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The map label for this gene is barA [H]

Identifier: 77460432

GI number: 77460432

Start: 4752537

End: 4754438

Strand: Direct

Name: barA [H]

Synonym: Pfl01_4211

Alternate gene names: 77460432

Gene position: 4752537-4754438 (Clockwise)

Preceding gene: 77460431

Following gene: 77460433

Centisome position: 73.82

GC content: 61.46

Gene sequence:

>1902_bases
ATGATTTTCCGCCGTTGGGACATCAACACCCGCACCCAGTTGATCAGCCTGGGCCCTGCGCTGTTGCTGACCTTGCTGCT
GATCAGCTTTTTCACCTTTGTGCGCATTCAGGATTTGCGTCAGGAGCTGAACCACACCGGCCAACTGATCGCCAACCAGC
TCGCACCGGCCACCGAGTACGGGGTGATTTCCGGCAACAACGAAGTGCTGGAAAGCCTGCTCAAGGCCACGCTGGCCACG
CCGAACGTGCGGTTTCTGGAAGTGCAGGACAGCGCCAATCGGATTCTCTCTTACGTCGAACAGCCGGCGGATGCGCACAA
TCGTCCGCATCAGGTCGAAGTGTTCCAGGCGCCGGTGCGGCTGCAACGCATCGCCTTGCACAACGATTTCTTTCAGGACG
GCAAGAGCAGCCCCGGCGTGGCCAGCGAGGACTATCTGGGACGGGTGATCGTCGGTCTGTCGAATGACGCGTTCAGTCAG
CGCCAACAGGAAATTTTGCTCAAGGCCGGGATTCTCGCGTTGTTCGCCCTGCTCTTCACGTTCGTCCTGGCGCGGCGGCT
GGCCGGCAGTCTGTCGCAGCCGATTCGCGACATCGGCAATGCGGTCAAGGCGATTCAGGACGGCGATTACAAGACCCCAC
TGCCGATTGTAGACGACACCGAACTGGGCGCCCTGTCCCAGCACATCAACAACCTGGCCCAGGCGCTGGAACAGGCCAGC
CGCGAGCAGCATCAGGCGATGGCGCAACTGATCCAGACCCGCGAAGAGGCCGAGAAAGCCAACAACGCGAAATCGGATTT
CCTGGCGATGATGAGCCATGAACTGCGCACCCCGATGAACGGTGTGCTGGGCATGCTGCAATTGCTCGAAACCACCGACA
TGACTGAGGAGCAGGTCGAGTACGCGGCGCTGGCTTCCGAGTCCACCGAGCATTTGCTCAAGGTGATCAACGACATTCTG
GATTTCTCGCGCATCGAGCGTTCGGAACTGGAGCTGGAGCACATTCCGTTCAACCTCGCCGAAGTGATCGGCGCCTGCGC
CCAGTCGTTCCAGCACAGCGCGGCGCAACGCGGGTTGGCCCTGCAGCTGCGAATCCCCGAAGACTTGCGCGACTTGCAGG
TCCAGGGCGATCCGACGCGGATCCGGCAGATTCTGGTCAACCTGGTCGGCAATGCCTTGAAGTTCACCGAACAGGGACGC
GTCAGCATCGAGGCGCAGTGGCAGTCGCTGGATCACGAGTTGCTGTGGTTCACCTGTGCGGTGCGTGACAGTGGCATCGG
TATTTCGCCGGAGAGCCTGGAGCTGATGTTCAACGCGTTCCAGCAGGCTGACAGTTCGATTTCCCGTCGTTATGGCGGCA
CCGGCCTGGGCCTGCCGATTGCCCGCACGCTCGCCGAACGGATGGGCGGCACCTTGCGCGCCCAGAGTGAAGAAGGTCGC
GGCTCGGTGTTCACCCTGGAAATACCACTGGCCCTATATAAGCAGGCGCTGCCCGCGCTTGCCGCACCGCGCACATTGAA
CGGTGCAAGTCACGGCGAAGGCCGAAATGTGCTGCTGGTCGAGGACAACCCGGTCAACCAGACGGTGATCGAAGCGATGC
TGCGCAGCCTGGGCTTTACCGTCAGCGTCGCCACCGATGGCGTGCAGGCGGTGCGCAGTGCCGGGGACAACCGTTTCGAA
GCGATTCTGATGGATTGCCGCTTGCCGCTGATCGACGGTTACGAAGCGACCCGGCGGATCCGGCAGCTGCCCGGCTGCGG
CCAGGTGCCGATCATCGCCCTGACCGCCAATGCTTTACAGGGCGACCGGGAAAACTGCCTCGCGGCGGGCATGAACGATT
ACCTGGCCAAGCCATTCAAACACAATGATCTGCAGCAGATTCTGCAGCGATGGGTGCAGTAA

Upstream 100 bases:

>100_bases
TTAAAGTCTCAAGTAATGCGCAGGTGGCTCGTTCCTTAGGGATAGAACCGATCAATGAAGCGTCTGTCGCCGCAGAGCTG
GCCGAAGGAGAGCGCCGCCC

Downstream 100 bases:

>100_bases
CAGGGCCCTTTCGAGCATCTGCGACTGGCGTGAAAAGCGAAAGTGCGGCAGTCTTAGGCACCCGAACGAGCCTCAAAAGG
GGCTTGATTAAAAATTTCAG

Product: periplasmic sensor hybrid histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MIFRRWDINTRTQLISLGPALLLTLLLISFFTFVRIQDLRQELNHTGQLIANQLAPATEYGVISGNNEVLESLLKATLAT
PNVRFLEVQDSANRILSYVEQPADAHNRPHQVEVFQAPVRLQRIALHNDFFQDGKSSPGVASEDYLGRVIVGLSNDAFSQ
RQQEILLKAGILALFALLFTFVLARRLAGSLSQPIRDIGNAVKAIQDGDYKTPLPIVDDTELGALSQHINNLAQALEQAS
REQHQAMAQLIQTREEAEKANNAKSDFLAMMSHELRTPMNGVLGMLQLLETTDMTEEQVEYAALASESTEHLLKVINDIL
DFSRIERSELELEHIPFNLAEVIGACAQSFQHSAAQRGLALQLRIPEDLRDLQVQGDPTRIRQILVNLVGNALKFTEQGR
VSIEAQWQSLDHELLWFTCAVRDSGIGISPESLELMFNAFQQADSSISRRYGGTGLGLPIARTLAERMGGTLRAQSEEGR
GSVFTLEIPLALYKQALPALAAPRTLNGASHGEGRNVLLVEDNPVNQTVIEAMLRSLGFTVSVATDGVQAVRSAGDNRFE
AILMDCRLPLIDGYEATRRIRQLPGCGQVPIIALTANALQGDRENCLAAGMNDYLAKPFKHNDLQQILQRWVQ

Sequences:

>Translated_633_residues
MIFRRWDINTRTQLISLGPALLLTLLLISFFTFVRIQDLRQELNHTGQLIANQLAPATEYGVISGNNEVLESLLKATLAT
PNVRFLEVQDSANRILSYVEQPADAHNRPHQVEVFQAPVRLQRIALHNDFFQDGKSSPGVASEDYLGRVIVGLSNDAFSQ
RQQEILLKAGILALFALLFTFVLARRLAGSLSQPIRDIGNAVKAIQDGDYKTPLPIVDDTELGALSQHINNLAQALEQAS
REQHQAMAQLIQTREEAEKANNAKSDFLAMMSHELRTPMNGVLGMLQLLETTDMTEEQVEYAALASESTEHLLKVINDIL
DFSRIERSELELEHIPFNLAEVIGACAQSFQHSAAQRGLALQLRIPEDLRDLQVQGDPTRIRQILVNLVGNALKFTEQGR
VSIEAQWQSLDHELLWFTCAVRDSGIGISPESLELMFNAFQQADSSISRRYGGTGLGLPIARTLAERMGGTLRAQSEEGR
GSVFTLEIPLALYKQALPALAAPRTLNGASHGEGRNVLLVEDNPVNQTVIEAMLRSLGFTVSVATDGVQAVRSAGDNRFE
AILMDCRLPLIDGYEATRRIRQLPGCGQVPIIALTANALQGDRENCLAAGMNDYLAKPFKHNDLQQILQRWVQ
>Mature_633_residues
MIFRRWDINTRTQLISLGPALLLTLLLISFFTFVRIQDLRQELNHTGQLIANQLAPATEYGVISGNNEVLESLLKATLAT
PNVRFLEVQDSANRILSYVEQPADAHNRPHQVEVFQAPVRLQRIALHNDFFQDGKSSPGVASEDYLGRVIVGLSNDAFSQ
RQQEILLKAGILALFALLFTFVLARRLAGSLSQPIRDIGNAVKAIQDGDYKTPLPIVDDTELGALSQHINNLAQALEQAS
REQHQAMAQLIQTREEAEKANNAKSDFLAMMSHELRTPMNGVLGMLQLLETTDMTEEQVEYAALASESTEHLLKVINDIL
DFSRIERSELELEHIPFNLAEVIGACAQSFQHSAAQRGLALQLRIPEDLRDLQVQGDPTRIRQILVNLVGNALKFTEQGR
VSIEAQWQSLDHELLWFTCAVRDSGIGISPESLELMFNAFQQADSSISRRYGGTGLGLPIARTLAERMGGTLRAQSEEGR
GSVFTLEIPLALYKQALPALAAPRTLNGASHGEGRNVLLVEDNPVNQTVIEAMLRSLGFTVSVATDGVQAVRSAGDNRFE
AILMDCRLPLIDGYEATRRIRQLPGCGQVPIIALTANALQGDRENCLAAGMNDYLAKPFKHNDLQQILQRWVQ

Specific function: Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. Phosphorylates UvrY, probably via a four-step phosphorelay [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=518, Percent_Identity=33.7837837837838, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI87081816, Length=496, Percent_Identity=32.6612903225806, Blast_Score=212, Evalue=6e-56,
Organism=Escherichia coli, GI48994928, Length=385, Percent_Identity=34.8051948051948, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1788713, Length=372, Percent_Identity=35.4838709677419, Blast_Score=205, Evalue=8e-54,
Organism=Escherichia coli, GI145693157, Length=256, Percent_Identity=37.109375, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1788393, Length=299, Percent_Identity=28.4280936454849, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1786783, Length=238, Percent_Identity=29.8319327731092, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786912, Length=262, Percent_Identity=28.6259541984733, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786600, Length=248, Percent_Identity=27.0161290322581, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790436, Length=249, Percent_Identity=26.5060240963855, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI87082128, Length=338, Percent_Identity=24.8520710059172, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790346, Length=283, Percent_Identity=31.095406360424, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1790551, Length=214, Percent_Identity=31.7757009345794, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1788549, Length=228, Percent_Identity=25, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787894, Length=228, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788279, Length=324, Percent_Identity=22.5308641975309, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789808, Length=332, Percent_Identity=23.4939759036145, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI87082012, Length=109, Percent_Identity=33.0275229357798, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1790437, Length=115, Percent_Identity=29.5652173913043, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=121, Percent_Identity=40.495867768595, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6322044, Length=123, Percent_Identity=39.8373983739837, Blast_Score=81, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323034, Length=142, Percent_Identity=32.3943661971831, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR019247
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF09984 DUF2222; PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 70014; Mature: 70014

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50885 HAMP ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFRRWDINTRTQLISLGPALLLTLLLISFFTFVRIQDLRQELNHTGQLIANQLAPATEY
CCEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GVISGNNEVLESLLKATLATPNVRFLEVQDSANRILSYVEQPADAHNRPHQVEVFQAPVR
CEECCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCHHHCCCCCEEHHHHHHHH
LQRIALHNDFFQDGKSSPGVASEDYLGRVIVGLSNDAFSQRQQEILLKAGILALFALLFT
HHHHHHHHHHHHCCCCCCCCCCHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
FVLARRLAGSLSQPIRDIGNAVKAIQDGDYKTPLPIVDDTELGALSQHINNLAQALEQAS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
REQHQAMAQLIQTREEAEKANNAKSDFLAMMSHELRTPMNGVLGMLQLLETTDMTEEQVE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHH
YAALASESTEHLLKVINDILDFSRIERSELELEHIPFNLAEVIGACAQSFQHSAAQRGLA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCE
LQLRIPEDLRDLQVQGDPTRIRQILVNLVGNALKFTEQGRVSIEAQWQSLDHELLWFTCA
EEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCEEEEEEEE
VRDSGIGISPESLELMFNAFQQADSSISRRYGGTGLGLPIARTLAERMGGTLRAQSEEGR
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCCCC
GSVFTLEIPLALYKQALPALAAPRTLNGASHGEGRNVLLVEDNPVNQTVIEAMLRSLGFT
CCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCE
VSVATDGVQAVRSAGDNRFEAILMDCRLPLIDGYEATRRIRQLPGCGQVPIIALTANALQ
EEECHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECHHHC
GDRENCLAAGMNDYLAKPFKHNDLQQILQRWVQ
CCHHHHHHHCCHHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MIFRRWDINTRTQLISLGPALLLTLLLISFFTFVRIQDLRQELNHTGQLIANQLAPATEY
CCEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GVISGNNEVLESLLKATLATPNVRFLEVQDSANRILSYVEQPADAHNRPHQVEVFQAPVR
CEECCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCHHHCCCCCEEHHHHHHHH
LQRIALHNDFFQDGKSSPGVASEDYLGRVIVGLSNDAFSQRQQEILLKAGILALFALLFT
HHHHHHHHHHHHCCCCCCCCCCHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
FVLARRLAGSLSQPIRDIGNAVKAIQDGDYKTPLPIVDDTELGALSQHINNLAQALEQAS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
REQHQAMAQLIQTREEAEKANNAKSDFLAMMSHELRTPMNGVLGMLQLLETTDMTEEQVE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHH
YAALASESTEHLLKVINDILDFSRIERSELELEHIPFNLAEVIGACAQSFQHSAAQRGLA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCE
LQLRIPEDLRDLQVQGDPTRIRQILVNLVGNALKFTEQGRVSIEAQWQSLDHELLWFTCA
EEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCEEEEEEEE
VRDSGIGISPESLELMFNAFQQADSSISRRYGGTGLGLPIARTLAERMGGTLRAQSEEGR
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCCCC
GSVFTLEIPLALYKQALPALAAPRTLNGASHGEGRNVLLVEDNPVNQTVIEAMLRSLGFT
CCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCE
VSVATDGVQAVRSAGDNRFEAILMDCRLPLIDGYEATRRIRQLPGCGQVPIIALTANALQ
EEECHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECHHHC
GDRENCLAAGMNDYLAKPFKHNDLQQILQRWVQ
CCHHHHHHHCCHHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]