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The map label for this gene is cmoB [H]

Identifier: 77460804

GI number: 77460804

Start: 5179520

End: 5180476

Strand: Direct

Name: cmoB [H]

Synonym: Pfl01_4583

Alternate gene names: 77460804

Gene position: 5179520-5180476 (Clockwise)

Preceding gene: 77460803

Following gene: 77460807

Centisome position: 80.45

GC content: 60.71

Gene sequence:

>957_bases
ATGATTGATCTGTCCCCCCTCGCCCGCCGTCTGGCCGGCACGCCGCTGGCCGAATGGGCCAACACCCTGCAAGTGCAGCT
CGACAAAAAAATGGAGAAAGGCCACGGCGATCTGGAGCGCTGGCAAAGTGCGCTGGACGCCCTGCCGAAGATCCAGCCGA
CCGAAGTCGACCTGCTCGACGGCCTGAAGCTGGACACCAATTGCGACGACGAAACCCGCGCGCAAATGCGTACGGCGCTG
ATGGGGCTGTCGCCGTGGCGCAAGGGGCCGTTCGATCTGTTCGGCGTGCACGTCGACACCGAATGGCGCTCGGACTGGAA
GTGGTCGCGGGTCTCTCCGCATCTGGATCTGAAGGGCAAGCGCATCCTCGATGTCGGTTGTGGTAACGGCTATTACATGT
GGCGAATGCTCGGTGCCGGCGCCGACAGCGTGATCGGTGTCGATCCGAACTGGCTGTTCTTCTGCCAGTTCCAAGCGGTA
CAGCGTTATCTGTCAGAGCCGAATGCCTGGCACCTGCCGTTCCCGTTCGAAGACCTGCCGCCGAATCTGGAAGGCTTCGA
CACGGTGTTTTCGATGGGTGTGTTCTATCACCGTCGTTCGCCGATCGAGCATTTGCTGGCGCTGAAGGATTGCCTGGTGA
AGGGTGGCGAACTGGTGCTGGAGACGCTGGTGGTCGAAGGCGACAAGCATCAGGTGCTGGTGCCGGAAGATCGTTATGCG
CAGATGCGTAACGTGTGGTTCTTGCCGTCGGTGCCGGCGCTGGAATTGTGGCTGCGTCGTGCGGGGTTCACCGACGTTCG
TTGCGTGGATGTCAGCATGACAACGGTCGATGAACAGCGTGGGACCGAGTGGATGAAGTATCAGTCGCTGAGTGATTTCC
TTGATCCGGATGACCACAGCAAGACGATTGAAGGGCTGCCGGCGCCGATGCGGGCGGTCATCGTCGCGAAGAAGTAA

Upstream 100 bases:

>100_bases
ACAGCCTCGAAGAACACCGCGAGCGCCTGCTGGCCGCCGGGTTCTCGAAGGTCGTGCCGTGGTTCCAGTGTCTTAACTTT
GCCTCGTTGATTGCCTTGCC

Downstream 100 bases:

>100_bases
CGATCCCTTCCAGACACTGCAAAAAATTGTGGGAGCTGGCTTGCCAGCGATAACGGTGGATCAGTCGACATCAATGCTTG
ATGTGCCGACGCCATCGCCG

Product: methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MIDLSPLARRLAGTPLAEWANTLQVQLDKKMEKGHGDLERWQSALDALPKIQPTEVDLLDGLKLDTNCDDETRAQMRTAL
MGLSPWRKGPFDLFGVHVDTEWRSDWKWSRVSPHLDLKGKRILDVGCGNGYYMWRMLGAGADSVIGVDPNWLFFCQFQAV
QRYLSEPNAWHLPFPFEDLPPNLEGFDTVFSMGVFYHRRSPIEHLLALKDCLVKGGELVLETLVVEGDKHQVLVPEDRYA
QMRNVWFLPSVPALELWLRRAGFTDVRCVDVSMTTVDEQRGTEWMKYQSLSDFLDPDDHSKTIEGLPAPMRAVIVAKK

Sequences:

>Translated_318_residues
MIDLSPLARRLAGTPLAEWANTLQVQLDKKMEKGHGDLERWQSALDALPKIQPTEVDLLDGLKLDTNCDDETRAQMRTAL
MGLSPWRKGPFDLFGVHVDTEWRSDWKWSRVSPHLDLKGKRILDVGCGNGYYMWRMLGAGADSVIGVDPNWLFFCQFQAV
QRYLSEPNAWHLPFPFEDLPPNLEGFDTVFSMGVFYHRRSPIEHLLALKDCLVKGGELVLETLVVEGDKHQVLVPEDRYA
QMRNVWFLPSVPALELWLRRAGFTDVRCVDVSMTTVDEQRGTEWMKYQSLSDFLDPDDHSKTIEGLPAPMRAVIVAKK
>Mature_318_residues
MIDLSPLARRLAGTPLAEWANTLQVQLDKKMEKGHGDLERWQSALDALPKIQPTEVDLLDGLKLDTNCDDETRAQMRTAL
MGLSPWRKGPFDLFGVHVDTEWRSDWKWSRVSPHLDLKGKRILDVGCGNGYYMWRMLGAGADSVIGVDPNWLFFCQFQAV
QRYLSEPNAWHLPFPFEDLPPNLEGFDTVFSMGVFYHRRSPIEHLLALKDCLVKGGELVLETLVVEGDKHQVLVPEDRYA
QMRNVWFLPSVPALELWLRRAGFTDVRCVDVSMTTVDEQRGTEWMKYQSLSDFLDPDDHSKTIEGLPAPMRAVIVAKK

Specific function: Catalyzes the conversion of 5-hydroxyuridine (ho5U) to 5-methoxyuridine (mo5U) at position 34 in tRNA [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. CmoB family [H]

Homologues:

Organism=Escherichia coli, GI1788178, Length=323, Percent_Identity=54.4891640866873, Blast_Score=351, Evalue=4e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010017 [H]

Pfam domain/function: PF08003 Methyltransf_9 [H]

EC number: NA

Molecular weight: Translated: 36325; Mature: 36325

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLSPLARRLAGTPLAEWANTLQVQLDKKMEKGHGDLERWQSALDALPKIQPTEVDLLD
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHC
GLKLDTNCDDETRAQMRTALMGLSPWRKGPFDLFGVHVDTEWRSDWKWSRVSPHLDLKGK
CEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCC
RILDVGCGNGYYMWRMLGAGADSVIGVDPNWLFFCQFQAVQRYLSEPNAWHLPFPFEDLP
EEEEECCCCCEEEEEEECCCCCCEEECCCCEEEEEEHHHHHHHHCCCCCEECCCCCCCCC
PNLEGFDTVFSMGVFYHRRSPIEHLLALKDCLVKGGELVLETLVVEGDKHQVLVPEDRYA
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHEEHHHHCCCCCCEEECCCHHHH
QMRNVWFLPSVPALELWLRRAGFTDVRCVDVSMTTVDEQRGTEWMKYQSLSDFLDPDDHS
HHHCEEECCCCHHHHHHHHHCCCCCEEEEEEEEEECCHHHCCHHHHHHHHHHCCCCCCCC
KTIEGLPAPMRAVIVAKK
HHHCCCCCCCEEEEEECC
>Mature Secondary Structure
MIDLSPLARRLAGTPLAEWANTLQVQLDKKMEKGHGDLERWQSALDALPKIQPTEVDLLD
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHC
GLKLDTNCDDETRAQMRTALMGLSPWRKGPFDLFGVHVDTEWRSDWKWSRVSPHLDLKGK
CEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCC
RILDVGCGNGYYMWRMLGAGADSVIGVDPNWLFFCQFQAVQRYLSEPNAWHLPFPFEDLP
EEEEECCCCCEEEEEEECCCCCCEEECCCCEEEEEEHHHHHHHHCCCCCEECCCCCCCCC
PNLEGFDTVFSMGVFYHRRSPIEHLLALKDCLVKGGELVLETLVVEGDKHQVLVPEDRYA
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHEEHHHHCCCCCCEEECCCHHHH
QMRNVWFLPSVPALELWLRRAGFTDVRCVDVSMTTVDEQRGTEWMKYQSLSDFLDPDDHS
HHHCEEECCCCHHHHHHHHHCCCCCEEEEEEEEEECCHHHCCHHHHHHHHHHCCCCCCCC
KTIEGLPAPMRAVIVAKK
HHHCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA