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The map label for this gene is pyrC [H]

Identifier: 77460740

GI number: 77460740

Start: 5093938

End: 5094984

Strand: Direct

Name: pyrC [H]

Synonym: Pfl01_4519

Alternate gene names: 77460740

Gene position: 5093938-5094984 (Clockwise)

Preceding gene: 77460737

Following gene: 77460741

Centisome position: 79.12

GC content: 64.09

Gene sequence:

>1047_bases
ATGTCCGACCGCCTGACCCTGCTGCGTCCCGACGACTGGCACATTCATCTTCGCGATGGTGCCGTGTTGACCAATACCGT
TGCGGATGTCGCGCGCACCTTTGGTCGCGCCATCATCATGCCCAACCTGGTACCTCCGGTGCGCAACGCCGCTGAAGCCG
ACGGCTATCGCCAGCGGATTCTCGCTGCCCGCCCGGCCGGCAGTCGTTTCGAACCGCTGATGGTGCTGTACCTCACCGAC
CGCACCCAGCCCGAAGAAATCCGTGAGGCCAAGGCCAGCGGTTTCGTGCACGCCGCCAAGCTGTATCCGGCTGGCGCCAC
CACCAACTCCGATTCCGGGGTCACCAGCATCGACAAGATTTTCCCGGTGCTGGAAGCCATGGCCGAAGCCGGCATGCCGC
TGTTGATCCACGGCGAAGTCACCCGTGGCGACGTCGACGTGTTCGACCGCGAAAAAATCTTCATCGACGAGCACATGCGC
CGCGTGGTCGAGCGTTTCCCGACACTGAAAGTGGTGTTCGAACACATCACCACCGGCGATGCCGTGCAGTTCGTCAACGA
GGCTTCGGCCAACGTCGGCGCGACCATCACCGCGCATCACCTGCTCTACAACCGCAACCACATGCTGGTGGGCGGGATCC
GGCCGCACTTCTATTGCCTGCCGATCCTCAAACGCAACACTCACCAGGAAGCCCTGCTCGACGCCGCCACCAGCGGCAGC
GCGAAGTTCTTCCTCGGTACCGACTCGGCACCGCACGCCCAGCACGCCAAGGAAGCCGCCTGCGGCTGCGCCGGCTGCTA
CACCGCGTACGCCGCCATCGAGCTGTATGCCGAAGCCTTTGAACAGCGCAACGCGCTGGACAAGCTCGAAGCCTTCGCCA
GCCTCAACGGCCCGCGTTTCTACGGTCTGCCGGCGAACACCGATCGCATCACACTGGTTCGTGACGAATGGACCGCCCCG
ACCAGCCTGCCTTTCGGCGAGCTGACCGTTATCCCGCTGCGCGCCGGTGAAAAACTGCGCTGGCGCCTGCTGGAGGAACA
CGCGTGA

Upstream 100 bases:

>100_bases
TATGGGATATCGGACGCTCGTCGCAAAACTTGATAGCGAGTGCCTGCAAGAGTCTTTTCCGGTAGCATTCGCCTCAGTTT
GACCCGCCTGGAATCCCCTC

Downstream 100 bases:

>100_bases
GTGAAGACCATTTCGACGACGAACTGGACGGTCAAGGTGGCGGCGGTTCCCGTCATCCAATGGCAGCACGCTTTCGCGGC
TACCTGCCGGTTGTCGTCGA

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MSDRLTLLRPDDWHIHLRDGAVLTNTVADVARTFGRAIIMPNLVPPVRNAAEADGYRQRILAARPAGSRFEPLMVLYLTD
RTQPEEIREAKASGFVHAAKLYPAGATTNSDSGVTSIDKIFPVLEAMAEAGMPLLIHGEVTRGDVDVFDREKIFIDEHMR
RVVERFPTLKVVFEHITTGDAVQFVNEASANVGATITAHHLLYNRNHMLVGGIRPHFYCLPILKRNTHQEALLDAATSGS
AKFFLGTDSAPHAQHAKEAACGCAGCYTAYAAIELYAEAFEQRNALDKLEAFASLNGPRFYGLPANTDRITLVRDEWTAP
TSLPFGELTVIPLRAGEKLRWRLLEEHA

Sequences:

>Translated_348_residues
MSDRLTLLRPDDWHIHLRDGAVLTNTVADVARTFGRAIIMPNLVPPVRNAAEADGYRQRILAARPAGSRFEPLMVLYLTD
RTQPEEIREAKASGFVHAAKLYPAGATTNSDSGVTSIDKIFPVLEAMAEAGMPLLIHGEVTRGDVDVFDREKIFIDEHMR
RVVERFPTLKVVFEHITTGDAVQFVNEASANVGATITAHHLLYNRNHMLVGGIRPHFYCLPILKRNTHQEALLDAATSGS
AKFFLGTDSAPHAQHAKEAACGCAGCYTAYAAIELYAEAFEQRNALDKLEAFASLNGPRFYGLPANTDRITLVRDEWTAP
TSLPFGELTVIPLRAGEKLRWRLLEEHA
>Mature_347_residues
SDRLTLLRPDDWHIHLRDGAVLTNTVADVARTFGRAIIMPNLVPPVRNAAEADGYRQRILAARPAGSRFEPLMVLYLTDR
TQPEEIREAKASGFVHAAKLYPAGATTNSDSGVTSIDKIFPVLEAMAEAGMPLLIHGEVTRGDVDVFDREKIFIDEHMRR
VVERFPTLKVVFEHITTGDAVQFVNEASANVGATITAHHLLYNRNHMLVGGIRPHFYCLPILKRNTHQEALLDAATSGSA
KFFLGTDSAPHAQHAKEAACGCAGCYTAYAAIELYAEAFEQRNALDKLEAFASLNGPRFYGLPANTDRITLVRDEWTAPT
SLPFGELTVIPLRAGEKLRWRLLEEHA

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787301, Length=342, Percent_Identity=56.140350877193, Blast_Score=401, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323452, Length=356, Percent_Identity=33.9887640449438, Blast_Score=183, Evalue=4e-47,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.2.3 [H]

Molecular weight: Translated: 38480; Mature: 38349

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00482 DIHYDROOROTASE_1 ; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRLTLLRPDDWHIHLRDGAVLTNTVADVARTFGRAIIMPNLVPPVRNAAEADGYRQRI
CCCCEEEECCCCCEEEEECCCEEHHHHHHHHHHHCCEEECCCCCCCCCCHHHCCHHHHHH
LAARPAGSRFEPLMVLYLTDRTQPEEIREAKASGFVHAAKLYPAGATTNSDSGVTSIDKI
HEECCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHH
FPVLEAMAEAGMPLLIHGEVTRGDVDVFDREKIFIDEHMRRVVERFPTLKVVFEHITTGD
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHCCCH
AVQFVNEASANVGATITAHHLLYNRNHMLVGGIRPHFYCLPILKRNTHQEALLDAATSGS
HHHHHHHCCCCCCCEEEEEHEEECCCEEEEECCCCCEEEEEHHHCCCHHHHHHHHCCCCC
AKFFLGTDSAPHAQHAKEAACGCAGCYTAYAAIELYAEAFEQRNALDKLEAFASLNGPRF
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
YGLPANTDRITLVRDEWTAPTSLPFGELTVIPLRAGEKLRWRLLEEHA
ECCCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
SDRLTLLRPDDWHIHLRDGAVLTNTVADVARTFGRAIIMPNLVPPVRNAAEADGYRQRI
CCCEEEECCCCCEEEEECCCEEHHHHHHHHHHHCCEEECCCCCCCCCCHHHCCHHHHHH
LAARPAGSRFEPLMVLYLTDRTQPEEIREAKASGFVHAAKLYPAGATTNSDSGVTSIDKI
HEECCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHH
FPVLEAMAEAGMPLLIHGEVTRGDVDVFDREKIFIDEHMRRVVERFPTLKVVFEHITTGD
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHCCCH
AVQFVNEASANVGATITAHHLLYNRNHMLVGGIRPHFYCLPILKRNTHQEALLDAATSGS
HHHHHHHCCCCCCCEEEEEHEEECCCEEEEECCCCCEEEEEHHHCCCHHHHHHHHCCCCC
AKFFLGTDSAPHAQHAKEAACGCAGCYTAYAAIELYAEAFEQRNALDKLEAFASLNGPRF
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
YGLPANTDRITLVRDEWTAPTSLPFGELTVIPLRAGEKLRWRLLEEHA
ECCCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA