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The map label for this gene is metG [H]

Identifier: 77460728

GI number: 77460728

Start: 5082624

End: 5084675

Strand: Direct

Name: metG [H]

Synonym: Pfl01_4507

Alternate gene names: 77460728

Gene position: 5082624-5084675 (Clockwise)

Preceding gene: 77460726

Following gene: 77460729

Centisome position: 78.94

GC content: 61.6

Gene sequence:

>2052_bases
ATGTCCGAACCACGCAAGATTCTCGTCACCAGCGCCCTGCCCTATGCCAACGGTTCGATCCACCTTGGCCACATGCTGGA
ATACATCCAGACCGATATGTGGGTGCGCTTCCAGAAGCATCGCGGCAATCAGTGCATTTATGTCTGCGCCGACGACGCCC
ACGGTTCGGCGATCATGCTGCGCGCGGAAAAGGAAGGCATCACCCCGGAACAACTGATCGCCAACGTCCAGGCCGAACAC
AGCGCCGACTTTGCCGAGTTCCTGGTGGACTTCGACAATTTCCACTCCACTCACGCCGAAGAAAACCGTGAGCTGTCGAG
CCAGATCTACCTGAAGCTGCGTGACGCCGGGCACATCGCCCAACGTTCGATCACCCAGTATTTCGACCCGGAAAAGAAAA
TGTTCCTGGCCGACCGCTTCATCAAGGGCACCTGCCCGAAGTGCGGCACTGAAGACCAGTACGGCGACAACTGCGAAAAA
TGCGGTGCGACCTACGCGCCGACCGATCTGAAGGATCCGAAGTCGGCCATCTCCGGCGCCACGCCGGTGCTCAAGGACTC
CCAGCACTTCTTCTTCAAGCTGCCGGACTTCCAGGAAATGCTGCAGGCCTGGACCCGCAGCGGCACCCTGCAGGACGCCG
TGGCCAACAAGATCGCCGAATGGCTGGACGCCGGCCTGCAACAGTGGGACATCTCCCGCGATGCGCCGTACTTCGGCTTC
GAGATCCCGGGCGAGCCGGGCAAGTACTTCTATGTGTGGCTGGACGCGCCGATCGGCTACATGGCCAGCTTCAAGAACCT
CTGCAACCGCACGCCGGAGCTGGACTTCGACGCGTTCTGGGGCAAGGACTCCACCGCCGAGCTGTATCACTTCATCGGCA
AGGACATCGTCAACTTCCACGCCCTGTTCTGGCCGGCGATGCTTGAAGGCGCGGGTTTCCGCAAGCCGACCGGAATCAAC
GTGCACGGCTACCTGACCGTCAACGGTCAGAAGATGTCCAAGTCCCGCGGCACCTTCATCAAGGCCCGTACCTACCTGGA
TCACCTGTCGCCGGAATACCTGCGCTACTACTACGCGGCCAAACTCGGCCGTGGCGTGGACGACCTCGACCTGAATCTCG
AAGACTTCGTGCAGAAGGTCAACTCCGACCTGGTTGGCAAAGTCGTCAACATCGCCAGCCGTTGCGCCGGTTTCATCCAG
AAGGGCAACGCCGGTCTGCTGGTGGACACCAACGCCGCACCGGAACTGACCGAAGCTTTCCTGGCCGCTGCGCCGAGCAT
CGCCGACGCCTATGAGGCCCGCGACTTCGCCCGTGCCATGCGCGAAACCATGGCGTTGGCCGACCGCGCCAACGCCTGGA
TCGCCGACAAGGCGCCATGGTCGCTGAACAAGCAGGAAGGCAAGCAGGATGAAGTCCAGGCGGTCTGCGCCACGGCGATC
AACCTGTTCCGCCAGCTGGTGATCTTCCTCAAACCGGTGCTGCCAGTGTTGGCTGCCGATGCCGAGGCGTTCCTCAACGT
CGCCCCGCTGACCTGGAACGACCACACCACCCTGCTGGCCAACCACCAGTTGAACGAATTCAAGCCGTTGATGACCCGCA
TCGACCCGGTAAAAGTGCAAGCCATGACCGACGCCTCGAAAGAAGACCTGACCGCCAGCCAGACCGACACCGGTGCCGCC
GCACCTGCCGGCAATGGCGAACTGGCCAAGGATCCGCTGTCGCCGGAAATCGACTTCGACACCTTCGCCGCCGTCGACCT
GCGCGTGGCGCTGATCGTCAAGGCCGAACACGTTGAAGGTGCGGACAAGCTGCTGCGTCTGACCCTCGACATCGGTGACG
AGCAACGCAACGTGTTTTCGGGGATCAAGAGCGCCTACCCGGATCCGTCCAAGCTCGACGGTCGCCTGACCATGATGATC
GCCAACCTCAAGCCACGGAAAATGAAGTTCGGCATCTCCGAAGGCATGGTGATGGCAGCCGGCCCTGGTGGTGAAGAAAT
CTACCTGCTGAGCCCGGACAGCGGCGCCAAGCCGGGTCAGCGCATCAAGTAA

Upstream 100 bases:

>100_bases
CCTCCGGACGGCATGTCCGGCAACGATTTGCAGAGGTGAAATATCTTTCCCGGCGCTTTATAGTGGCCGACCTCCGTTTC
ATCAAGTAGTGAAGCCCCAC

Downstream 100 bases:

>100_bases
GGTTCTGCCCTGACAGATCCCACAGGCGTGCTCCGTGCGCCTGTGGGATTTTTCATATCTGGCCCGCCCTCCACGGCTGC
CGGATAATGCCTAACCTTAA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 683; Mature: 682

Protein sequence:

>683_residues
MSEPRKILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKHRGNQCIYVCADDAHGSAIMLRAEKEGITPEQLIANVQAEH
SADFAEFLVDFDNFHSTHAEENRELSSQIYLKLRDAGHIAQRSITQYFDPEKKMFLADRFIKGTCPKCGTEDQYGDNCEK
CGATYAPTDLKDPKSAISGATPVLKDSQHFFFKLPDFQEMLQAWTRSGTLQDAVANKIAEWLDAGLQQWDISRDAPYFGF
EIPGEPGKYFYVWLDAPIGYMASFKNLCNRTPELDFDAFWGKDSTAELYHFIGKDIVNFHALFWPAMLEGAGFRKPTGIN
VHGYLTVNGQKMSKSRGTFIKARTYLDHLSPEYLRYYYAAKLGRGVDDLDLNLEDFVQKVNSDLVGKVVNIASRCAGFIQ
KGNAGLLVDTNAAPELTEAFLAAAPSIADAYEARDFARAMRETMALADRANAWIADKAPWSLNKQEGKQDEVQAVCATAI
NLFRQLVIFLKPVLPVLAADAEAFLNVAPLTWNDHTTLLANHQLNEFKPLMTRIDPVKVQAMTDASKEDLTASQTDTGAA
APAGNGELAKDPLSPEIDFDTFAAVDLRVALIVKAEHVEGADKLLRLTLDIGDEQRNVFSGIKSAYPDPSKLDGRLTMMI
ANLKPRKMKFGISEGMVMAAGPGGEEIYLLSPDSGAKPGQRIK

Sequences:

>Translated_683_residues
MSEPRKILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKHRGNQCIYVCADDAHGSAIMLRAEKEGITPEQLIANVQAEH
SADFAEFLVDFDNFHSTHAEENRELSSQIYLKLRDAGHIAQRSITQYFDPEKKMFLADRFIKGTCPKCGTEDQYGDNCEK
CGATYAPTDLKDPKSAISGATPVLKDSQHFFFKLPDFQEMLQAWTRSGTLQDAVANKIAEWLDAGLQQWDISRDAPYFGF
EIPGEPGKYFYVWLDAPIGYMASFKNLCNRTPELDFDAFWGKDSTAELYHFIGKDIVNFHALFWPAMLEGAGFRKPTGIN
VHGYLTVNGQKMSKSRGTFIKARTYLDHLSPEYLRYYYAAKLGRGVDDLDLNLEDFVQKVNSDLVGKVVNIASRCAGFIQ
KGNAGLLVDTNAAPELTEAFLAAAPSIADAYEARDFARAMRETMALADRANAWIADKAPWSLNKQEGKQDEVQAVCATAI
NLFRQLVIFLKPVLPVLAADAEAFLNVAPLTWNDHTTLLANHQLNEFKPLMTRIDPVKVQAMTDASKEDLTASQTDTGAA
APAGNGELAKDPLSPEIDFDTFAAVDLRVALIVKAEHVEGADKLLRLTLDIGDEQRNVFSGIKSAYPDPSKLDGRLTMMI
ANLKPRKMKFGISEGMVMAAGPGGEEIYLLSPDSGAKPGQRIK
>Mature_682_residues
SEPRKILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKHRGNQCIYVCADDAHGSAIMLRAEKEGITPEQLIANVQAEHS
ADFAEFLVDFDNFHSTHAEENRELSSQIYLKLRDAGHIAQRSITQYFDPEKKMFLADRFIKGTCPKCGTEDQYGDNCEKC
GATYAPTDLKDPKSAISGATPVLKDSQHFFFKLPDFQEMLQAWTRSGTLQDAVANKIAEWLDAGLQQWDISRDAPYFGFE
IPGEPGKYFYVWLDAPIGYMASFKNLCNRTPELDFDAFWGKDSTAELYHFIGKDIVNFHALFWPAMLEGAGFRKPTGINV
HGYLTVNGQKMSKSRGTFIKARTYLDHLSPEYLRYYYAAKLGRGVDDLDLNLEDFVQKVNSDLVGKVVNIASRCAGFIQK
GNAGLLVDTNAAPELTEAFLAAAPSIADAYEARDFARAMRETMALADRANAWIADKAPWSLNKQEGKQDEVQAVCATAIN
LFRQLVIFLKPVLPVLAADAEAFLNVAPLTWNDHTTLLANHQLNEFKPLMTRIDPVKVQAMTDASKEDLTASQTDTGAAA
PAGNGELAKDPLSPEIDFDTFAAVDLRVALIVKAEHVEGADKLLRLTLDIGDEQRNVFSGIKSAYPDPSKLDGRLTMMIA
NLKPRKMKFGISEGMVMAAGPGGEEIYLLSPDSGAKPGQRIK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=556, Percent_Identity=28.0575539568345, Blast_Score=245, Evalue=1e-64,
Organism=Homo sapiens, GI24308436, Length=529, Percent_Identity=26.2759924385633, Blast_Score=166, Evalue=5e-41,
Organism=Escherichia coli, GI1788432, Length=680, Percent_Identity=64.4117647058824, Blast_Score=929, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=554, Percent_Identity=29.7833935018051, Blast_Score=248, Evalue=8e-66,
Organism=Caenorhabditis elegans, GI212645096, Length=516, Percent_Identity=25, Blast_Score=152, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI71992781, Length=410, Percent_Identity=23.6585365853659, Blast_Score=113, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321704, Length=555, Percent_Identity=29.009009009009, Blast_Score=244, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6321610, Length=555, Percent_Identity=22.8828828828829, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24655311, Length=591, Percent_Identity=27.7495769881557, Blast_Score=226, Evalue=4e-59,
Organism=Drosophila melanogaster, GI21358483, Length=511, Percent_Identity=25.440313111546, Blast_Score=148, Evalue=1e-35,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 75726; Mature: 75594

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPRKILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKHRGNQCIYVCADDAHGSAIML
CCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHEEEHCCCCCEEEEEEECCCCCCEEEE
RAEKEGITPEQLIANVQAEHSADFAEFLVDFDNFHSTHAEENRELSSQIYLKLRDAGHIA
EECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHCCEEEEEECCCCHHH
QRSITQYFDPEKKMFLADRFIKGTCPKCGTEDQYGDNCEKCGATYAPTDLKDPKSAISGA
HHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHCCC
TPVLKDSQHFFFKLPDFQEMLQAWTRSGTLQDAVANKIAEWLDAGLQQWDISRDAPYFGF
CCCEECCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCE
EIPGEPGKYFYVWLDAPIGYMASFKNLCNRTPELDFDAFWGKDSTAELYHFIGKDIVNFH
ECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ALFWPAMLEGAGFRKPTGINVHGYLTVNGQKMSKSRGTFIKARTYLDHLSPEYLRYYYAA
HHHHHHHHCCCCCCCCCCCEEEEEEEECCCHHCCCCCCEEEHHHHHHHCCHHHHHHHHHH
KLGRGVDDLDLNLEDFVQKVNSDLVGKVVNIASRCAGFIQKGNAGLLVDTNAAPELTEAF
HHCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHH
LAAAPSIADAYEARDFARAMRETMALADRANAWIADKAPWSLNKQEGKQDEVQAVCATAI
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHCCCHHHHHHHHHHHH
NLFRQLVIFLKPVLPVLAADAEAFLNVAPLTWNDHTTLLANHQLNEFKPLMTRIDPVKVQ
HHHHHHHHHHHHHHHHHHCCHHHHEEECCEEECCCEEEEECCCHHHHHHHHHCCCCEEEE
AMTDASKEDLTASQTDTGAAAPAGNGELAKDPLSPEIDFDTFAAVDLRVALIVKAEHVEG
EECCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHEEEEEEEEEHHCCC
ADKLLRLTLDIGDEQRNVFSGIKSAYPDPSKLDGRLTMMIANLKPRKMKFGISEGMVMAA
HHHEEEEEECCCCHHHHHHHHHHHCCCCCHHCCCEEEEEEECCCCCCEEECCCCCEEEEE
GPGGEEIYLLSPDSGAKPGQRIK
CCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SEPRKILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKHRGNQCIYVCADDAHGSAIML
CCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHEEEHCCCCCEEEEEEECCCCCCEEEE
RAEKEGITPEQLIANVQAEHSADFAEFLVDFDNFHSTHAEENRELSSQIYLKLRDAGHIA
EECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHCCEEEEEECCCCHHH
QRSITQYFDPEKKMFLADRFIKGTCPKCGTEDQYGDNCEKCGATYAPTDLKDPKSAISGA
HHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHCCC
TPVLKDSQHFFFKLPDFQEMLQAWTRSGTLQDAVANKIAEWLDAGLQQWDISRDAPYFGF
CCCEECCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCE
EIPGEPGKYFYVWLDAPIGYMASFKNLCNRTPELDFDAFWGKDSTAELYHFIGKDIVNFH
ECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ALFWPAMLEGAGFRKPTGINVHGYLTVNGQKMSKSRGTFIKARTYLDHLSPEYLRYYYAA
HHHHHHHHCCCCCCCCCCCEEEEEEEECCCHHCCCCCCEEEHHHHHHHCCHHHHHHHHHH
KLGRGVDDLDLNLEDFVQKVNSDLVGKVVNIASRCAGFIQKGNAGLLVDTNAAPELTEAF
HHCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHH
LAAAPSIADAYEARDFARAMRETMALADRANAWIADKAPWSLNKQEGKQDEVQAVCATAI
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHCCCHHHHHHHHHHHH
NLFRQLVIFLKPVLPVLAADAEAFLNVAPLTWNDHTTLLANHQLNEFKPLMTRIDPVKVQ
HHHHHHHHHHHHHHHHHHCCHHHHEEECCEEECCCEEEEECCCHHHHHHHHHCCCCEEEE
AMTDASKEDLTASQTDTGAAAPAGNGELAKDPLSPEIDFDTFAAVDLRVALIVKAEHVEG
EECCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHEEEEEEEEEHHCCC
ADKLLRLTLDIGDEQRNVFSGIKSAYPDPSKLDGRLTMMIANLKPRKMKFGISEGMVMAA
HHHEEEEEECCCCHHHHHHHHHHHCCCCCHHCCCEEEEEEECCCCCCEEECCCCCEEEEE
GPGGEEIYLLSPDSGAKPGQRIK
CCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA