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The map label for this gene is cfa [H]

Identifier: 77460962

GI number: 77460962

Start: 5345983

End: 5347254

Strand: Direct

Name: cfa [H]

Synonym: Pfl01_4741

Alternate gene names: 77460962

Gene position: 5345983-5347254 (Clockwise)

Preceding gene: 77460961

Following gene: 77460963

Centisome position: 83.03

GC content: 61.87

Gene sequence:

>1272_bases
ATGAAATCCACTAGCGTCTCGGCCAAAGCCAGCCTGTTCAGCCCTCACGGCCTGAGCGGTTCGCTGCTGCGCCGGGGAGT
ACTGCGCCAGCTGGAGCAGCTCAAGCAGGGTCAACTGATCGTCATCGAGGACGGCGAGCGCCACGTCTTCGGCACGCCGG
GCAGCGCCCTGGTGGGCGAGATTCAAGTTCTGGATCCGGCCGTCTGGGGCCTGGTCGCCGGCAATGGTTCGATCGGTGCG
GGTGAAGCTTTCATCCATGGCTACTGGCGCTCGCCGGACCTGACGGCCGTGGTGCGGGTCTTCGTCAGCAACCTTGAAGT
GCTCGACGCAATGGAAGGTGGCCTGGCCCGGCTCGGTCGCCCGCTGGTGCGCGGGCTGCACTGGCTCAACCGCAATACGC
GCAAGGGCTCGCAGAAAAACATCGCCGCTCATTACGATCTGGGCAATGACCTGTTCGAGCAGTTTCTTGACCCGACCATG
ATGTATTCAGCCGCGCAGTTCCTCAGCCCTGACGACAGCCTGGAGCAGGCGCAACTGAACAAGCTGGAGCGGATCTGCCA
GAAACTCGCGCTCAAGCCTGGCGACCATCTGCTGGAAATCGGCACCGGTTGGGGCAGCATGGCGCTCTACGCGGCGCAGC
ATTATGGCTGCAAAGTCACTACCACCACGCTGTCGAAGGAGCAGTTCGCCTTCACTGCCGCGCGCATCGAACGTCTCGGT
TTGCAGGATCAAGTGACGCTGCTACTCAAGGACTACCGCGACCTCACCGGCCAGTACGACAAACTGGTATCGATCGAGAT
GATCGAGGCGGTCGGCCACCGCTTCCTGCCGACCTACTTCAAGCAATGCGCGCAGCTGCTCAAGAGCAACGGCCTGATGC
TGCTGCAAGCGATCACCATCCGCGAACAGCGTTACGAACAGGCCCGCCGTGGCGTGGACTTCATCCAGCGCTACATTTTC
CCCGGCGGCGCCCTGCCCTGCGTGCAGAAAATGCTCGAAATCGTCGGCCGCGACACCGACATGAACCTGCTGCACATGGA
AGATTTCGGCCTGCATTACGCCCGCACCCTGCGTCTGTGGCACGAGAACTTTCGCCATGCTCACGGCCGTCTGACCGAAC
TGGGCTATGACGATTACTTCCTCAGGTTGTGGGAGTTCTACCTGTGTTACTGCGAGGGCGGATTCCTCGAGCGCACCATC
GGCACCGCACAACTGGTGCTGGCCAAACCGGCGGCGATGACCGAGCCTTTGCTGGGCCGGTTCGATGCTTGA

Upstream 100 bases:

>100_bases
CAGGCGCTGCGCCTGTTGCTCAAGCGCACCCCGGTGTATGACCACCAACGCGCCGACGACCGTTTTCGAACCGCCACTGT
ACCGACCGAGGAACGCCACC

Downstream 100 bases:

>100_bases
GCGGTTGGCCAACGCGGTGCTGTTCCAGATCGGCTGGCTGGCCTGCGTGCTCGGTGGCGACAGCCTTTGGCTGCTACTGG
CGCTGGCGGTGCTGGTGATT

Product: cyclopropane-fatty-acyl-phospholipid synthase

Products: NA

Alternate protein names: CFA synthase; Cyclopropane fatty acid synthase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MKSTSVSAKASLFSPHGLSGSLLRRGVLRQLEQLKQGQLIVIEDGERHVFGTPGSALVGEIQVLDPAVWGLVAGNGSIGA
GEAFIHGYWRSPDLTAVVRVFVSNLEVLDAMEGGLARLGRPLVRGLHWLNRNTRKGSQKNIAAHYDLGNDLFEQFLDPTM
MYSAAQFLSPDDSLEQAQLNKLERICQKLALKPGDHLLEIGTGWGSMALYAAQHYGCKVTTTTLSKEQFAFTAARIERLG
LQDQVTLLLKDYRDLTGQYDKLVSIEMIEAVGHRFLPTYFKQCAQLLKSNGLMLLQAITIREQRYEQARRGVDFIQRYIF
PGGALPCVQKMLEIVGRDTDMNLLHMEDFGLHYARTLRLWHENFRHAHGRLTELGYDDYFLRLWEFYLCYCEGGFLERTI
GTAQLVLAKPAAMTEPLLGRFDA

Sequences:

