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The map label for this gene is mazG [H]

Identifier: 77460437

GI number: 77460437

Start: 4757567

End: 4758409

Strand: Reverse

Name: mazG [H]

Synonym: Pfl01_4216

Alternate gene names: 77460437

Gene position: 4758409-4757567 (Counterclockwise)

Preceding gene: 77460438

Following gene: 77460436

Centisome position: 73.91

GC content: 60.38

Gene sequence:

>843_bases
ATGAAACCGATCTACAGCCTTGAAGACCTGCTGCACCTGATGAACCGTCTGCGCGACCCGCAGTATGGTTGCCCGTGGGA
CATCAAGCAGACTTACGCGACCATCGTCCCGCACACCCTTGAAGAGGCCTACGAAGTCGCCGATGCCATCGAGCGCGGCG
ACTTCGACCACTTGCAGGGGGAGTTGGGCGATCTGCTGTTTCAGGTGGTGTATTACAGCCAGCTGGCGCGGGAAGAGGGG
CGGTTCGAATTTGCCGGGGTGGTCGATAGCATCACGCGCAAGCTGATTCGTCGTCATCCGCACGTATTCCCGACCGGCGA
TCTGTATGCGCCGCTGGACGTGCCACGTTTGAACGAAGAGCAGGTCAAGCAGCGCTGGGAGGAGATCAAGGCCGAAGAAC
GTGCCGAGAAGTCCGCCGAGCCGCAGCAACTGTCACTGCTCGACGATGTGCCGGCTGCGTTGCCGGCACTGTCCCGCTCG
GCCAAGTTGCAGAAGCGCGCAGGGCAGGTCGGTTTCGACTGGCCGGATGCGTTGCCGGTGCTGGACAAAGTCCGTGAAGA
GCTCGATGAAGTGCTCGAAGCCATGTCCGAAAATGACCCGCAAGCGGTGGCGGACGAGATCGGCGACCTGCTGTTTTCGG
TGGTCAATCTGGCCCGGCACCTGAAGGTCGATCCGGAAACTGCATTGCGTGGCGCCAACGCCAAGTTCGAGCGACGTTTC
CGTTTTATCGAACAGGCATTGCGCGACACCCATCGACCCATGGAAGATTGCACCCTCGAAGAGTTGGACGCCCTGTGGGG
CGAAGCCAAACGTCAGGAAAAGAATTTGCCCAGCTGCGGCTGA

Upstream 100 bases:

>100_bases
CCCTGACCATCGAGATTCCGGGTCTGGACGCGCTGGGGCGGTTGCTGGGGCGGATTTCCCAGTTGCCGAACATCATCGAA
ACGCGGCGTAACCGGACTCC

Downstream 100 bases:

>100_bases
GCCGTTGCCCAAGTGAGTAAGCATCAATGAGCCTTTCCCTTCGCGACCAGTTGCTCAAAGCAGGGCTGGTCAACCAAAAG
CAGGCCAAGCAGGTCAGCAA

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKPIYSLEDLLHLMNRLRDPQYGCPWDIKQTYATIVPHTLEEAYEVADAIERGDFDHLQGELGDLLFQVVYYSQLAREEG
RFEFAGVVDSITRKLIRRHPHVFPTGDLYAPLDVPRLNEEQVKQRWEEIKAEERAEKSAEPQQLSLLDDVPAALPALSRS
AKLQKRAGQVGFDWPDALPVLDKVREELDEVLEAMSENDPQAVADEIGDLLFSVVNLARHLKVDPETALRGANAKFERRF
RFIEQALRDTHRPMEDCTLEELDALWGEAKRQEKNLPSCG

Sequences:

>Translated_280_residues
MKPIYSLEDLLHLMNRLRDPQYGCPWDIKQTYATIVPHTLEEAYEVADAIERGDFDHLQGELGDLLFQVVYYSQLAREEG
RFEFAGVVDSITRKLIRRHPHVFPTGDLYAPLDVPRLNEEQVKQRWEEIKAEERAEKSAEPQQLSLLDDVPAALPALSRS
AKLQKRAGQVGFDWPDALPVLDKVREELDEVLEAMSENDPQAVADEIGDLLFSVVNLARHLKVDPETALRGANAKFERRF
RFIEQALRDTHRPMEDCTLEELDALWGEAKRQEKNLPSCG
>Mature_280_residues
MKPIYSLEDLLHLMNRLRDPQYGCPWDIKQTYATIVPHTLEEAYEVADAIERGDFDHLQGELGDLLFQVVYYSQLAREEG
RFEFAGVVDSITRKLIRRHPHVFPTGDLYAPLDVPRLNEEQVKQRWEEIKAEERAEKSAEPQQLSLLDDVPAALPALSRS
AKLQKRAGQVGFDWPDALPVLDKVREELDEVLEAMSENDPQAVADEIGDLLFSVVNLARHLKVDPETALRGANAKFERRF
RFIEQALRDTHRPMEDCTLEELDALWGEAKRQEKNLPSCG

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=272, Percent_Identity=52.5735294117647, Blast_Score=272, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 32086; Mature: 32086

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIYSLEDLLHLMNRLRDPQYGCPWDIKQTYATIVPHTLEEAYEVADAIERGDFDHLQG
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ELGDLLFQVVYYSQLAREEGRFEFAGVVDSITRKLIRRHPHVFPTGDLYAPLDVPRLNEE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
QVKQRWEEIKAEERAEKSAEPQQLSLLDDVPAALPALSRSAKLQKRAGQVGFDWPDALPV
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LDKVREELDEVLEAMSENDPQAVADEIGDLLFSVVNLARHLKVDPETALRGANAKFERRF
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHH
RFIEQALRDTHRPMEDCTLEELDALWGEAKRQEKNLPSCG
HHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKPIYSLEDLLHLMNRLRDPQYGCPWDIKQTYATIVPHTLEEAYEVADAIERGDFDHLQG
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ELGDLLFQVVYYSQLAREEGRFEFAGVVDSITRKLIRRHPHVFPTGDLYAPLDVPRLNEE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
QVKQRWEEIKAEERAEKSAEPQQLSLLDDVPAALPALSRSAKLQKRAGQVGFDWPDALPV
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LDKVREELDEVLEAMSENDPQAVADEIGDLLFSVVNLARHLKVDPETALRGANAKFERRF
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHH
RFIEQALRDTHRPMEDCTLEELDALWGEAKRQEKNLPSCG
HHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]