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The map label for this gene is pcnB [H]

Identifier: 77461030

GI number: 77461030

Start: 5425764

End: 5427164

Strand: Direct

Name: pcnB [H]

Synonym: Pfl01_4809

Alternate gene names: 77461030

Gene position: 5425764-5427164 (Clockwise)

Preceding gene: 77461029

Following gene: 77461031

Centisome position: 84.27

GC content: 62.31

Gene sequence:

>1401_bases
ATGCTGAAGAAGCTGTTCCAGTCATTCCGAACTCCCCTGCGTCGTACGCAACACATCCGTAGCACGCCTGAAGTCCTCAA
CAGCGGCCAACATTCGCTGCAGAAGGCGCAATTCAGCCGTTACGCGGTCAACATCGTCGAACGTCTGCAGGGCGCCGGCT
ACCAGGCCTACCTGGTTGGCGGTTGTGTGCGAGACATGCTGCTGGGCATCACGCCCAAAGACTTCGACGTCGCCACCAGC
GCCACCCCCGAGCAGGTTCGTGCCGAATTCCGCAACGCGCGGATCATCGGCCGCCGCTTCAAGCTGGTACACATCCATTT
CGGTCGCGAAATCATCGAAGTCGCGACCTTCCGCGCCAATCACCCGCAAAACGAAGACGACGAAGACAGCAACCAGTCTT
CGCGCAACGAGAGCGGGCGCATTCTGCGTGACAACGTCTACGGCACCCTGGAAGAAGACGCGCAACGCCGCGACTTCACC
ATCAACGCCCTGTATTACGATCCGGTCAGCGAGCGCATCCTCGACTACGCCAACGGCGTGCACGACATCCGCAATCACCT
GATCCGTCTGATCGGCGACCCGAAGCAGCGTTACCAGGAAGACCCGGTGCGGATGCTGCGGGCCGTGCGTTTTGCCGCCA
AGCTCAATTTCGGTATCGAGAAGCACACCGTCCAGCCAATCCGCGAACTGGCGCCGATGCTGCGCGAGATCCCGTCGGCC
CGCCTGTTCGAGGAAGTGCTCAAGCTGTTCCTCTCCGGTCACGGTGCCATCACCTTCGAGATGCTGGTCGACCTGCAACT
GTTCGCCCCGCTGTTCCCGGCCAGTGCCGATGCGCTGGAACACAACCCTGAATACACCCACACGCTGATCAGCGAAGCAC
TGACCAACACCGATCTGCGGATCAAGCAGAACAAACCGGTGACCCCGGCGTTCCTGTTCGCCGCGCTGCTGTGGCCTGCC
CTGCCGGCCCGCGTGTTGCGTCTGCAAGAGCGTGGCATGCCGCCGATTCCGGCGATGCAGGAAGGCGCTCACGAACTGAT
CGCCGAACAGTGCCAGCGCATTGCGATTCCAAAACGTTTCACCATGCCGATCCGCGAAATCTGGGACATGCAGGAACGCC
TGCCACGCCGCAGCGGCAAACGCGCCGATCTGCTGCTGGACAATCCGCGCTTCCGCGCCGGCTACGACTTCCTGCTGCTG
CGTGAAAGCGCCGGAGAGCAGACCGATGGCCTGGGCGAATGGTGGACCGATTATCAGGACGCCAACGACAGCGAACGTCG
CGACATGATCCGAGACCTCAGCGGCAAGGGTGATGACGCCAGTGGCGCACCGCGCAAACGTCGCCGCAGCAGCGGTTCCA
AGCGCAAACGTGCCGGCGCACCGAGCGCCACGGGCGAATAA

Upstream 100 bases:

>100_bases
GGCAGCGTCCTACACCATCCCTCGACTAAATGCTAGAATCCCGGCCCATCATGCGGTCATTCTCTGGTATGGCCGAACAT
TCCTTCAAACAGTGCATCCC

Downstream 100 bases:

>100_bases
GGCATGGAACGCATCTACATTGGCATGGGCAGCAACCTGGCTGACCCGGCCGAACAATTGCGCAGCGCGGTCGACGCACT
GAAGCAATTGCCCGACACCG

Product: Poly(A) polymerase, PcnB

Products: NA

Alternate protein names: PAP [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MLKKLFQSFRTPLRRTQHIRSTPEVLNSGQHSLQKAQFSRYAVNIVERLQGAGYQAYLVGGCVRDMLLGITPKDFDVATS
ATPEQVRAEFRNARIIGRRFKLVHIHFGREIIEVATFRANHPQNEDDEDSNQSSRNESGRILRDNVYGTLEEDAQRRDFT
INALYYDPVSERILDYANGVHDIRNHLIRLIGDPKQRYQEDPVRMLRAVRFAAKLNFGIEKHTVQPIRELAPMLREIPSA
RLFEEVLKLFLSGHGAITFEMLVDLQLFAPLFPASADALEHNPEYTHTLISEALTNTDLRIKQNKPVTPAFLFAALLWPA
LPARVLRLQERGMPPIPAMQEGAHELIAEQCQRIAIPKRFTMPIREIWDMQERLPRRSGKRADLLLDNPRFRAGYDFLLL
RESAGEQTDGLGEWWTDYQDANDSERRDMIRDLSGKGDDASGAPRKRRRSSGSKRKRAGAPSATGE

Sequences:

>Translated_466_residues
MLKKLFQSFRTPLRRTQHIRSTPEVLNSGQHSLQKAQFSRYAVNIVERLQGAGYQAYLVGGCVRDMLLGITPKDFDVATS
ATPEQVRAEFRNARIIGRRFKLVHIHFGREIIEVATFRANHPQNEDDEDSNQSSRNESGRILRDNVYGTLEEDAQRRDFT
INALYYDPVSERILDYANGVHDIRNHLIRLIGDPKQRYQEDPVRMLRAVRFAAKLNFGIEKHTVQPIRELAPMLREIPSA
RLFEEVLKLFLSGHGAITFEMLVDLQLFAPLFPASADALEHNPEYTHTLISEALTNTDLRIKQNKPVTPAFLFAALLWPA
LPARVLRLQERGMPPIPAMQEGAHELIAEQCQRIAIPKRFTMPIREIWDMQERLPRRSGKRADLLLDNPRFRAGYDFLLL
RESAGEQTDGLGEWWTDYQDANDSERRDMIRDLSGKGDDASGAPRKRRRSSGSKRKRAGAPSATGE
>Mature_466_residues
MLKKLFQSFRTPLRRTQHIRSTPEVLNSGQHSLQKAQFSRYAVNIVERLQGAGYQAYLVGGCVRDMLLGITPKDFDVATS
ATPEQVRAEFRNARIIGRRFKLVHIHFGREIIEVATFRANHPQNEDDEDSNQSSRNESGRILRDNVYGTLEEDAQRRDFT
INALYYDPVSERILDYANGVHDIRNHLIRLIGDPKQRYQEDPVRMLRAVRFAAKLNFGIEKHTVQPIRELAPMLREIPSA
RLFEEVLKLFLSGHGAITFEMLVDLQLFAPLFPASADALEHNPEYTHTLISEALTNTDLRIKQNKPVTPAFLFAALLWPA
LPARVLRLQERGMPPIPAMQEGAHELIAEQCQRIAIPKRFTMPIREIWDMQERLPRRSGKRADLLLDNPRFRAGYDFLLL
RESAGEQTDGLGEWWTDYQDANDSERRDMIRDLSGKGDDASGAPRKRRRSSGSKRKRAGAPSATGE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=252, Percent_Identity=29.3650793650794, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI87081691, Length=413, Percent_Identity=47.6997578692494, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI1789436, Length=334, Percent_Identity=29.3413173652695, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321016, Length=173, Percent_Identity=29.4797687861272, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281360112, Length=237, Percent_Identity=28.6919831223629, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21357337, Length=237, Percent_Identity=28.6919831223629, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 53286; Mature: 53286

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKLFQSFRTPLRRTQHIRSTPEVLNSGQHSLQKAQFSRYAVNIVERLQGAGYQAYLVG
CHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHH
GCVRDMLLGITPKDFDVATSATPEQVRAEFRNARIIGRRFKLVHIHFGREIIEVATFRAN
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHEECEEEEEEEEHHHHHHHHHHHHCC
HPQNEDDEDSNQSSRNESGRILRDNVYGTLEEDAQRRDFTINALYYDPVSERILDYANGV
CCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCEEEEEEEECCHHHHHHHHHCCH
HDIRNHLIRLIGDPKQRYQEDPVRMLRAVRFAAKLNFGIEKHTVQPIRELAPMLREIPSA
HHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
RLFEEVLKLFLSGHGAITFEMLVDLQLFAPLFPASADALEHNPEYTHTLISEALTNTDLR
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHCCCCEE
IKQNKPVTPAFLFAALLWPALPARVLRLQERGMPPIPAMQEGAHELIAEQCQRIAIPKRF
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
TMPIREIWDMQERLPRRSGKRADLLLDNPRFRAGYDFLLLRESAGEQTDGLGEWWTDYQD
CCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCC
ANDSERRDMIRDLSGKGDDASGAPRKRRRSSGSKRKRAGAPSATGE
CCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MLKKLFQSFRTPLRRTQHIRSTPEVLNSGQHSLQKAQFSRYAVNIVERLQGAGYQAYLVG
CHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHH
GCVRDMLLGITPKDFDVATSATPEQVRAEFRNARIIGRRFKLVHIHFGREIIEVATFRAN
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHEECEEEEEEEEHHHHHHHHHHHHCC
HPQNEDDEDSNQSSRNESGRILRDNVYGTLEEDAQRRDFTINALYYDPVSERILDYANGV
CCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCEEEEEEEECCHHHHHHHHHCCH
HDIRNHLIRLIGDPKQRYQEDPVRMLRAVRFAAKLNFGIEKHTVQPIRELAPMLREIPSA
HHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
RLFEEVLKLFLSGHGAITFEMLVDLQLFAPLFPASADALEHNPEYTHTLISEALTNTDLR
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHCCCCEE
IKQNKPVTPAFLFAALLWPALPARVLRLQERGMPPIPAMQEGAHELIAEQCQRIAIPKRF
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
TMPIREIWDMQERLPRRSGKRADLLLDNPRFRAGYDFLLLRESAGEQTDGLGEWWTDYQD
CCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCC
ANDSERRDMIRDLSGKGDDASGAPRKRRRSSGSKRKRAGAPSATGE
CCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]