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The map label for this gene is ynfL [H]

Identifier: 77460812

GI number: 77460812

Start: 5190576

End: 5191463

Strand: Direct

Name: ynfL [H]

Synonym: Pfl01_4591

Alternate gene names: 77460812

Gene position: 5190576-5191463 (Clockwise)

Preceding gene: 77460807

Following gene: 77460813

Centisome position: 80.62

GC content: 61.15

Gene sequence:

>888_bases
GTGCTTTCAACCCGTCAATTGCGTTACTTCGTGGAAATCGCCGAGAGCGGCAGCTTCAGTGCTGCAGCCGAACGGCTGTT
CATTGCTCAATCGGCGTTGAGCCGACAGATCAAGGACATGGAAACCCGCCTGCAAACGCCGCTGTTCGAACGCACCGCGC
GCCAGCCCCGACTCACCGCCGCTGGCGAAGCGTTCCTGCCCCGGGCGAAAAATCTGCTCAATGAACTGAACAAGGCCAGC
ACCCTGGCCACGGAAATCGGCCAAGGGCAGCGCGGCACCTTGCGCCTTTGTCACTCCAGCACCGTGCCGATCAGTGGCCG
ACTGTTGCGCGACATGAGCGCATATCTGGAGCAGCAGCCCGGCGTTTCGCTGGACATCGGCACGCTCTCCTCGGAGGCGC
AACTTGAAGAACTGGCCGAAGGCCGGCTCGACATCGGACTGTTGCGCCTGCCGGTGCTGCGCCAGCGCGAGGGCGTTCAG
GTTGTGCCGCTCTATAACGAACGCTTGCTGCTTGCCGTGCCGGCGGATCATCGACTGGCTTTGATGGAGGCAGTCGATCT
GGCGCAATTGAAGGACGAAGCATTCATCTCCATCCCGCATCCGCAGCGCGGCGGGCTGAGCTATTTGTGCGCCGATCTGT
GCATGCGTCACGGTTTCTTCCCCCAGGCTGCGAGGGTGATGTCGCGCAAGACCACGCAATTGCAGTTGATCCAGGCCGGA
TTCGGCATCGCTCTACTGCCGGAATCGATGCAGGACATCGCGCCCGCTGGTGTGAGATTTCTGCCGCTGACCGGCGATTG
CCAAAGCACTGTCGCCCTCGCTTCTCGGCAAAATCCCACACCTCTGGTGCAACACTTCCTCCAGACATTCACCGGCGAAT
GCCTTTAA

Upstream 100 bases:

>100_bases
TCGCTCGCTTCCTTTATAAGCCTGCGATGACTTTAGCCAGCGCCGAGGGTTGCGACTAATATCTTCGAAACCGCCAAGCC
ATCTCGAAAAGGCATGTCTC

Downstream 100 bases:

>100_bases
ACTGCGCCCCATGATTAAAGATCCCTTTGCAAGACTCGGCCTCGACCGCGAAGTCCTGACCGTCAGCCAGCTCAACGGCC
GCGCGCGGGTGTTGCTCGAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MLSTRQLRYFVEIAESGSFSAAAERLFIAQSALSRQIKDMETRLQTPLFERTARQPRLTAAGEAFLPRAKNLLNELNKAS
TLATEIGQGQRGTLRLCHSSTVPISGRLLRDMSAYLEQQPGVSLDIGTLSSEAQLEELAEGRLDIGLLRLPVLRQREGVQ
VVPLYNERLLLAVPADHRLALMEAVDLAQLKDEAFISIPHPQRGGLSYLCADLCMRHGFFPQAARVMSRKTTQLQLIQAG
FGIALLPESMQDIAPAGVRFLPLTGDCQSTVALASRQNPTPLVQHFLQTFTGECL

Sequences:

>Translated_295_residues
MLSTRQLRYFVEIAESGSFSAAAERLFIAQSALSRQIKDMETRLQTPLFERTARQPRLTAAGEAFLPRAKNLLNELNKAS
TLATEIGQGQRGTLRLCHSSTVPISGRLLRDMSAYLEQQPGVSLDIGTLSSEAQLEELAEGRLDIGLLRLPVLRQREGVQ
VVPLYNERLLLAVPADHRLALMEAVDLAQLKDEAFISIPHPQRGGLSYLCADLCMRHGFFPQAARVMSRKTTQLQLIQAG
FGIALLPESMQDIAPAGVRFLPLTGDCQSTVALASRQNPTPLVQHFLQTFTGECL
>Mature_295_residues
MLSTRQLRYFVEIAESGSFSAAAERLFIAQSALSRQIKDMETRLQTPLFERTARQPRLTAAGEAFLPRAKNLLNELNKAS
TLATEIGQGQRGTLRLCHSSTVPISGRLLRDMSAYLEQQPGVSLDIGTLSSEAQLEELAEGRLDIGLLRLPVLRQREGVQ
VVPLYNERLLLAVPADHRLALMEAVDLAQLKDEAFISIPHPQRGGLSYLCADLCMRHGFFPQAARVMSRKTTQLQLIQAG
FGIALLPESMQDIAPAGVRFLPLTGDCQSTVALASRQNPTPLVQHFLQTFTGECL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=263, Percent_Identity=33.0798479087452, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1788887, Length=277, Percent_Identity=30.3249097472924, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI2367136, Length=257, Percent_Identity=31.5175097276265, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI157672245, Length=254, Percent_Identity=32.6771653543307, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1790399, Length=288, Percent_Identity=29.1666666666667, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI145693105, Length=248, Percent_Identity=29.4354838709677, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786401, Length=273, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786508, Length=254, Percent_Identity=26.3779527559055, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787806, Length=254, Percent_Identity=25.9842519685039, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI87081904, Length=195, Percent_Identity=29.7435897435897, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790262, Length=257, Percent_Identity=27.6264591439689, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788748, Length=246, Percent_Identity=27.6422764227642, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787530, Length=188, Percent_Identity=25, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693193, Length=128, Percent_Identity=31.25, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786713, Length=95, Percent_Identity=40, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32491; Mature: 32491

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTRQLRYFVEIAESGSFSAAAERLFIAQSALSRQIKDMETRLQTPLFERTARQPRLTA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC
AGEAFLPRAKNLLNELNKASTLATEIGQGQRGTLRLCHSSTVPISGRLLRDMSAYLEQQP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCC
GVSLDIGTLSSEAQLEELAEGRLDIGLLRLPVLRQREGVQVVPLYNERLLLAVPADHRLA
CCEEEECCCCCHHHHHHHHCCCCCCHHHHCCHHHCCCCCEEEEECCCEEEEEECCCCHHH
LMEAVDLAQLKDEAFISIPHPQRGGLSYLCADLCMRHGFFPQAARVMSRKTTQLQLIQAG
HHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHC
FGIALLPESMQDIAPAGVRFLPLTGDCQSTVALASRQNPTPLVQHFLQTFTGECL
CCEEECCHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLSTRQLRYFVEIAESGSFSAAAERLFIAQSALSRQIKDMETRLQTPLFERTARQPRLTA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC
AGEAFLPRAKNLLNELNKASTLATEIGQGQRGTLRLCHSSTVPISGRLLRDMSAYLEQQP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCC
GVSLDIGTLSSEAQLEELAEGRLDIGLLRLPVLRQREGVQVVPLYNERLLLAVPADHRLA
CCEEEECCCCCHHHHHHHHCCCCCCHHHHCCHHHCCCCCEEEEECCCEEEEEECCCCHHH
LMEAVDLAQLKDEAFISIPHPQRGGLSYLCADLCMRHGFFPQAARVMSRKTTQLQLIQAG
HHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHC
FGIALLPESMQDIAPAGVRFLPLTGDCQSTVALASRQNPTPLVQHFLQTFTGECL
CCEEECCHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]