>Translated_423_residues
MKSTSVSAKASLFSPHGLSGSLLRRGVLRQLEQLKQGQLIVIEDGERHVFGTPGSALVGEIQVLDPAVWGLVAGNGSIGA
GEAFIHGYWRSPDLTAVVRVFVSNLEVLDAMEGGLARLGRPLVRGLHWLNRNTRKGSQKNIAAHYDLGNDLFEQFLDPTM
MYSAAQFLSPDDSLEQAQLNKLERICQKLALKPGDHLLEIGTGWGSMALYAAQHYGCKVTTTTLSKEQFAFTAARIERLG
LQDQVTLLLKDYRDLTGQYDKLVSIEMIEAVGHRFLPTYFKQCAQLLKSNGLMLLQAITIREQRYEQARRGVDFIQRYIF
PGGALPCVQKMLEIVGRDTDMNLLHMEDFGLHYARTLRLWHENFRHAHGRLTELGYDDYFLRLWEFYLCYCEGGFLERTI
GTAQLVLAKPAAMTEPLLGRFDA
>Mature_423_residues
MKSTSVSAKASLFSPHGLSGSLLRRGVLRQLEQLKQGQLIVIEDGERHVFGTPGSALVGEIQVLDPAVWGLVAGNGSIGA
GEAFIHGYWRSPDLTAVVRVFVSNLEVLDAMEGGLARLGRPLVRGLHWLNRNTRKGSQKNIAAHYDLGNDLFEQFLDPTM
MYSAAQFLSPDDSLEQAQLNKLERICQKLALKPGDHLLEIGTGWGSMALYAAQHYGCKVTTTTLSKEQFAFTAARIERLG
LQDQVTLLLKDYRDLTGQYDKLVSIEMIEAVGHRFLPTYFKQCAQLLKSNGLMLLQAITIREQRYEQARRGVDFIQRYIF
PGGALPCVQKMLEIVGRDTDMNLLHMEDFGLHYARTLRLWHENFRHAHGRLTELGYDDYFLRLWEFYLCYCEGGFLERTI
GTAQLVLAKPAAMTEPLLGRFDA

Specific function: Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge [H]

COG id: COG2230

COG function: function code M; Cyclopropane fatty acid synthase and related methyltransferases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CFA/CMAS family [H]

Homologues:

Organism=Escherichia coli, GI1787951, Length=360, Percent_Identity=37.5, Blast_Score=197, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI133904226, Length=306, Percent_Identity=39.5424836601307, Blast_Score=221, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003333 [H]

Pfam domain/function: PF02353 CMAS [H]

EC number: =2.1.1.79 [H]

Molecular weight: Translated: 47525; Mature: 47525

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTSVSAKASLFSPHGLSGSLLRRGVLRQLEQLKQGQLIVIEDGERHVFGTPGSALVGE
CCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCHHHHH
IQVLDPAVWGLVAGNGSIGAGEAFIHGYWRSPDLTAVVRVFVSNLEVLDAMEGGLARLGR
HHEECHHHHEEEECCCCCCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCH
PLVRGLHWLNRNTRKGSQKNIAAHYDLGNDLFEQFLDPTMMYSAAQFLSPDDSLEQAQLN
HHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHH
KLERICQKLALKPGDHLLEIGTGWGSMALYAAQHYGCKVTTTTLSKEQFAFTAARIERLG
HHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHC
LQDQVTLLLKDYRDLTGQYDKLVSIEMIEAVGHRFLPTYFKQCAQLLKSNGLMLLQAITI
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHH
REQRYEQARRGVDFIQRYIFPGGALPCVQKMLEIVGRDTDMNLLHMEDFGLHYARTLRLW
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHCHHHHHHHHHH
HENFRHAHGRLTELGYDDYFLRLWEFYLCYCEGGFLERTIGTAQLVLAKPAAMTEPLLGR
HHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHCCCHHHHCHHHHC
FDA
CCC
>Mature Secondary Structure
MKSTSVSAKASLFSPHGLSGSLLRRGVLRQLEQLKQGQLIVIEDGERHVFGTPGSALVGE
CCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCHHHHH
IQVLDPAVWGLVAGNGSIGAGEAFIHGYWRSPDLTAVVRVFVSNLEVLDAMEGGLARLGR
HHEECHHHHEEEECCCCCCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCH
PLVRGLHWLNRNTRKGSQKNIAAHYDLGNDLFEQFLDPTMMYSAAQFLSPDDSLEQAQLN
HHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHH
KLERICQKLALKPGDHLLEIGTGWGSMALYAAQHYGCKVTTTTLSKEQFAFTAARIERLG
HHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHC
LQDQVTLLLKDYRDLTGQYDKLVSIEMIEAVGHRFLPTYFKQCAQLLKSNGLMLLQAITI
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHH
REQRYEQARRGVDFIQRYIFPGGALPCVQKMLEIVGRDTDMNLLHMEDFGLHYARTLRLW
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHCHHHHHHHHHH
HENFRHAHGRLTELGYDDYFLRLWEFYLCYCEGGFLERTIGTAQLVLAKPAAMTEPLLGR
HHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHCCCHHHHCHHHHC
FDA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]