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The map label for this gene is gcvH [H]

Identifier: 77460612

GI number: 77460612

Start: 4950964

End: 4951347

Strand: Direct

Name: gcvH [H]

Synonym: Pfl01_4391

Alternate gene names: 77460612

Gene position: 4950964-4951347 (Clockwise)

Preceding gene: 77460611

Following gene: 77460613

Centisome position: 76.9

GC content: 61.2

Gene sequence:

>384_bases
ATGAGCGAGTTGCGTTTTACTGAAGATCACGAATGGCTGCGCGCCGAAGCCGATGGTTCTGTCACCGTTGGCATCACCGC
TTTCGCGCAGAACGCCCTGGGCGACGTGGTGTTCGTGCAACTGCCTGAATTGCAGGCTTACGAGAAAGGCGCCGAAGCCG
CCACCGTGGAATCGGTAAAAGCCGCCAGCGGCGTTTACATGCCACTGGACGGTGAAGTCCTGGCCACCAACCCGGCCCTG
GAAGACAGCCCTGAACTGGTGAACGAAGATCCGCTGGGCGAAGGCTGGTTCTTCCGCTTCAAGCCAGCCGACGCGACCGC
CGTCGCCAAGCTGCTGGATCAGGACGCCTACGACCGCCTGATCAAAGCCCAAGCCGAAGCCTGA

Upstream 100 bases:

>100_bases
TTGGCCGCGTTCTTGCTAAGTAACCGTTCATAAAATCAGGGCATTGCCGCCCGAGTATTCCACCGCGTCCACCAGACGAG
TCTGGCCCCTAGGAGATTCC

Downstream 100 bases:

>100_bases
GGAACCTGACATGACCCAAGTAAATCTCGGCACTGCCAACGAATTCATCGCCCGTCACATCGGCCCGCGCGCCGGTGACG
AGCAAGCCATGCTCAACAGC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 126

Protein sequence:

>127_residues
MSELRFTEDHEWLRAEADGSVTVGITAFAQNALGDVVFVQLPELQAYEKGAEAATVESVKAASGVYMPLDGEVLATNPAL
EDSPELVNEDPLGEGWFFRFKPADATAVAKLLDQDAYDRLIKAQAEA

Sequences:

>Translated_127_residues
MSELRFTEDHEWLRAEADGSVTVGITAFAQNALGDVVFVQLPELQAYEKGAEAATVESVKAASGVYMPLDGEVLATNPAL
EDSPELVNEDPLGEGWFFRFKPADATAVAKLLDQDAYDRLIKAQAEA
>Mature_126_residues
SELRFTEDHEWLRAEADGSVTVGITAFAQNALGDVVFVQLPELQAYEKGAEAATVESVKAASGVYMPLDGEVLATNPALE
DSPELVNEDPLGEGWFFRFKPADATAVAKLLDQDAYDRLIKAQAEA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=127, Percent_Identity=43.3070866141732, Blast_Score=102, Evalue=1e-22,
Organism=Homo sapiens, GI49574537, Length=127, Percent_Identity=43.3070866141732, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1789271, Length=122, Percent_Identity=52.4590163934426, Blast_Score=131, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17551294, Length=124, Percent_Identity=43.5483870967742, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17507493, Length=118, Percent_Identity=43.2203389830509, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319272, Length=122, Percent_Identity=37.7049180327869, Blast_Score=99, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17865652, Length=125, Percent_Identity=38.4, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13731; Mature: 13600

Theoretical pI: Translated: 3.89; Mature: 3.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELRFTEDHEWLRAEADGSVTVGITAFAQNALGDVVFVQLPELQAYEKGAEAATVESVK
CCCCCCCCCCCEEEECCCCCEEEEHHHHHHHCCCCEEEEECCCCHHHHCCCCHHHHHHHH
AASGVYMPLDGEVLATNPALEDSPELVNEDPLGEGWFFRFKPADATAVAKLLDQDAYDRL
HCCCEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCHHHHHH
IKAQAEA
HHHHCCC
>Mature Secondary Structure 
SELRFTEDHEWLRAEADGSVTVGITAFAQNALGDVVFVQLPELQAYEKGAEAATVESVK
CCCCCCCCCCEEEECCCCCEEEEHHHHHHHCCCCEEEEECCCCHHHHCCCCHHHHHHHH
AASGVYMPLDGEVLATNPALEDSPELVNEDPLGEGWFFRFKPADATAVAKLLDQDAYDRL
HCCCEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCHHHHHH
IKAQAEA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]