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The map label for this gene is rnt [H]

Identifier: 77460741

GI number: 77460741

Start: 5094981

End: 5095655

Strand: Direct

Name: rnt [H]

Synonym: Pfl01_4520

Alternate gene names: 77460741

Gene position: 5094981-5095655 (Clockwise)

Preceding gene: 77460740

Following gene: 77460743

Centisome position: 79.13

GC content: 61.04

Gene sequence:

>675_bases
GTGAGTGAAGACCATTTCGACGACGAACTGGACGGTCAAGGTGGCGGCGGTTCCCGTCATCCAATGGCAGCACGCTTTCG
CGGCTACCTGCCGGTTGTCGTCGACGTAGAAACCGGTGGCTTCAACTCGGCCACCGACGCTCTGCTGGAGATTGCCGCGA
CTACCATCGCCATGGATGAAAAGGGTTTCGTCTATCCGGATCACACCTACTTCTTCCGCGTCGAGCCGTTCGAAGGCGCC
AACGTTGAACCGGCGGCACTGGAGTTCACCGGGATCAAGCTCGATCATCCGTTGCGCATGGCCGTCAGCGAAGAAACAGC
GCTGACCGACATCTTCCGTGGCGTGCGCAAGGCCTTGAAAGCCAACGGCTGCAAGCGGGCGATTCTGGTCGGCCACAACA
GCAGCTTCGACCTCGGCTTCCTCAATGCTGCCGTCGCGCGGCTGGACATGAAGCGCAATCCGTTTCACCCGTTCTCCAGC
TTCGACACTGCGACCCTCGCGGGTCTGGCCTACGGTCAAACCGTATTGGCCAAGGCCTGTCAGGCAGCCGACATCGACTT
CGACGGCCGTGAAGCGCACTCCGCGCGTTACGACACCGAGAAGACGGCCGAGCTGTTCTGCGGCATCGTCAACCGCTGGA
AACAGATGGGCGGCTGGGAAGACTTCGACGACTGA

Upstream 100 bases:

>100_bases
ACGAATGGACCGCCCCGACCAGCCTGCCTTTCGGCGAGCTGACCGTTATCCCGCTGCGCGCCGGTGAAAAACTGCGCTGG
CGCCTGCTGGAGGAACACGC

Downstream 100 bases:

>100_bases
GGGTCGTCGGGTTAAATCCCGCGCATGAAAAAACCGGCTCAAGAGCCGGTTTTTTTGTACCTCGACGTTGCGCCTTACAG
GGCAGCAGCGTTCTCGGTCA

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MSEDHFDDELDGQGGGGSRHPMAARFRGYLPVVVDVETGGFNSATDALLEIAATTIAMDEKGFVYPDHTYFFRVEPFEGA
NVEPAALEFTGIKLDHPLRMAVSEETALTDIFRGVRKALKANGCKRAILVGHNSSFDLGFLNAAVARLDMKRNPFHPFSS
FDTATLAGLAYGQTVLAKACQAADIDFDGREAHSARYDTEKTAELFCGIVNRWKQMGGWEDFDD

Sequences:

>Translated_224_residues
MSEDHFDDELDGQGGGGSRHPMAARFRGYLPVVVDVETGGFNSATDALLEIAATTIAMDEKGFVYPDHTYFFRVEPFEGA
NVEPAALEFTGIKLDHPLRMAVSEETALTDIFRGVRKALKANGCKRAILVGHNSSFDLGFLNAAVARLDMKRNPFHPFSS
FDTATLAGLAYGQTVLAKACQAADIDFDGREAHSARYDTEKTAELFCGIVNRWKQMGGWEDFDD
>Mature_223_residues
SEDHFDDELDGQGGGGSRHPMAARFRGYLPVVVDVETGGFNSATDALLEIAATTIAMDEKGFVYPDHTYFFRVEPFEGAN
VEPAALEFTGIKLDHPLRMAVSEETALTDIFRGVRKALKANGCKRAILVGHNSSFDLGFLNAAVARLDMKRNPFHPFSSF
DTATLAGLAYGQTVLAKACQAADIDFDGREAHSARYDTEKTAELFCGIVNRWKQMGGWEDFDD

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=195, Percent_Identity=64.6153846153846, Blast_Score=266, Evalue=7e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 24567; Mature: 24435

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDHFDDELDGQGGGGSRHPMAARFRGYLPVVVDVETGGFNSATDALLEIAATTIAMDE
CCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEECC
KGFVYPDHTYFFRVEPFEGANVEPAALEFTGIKLDHPLRMAVSEETALTDIFRGVRKALK
CCCCCCCCEEEEEECCCCCCCCCCCEEEEECEEECCCCEEHHCHHHHHHHHHHHHHHHHH
ANGCKRAILVGHNSSFDLGFLNAAVARLDMKRNPFHPFSSFDTATLAGLAYGQTVLAKAC
CCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QAADIDFDGREAHSARYDTEKTAELFCGIVNRWKQMGGWEDFDD
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SEDHFDDELDGQGGGGSRHPMAARFRGYLPVVVDVETGGFNSATDALLEIAATTIAMDE
CCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEECC
KGFVYPDHTYFFRVEPFEGANVEPAALEFTGIKLDHPLRMAVSEETALTDIFRGVRKALK
CCCCCCCCEEEEEECCCCCCCCCCCEEEEECEEECCCCEEHHCHHHHHHHHHHHHHHHHH
ANGCKRAILVGHNSSFDLGFLNAAVARLDMKRNPFHPFSSFDTATLAGLAYGQTVLAKAC
CCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QAADIDFDGREAHSARYDTEKTAELFCGIVNRWKQMGGWEDFDD
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA