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The map label for this gene is imm [H]

Identifier: 77460983

GI number: 77460983

Start: 5367736

End: 5367990

Strand: Direct

Name: imm [H]

Synonym: Pfl01_4762

Alternate gene names: 77460983

Gene position: 5367736-5367990 (Clockwise)

Preceding gene: 77460982

Following gene: 77460985

Centisome position: 83.37

GC content: 46.67

Gene sequence:

>255_bases
ATGAAAAAAACCATTTCTGACTACACAGAAGCTGAGTTCATTGAGTTTCTGAAATCCGTGCTGGCGGCCAATACAAGTCC
CGATGAAATACTTGATCCGTTACTTTTTGAGTTTGAACGTCTCAGTGAGCACCCGGCAGGATCTGACTTGATTTATTGGC
CGGAGGATTTGAGCAAAAGAGTGACAGCCGAAGGCATCACAGAAACGGTGAAAGAGTGGCGTGCTGCCAACGGCCTACCT
GGTTTCAAGCAGTAA

Upstream 100 bases:

>100_bases
ACGTCGAGCATATTGCCAATAACGGCGCGGTATACGACATAGACAACTTGCGAGTGAACACTCCAAAAAATCACGTTGAA
AACCACCGAAGTGGATAGCC

Downstream 100 bases:

>100_bases
ACATCTGGAATCCATACTCCAAACAGCTAAAACGGCTTACCGAAAGGCAAGCCGTTTGTTTATACCTCTTCCCCCAAGGC
CAAGAGCCCCTTCGCCCAAA

Product: colicin immunity protein/pyocin immunity protein

Products: NA

Alternate protein names: ImmE2; Microcin-E2 immunity protein [H]

Number of amino acids: Translated: 84; Mature: 84

Protein sequence:

>84_residues
MKKTISDYTEAEFIEFLKSVLAANTSPDEILDPLLFEFERLSEHPAGSDLIYWPEDLSKRVTAEGITETVKEWRAANGLP
GFKQ

Sequences:

>Translated_84_residues
MKKTISDYTEAEFIEFLKSVLAANTSPDEILDPLLFEFERLSEHPAGSDLIYWPEDLSKRVTAEGITETVKEWRAANGLP
GFKQ
>Mature_84_residues
MKKTISDYTEAEFIEFLKSVLAANTSPDEILDPLLFEFERLSEHPAGSDLIYWPEDLSKRVTAEGITETVKEWRAANGLP
GFKQ

Specific function: This protein is able to protect a cell, which harbors the plasmid ColE2 encoding colicin E2, against colicin E2 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the colicins ColE2/ColE8/ColE9 and pyocins S1/S2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000290
- ProDom:   PD007225 [H]

Pfam domain/function: PF01320 Colicin_Pyocin [H]

EC number: NA

Molecular weight: Translated: 9533; Mature: 9533

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTISDYTEAEFIEFLKSVLAANTSPDEILDPLLFEFERLSEHPAGSDLIYWPEDLSKR
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHH
VTAEGITETVKEWRAANGLPGFKQ
HHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKKTISDYTEAEFIEFLKSVLAANTSPDEILDPLLFEFERLSEHPAGSDLIYWPEDLSKR
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHH
VTAEGITETVKEWRAANGLPGFKQ
HHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6095211; 2987833; 3892228 [H]