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The map label for this gene is rumA

Identifier: 77460439

GI number: 77460439

Start: 4760738

End: 4762123

Strand: Reverse

Name: rumA

Synonym: Pfl01_4218

Alternate gene names: 77460439

Gene position: 4762123-4760738 (Counterclockwise)

Preceding gene: 77460440

Following gene: 77460438

Centisome position: 73.96

GC content: 62.34

Gene sequence:

>1386_bases
ATGGCCAAGCACGAGAGAGGCCTGCGCTTCCAGCCCACCGGCGGCAGCAAGGCCCCGCAAATCCCGACCGGCAAAAAGCA
GCGCCTGAGCATCGAGCGCCTGGCCAATGACGGTCGCGGCATCGCGTTTTTTGAAGGCAAAACCTGGTTCGTCCTCGGCG
CCCTCGCCGGTGAAGAGGTCGAAGCGCGGGTGCTAGGCGCCCACGGCAAAGTGGTCGAGGCGCGTACCGAGCGCGTGTTC
AGCGCCAGTGAATTGCGTCGTCCCGCACCTTGCCAGCACGCCGGCCGCTGCGGCGGTTGCAGCGTCCAGCATTTGCCCCA
CAACGAACAGCTTGCCCTGAAACAGCGCATGCTCGCCGAGCAGTTGTCGCGAGTCGCCGGAGCCGAACCCGAGGAGTGGG
CGGCGCCGTTGACCGGTCCCGAATTCGGCTACCGTCGCCGCGCCCGGATCGCCGTGCGCTGGGACAGCAAGGCCAAAAAG
CTTGAAGTCGGTTTCCGTGCCGCCGGCAGCCAGGACATCGTTGCGATCAGCGAATGCCCGGTGCTGGTACAGCCCTTGCA
GCCGATCATGACCCGCTTGCCGGAGATGCTGCGTCGCTTGAGCAAACCTCAGGCGCTGGGGCATGTGGAATTGTTCAGTG
GCTCGTCACTCGCCGTCCTGCTGCGTCACATGGCGCCGCTGTCCGAAGCCGACCTGACGATTCTCAAGGATTTCTGCGCG
TTCCATGAAGCGCAGCTCTGGCTGCATGGCGAAGGCGAACCGCAGCCGGTGGATGATGCACAGTCGCTTGGCTATCACCT
GGAACAGTGGGATCTGCAATTGGCTTACCGGCCGGGGGATTTCATCCAGGTCAATGCCGGGGTCAACGAGGCCATGGTCG
CCCAGGCGCTGGAGTGGTTGAAACCGACAGCGGATGAGCGCGTGCTCGACCTGTTCTGCGGTCTCGGCAACTTTGCCTTG
CCGCTGGCAAAAGCTGCGCGTGAAGTGGTGGCGGTCGAAGGCGTGCAGACCATGGTTGATCGCGCGGCGGCCAATGCCGC
CAGTAACAATTTGCATAACACAAAGTTTTTTCAGGCCGATTTATCCCAGCCTTTGACCGATGCCGAGTGGATCGGAAACG
GCTTTTCTGCGGTACTCTTGGACCCACCGCGCGACGGTGCTTTCGAGGTGGTGCGCAAGCTGGCGGCCTTGGGCGCCAAA
CGGTTGGTGTACGTGTCGTGCAACCCTGCAACTCTGGCGCGCGATACGGTCGAATTGATCAAGCAGGGCTACCGGTTAAA
ACGTGCCGGGATTCTCGATATGTTTCCTCAGACGGCACATGTCGAGGCCATGGCGTTATTTGAAGCGAGCCAGGATGGCT
CGTCTGATCCGACTGGTCGTCCGTAG

Upstream 100 bases:

>100_bases
TGCGCCTGTCCAAAGAAGTTGAAAACGCGGTGATCGTTGCGATCATCTGCGACCGTGGCGACCGTTACCTGTCGACCGGC
ATTTTCGACGCGCCCAACTG

Downstream 100 bases:

>100_bases
CGCTCGCCTTGGCCCCGCGAGCGCGAACGGGCATTGACGGTCAGTGATTTGACGCATTGAATCTGCGTCGTAGGGAAGGT
AAAGCAAGATGGTACAGGTG

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MAKHERGLRFQPTGGSKAPQIPTGKKQRLSIERLANDGRGIAFFEGKTWFVLGALAGEEVEARVLGAHGKVVEARTERVF
SASELRRPAPCQHAGRCGGCSVQHLPHNEQLALKQRMLAEQLSRVAGAEPEEWAAPLTGPEFGYRRRARIAVRWDSKAKK
LEVGFRAAGSQDIVAISECPVLVQPLQPIMTRLPEMLRRLSKPQALGHVELFSGSSLAVLLRHMAPLSEADLTILKDFCA
FHEAQLWLHGEGEPQPVDDAQSLGYHLEQWDLQLAYRPGDFIQVNAGVNEAMVAQALEWLKPTADERVLDLFCGLGNFAL
PLAKAAREVVAVEGVQTMVDRAAANAASNNLHNTKFFQADLSQPLTDAEWIGNGFSAVLLDPPRDGAFEVVRKLAALGAK
RLVYVSCNPATLARDTVELIKQGYRLKRAGILDMFPQTAHVEAMALFEASQDGSSDPTGRP

Sequences:

>Translated_461_residues
MAKHERGLRFQPTGGSKAPQIPTGKKQRLSIERLANDGRGIAFFEGKTWFVLGALAGEEVEARVLGAHGKVVEARTERVF
SASELRRPAPCQHAGRCGGCSVQHLPHNEQLALKQRMLAEQLSRVAGAEPEEWAAPLTGPEFGYRRRARIAVRWDSKAKK
LEVGFRAAGSQDIVAISECPVLVQPLQPIMTRLPEMLRRLSKPQALGHVELFSGSSLAVLLRHMAPLSEADLTILKDFCA
FHEAQLWLHGEGEPQPVDDAQSLGYHLEQWDLQLAYRPGDFIQVNAGVNEAMVAQALEWLKPTADERVLDLFCGLGNFAL
PLAKAAREVVAVEGVQTMVDRAAANAASNNLHNTKFFQADLSQPLTDAEWIGNGFSAVLLDPPRDGAFEVVRKLAALGAK
RLVYVSCNPATLARDTVELIKQGYRLKRAGILDMFPQTAHVEAMALFEASQDGSSDPTGRP
>Mature_460_residues
AKHERGLRFQPTGGSKAPQIPTGKKQRLSIERLANDGRGIAFFEGKTWFVLGALAGEEVEARVLGAHGKVVEARTERVFS
ASELRRPAPCQHAGRCGGCSVQHLPHNEQLALKQRMLAEQLSRVAGAEPEEWAAPLTGPEFGYRRRARIAVRWDSKAKKL
EVGFRAAGSQDIVAISECPVLVQPLQPIMTRLPEMLRRLSKPQALGHVELFSGSSLAVLLRHMAPLSEADLTILKDFCAF
HEAQLWLHGEGEPQPVDDAQSLGYHLEQWDLQLAYRPGDFIQVNAGVNEAMVAQALEWLKPTADERVLDLFCGLGNFALP
LAKAAREVVAVEGVQTMVDRAAANAASNNLHNTKFFQADLSQPLTDAEWIGNGFSAVLLDPPRDGAFEVVRKLAALGAKR
LVYVSCNPATLARDTVELIKQGYRLKRAGILDMFPQTAHVEAMALFEASQDGSSDPTGRP

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=198, Percent_Identity=31.8181818181818, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI51173878, Length=198, Percent_Identity=31.8181818181818, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1789148, Length=420, Percent_Identity=42.1428571428571, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1787083, Length=383, Percent_Identity=28.9817232375979, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI1790403, Length=371, Percent_Identity=23.989218328841, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24666579, Length=482, Percent_Identity=24.6887966804979, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_PSEPF (Q3K8E9)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_349946.1
- ProteinModelPortal:   Q3K8E9
- SMR:   Q3K8E9
- STRING:   Q3K8E9
- GeneID:   3717718
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_4218
- eggNOG:   COG2265
- HOGENOM:   HBG690163
- OMA:   AWRPGDF
- ProtClustDB:   PRK13168
- BioCyc:   PFLU205922:PFL_4218-MONOMER
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 50407; Mature: 50276

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 407-407 BINDING 283-283 BINDING 312-312 BINDING 317-317 BINDING 333-333 BINDING 360-360 BINDING 381-381

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHERGLRFQPTGGSKAPQIPTGKKQRLSIERLANDGRGIAFFEGKTWFVLGALAGEEV
CCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCCE
EARVLGAHGKVVEARTERVFSASELRRPAPCQHAGRCGGCSVQHLPHNEQLALKQRMLAE
EEEEECCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
QLSRVAGAEPEEWAAPLTGPEFGYRRRARIAVRWDSKAKKLEVGFRAAGSQDIVAISECP
HHHHHHCCCHHHHCCCCCCCCCCCCCEEEEEEEECCCCCEEEECEECCCCCCEEEECCCC
VLVQPLQPIMTRLPEMLRRLSKPQALGHVELFSGSSLAVLLRHMAPLSEADLTILKDFCA
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHH
FHEAQLWLHGEGEPQPVDDAQSLGYHLEQWDLQLAYRPGDFIQVNAGVNEAMVAQALEWL
HHHCEEEEECCCCCCCCCHHHHHCCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHH
KPTADERVLDLFCGLGNFALPLAKAAREVVAVEGVQTMVDRAAANAASNNLHNTKFFQAD
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHH
LSQPLTDAEWIGNGFSAVLLDPPRDGAFEVVRKLAALGAKRLVYVSCNPATLARDTVELI
HCCCCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
KQGYRLKRAGILDMFPQTAHVEAMALFEASQDGSSDPTGRP
HCCCHHHHCCHHHHCCCCHHHHHHHEEECCCCCCCCCCCCC
>Mature Secondary Structure 
AKHERGLRFQPTGGSKAPQIPTGKKQRLSIERLANDGRGIAFFEGKTWFVLGALAGEEV
CCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCCE
EARVLGAHGKVVEARTERVFSASELRRPAPCQHAGRCGGCSVQHLPHNEQLALKQRMLAE
EEEEECCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
QLSRVAGAEPEEWAAPLTGPEFGYRRRARIAVRWDSKAKKLEVGFRAAGSQDIVAISECP
HHHHHHCCCHHHHCCCCCCCCCCCCCEEEEEEEECCCCCEEEECEECCCCCCEEEECCCC
VLVQPLQPIMTRLPEMLRRLSKPQALGHVELFSGSSLAVLLRHMAPLSEADLTILKDFCA
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHH
FHEAQLWLHGEGEPQPVDDAQSLGYHLEQWDLQLAYRPGDFIQVNAGVNEAMVAQALEWL
HHHCEEEEECCCCCCCCCHHHHHCCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHH
KPTADERVLDLFCGLGNFALPLAKAAREVVAVEGVQTMVDRAAANAASNNLHNTKFFQAD
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHH
LSQPLTDAEWIGNGFSAVLLDPPRDGAFEVVRKLAALGAKRLVYVSCNPATLARDTVELI
HCCCCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
KQGYRLKRAGILDMFPQTAHVEAMALFEASQDGSSDPTGRP
HCCCHHHHCCHHHHCCCCHHHHHHHEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA