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The map label for this gene is mscS [H]

Identifier: 77460641

GI number: 77460641

Start: 4977325

End: 4978164

Strand: Reverse

Name: mscS [H]

Synonym: Pfl01_4420

Alternate gene names: 77460641

Gene position: 4978164-4977325 (Counterclockwise)

Preceding gene: 77460642

Following gene: 77460639

Centisome position: 77.32

GC content: 57.5

Gene sequence:

>840_bases
ATGGATTTGAATGCTGAGGTGGACAACCTGGTCAAGGCATCCCAGGCGTGGATTCCGATGATCATGGAATACGGCAGCCG
TGTGCTGCTGGCGGTGATCACCCTGGCCATTGGCTGGTGGCTGATCAACAAGGTCACGCAAAAGCTCGGCGGCCTGCTGG
CCCTGCGCAATGCCGACCTGGCGCTGCAAGGTTTCATCAGCAGCCTGGCCAACATCATTCTCAAGGTGCTGCTGATTGTC
AGCGTGGCGTCGATGATCGGCGTCGAGACCACTTCGTTCGTTGCGGCGATCGGTGCGGCCGGCCTGGCCATCGGTCTGGC
GCTGCAGGGCAGCCTGGCGAACTTCGCCGGCGGCGTACTGATTCTGTTGTTCCGTCCGTTCCGCATCGGTGACTGGATCG
AAGCCCAGGGTGTGGCGGGTACCGTCGACAGCATCCAGATCTTTCACACCGTGCTGCGCACCGGTGACAACAAGACCATC
ATCGTGCCAAACGGCAATCTGTCGAACGGCATCATCACCAACACCAACCGTCAGCCGACCCGCAAGGTTGTGTTCGACGT
GGGTGTGGATTACGAAGCCGATCTGCAAAAGGCCCGTCAGGTGCTGCTGGATCTGGCCAAGGATGATCGTGTTCTGCAGG
ATCCGGCACCCCAGGCCGTTATTTCGACACTGGGTGACAGTTCGATCACTGTTTCCCTGCGCGTCTGGGTTAAAACGGCG
GATTACTGGGATGTGATGTTCATGTTTAACGAACAATCCCGTGATCGTTTAAAAACTGCCGGAATTGATATCCCGTTTCC
GCAACGAGTGATTCGTGTGGTTCAGGAATCGGCAGTGTAA

Upstream 100 bases:

>100_bases
CGAGGCCCAAGCAACGGCAGAAACGATTGAGCCCTGTTCGGTTCGGTCTTTCTGCCGCTGGTTTTTACAGCCCGGGAAGC
CGGCAATCAGGAGATGAAAG

Downstream 100 bases:

>100_bases
TGATAGGTTGCTAATTAATTGCCTGACTTATCGGTCAGGCATCTATTACAAGTGACAGGCAATAAAAAAGGCCCATCCGA
AGATGGGCCTTTTTGTTTTG

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MDLNAEVDNLVKASQAWIPMIMEYGSRVLLAVITLAIGWWLINKVTQKLGGLLALRNADLALQGFISSLANIILKVLLIV
SVASMIGVETTSFVAAIGAAGLAIGLALQGSLANFAGGVLILLFRPFRIGDWIEAQGVAGTVDSIQIFHTVLRTGDNKTI
IVPNGNLSNGIITNTNRQPTRKVVFDVGVDYEADLQKARQVLLDLAKDDRVLQDPAPQAVISTLGDSSITVSLRVWVKTA
DYWDVMFMFNEQSRDRLKTAGIDIPFPQRVIRVVQESAV

Sequences:

>Translated_279_residues
MDLNAEVDNLVKASQAWIPMIMEYGSRVLLAVITLAIGWWLINKVTQKLGGLLALRNADLALQGFISSLANIILKVLLIV
SVASMIGVETTSFVAAIGAAGLAIGLALQGSLANFAGGVLILLFRPFRIGDWIEAQGVAGTVDSIQIFHTVLRTGDNKTI
IVPNGNLSNGIITNTNRQPTRKVVFDVGVDYEADLQKARQVLLDLAKDDRVLQDPAPQAVISTLGDSSITVSLRVWVKTA
DYWDVMFMFNEQSRDRLKTAGIDIPFPQRVIRVVQESAV
>Mature_279_residues
MDLNAEVDNLVKASQAWIPMIMEYGSRVLLAVITLAIGWWLINKVTQKLGGLLALRNADLALQGFISSLANIILKVLLIV
SVASMIGVETTSFVAAIGAAGLAIGLALQGSLANFAGGVLILLFRPFRIGDWIEAQGVAGTVDSIQIFHTVLRTGDNKTI
IVPNGNLSNGIITNTNRQPTRKVVFDVGVDYEADLQKARQVLLDLAKDDRVLQDPAPQAVISTLGDSSITVSLRVWVKTA
DYWDVMFMFNEQSRDRLKTAGIDIPFPQRVIRVVQESAV

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=276, Percent_Identity=36.5942028985507, Blast_Score=187, Evalue=5e-49,
Organism=Escherichia coli, GI1786670, Length=218, Percent_Identity=31.1926605504587, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787591, Length=268, Percent_Identity=25, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI2367355, Length=195, Percent_Identity=29.2307692307692, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 30268; Mature: 30268

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLNAEVDNLVKASQAWIPMIMEYGSRVLLAVITLAIGWWLINKVTQKLGGLLALRNADL
CCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCH
ALQGFISSLANIILKVLLIVSVASMIGVETTSFVAAIGAAGLAIGLALQGSLANFAGGVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHE
ILLFRPFRIGDWIEAQGVAGTVDSIQIFHTVLRTGDNKTIIVPNGNLSNGIITNTNRQPT
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCCC
RKVVFDVGVDYEADLQKARQVLLDLAKDDRVLQDPAPQAVISTLGDSSITVSLRVWVKTA
CEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEEEEEEEEEEC
DYWDVMFMFNEQSRDRLKTAGIDIPFPQRVIRVVQESAV
CCEEEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MDLNAEVDNLVKASQAWIPMIMEYGSRVLLAVITLAIGWWLINKVTQKLGGLLALRNADL
CCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCH
ALQGFISSLANIILKVLLIVSVASMIGVETTSFVAAIGAAGLAIGLALQGSLANFAGGVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHE
ILLFRPFRIGDWIEAQGVAGTVDSIQIFHTVLRTGDNKTIIVPNGNLSNGIITNTNRQPT
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCCC
RKVVFDVGVDYEADLQKARQVLLDLAKDDRVLQDPAPQAVISTLGDSSITVSLRVWVKTA
CEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEEEEEEEEEEC
DYWDVMFMFNEQSRDRLKTAGIDIPFPQRVIRVVQESAV
CCEEEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]