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The map label for this gene is ddl

Identifier: 77460892

GI number: 77460892

Start: 5272734

End: 5273708

Strand: Reverse

Name: ddl

Synonym: Pfl01_4671

Alternate gene names: 77460892

Gene position: 5273708-5272734 (Counterclockwise)

Preceding gene: 77460893

Following gene: 77460891

Centisome position: 81.91

GC content: 61.95

Gene sequence:

>975_bases
ATGACTGCTGCCTACGCCAATCTGTTCTCCACCATCGCCCCGAAGGATTTCGGCCGTGTGGCCGTGTTGTTCGGTGGCCT
GAGTGCCGAGCGTGAAGTCTCGCTGAAGTCCGGCAATGCCGTGCTCACTGCGCTGCAAAGCGCAGGTGTGGACGCGTTCG
GCATCGACGTCGGCGCCGACATTCTGCAGCGCCTGCTGAGCGAGAAGATCGACCGCGCGTTCATCATCCTCCACGGTCGT
GGCGGTGAAGACGGCAGCATGCAGGGCCTGCTCGAAGTGGCCGGCATTCCGTACACCGGCAGTGGCATCCTGGCTTCGGC
ACTGGCGATGGACAAGTTGCGCACCAAGCAGGTCTGGCACACGCTCGGGATTCCGACGCCGCGTCACGCCGTGCTGCGCA
GCGAGGCCGATTGTATTTCGGCGGCGACGGAACTGGGCTTCCCTTTGATCGTCAAACCGGCGCATGAAGGTTCAAGTATC
GGGATGGCCAAAGTGAGTTCTGCGTCCGAGTTGATCGACGCATGGAAAGCGGCCAGTACCTACGATTCGCAAGTGTTGGT
CGAGCAATGGATCCACGGTCCGGAGTTCACCATCGCCACCCTGCGTGACCAGGTGTTGCCTCCTATCGCCCTGGGTACGC
CGCACACGTTCTACGACTACGACGCCAAGTACATCGCCAACGATACCCAGTATCGGATTCCGTGCGGGCTCGATGCCGCC
AAGGAAAAAGAACTCATGGACCTCACGGCGCAAGCCTGCGAGGCGCTGGGTATTGCCGGTTGGGGCCGGGCGGACGTGAT
GCAGGACGCCGACGGCAAATTCTGGTTCCTGGAAGTCAACACCGCTCCCGGCATGACCGATCACAGCCTGGTACCGATGG
CGGCGAAAGCGGCCGGTCTGGATTTCCAGCAGCTGGTCCTGGCCATTCTGGCCGCCAGTGTCGATGCCGACGGCAAGAAA
GAGGCGCGAGGTTAA

Upstream 100 bases:

>100_bases
TGTGCCAGGGCGCCGGTGACATCGGCGGTCTCGCACCGAAACTGTTGAAAAGCGAGTTGTTCGCCGGTGCCGTGGCGGCA
TCGGTCGAGGGGAAGTTGAA

Downstream 100 bases:

>100_bases
AACCATGCAAGGCGCACAGCTCAGACATCAGCCACCCGCACCCGGCCGCAAGCCGGTGCCGCGGGGTGCCAGCCGAATGG
TGGCCAAAGAGCCGATGTCC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MTAAYANLFSTIAPKDFGRVAVLFGGLSAEREVSLKSGNAVLTALQSAGVDAFGIDVGADILQRLLSEKIDRAFIILHGR
GGEDGSMQGLLEVAGIPYTGSGILASALAMDKLRTKQVWHTLGIPTPRHAVLRSEADCISAATELGFPLIVKPAHEGSSI
GMAKVSSASELIDAWKAASTYDSQVLVEQWIHGPEFTIATLRDQVLPPIALGTPHTFYDYDAKYIANDTQYRIPCGLDAA
KEKELMDLTAQACEALGIAGWGRADVMQDADGKFWFLEVNTAPGMTDHSLVPMAAKAAGLDFQQLVLAILAASVDADGKK
EARG

Sequences:

>Translated_324_residues
MTAAYANLFSTIAPKDFGRVAVLFGGLSAEREVSLKSGNAVLTALQSAGVDAFGIDVGADILQRLLSEKIDRAFIILHGR
GGEDGSMQGLLEVAGIPYTGSGILASALAMDKLRTKQVWHTLGIPTPRHAVLRSEADCISAATELGFPLIVKPAHEGSSI
GMAKVSSASELIDAWKAASTYDSQVLVEQWIHGPEFTIATLRDQVLPPIALGTPHTFYDYDAKYIANDTQYRIPCGLDAA
KEKELMDLTAQACEALGIAGWGRADVMQDADGKFWFLEVNTAPGMTDHSLVPMAAKAAGLDFQQLVLAILAASVDADGKK
EARG
>Mature_323_residues
TAAYANLFSTIAPKDFGRVAVLFGGLSAEREVSLKSGNAVLTALQSAGVDAFGIDVGADILQRLLSEKIDRAFIILHGRG
GEDGSMQGLLEVAGIPYTGSGILASALAMDKLRTKQVWHTLGIPTPRHAVLRSEADCISAATELGFPLIVKPAHEGSSIG
MAKVSSASELIDAWKAASTYDSQVLVEQWIHGPEFTIATLRDQVLPPIALGTPHTFYDYDAKYIANDTQYRIPCGLDAAK
EKELMDLTAQACEALGIAGWGRADVMQDADGKFWFLEVNTAPGMTDHSLVPMAAKAAGLDFQQLVLAILAASVDADGKKE
ARG

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=299, Percent_Identity=51.505016722408, Blast_Score=314, Evalue=4e-87,
Organism=Escherichia coli, GI1786579, Length=343, Percent_Identity=32.6530612244898, Blast_Score=154, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_PSEPF (Q3K746)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_350399.1
- ProteinModelPortal:   Q3K746
- SMR:   Q3K746
- STRING:   Q3K746
- World-2DPAGE:   0008:Q3K746
- GeneID:   3714571
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_4671
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   RAFIILH
- ProtClustDB:   PRK01372
- BioCyc:   PFLU205922:PFL_4671-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 34374; Mature: 34242

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAYANLFSTIAPKDFGRVAVLFGGLSAEREVSLKSGNAVLTALQSAGVDAFGIDVGAD
CCCHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCHHHHHHHHCCCCEEECCHHHH
ILQRLLSEKIDRAFIILHGRGGEDGSMQGLLEVAGIPYTGSGILASALAMDKLRTKQVWH
HHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
TLGIPTPRHAVLRSEADCISAATELGFPLIVKPAHEGSSIGMAKVSSASELIDAWKAAST
HHCCCCCHHHHHHCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
YDSQVLVEQWIHGPEFTIATLRDQVLPPIALGTPHTFYDYDAKYIANDTQYRIPCGLDAA
CCHHHHHHHHCCCCCEEHHHHHHHCCCCEECCCCCCEECCCCCEEECCCCEEECCCCCHH
KEKELMDLTAQACEALGIAGWGRADVMQDADGKFWFLEVNTAPGMTDHSLVPMAAKAAGL
HHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHCCC
DFQQLVLAILAASVDADGKKEARG
CHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TAAYANLFSTIAPKDFGRVAVLFGGLSAEREVSLKSGNAVLTALQSAGVDAFGIDVGAD
CCHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCHHHHHHHHCCCCEEECCHHHH
ILQRLLSEKIDRAFIILHGRGGEDGSMQGLLEVAGIPYTGSGILASALAMDKLRTKQVWH
HHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
TLGIPTPRHAVLRSEADCISAATELGFPLIVKPAHEGSSIGMAKVSSASELIDAWKAAST
HHCCCCCHHHHHHCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
YDSQVLVEQWIHGPEFTIATLRDQVLPPIALGTPHTFYDYDAKYIANDTQYRIPCGLDAA
CCHHHHHHHHCCCCCEEHHHHHHHCCCCEECCCCCCEECCCCCEEECCCCEEECCCCCHH
KEKELMDLTAQACEALGIAGWGRADVMQDADGKFWFLEVNTAPGMTDHSLVPMAAKAAGL
HHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHCCC
DFQQLVLAILAASVDADGKKEARG
CHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA