGAAACCTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCAGTTTCACAATCTATGAGCCCGTCGGCTGCGATCACTGCAACGGCGGGTACAAGGGTCGCGTGGGGAT
TTATGAAGTGGTGAAGAACACTCCCGAGTTGCAACGGCTGATCATGGCCGAGGGCAACTCACTGGAAATCGATACCCGGA
TGCGCCGGGACGGCTTCAACGACCTGCGCACCTCGGGCCTGATCAAAGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Upstream 100 bases:

>100_bases
AACCGCACTATCTGACACTTTTTGTCACCTGCACCCCGCGTGTTTGGCGCTGTCACTTGACTAGGCTATAAGTCATGAAC
TGTCTGCATCCGGGATTCCC

Downstream 100 bases:

>100_bases
ACATGGCCGTCAAGGCAGCGAAAATCAGTGTCTATGCCTGGGAAGGCACGGACCGCAAAGGCAGCAAGGTGACCGGCGAG
CTGAGCGGCCAGAACCCCGC

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MNDIALSGLAKQLVQAELLTEKSAQHAWQQAQRNRLSLVSYLVQNKLVKSAQVAEIASEHFGMAFLDLNCLDRETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHASGLEDMADVDLDG
LDVESIDDSKQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSQCKKAIEIPRETLLKEGFPE
ERIGSFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDTRMRRDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Sequences:

>Translated_566_residues
MNDIALSGLAKQLVQAELLTEKSAQHAWQQAQRNRLSLVSYLVQNKLVKSAQVAEIASEHFGMAFLDLNCLDRETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHASGLEDMADVDLDG
LDVESIDDSKQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSQCKKAIEIPRETLLKEGFPE
ERIGSFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDTRMRRDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD
>Mature_566_residues
MNDIALSGLAKQLVQAELLTEKSAQHAWQQAQRNRLSLVSYLVQNKLVKSAQVAEIASEHFGMAFLDLNCLDRETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHASGLEDMADVDLDG
LDVESIDDSKQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSQCKKAIEIPRETLLKEGFPE
ERIGSFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDTRMRRDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=384, Percent_Identity=47.3958333333333, Blast_Score=353, Evalue=1e-98,
Organism=Escherichia coli, GI1786296, Length=385, Percent_Identity=45.4545454545455, Blast_Score=346, Evalue=3e-96,
Organism=Escherichia coli, GI87082188, Length=183, Percent_Identity=38.7978142076503, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62669; Mature: 62669

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIALSGLAKQLVQAELLTEKSAQHAWQQAQRNRLSLVSYLVQNKLVKSAQVAEIASEH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGMAFLDLNCLDRETQPKGLVSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFS
CCEEEEECHHCCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCCEEEC
TGLSTEAILVEDDKLTDAIEKFFDTHASGLEDMADVDLDGLDVESIDDSKQDAIGGLDAD
CCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCC
DAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHCCCHHHHHHHHHHHH
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDA
HHHHHCCHHHCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCEEEEEEECCCCCEECCCC
LGYEPEQKDLYMAALKQPQGMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEIN
CCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCEEEEEEEEEECCCCCEEEE
MEGINQVNVNPRQGMDFAQALRSFLRQDPDVIMVGEIRDLETAEIAIKAAQTGHLVLSTL
ECCCCEECCCHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECCCCCEEEEEE
HTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSQCKKAIEIPRETLLKEGFPE
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCH
ERIGSFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDTRMRRDGFN
HHCCCEEEECCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCEEECHHHHHCCCH
DLRTSGLIKAMQGITSLEEINRVTKD
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNDIALSGLAKQLVQAELLTEKSAQHAWQQAQRNRLSLVSYLVQNKLVKSAQVAEIASEH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGMAFLDLNCLDRETQPKGLVSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFS
CCEEEEECHHCCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCCEEEC
TGLSTEAILVEDDKLTDAIEKFFDTHASGLEDMADVDLDGLDVESIDDSKQDAIGGLDAD
CCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCC
DAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHCCCHHHHHHHHHHHH
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDA
HHHHHCCHHHCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCEEEEEEECCCCCEECCCC
LGYEPEQKDLYMAALKQPQGMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEIN
CCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCEEEEEEEEEECCCCCEEEE
MEGINQVNVNPRQGMDFAQALRSFLRQDPDVIMVGEIRDLETAEIAIKAAQTGHLVLSTL
ECCCCEECCCHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECCCCCEEEEEE
HTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSQCKKAIEIPRETLLKEGFPE
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCH
ERIGSFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDTRMRRDGFN
HHCCCEEEECCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCEEECHHHHHCCCH
DLRTSGLIKAMQGITSLEEINRVTKD
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]