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The map label for this gene is alkK [H]

Identifier: 77460930

GI number: 77460930

Start: 5317074

End: 5318756

Strand: Reverse

Name: alkK [H]

Synonym: Pfl01_4709

Alternate gene names: 77460930

Gene position: 5318756-5317074 (Counterclockwise)

Preceding gene: 77460931

Following gene: 77460929

Centisome position: 82.61

GC content: 61.62

Gene sequence:

>1683_bases
ATGTTGCAGACTCGCGTTATTCCGCCCGCCGAAGGGGCGTACCAGTATCCACTGCTGATCAAACGGCTGCTGATGTCGGG
CGCCCGTTACGAGAAAACCCGCGAGATCATCTACCGTGACCAACTGCGCTACAGCTACCCGACCCTGATCGAACGGGTCG
CGCAGCTGGCCAACGTGCTGACGGCGGCCGGGGTCAAGGCCGGTGACACCGTGGCGGTGATGGACTGGGACAGCCATCGC
TACCTGGAGTGCATGTTTGCGATTCCGATGATCGGCGCGGTGATCCACACCATCAACGTGCGCCTGTCGCCGGAACAGAT
CCTCTACACCATGAACCACGCCGAGGACCGCTTCGTGCTGGTCAACAGCGAGTTCGTCGGGCTGTATCAGGCCATTGCGC
CACACCTGACCACGGTGGAAAAAACCCTGCTGCTGACCGATCTGCCGGAAAAAACCGCCGATCTGCCGAATCTGGTGGGC
GAGTACGAGCAACTGCTGGCGGCCGCGAGTCCGCAGTACGACTTCCTGGATTTCGACGAGAACTCGGTCGCCACCACGTT
CTACACCACGGGTACCACCGGCAATCCGAAAGGCGTGTATTTCACCCATCGGCAACTGGTGCTGCACACCATGGGCGTGT
CGACCATCATGGGCGCCATCGACAGCGTGCGGCTGCTGGGCACCAACGACGTGTACATGCCAATCACCCCGATGTTCCAT
GTGCATGCGTGGGGCCTGCCTTACGTGGCGACCATGCTCGGGCTGAAACAGGTTTATCCGGGGCGTTACGACCCGGAATT
CCTGGTCGAGTTGTGGCGCAAGGAAAAGGTCACGTTCTCCCATTGCGTGCCGACTATCCTGCAGATGGTGCTCAACGCCA
AGGGCGCGCAAGGCACCGACTTCGGCGGCTGGAAAATCGTCATCGGCGGCAGCGCGCTCAATCGCACGCTGTATGAAACG
GCCAAGAGCAAGGGCATTCAGCTGACGGCTGCCTATGGCATGTCGGAAACCGGGCCTCTGGTTTCATGTGCGCATCTGAA
CGATGAATTGATGGCCGGCACCGAAGACGAGCGCACCACCTACCGGATCAAGGCCGGTGTGCCGGGGCCATTGGTCGAAG
CGGCGATCGTCGACACCGAGGGCAATTTCCTCCCGGCAGACGGCGAGACCCAGGGCGAACTGGTACTGCGCGCACCGTGG
CTGACCGAGGGCTACTTCAACGAACCGCAGAAGGGCGCCGAGCTGTGGGCCGGCGGCTGGCTGCACACCGGCGACGTAGC
GACGCTCGACGCCATGGGTGTGATCGATATCCGCGACCGGATCAAGGACGTGATCAAGACCGGCGGCGAATGGATCTCCT
CGCTGGACCTCGAAGACCTGATCAGCCGCCACGTCGCGGTACGTGAAGTAGCAGTGGTGGGCATCGCCGATCCGCAGTGG
GGCGAGCGCCCGTTTGCCTTGCTGGTGGTCCGTGAAGGTCACGCCATCGGGGCGCGCGAGCTCAAGGAACACCTCAAGCC
GTTCGTTGAACTGGGGCACTTGAGCAAGTGGGCGATACCTAGTCAGATCGCCCTTGTTACTGAAATTCCCAAGACCAGTG
TCGGCAAGCTCGACAAGAAGCGCATCCGCCTCGACATCACCGAATGGCAGGCCAACAACAGCACCTTCCTCTCGACGCTC
TGA

Upstream 100 bases:

>100_bases
GGCTCTGCAAATCATTCCTTTGGCTGATTTGGCTGACGTATAAGGGTTCTAGAGTACGAAACTCCAAAGCCAACACCGTG
CAGTCAGAAAAGGGATTCTT

Downstream 100 bases:

>100_bases
GCGTCCCCTGGCGCGCCGAAAACGGCGCGCCAGGCCCACCAAGCAAGCGCTTGGCTTGTGAAATTCGAATTTTCAGCCAT
CCTTGCCGTGCCGACATGTG

Product: long-chain-fatty-acid--CoA ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MLQTRVIPPAEGAYQYPLLIKRLLMSGARYEKTREIIYRDQLRYSYPTLIERVAQLANVLTAAGVKAGDTVAVMDWDSHR
YLECMFAIPMIGAVIHTINVRLSPEQILYTMNHAEDRFVLVNSEFVGLYQAIAPHLTTVEKTLLLTDLPEKTADLPNLVG
EYEQLLAAASPQYDFLDFDENSVATTFYTTGTTGNPKGVYFTHRQLVLHTMGVSTIMGAIDSVRLLGTNDVYMPITPMFH
VHAWGLPYVATMLGLKQVYPGRYDPEFLVELWRKEKVTFSHCVPTILQMVLNAKGAQGTDFGGWKIVIGGSALNRTLYET
AKSKGIQLTAAYGMSETGPLVSCAHLNDELMAGTEDERTTYRIKAGVPGPLVEAAIVDTEGNFLPADGETQGELVLRAPW
LTEGYFNEPQKGAELWAGGWLHTGDVATLDAMGVIDIRDRIKDVIKTGGEWISSLDLEDLISRHVAVREVAVVGIADPQW
GERPFALLVVREGHAIGARELKEHLKPFVELGHLSKWAIPSQIALVTEIPKTSVGKLDKKRIRLDITEWQANNSTFLSTL

Sequences:

>Translated_560_residues
MLQTRVIPPAEGAYQYPLLIKRLLMSGARYEKTREIIYRDQLRYSYPTLIERVAQLANVLTAAGVKAGDTVAVMDWDSHR
YLECMFAIPMIGAVIHTINVRLSPEQILYTMNHAEDRFVLVNSEFVGLYQAIAPHLTTVEKTLLLTDLPEKTADLPNLVG
EYEQLLAAASPQYDFLDFDENSVATTFYTTGTTGNPKGVYFTHRQLVLHTMGVSTIMGAIDSVRLLGTNDVYMPITPMFH
VHAWGLPYVATMLGLKQVYPGRYDPEFLVELWRKEKVTFSHCVPTILQMVLNAKGAQGTDFGGWKIVIGGSALNRTLYET
AKSKGIQLTAAYGMSETGPLVSCAHLNDELMAGTEDERTTYRIKAGVPGPLVEAAIVDTEGNFLPADGETQGELVLRAPW
LTEGYFNEPQKGAELWAGGWLHTGDVATLDAMGVIDIRDRIKDVIKTGGEWISSLDLEDLISRHVAVREVAVVGIADPQW
GERPFALLVVREGHAIGARELKEHLKPFVELGHLSKWAIPSQIALVTEIPKTSVGKLDKKRIRLDITEWQANNSTFLSTL
>Mature_560_residues
MLQTRVIPPAEGAYQYPLLIKRLLMSGARYEKTREIIYRDQLRYSYPTLIERVAQLANVLTAAGVKAGDTVAVMDWDSHR
YLECMFAIPMIGAVIHTINVRLSPEQILYTMNHAEDRFVLVNSEFVGLYQAIAPHLTTVEKTLLLTDLPEKTADLPNLVG
EYEQLLAAASPQYDFLDFDENSVATTFYTTGTTGNPKGVYFTHRQLVLHTMGVSTIMGAIDSVRLLGTNDVYMPITPMFH
VHAWGLPYVATMLGLKQVYPGRYDPEFLVELWRKEKVTFSHCVPTILQMVLNAKGAQGTDFGGWKIVIGGSALNRTLYET
AKSKGIQLTAAYGMSETGPLVSCAHLNDELMAGTEDERTTYRIKAGVPGPLVEAAIVDTEGNFLPADGETQGELVLRAPW
LTEGYFNEPQKGAELWAGGWLHTGDVATLDAMGVIDIRDRIKDVIKTGGEWISSLDLEDLISRHVAVREVAVVGIADPQW
GERPFALLVVREGHAIGARELKEHLKPFVELGHLSKWAIPSQIALVTEIPKTSVGKLDKKRIRLDITEWQANNSTFLSTL

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=549, Percent_Identity=26.775956284153, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI187761345, Length=552, Percent_Identity=27.1739130434783, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI187761343, Length=552, Percent_Identity=27.1739130434783, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI38505220, Length=521, Percent_Identity=26.1036468330134, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI115511026, Length=532, Percent_Identity=23.8721804511278, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI42544132, Length=524, Percent_Identity=22.9007633587786, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI58082049, Length=523, Percent_Identity=22.1797323135755, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI157311624, Length=538, Percent_Identity=22.4907063197026, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI157311622, Length=538, Percent_Identity=22.4907063197026, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI28416953, Length=542, Percent_Identity=24.169741697417, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI122937307, Length=547, Percent_Identity=23.9488117001828, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI221142682, Length=515, Percent_Identity=25.8252427184466, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI145693145, Length=518, Percent_Identity=24.9034749034749, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1788107, Length=554, Percent_Identity=25.6317689530686, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1786810, Length=533, Percent_Identity=26.4540337711069, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1790505, Length=533, Percent_Identity=25.328330206379, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788595, Length=446, Percent_Identity=23.0941704035874, Blast_Score=91, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=380, Percent_Identity=27.3684210526316, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17531443, Length=512, Percent_Identity=24.21875, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71994694, Length=545, Percent_Identity=24.5871559633028, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71994690, Length=545, Percent_Identity=24.954128440367, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=545, Percent_Identity=24.5871559633028, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17558820, Length=516, Percent_Identity=25, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=524, Percent_Identity=22.5190839694656, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17538037, Length=524, Percent_Identity=22.3282442748092, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI32563687, Length=368, Percent_Identity=27.7173913043478, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71996755, Length=538, Percent_Identity=24.1635687732342, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17560140, Length=524, Percent_Identity=22.5190839694656, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=548, Percent_Identity=25.5474452554745, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=415, Percent_Identity=22.4096385542169, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17556552, Length=349, Percent_Identity=24.3553008595989, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71982997, Length=552, Percent_Identity=24.2753623188406, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71983001, Length=552, Percent_Identity=24.2753623188406, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=377, Percent_Identity=26.2599469496021, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6323182, Length=552, Percent_Identity=24.4565217391304, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI18859661, Length=480, Percent_Identity=25.4166666666667, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21355181, Length=511, Percent_Identity=23.2876712328767, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24581924, Length=566, Percent_Identity=24.0282685512367, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24653035, Length=508, Percent_Identity=21.6535433070866, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356947, Length=361, Percent_Identity=24.6537396121884, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24656500, Length=527, Percent_Identity=23.1499051233397, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI161076582, Length=557, Percent_Identity=20.8258527827648, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648257, Length=530, Percent_Identity=21.8867924528302, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356441, Length=327, Percent_Identity=24.7706422018349, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI20130357, Length=557, Percent_Identity=21.1849192100539, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648260, Length=493, Percent_Identity=21.501014198783, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 62111; Mature: 62111

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQTRVIPPAEGAYQYPLLIKRLLMSGARYEKTREIIYRDQLRYSYPTLIERVAQLANVL
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
TAAGVKAGDTVAVMDWDSHRYLECMFAIPMIGAVIHTINVRLSPEQILYTMNHAEDRFVL
HHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEECHHHHEEEECCCCCEEEE
VNSEFVGLYQAIAPHLTTVEKTLLLTDLPEKTADLPNLVGEYEQLLAAASPQYDFLDFDE
ECCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCC
NSVATTFYTTGTTGNPKGVYFTHRQLVLHTMGVSTIMGAIDSVRLLGTNDVYMPITPMFH
CCEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEECCCCEE
VHAWGLPYVATMLGLKQVYPGRYDPEFLVELWRKEKVTFSHCVPTILQMVLNAKGAQGTD
EEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCC
FGGWKIVIGGSALNRTLYETAKSKGIQLTAAYGMSETGPLVSCAHLNDELMAGTEDERTT
CCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCCHHHCCCCCCCEE
YRIKAGVPGPLVEAAIVDTEGNFLPADGETQGELVLRAPWLTEGYFNEPQKGAELWAGGW
EEEECCCCCCCEEEEEEECCCCEECCCCCCCCCEEEECCCCCCCCCCCCCCCHHHCCCCC
LHTGDVATLDAMGVIDIRDRIKDVIKTGGEWISSLDLEDLISRHVAVREVAVVGIADPQW
EECCCEEEECCCCEEHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHEEEEEECCCCC
GERPFALLVVREGHAIGARELKEHLKPFVELGHLSKWAIPSQIALVTEIPKTSVGKLDKK
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCC
RIRLDITEWQANNSTFLSTL
EEEEEEEEECCCCCEEEECC
>Mature Secondary Structure
MLQTRVIPPAEGAYQYPLLIKRLLMSGARYEKTREIIYRDQLRYSYPTLIERVAQLANVL
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
TAAGVKAGDTVAVMDWDSHRYLECMFAIPMIGAVIHTINVRLSPEQILYTMNHAEDRFVL
HHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEECHHHHEEEECCCCCEEEE
VNSEFVGLYQAIAPHLTTVEKTLLLTDLPEKTADLPNLVGEYEQLLAAASPQYDFLDFDE
ECCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCC
NSVATTFYTTGTTGNPKGVYFTHRQLVLHTMGVSTIMGAIDSVRLLGTNDVYMPITPMFH
CCEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEECCCCEE
VHAWGLPYVATMLGLKQVYPGRYDPEFLVELWRKEKVTFSHCVPTILQMVLNAKGAQGTD
EEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCC
FGGWKIVIGGSALNRTLYETAKSKGIQLTAAYGMSETGPLVSCAHLNDELMAGTEDERTT
CCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCCHHHCCCCCCCEE
YRIKAGVPGPLVEAAIVDTEGNFLPADGETQGELVLRAPWLTEGYFNEPQKGAELWAGGW
EEEECCCCCCCEEEEEEECCCCEECCCCCCCCCEEEECCCCCCCCCCCCCCCHHHCCCCC
LHTGDVATLDAMGVIDIRDRIKDVIKTGGEWISSLDLEDLISRHVAVREVAVVGIADPQW
EECCCEEEECCCCEEHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHEEEEEECCCCC
GERPFALLVVREGHAIGARELKEHLKPFVELGHLSKWAIPSQIALVTEIPKTSVGKLDKK
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCC
RIRLDITEWQANNSTFLSTL
EEEEEEEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]