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The map label for this gene is relA [H]

Identifier: 77460438

GI number: 77460438

Start: 4758406

End: 4760649

Strand: Reverse

Name: relA [H]

Synonym: Pfl01_4217

Alternate gene names: 77460438

Gene position: 4760649-4758406 (Counterclockwise)

Preceding gene: 77460439

Following gene: 77460437

Centisome position: 73.94

GC content: 63.19

Gene sequence:

>2244_bases
ATGGTACAGGTGAGAGCACACCAGCCGATCAACACTGACGGCAGTATCAATCTCGAGGCTTGGCTCGATCATGCGGTCAG
TGTCGATCTGGCACTGGATCGCGAAGCCTTGAAAGAGGCCTGCGAGTACGCTCGCCAGGCCGAGCAACAGTCCAATGCAG
CGAAGAATCTCTGGTCCGAAGGCAGCGGCAGTTTCAGCACTGGCCTCGAGATCGCCGAGATCCTTGCCGACCTCAAGCTC
GATCAGGATTCGCTGGTGGCTGCGGTTCTCTATCGCGGCGTGCGCGAAGGCCAGATCGAACTCGCGGCGGTCAGCCAGCG
CTTCGGCCCGGTGGTCGCCAAGCTGATCGACGGCGTGCTGCGCATGGCGGCAATCAGTGCCAGCCTCAGCCCCCGCCAGT
CGATGGTGCTCGGCACCCAGGGCCAGGTGGAAAACCTGCGCAAGATGCTGGTGGCGATGGTCGACGACGTGCGTGTCGCG
CTGATCAAGCTCGCCGAACGCACCTGCGCGATCCGTGCAGTGAAAACCGCCGACGACGAAAAACGTAACCGGGTCGCCCG
GGAAGTGTTCGACATCTATGCGCCGCTCGCGCATCGACTCGGCATCGGCCATATCAAATGGGAACTGGAGGACTTGTCCT
TCCGTTACCTGGAACCCGATCAATACAAACAGATCGCCAAGCTGCTGCATGAACGGCGGCTGGATCGTGAGCGTTTCATC
GCCGATGTGATGACCCAGCTCAAGGACGAGCTGCAAGCCACCGGCGTCGAAGCCGACATCAGCGGTCGGGCCAAACACAT
CTATTCGATCTGGCGCAAAATGCAGCGCAAGGGTCTGGAATTCAGCCAGATCTATGACGTGCGTGCGGTGCGCGTGCTGG
TGCCGGAAATGCGCGACTGCTACACCGCGCTCGGCATCGTCCACACCCTGTGGCGGCATATCCCGAAAGAGTTCGACGAC
TACATCGCCAACCCGAAAGAGAACGGCTACCGCTCGCTGCACACGGCGGTGATCGGCCCGGAAGGCAAGGTGCTGGAAGT
CCAGATCCGGACCCATTCGATGCACGAAGAGGCCGAGCTCGGCGTCTGCGCGCACTGGCGCTACAAGGGCACCGACGTCA
AGTCCGGCTCCAATCACTACGAAGAGAAAATCTCCTGGCTGCGTCAGGTCCTCGAGTGGCACGAAGAGCTGGGCGACATC
GGTGGTCTGGCCGAACAGTTGCGGGTCGATATCGAGCCGGATCGGGTCTACATCTTCACCCCGGACGGTCACGCCATCGA
CTTGCCGAAGGGCGCGACGCCGCTGGATTTCGCCTACCGCGTGCACACCGAAATCGGTCACAACTGCCGTGGCGCGAAGA
TCAACGGGCGCATCGTTCCGCTCAACTACAGCCTGCAGACCGGCGAGCAGGTCGAGATCATCACCAGCAAGCACGGCACC
CCAAGCCGCGACTGGCTGAACCCGAACCTGGGCTACGTCACCACGTCCCGGGCGCGGGCGAAGATCGTGCACTGGTTCAA
GCTGCAGGCACGCGACCAGAACGTCGCGGCGGGCAAGACCCTGCTCGAGCGCGAACTCAATCGCCTCGGCCTGCCGGCGG
TGGATTTCGACAAGCTGGCCGACAAGGCCAACATGAAAACCGCCGAAGACATGTTCGCCGCCCTCGGGGCCGGCGACCTG
CGTCTGGCGCAACTGGTCAACCTGGCGCAACAGCTGGTCGAGCCGGAACGCGGCAACGAACAGCTGGAACTGATCCCGCG
CAAGGCCACCGGCTACAAGCCGGGCAAGCGCGGCGACATCCAGATCCAGGGCGTCGGCAACCTGATGACCCAGATGGCCG
GCTGCTGCCAGCCGTTGCCGGGCGATGCGATCGTCGGCTACATCACCCAGGGCCGTGGCGTGAGCATTCACCGTCAGGAC
TGCGCCTCGGTGCTGCAACTGGCCGGGCGCGAGCCGGAGCGGATCATCCAGGTCAGCTGGGGGCCGGTGCCGGTGCTCAC
CTATCCGGTGGACATCGTCATCCGCGCCTACGACCGTTCCGGTCTGCTGCGTGACGTTTCGCAGGTGCTGCTCAACGAGC
GGATCAACGTGCTGGCGGTCAACACCCGCTCGAACAAGGAGGACAACACCGCGCTGATGTCCCTGACCATCGAGATTCCG
GGTCTGGACGCGCTGGGGCGGTTGCTGGGGCGGATTTCCCAGTTGCCGAACATCATCGAAACGCGGCGTAACCGGACTCC
ATGA

Upstream 100 bases:

>100_bases
GGTCGTCCGTAGCGCTCGCCTTGGCCCCGCGAGCGCGAACGGGCATTGACGGTCAGTGATTTGACGCATTGAATCTGCGT
CGTAGGGAAGGTAAAGCAAG

Downstream 100 bases:

>100_bases
AACCGATCTACAGCCTTGAAGACCTGCTGCACCTGATGAACCGTCTGCGCGACCCGCAGTATGGTTGCCCGTGGGACATC
AAGCAGACTTACGCGACCAT

Product: (p)ppGpp synthetase SpoT/RelA

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 747; Mature: 747

Protein sequence:

>747_residues
MVQVRAHQPINTDGSINLEAWLDHAVSVDLALDREALKEACEYARQAEQQSNAAKNLWSEGSGSFSTGLEIAEILADLKL
DQDSLVAAVLYRGVREGQIELAAVSQRFGPVVAKLIDGVLRMAAISASLSPRQSMVLGTQGQVENLRKMLVAMVDDVRVA
LIKLAERTCAIRAVKTADDEKRNRVAREVFDIYAPLAHRLGIGHIKWELEDLSFRYLEPDQYKQIAKLLHERRLDRERFI
ADVMTQLKDELQATGVEADISGRAKHIYSIWRKMQRKGLEFSQIYDVRAVRVLVPEMRDCYTALGIVHTLWRHIPKEFDD
YIANPKENGYRSLHTAVIGPEGKVLEVQIRTHSMHEEAELGVCAHWRYKGTDVKSGSNHYEEKISWLRQVLEWHEELGDI
GGLAEQLRVDIEPDRVYIFTPDGHAIDLPKGATPLDFAYRVHTEIGHNCRGAKINGRIVPLNYSLQTGEQVEIITSKHGT
PSRDWLNPNLGYVTTSRARAKIVHWFKLQARDQNVAAGKTLLERELNRLGLPAVDFDKLADKANMKTAEDMFAALGAGDL
RLAQLVNLAQQLVEPERGNEQLELIPRKATGYKPGKRGDIQIQGVGNLMTQMAGCCQPLPGDAIVGYITQGRGVSIHRQD
CASVLQLAGREPERIIQVSWGPVPVLTYPVDIVIRAYDRSGLLRDVSQVLLNERINVLAVNTRSNKEDNTALMSLTIEIP
GLDALGRLLGRISQLPNIIETRRNRTP

Sequences:

>Translated_747_residues
MVQVRAHQPINTDGSINLEAWLDHAVSVDLALDREALKEACEYARQAEQQSNAAKNLWSEGSGSFSTGLEIAEILADLKL
DQDSLVAAVLYRGVREGQIELAAVSQRFGPVVAKLIDGVLRMAAISASLSPRQSMVLGTQGQVENLRKMLVAMVDDVRVA
LIKLAERTCAIRAVKTADDEKRNRVAREVFDIYAPLAHRLGIGHIKWELEDLSFRYLEPDQYKQIAKLLHERRLDRERFI
ADVMTQLKDELQATGVEADISGRAKHIYSIWRKMQRKGLEFSQIYDVRAVRVLVPEMRDCYTALGIVHTLWRHIPKEFDD
YIANPKENGYRSLHTAVIGPEGKVLEVQIRTHSMHEEAELGVCAHWRYKGTDVKSGSNHYEEKISWLRQVLEWHEELGDI
GGLAEQLRVDIEPDRVYIFTPDGHAIDLPKGATPLDFAYRVHTEIGHNCRGAKINGRIVPLNYSLQTGEQVEIITSKHGT
PSRDWLNPNLGYVTTSRARAKIVHWFKLQARDQNVAAGKTLLERELNRLGLPAVDFDKLADKANMKTAEDMFAALGAGDL
RLAQLVNLAQQLVEPERGNEQLELIPRKATGYKPGKRGDIQIQGVGNLMTQMAGCCQPLPGDAIVGYITQGRGVSIHRQD
CASVLQLAGREPERIIQVSWGPVPVLTYPVDIVIRAYDRSGLLRDVSQVLLNERINVLAVNTRSNKEDNTALMSLTIEIP
GLDALGRLLGRISQLPNIIETRRNRTP
>Mature_747_residues
MVQVRAHQPINTDGSINLEAWLDHAVSVDLALDREALKEACEYARQAEQQSNAAKNLWSEGSGSFSTGLEIAEILADLKL
DQDSLVAAVLYRGVREGQIELAAVSQRFGPVVAKLIDGVLRMAAISASLSPRQSMVLGTQGQVENLRKMLVAMVDDVRVA
LIKLAERTCAIRAVKTADDEKRNRVAREVFDIYAPLAHRLGIGHIKWELEDLSFRYLEPDQYKQIAKLLHERRLDRERFI
ADVMTQLKDELQATGVEADISGRAKHIYSIWRKMQRKGLEFSQIYDVRAVRVLVPEMRDCYTALGIVHTLWRHIPKEFDD
YIANPKENGYRSLHTAVIGPEGKVLEVQIRTHSMHEEAELGVCAHWRYKGTDVKSGSNHYEEKISWLRQVLEWHEELGDI
GGLAEQLRVDIEPDRVYIFTPDGHAIDLPKGATPLDFAYRVHTEIGHNCRGAKINGRIVPLNYSLQTGEQVEIITSKHGT
PSRDWLNPNLGYVTTSRARAKIVHWFKLQARDQNVAAGKTLLERELNRLGLPAVDFDKLADKANMKTAEDMFAALGAGDL
RLAQLVNLAQQLVEPERGNEQLELIPRKATGYKPGKRGDIQIQGVGNLMTQMAGCCQPLPGDAIVGYITQGRGVSIHRQD
CASVLQLAGREPERIIQVSWGPVPVLTYPVDIVIRAYDRSGLLRDVSQVLLNERINVLAVNTRSNKEDNTALMSLTIEIP
GLDALGRLLGRISQLPNIIETRRNRTP

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1789147, Length=757, Percent_Identity=47.9524438573316, Blast_Score=716, Evalue=0.0,
Organism=Escherichia coli, GI1790082, Length=696, Percent_Identity=35.9195402298851, Blast_Score=444, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 83659; Mature: 83659

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQVRAHQPINTDGSINLEAWLDHAVSVDLALDREALKEACEYARQAEQQSNAAKNLWSE
CEEEECCCCCCCCCCEEHHHHHHHHHEEEHEECHHHHHHHHHHHHHHHHHHHHHHHHHCC
GSGSFSTGLEIAEILADLKLDQDSLVAAVLYRGVREGQIELAAVSQRFGPVVAKLIDGVL
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
RMAAISASLSPRQSMVLGTQGQVENLRKMLVAMVDDVRVALIKLAERTCAIRAVKTADDE
HHHHHHCCCCCCHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHH
KRNRVAREVFDIYAPLAHRLGIGHIKWELEDLSFRYLEPDQYKQIAKLLHERRLDRERFI
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEECCCHHHHHHHHHHHHHHCCHHHHH
ADVMTQLKDELQATGVEADISGRAKHIYSIWRKMQRKGLEFSQIYDVRAVRVLVPEMRDC
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
YTALGIVHTLWRHIPKEFDDYIANPKENGYRSLHTAVIGPEGKVLEVQIRTHSMHEEAEL
HHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHEEECCCCCEEEEEEECCCCCHHHCC
GVCAHWRYKGTDVKSGSNHYEEKISWLRQVLEWHEELGDIGGLAEQLRVDIEPDRVYIFT
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEECCCCEEEEEC
PDGHAIDLPKGATPLDFAYRVHTEIGHNCRGAKINGRIVPLNYSLQTGEQVEIITSKHGT
CCCCEEECCCCCCCHHHHEEEHHHHCCCCCCCEECCEEEEEECEECCCCEEEEEECCCCC
PSRDWLNPNLGYVTTSRARAKIVHWFKLQARDQNVAAGKTLLERELNRLGLPAVDFDKLA
CCCCCCCCCCCEEECCHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHH
DKANMKTAEDMFAALGAGDLRLAQLVNLAQQLVEPERGNEQLELIPRKATGYKPGKRGDI
HHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCE
QIQGVGNLMTQMAGCCQPLPGDAIVGYITQGRGVSIHRQDCASVLQLAGREPERIIQVSW
EEEEHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHCCCCCCEEEEEC
GPVPVLTYPVDIVIRAYDRSGLLRDVSQVLLNERINVLAVNTRSNKEDNTALMSLTIEIP
CCEEEEEECCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEECC
GLDALGRLLGRISQLPNIIETRRNRTP
CHHHHHHHHHHHHHCCHHHHHHCCCCC
>Mature Secondary Structure
MVQVRAHQPINTDGSINLEAWLDHAVSVDLALDREALKEACEYARQAEQQSNAAKNLWSE
CEEEECCCCCCCCCCEEHHHHHHHHHEEEHEECHHHHHHHHHHHHHHHHHHHHHHHHHCC
GSGSFSTGLEIAEILADLKLDQDSLVAAVLYRGVREGQIELAAVSQRFGPVVAKLIDGVL
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
RMAAISASLSPRQSMVLGTQGQVENLRKMLVAMVDDVRVALIKLAERTCAIRAVKTADDE
HHHHHHCCCCCCHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHH
KRNRVAREVFDIYAPLAHRLGIGHIKWELEDLSFRYLEPDQYKQIAKLLHERRLDRERFI
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEECCCHHHHHHHHHHHHHHCCHHHHH
ADVMTQLKDELQATGVEADISGRAKHIYSIWRKMQRKGLEFSQIYDVRAVRVLVPEMRDC
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
YTALGIVHTLWRHIPKEFDDYIANPKENGYRSLHTAVIGPEGKVLEVQIRTHSMHEEAEL
HHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHEEECCCCCEEEEEEECCCCCHHHCC
GVCAHWRYKGTDVKSGSNHYEEKISWLRQVLEWHEELGDIGGLAEQLRVDIEPDRVYIFT
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEECCCCEEEEEC
PDGHAIDLPKGATPLDFAYRVHTEIGHNCRGAKINGRIVPLNYSLQTGEQVEIITSKHGT
CCCCEEECCCCCCCHHHHEEEHHHHCCCCCCCEECCEEEEEECEECCCCEEEEEECCCCC
PSRDWLNPNLGYVTTSRARAKIVHWFKLQARDQNVAAGKTLLERELNRLGLPAVDFDKLA
CCCCCCCCCCCEEECCHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHH
DKANMKTAEDMFAALGAGDLRLAQLVNLAQQLVEPERGNEQLELIPRKATGYKPGKRGDI
HHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCE
QIQGVGNLMTQMAGCCQPLPGDAIVGYITQGRGVSIHRQDCASVLQLAGREPERIIQVSW
EEEEHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHCCCCCCEEEEEC
GPVPVLTYPVDIVIRAYDRSGLLRDVSQVLLNERINVLAVNTRSNKEDNTALMSLTIEIP
CCEEEEEECCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEECC
GLDALGRLLGRISQLPNIIETRRNRTP
CHHHHHHHHHHHHHCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]