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The map label for this gene is tolB [H]

Identifier: 77460623

GI number: 77460623

Start: 4962580

End: 4963824

Strand: Reverse

Name: tolB [H]

Synonym: Pfl01_4402

Alternate gene names: 77460623

Gene position: 4963824-4962580 (Counterclockwise)

Preceding gene: 77460624

Following gene: 77460622

Centisome position: 77.1

GC content: 59.36

Gene sequence:

>1245_bases
ATGGCGGATGAAAAGAACATTCTGGTCACCAGCGGCAGCGATCGGGCAACGCCGATCGCCGTCGTGCCGTTCGGTTTCCA
GGGCGGTGCTGTCCTGCCGGATGACATGGCTGAAATCATTGGTAACGATTTGCGCAACTCGGGCTACTACTCGCCGATTC
CAAAGCAAAACATGATCAGCCAGCCAAGTCAGCCGAGCGAAATCATCTTCCGTGACTGGAAGGCGGTGGGCGCGCAATAC
ATGATGGTCGGCAGCATCGTGCCGGCCGGCGGCCGTCTGCAGGTGCAGTGGGCTCTGTTCAACGTCGCCACCGAACAGAA
AGTGGCTGACGGCAGTGTGTCCGGTACCACCGAACAGCTGCGTGACATGGCGCACTACATCTCCGATCAGTCGTTCGAAA
AACTGACCGGCATCAAAGGTGCATTCTCGACTCGCCTGCTGTACGTCACGGCCGAGCGTTTCTCCGAGAAGAACACCCGT
TACACCCTGCAGCGTTCGGATTATGACGGCGCCCGCGCCGTGACCCTGCTGCAATCGCGCGAGCCGATCCTGTCGCCGCG
TTTTGCACCGGACGGCAAGCGTATCGCCTACGTGTCGTTCGAACAGAAGCGTCCGCGCATCTTCATGCAGAACATCGACA
CCGGTCGCCGCGAGCAGATCACCAACTTCGAAGGCCTGAACGGCGCGCCTGCCTGGTCGCCGGACGGCAATCGCCTGGCG
TTCGTACTGTCGAAAGACGGTAACCCTGACATCTACGTGATGAACCTCGGTTCGCGTCAGATCACTCGCGTGACTGCAGG
TCCTGGCATCAACACCGAACCGTACTGGGGCAAGGATGGCTCGACCATCTACTTCACCTCCGACCGTGGCGGCAAACCGC
AGATCTACAAGACCAGCGCTGGTGGCGGCGGTGCCGAGCGTGTGACGTTCGTGGGCAACTACAACGCCAACCCGAAGCTT
TCGGCGGATGAAAAGACCCTGGTGATGATCCATCGTCAGGACGGTTTCACCAATTTCAAAGTGGCGGCTCAGGATTTGCA
GCGCGGAAGTGTAAAAATCCTCACTGATAGCACTCTGGACGAGTCGCCTACTGTTGCGCCCAACGGCACCATGGTAATCT
ACGCCACCCGCCAGCAGGGCCGGGGAGTCTTGATGCTCGTGTCCATTAATGGACGCGTGAGGCTCCCGCTTCCTACCGCT
CAAGGCGAAGTCAGAGAACCGTCCTGGTCCCCTTACCTGAACTGA

Upstream 100 bases:

>100_bases
ATCGTTCATTCAAGATGACATTCACACCTGAGGATCTAGCCTTGTGAGAAACCTTCTTCGAGGAATGCTGGTCGTGATCT
GCTGCATGGCAGGGATCGTG

Downstream 100 bases:

>100_bases
CGCGGCGCTTTACGTTTTACTTAACACACTGGGGTTCATTAGGAGTTTCACGATGGAAATGCTGAAGTTTGGTAAATTTG
CTGCGCTGGCTCTGGCCATG

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MADEKNILVTSGSDRATPIAVVPFGFQGGAVLPDDMAEIIGNDLRNSGYYSPIPKQNMISQPSQPSEIIFRDWKAVGAQY
MMVGSIVPAGGRLQVQWALFNVATEQKVADGSVSGTTEQLRDMAHYISDQSFEKLTGIKGAFSTRLLYVTAERFSEKNTR
YTLQRSDYDGARAVTLLQSREPILSPRFAPDGKRIAYVSFEQKRPRIFMQNIDTGRREQITNFEGLNGAPAWSPDGNRLA
FVLSKDGNPDIYVMNLGSRQITRVTAGPGINTEPYWGKDGSTIYFTSDRGGKPQIYKTSAGGGGAERVTFVGNYNANPKL
SADEKTLVMIHRQDGFTNFKVAAQDLQRGSVKILTDSTLDESPTVAPNGTMVIYATRQQGRGVLMLVSINGRVRLPLPTA
QGEVREPSWSPYLN

Sequences:

>Translated_414_residues
MADEKNILVTSGSDRATPIAVVPFGFQGGAVLPDDMAEIIGNDLRNSGYYSPIPKQNMISQPSQPSEIIFRDWKAVGAQY
MMVGSIVPAGGRLQVQWALFNVATEQKVADGSVSGTTEQLRDMAHYISDQSFEKLTGIKGAFSTRLLYVTAERFSEKNTR
YTLQRSDYDGARAVTLLQSREPILSPRFAPDGKRIAYVSFEQKRPRIFMQNIDTGRREQITNFEGLNGAPAWSPDGNRLA
FVLSKDGNPDIYVMNLGSRQITRVTAGPGINTEPYWGKDGSTIYFTSDRGGKPQIYKTSAGGGGAERVTFVGNYNANPKL
SADEKTLVMIHRQDGFTNFKVAAQDLQRGSVKILTDSTLDESPTVAPNGTMVIYATRQQGRGVLMLVSINGRVRLPLPTA
QGEVREPSWSPYLN
>Mature_413_residues
ADEKNILVTSGSDRATPIAVVPFGFQGGAVLPDDMAEIIGNDLRNSGYYSPIPKQNMISQPSQPSEIIFRDWKAVGAQYM
MVGSIVPAGGRLQVQWALFNVATEQKVADGSVSGTTEQLRDMAHYISDQSFEKLTGIKGAFSTRLLYVTAERFSEKNTRY
TLQRSDYDGARAVTLLQSREPILSPRFAPDGKRIAYVSFEQKRPRIFMQNIDTGRREQITNFEGLNGAPAWSPDGNRLAF
VLSKDGNPDIYVMNLGSRQITRVTAGPGINTEPYWGKDGSTIYFTSDRGGKPQIYKTSAGGGGAERVTFVGNYNANPKLS
ADEKTLVMIHRQDGFTNFKVAAQDLQRGSVKILTDSTLDESPTVAPNGTMVIYATRQQGRGVLMLVSINGRVRLPLPTAQ
GEVREPSWSPYLN

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=413, Percent_Identity=41.6464891041162, Blast_Score=318, Evalue=5e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 45482; Mature: 45351

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADEKNILVTSGSDRATPIAVVPFGFQGGAVLPDDMAEIIGNDLRNSGYYSPIPKQNMIS
CCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCC
QPSQPSEIIFRDWKAVGAQYMMVGSIVPAGGRLQVQWALFNVATEQKVADGSVSGTTEQL
CCCCCHHHHHHHHHHHCCEEEEEEEEECCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHH
RDMAHYISDQSFEKLTGIKGAFSTRLLYVTAERFSEKNTRYTLQRSDYDGARAVTLLQSR
HHHHHHHCCCCHHHHHCCCCCCCEEEEEEEEHHHCCCCCEEEEEECCCCCCEEEEEEECC
EPILSPRFAPDGKRIAYVSFEQKRPRIFMQNIDTGRREQITNFEGLNGAPAWSPDGNRLA
CCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCEEE
FVLSKDGNPDIYVMNLGSRQITRVTAGPGINTEPYWGKDGSTIYFTSDRGGKPQIYKTSA
EEEECCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECC
GGGGAERVTFVGNYNANPKLSADEKTLVMIHRQDGFTNFKVAAQDLQRGSVKILTDSTLD
CCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCEEEEEHHHHCCCCEEEEECCCCC
ESPTVAPNGTMVIYATRQQGRGVLMLVSINGRVRLPLPTAQGEVREPSWSPYLN
CCCCCCCCCEEEEEEECCCCCCEEEEEEECCEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADEKNILVTSGSDRATPIAVVPFGFQGGAVLPDDMAEIIGNDLRNSGYYSPIPKQNMIS
CCCCCEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCC
QPSQPSEIIFRDWKAVGAQYMMVGSIVPAGGRLQVQWALFNVATEQKVADGSVSGTTEQL
CCCCCHHHHHHHHHHHCCEEEEEEEEECCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHH
RDMAHYISDQSFEKLTGIKGAFSTRLLYVTAERFSEKNTRYTLQRSDYDGARAVTLLQSR
HHHHHHHCCCCHHHHHCCCCCCCEEEEEEEEHHHCCCCCEEEEEECCCCCCEEEEEEECC
EPILSPRFAPDGKRIAYVSFEQKRPRIFMQNIDTGRREQITNFEGLNGAPAWSPDGNRLA
CCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCEEE
FVLSKDGNPDIYVMNLGSRQITRVTAGPGINTEPYWGKDGSTIYFTSDRGGKPQIYKTSA
EEEECCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECC
GGGGAERVTFVGNYNANPKLSADEKTLVMIHRQDGFTNFKVAAQDLQRGSVKILTDSTLD
CCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCEEEEEHHHHCCCCEEEEECCCCC
ESPTVAPNGTMVIYATRQQGRGVLMLVSINGRVRLPLPTAQGEVREPSWSPYLN
CCCCCCCCCEEEEEEECCCCCCEEEEEEECCEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA