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The map label for this gene is tgt [H]

Identifier: 77460842

GI number: 77460842

Start: 5219534

End: 5220667

Strand: Reverse

Name: tgt [H]

Synonym: Pfl01_4621

Alternate gene names: 77460842

Gene position: 5220667-5219534 (Counterclockwise)

Preceding gene: 77460843

Following gene: 77460841

Centisome position: 81.09

GC content: 60.58

Gene sequence:

>1134_bases
ATGAGTCGCACCTGTCGTATGTCGTTCGAGCTGCTTGCCACCGATGGCAAGGCCCGTCGTGGTCGTCTGACCTTCCCCCG
TGGCACCGTCGAGACCCCGGCCTTCATGCCGGTGGGCACCTACGGCACGGTCAAGGGCATGTTGCCGCGTGACATCGTCG
CCACCGGCGCGGAGATCATTCTGGGCAACACTTTCCACCTGTGGCTGCGCCCGGGTACCGAAGTGATCAAGGAGCACGGC
GACCTGCACGATTTCATGCAGTGGAAAGGCCCGATCCTGACCGACTCCGGCGGTTTCCAGGTGTTCAGCCTCGGCGCCAT
GCGCAAGATCAAGGAGGAGGGCGTGACCTTCGCCTCCCCGGTCGACGGCTCGAAAGTGTTCATGGGCCCGGAAGAGTCGA
TGCAGGTCCAGCGCGACCTGGGCTCGGACATCGTGATGATCTTCGACGAATGCACCCCTTACCCGGCTGACGAAGACGTG
GCCCGCGTGTCCATGGAACTGTCGCTGCGCTGGGCCCAGCGTTCGAAGAATGCCCATGGCGACAACACGGCGGCACTGTT
CGGCATCGTTCAGGGCGGCATGCACCAGGACCTGCGCATGCGCTCGCTGGAAGGCCTGGACAAGATCGGCTTCGACGGCC
TGGCCATCGGCGGTCTGTCGGTGGGCGAACCCAAGCACGAAATGATCAAGGTGCTGGACTATCTGCCGGGCATGATGCCG
GCTGACAAACCTCGTTACCTTATGGGCGTTGGCAAACCGGAAGATCTGGTTGAGGGTGTGCGCCGCGGTGTGGACATGTT
CGATTGCGTGATGCCAACCCGTAATGCCCGCAACGGGCATCTGTTCATTGATACAGGCGTGCTGAAGATCCGTAACGCGT
TCCATCGCCATGATGATTCGCCACTGGATCCGACCTGCGATTGCTATACCTGCCAGAACTTCTCCCGCGCTTATCTGCAT
CATCTGGACAAGTGCGGCGAAATGCTCGGCAGCATGCTCAATACCATCCACAATTTGCGCCATTATCAGGTGCTGATGGC
TGGTTTGCGCGAGGCTATTCAACAGGGTACATTGGCCGCCTTTGTCGATGCCTTCTACGCCAAACGCGGGCTTCCCGTTC
CGCCTTTGGACTGA

Upstream 100 bases:

>100_bases
CCTACAAAGCCGCGGTCGATAACGGATACCGCTTTTTCAGCTACGGTGATGCGATGTTCATCACCCGTAATCCCGCGCCG
CGCGGCCCAGAGGAAACAGT

Downstream 100 bases:

>100_bases
GTTTTCTGACCCCAAGATTCAACATTTGCAACTGGAGTGCTAAATGAGCTTTTTTATCTCTAATGCCATGGCTGACGCGG
CTGCACCGGCTGCAGCGCCT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSRTCRMSFELLATDGKARRGRLTFPRGTVETPAFMPVGTYGTVKGMLPRDIVATGAEIILGNTFHLWLRPGTEVIKEHG
DLHDFMQWKGPILTDSGGFQVFSLGAMRKIKEEGVTFASPVDGSKVFMGPEESMQVQRDLGSDIVMIFDECTPYPADEDV
ARVSMELSLRWAQRSKNAHGDNTAALFGIVQGGMHQDLRMRSLEGLDKIGFDGLAIGGLSVGEPKHEMIKVLDYLPGMMP
ADKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFIDTGVLKIRNAFHRHDDSPLDPTCDCYTCQNFSRAYLH
HLDKCGEMLGSMLNTIHNLRHYQVLMAGLREAIQQGTLAAFVDAFYAKRGLPVPPLD

Sequences:

>Translated_377_residues
MSRTCRMSFELLATDGKARRGRLTFPRGTVETPAFMPVGTYGTVKGMLPRDIVATGAEIILGNTFHLWLRPGTEVIKEHG
DLHDFMQWKGPILTDSGGFQVFSLGAMRKIKEEGVTFASPVDGSKVFMGPEESMQVQRDLGSDIVMIFDECTPYPADEDV
ARVSMELSLRWAQRSKNAHGDNTAALFGIVQGGMHQDLRMRSLEGLDKIGFDGLAIGGLSVGEPKHEMIKVLDYLPGMMP
ADKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFIDTGVLKIRNAFHRHDDSPLDPTCDCYTCQNFSRAYLH
HLDKCGEMLGSMLNTIHNLRHYQVLMAGLREAIQQGTLAAFVDAFYAKRGLPVPPLD
>Mature_376_residues
SRTCRMSFELLATDGKARRGRLTFPRGTVETPAFMPVGTYGTVKGMLPRDIVATGAEIILGNTFHLWLRPGTEVIKEHGD
LHDFMQWKGPILTDSGGFQVFSLGAMRKIKEEGVTFASPVDGSKVFMGPEESMQVQRDLGSDIVMIFDECTPYPADEDVA
RVSMELSLRWAQRSKNAHGDNTAALFGIVQGGMHQDLRMRSLEGLDKIGFDGLAIGGLSVGEPKHEMIKVLDYLPGMMPA
DKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFIDTGVLKIRNAFHRHDDSPLDPTCDCYTCQNFSRAYLHH
LDKCGEMLGSMLNTIHNLRHYQVLMAGLREAIQQGTLAAFVDAFYAKRGLPVPPLD

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=350, Percent_Identity=44.5714285714286, Blast_Score=294, Evalue=1e-79,
Organism=Escherichia coli, GI1786607, Length=373, Percent_Identity=69.7050938337802, Blast_Score=555, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI17544680, Length=360, Percent_Identity=41.9444444444444, Blast_Score=290, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI25146502, Length=139, Percent_Identity=28.7769784172662, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19920510, Length=362, Percent_Identity=44.7513812154696, Blast_Score=314, Evalue=6e-86,
Organism=Drosophila melanogaster, GI21355311, Length=265, Percent_Identity=24.1509433962264, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41821; Mature: 41690

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTCRMSFELLATDGKARRGRLTFPRGTVETPAFMPVGTYGTVKGMLPRDIVATGAEII
CCCCCCEEEEEEECCCCCCCCEECCCCCCCCCCCCEECCCCCCCCCCCCHHHHHCCCEEE
LGNTFHLWLRPGTEVIKEHGDLHDFMQWKGPILTDSGGFQVFSLGAMRKIKEEGVTFASP
ECCEEEEEECCCHHHHHHCCCHHHHHHCCCCEEECCCCCEEEEHHHHHHHHHCCCEEECC
VDGSKVFMGPEESMQVQRDLGSDIVMIFDECTPYPADEDVARVSMELSLRWAQRSKNAHG
CCCCEEEECCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
DNTAALFGIVQGGMHQDLRMRSLEGLDKIGFDGLAIGGLSVGEPKHEMIKVLDYLPGMMP
CCHHHHHHHHHCCCHHHHHHHHHHCHHHCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCC
ADKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFIDTGVLKIRNAFHRHDDS
CCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHCCCCC
PLDPTCDCYTCQNFSRAYLHHLDKCGEMLGSMLNTIHNLRHYQVLMAGLREAIQQGTLAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
FVDAFYAKRGLPVPPLD
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SRTCRMSFELLATDGKARRGRLTFPRGTVETPAFMPVGTYGTVKGMLPRDIVATGAEII
CCCCCEEEEEEECCCCCCCCEECCCCCCCCCCCCEECCCCCCCCCCCCHHHHHCCCEEE
LGNTFHLWLRPGTEVIKEHGDLHDFMQWKGPILTDSGGFQVFSLGAMRKIKEEGVTFASP
ECCEEEEEECCCHHHHHHCCCHHHHHHCCCCEEECCCCCEEEEHHHHHHHHHCCCEEECC
VDGSKVFMGPEESMQVQRDLGSDIVMIFDECTPYPADEDVARVSMELSLRWAQRSKNAHG
CCCCEEEECCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
DNTAALFGIVQGGMHQDLRMRSLEGLDKIGFDGLAIGGLSVGEPKHEMIKVLDYLPGMMP
CCHHHHHHHHHCCCHHHHHHHHHHCHHHCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCC
ADKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFIDTGVLKIRNAFHRHDDS
CCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHCCCCC
PLDPTCDCYTCQNFSRAYLHHLDKCGEMLGSMLNTIHNLRHYQVLMAGLREAIQQGTLAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
FVDAFYAKRGLPVPPLD
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]