Click here to switch to the map view.

The map label for this gene is glpF [H]

Identifier: 77460752

GI number: 77460752

Start: 5104775

End: 5105626

Strand: Direct

Name: glpF [H]

Synonym: Pfl01_4531

Alternate gene names: 77460752

Gene position: 5104775-5105626 (Clockwise)

Preceding gene: 77460749

Following gene: 77460753

Centisome position: 79.29

GC content: 59.86

Gene sequence:

>852_bases
ATGACAACTGCGTTACAGCAACCTTCGCTCTCGAGCCAGTGCCTGGCCGAGTTTCTGGGTACTGCACTCCTGATCTTTTT
CGGCACGGGTTGTGTCGCCGCGCTCAAGGTCGCGGGCGCCAGCTTCGGTCTCTGGGAAATCAGCATCATCTGGGGCGTCG
GCGTCAGCATGGCGATCTACCTCACCGCCGGCGTTTCCGGCGCGCACCTGAACCCTGCCGTCAGTATCGCCCTGAGCATT
TTCGCCGACTTCGAAAAGCGCAAACTGCCGTTCTACATTCTGGCCCAGGTCGCCGGCGCCTTCTGCGGCGCGCTGTTGGT
CTACACGCTGTACAGCAACCTGTTCTTCGATTACGAACAAACTCACCATATGGTTCGCGGCACTGAAGCCAGCCTCGAAC
TGGCATCGGTGTTCTCCACCTTCCCGAACCCTGCGCTGTCGACTGCGCAAGCGTTCTTGGTCGAGGTGATCATCACTGCG
ATTCTGATGGGCGTGATCATGTCCCTGACCGACGACAACAACGGCCTGCCGAAAGGCCCGCTGGCGCCACTGCTGATCGG
TCTGCTGATTGCCGTGATTGGCAGCTCGATGGGCCCGCTGACCGGTTTCGCGATGAACCCGGCACGGGACTTCGGCCCGA
AACTGATGACTTTCTTCGCCGGCTGGGGTGAAATTTCCTTCACCGGCGCACGTGAAATCCCGTACTTCCTGATTCCGATT
TTTGCACCGATTGTCGGTGCCTGCCTCGGCGCTGCCGGGTATCGCGGGCTGATTGCCCGTCACCTGACCGGCGCCACACC
TGCTACAAAGGATGCAGAACCGGCCATTGACGGCAAACCAAGAACTTCTTGA

Upstream 100 bases:

>100_bases
CAGCAGTAAACCGGGAACAGGATGGGGATCCTGCCTCGGCGAAAATCGCGCCTTGCCCTGTCGGCAACGCGCCAGACAAC
AACAAAAACCGAGGTTTTCA

Downstream 100 bases:

>100_bases
AACAGTCGGCGCGGACTCCTGCCCTGATAGAGACCGCGCCACGGCCCACTCTCCCTTATTTCGTCCAAGGCAATCGACAT
GACCGACATTGAGAATAAGA

Product: Aquaporin

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Glycerol diffusion facilitator [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTTALQQPSLSSQCLAEFLGTALLIFFGTGCVAALKVAGASFGLWEISIIWGVGVSMAIYLTAGVSGAHLNPAVSIALSI
FADFEKRKLPFYILAQVAGAFCGALLVYTLYSNLFFDYEQTHHMVRGTEASLELASVFSTFPNPALSTAQAFLVEVIITA
ILMGVIMSLTDDNNGLPKGPLAPLLIGLLIAVIGSSMGPLTGFAMNPARDFGPKLMTFFAGWGEISFTGAREIPYFLIPI
FAPIVGACLGAAGYRGLIARHLTGATPATKDAEPAIDGKPRTS

Sequences:

>Translated_283_residues
MTTALQQPSLSSQCLAEFLGTALLIFFGTGCVAALKVAGASFGLWEISIIWGVGVSMAIYLTAGVSGAHLNPAVSIALSI
FADFEKRKLPFYILAQVAGAFCGALLVYTLYSNLFFDYEQTHHMVRGTEASLELASVFSTFPNPALSTAQAFLVEVIITA
ILMGVIMSLTDDNNGLPKGPLAPLLIGLLIAVIGSSMGPLTGFAMNPARDFGPKLMTFFAGWGEISFTGAREIPYFLIPI
FAPIVGACLGAAGYRGLIARHLTGATPATKDAEPAIDGKPRTS
>Mature_282_residues
TTALQQPSLSSQCLAEFLGTALLIFFGTGCVAALKVAGASFGLWEISIIWGVGVSMAIYLTAGVSGAHLNPAVSIALSIF
ADFEKRKLPFYILAQVAGAFCGALLVYTLYSNLFFDYEQTHHMVRGTEASLELASVFSTFPNPALSTAQAFLVEVIITAI
LMGVIMSLTDDNNGLPKGPLAPLLIGLLIAVIGSSMGPLTGFAMNPARDFGPKLMTFFAGWGEISFTGAREIPYFLIPIF
APIVGACLGAAGYRGLIARHLTGATPATKDAEPAIDGKPRTS

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=257, Percent_Identity=42.0233463035019, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI22538420, Length=270, Percent_Identity=40, Blast_Score=174, Evalue=9e-44,
Organism=Homo sapiens, GI4826645, Length=267, Percent_Identity=37.8277153558052, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI4502187, Length=251, Percent_Identity=33.0677290836653, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI310133356, Length=218, Percent_Identity=30.2752293577982, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI310114181, Length=218, Percent_Identity=30.2752293577982, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI4502179, Length=284, Percent_Identity=30.6338028169014, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI6912506, Length=289, Percent_Identity=27.681660899654, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI45446752, Length=204, Percent_Identity=30.8823529411765, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI86792455, Length=213, Percent_Identity=31.924882629108, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI4502183, Length=240, Percent_Identity=31.6666666666667, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1790362, Length=278, Percent_Identity=67.6258992805755, Blast_Score=390, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71992966, Length=273, Percent_Identity=34.4322344322344, Blast_Score=144, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI71994009, Length=260, Percent_Identity=33.0769230769231, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17544068, Length=261, Percent_Identity=36.0153256704981, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32564052, Length=278, Percent_Identity=31.294964028777, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17533613, Length=278, Percent_Identity=31.294964028777, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17531429, Length=268, Percent_Identity=31.7164179104478, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17531431, Length=268, Percent_Identity=31.7164179104478, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71992961, Length=273, Percent_Identity=30.7692307692308, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71993722, Length=248, Percent_Identity=28.2258064516129, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI212646268, Length=287, Percent_Identity=25.4355400696864, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17558372, Length=274, Percent_Identity=25.9124087591241, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321054, Length=216, Percent_Identity=37.962962962963, Blast_Score=142, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6322985, Length=282, Percent_Identity=28.3687943262411, Blast_Score=112, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24652747, Length=213, Percent_Identity=32.3943661971831, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45551084, Length=213, Percent_Identity=32.3943661971831, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45550503, Length=278, Percent_Identity=28.0575539568345, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24762344, Length=260, Percent_Identity=29.6153846153846, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24762346, Length=260, Percent_Identity=29.6153846153846, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24762348, Length=260, Percent_Identity=29.6153846153846, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20130305, Length=260, Percent_Identity=29.6153846153846, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922828, Length=199, Percent_Identity=29.6482412060301, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 29733; Mature: 29602

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTALQQPSLSSQCLAEFLGTALLIFFGTGCVAALKVAGASFGLWEISIIWGVGVSMAIY
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHH
LTAGVSGAHLNPAVSIALSIFADFEKRKLPFYILAQVAGAFCGALLVYTLYSNLFFDYEQ
HCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
THHMVRGTEASLELASVFSTFPNPALSTAQAFLVEVIITAILMGVIMSLTDDNNGLPKGP
HHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LAPLLIGLLIAVIGSSMGPLTGFAMNPARDFGPKLMTFFAGWGEISFTGAREIPYFLIPI
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHH
FAPIVGACLGAAGYRGLIARHLTGATPATKDAEPAIDGKPRTS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTALQQPSLSSQCLAEFLGTALLIFFGTGCVAALKVAGASFGLWEISIIWGVGVSMAIY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHH
LTAGVSGAHLNPAVSIALSIFADFEKRKLPFYILAQVAGAFCGALLVYTLYSNLFFDYEQ
HCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
THHMVRGTEASLELASVFSTFPNPALSTAQAFLVEVIITAILMGVIMSLTDDNNGLPKGP
HHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LAPLLIGLLIAVIGSSMGPLTGFAMNPARDFGPKLMTFFAGWGEISFTGAREIPYFLIPI
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHH
FAPIVGACLGAAGYRGLIARHLTGATPATKDAEPAIDGKPRTS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9141691; 10984043 [H]