Click here to switch to the map view.

The map label for this gene is 77460423

Identifier: 77460423

GI number: 77460423

Start: 4743459

End: 4744313

Strand: Reverse

Name: 77460423

Synonym: Pfl01_4202

Alternate gene names: NA

Gene position: 4744313-4743459 (Counterclockwise)

Preceding gene: 77460424

Following gene: 77460422

Centisome position: 73.69

GC content: 65.38

Gene sequence:

>855_bases
ATGAACCCCGCCGTCGAAGAAGTGCGCCTGAGCCTGCCGCATATCGAACTGGCGGCGCACCTGTTCGGCCCGGAAGACGG
GTTGCCGGTGATCGCCCTGCACGGCTGGCTGGATAACGCCAACAGCTTCGCGCGCTTGGCGCCGAAGCTCAAAGGCTTGC
GCATCGTCGCGCTGGACATGGCCGGTCACGGGCATTCCGGGCACCGGCCGAACGGCGCCGGTTACGCGCTGTGGGATTAC
GCCCATGACGTGCTGCAGGTCGCCGAGCAACTCGGCTGGAAACGTTTCGGCCTGCTCGGGCATTCCATGGGCGCAATCGT
GTCGCTGGTGCTGGCCGGGTCGTTGCCCGAACGCATCACCCATCTGGCGTTGATCGACGGGGTAATTCCTCCTACCGACA
AAGGCGAGAACGCCGCCGAGCGCATGGGCATGGCCTTACAGGCGCAGCTCGATCTTCGGGAAAAGCGCAAACCGGTCTAC
AACACTCTCGACCGGGCCATCGAGGCGCGGATGAAAGGGCTGGTGGCGGTCAGTCGCGAAGCCGCCGAACTGCTGGCGCA
ACGCGGACTGATGCCGGTGCCCGGCGGTTACACCTGGCGCACCGACAACCGCCTGACCCTGCCGTCGCCGCTGCGCCTGA
CCCAGGAGCAGGCGATGGCTTTCGTCCAACGCATTGCCTGCCCCGCGCAATTGGTGGTCGCCGCCGACGGCATGCTGGCC
AAACATCCGGAGCTGCTGGAACGTCTACCCTTTGACCGGGAACAGCTGCCGGGCGGGCACCATCTGCACCTGAACGATGA
GGCCGGGGCCGACCTTGTCGCAGACTGTTTCAATCGGTTCTTCGCCATTCCTTGA

Upstream 100 bases:

>100_bases
CTGGCGGCCATATGTTTCCGCTTGAGCGCCCGCAGGACACCGCTCAATTACTGAAAAACCTGTTCGATCGCTGGGAGCGC
CGGCAAGACAAGGATTGCGC

Downstream 100 bases:

>100_bases
CTTGCTCCCGGCAACTGTCGAGGCTGGGCAGGTTGAAATGGGAGACAACCAAGATGGATTTCTACACCGCTGCGACTTCA
AACTGCCCTCGAGGCACCCA

Product: epoxide hydrolase

Products: NA

Alternate protein names: Atropine acylhydrolase; Atropinesterase [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MNPAVEEVRLSLPHIELAAHLFGPEDGLPVIALHGWLDNANSFARLAPKLKGLRIVALDMAGHGHSGHRPNGAGYALWDY
AHDVLQVAEQLGWKRFGLLGHSMGAIVSLVLAGSLPERITHLALIDGVIPPTDKGENAAERMGMALQAQLDLREKRKPVY
NTLDRAIEARMKGLVAVSREAAELLAQRGLMPVPGGYTWRTDNRLTLPSPLRLTQEQAMAFVQRIACPAQLVVAADGMLA
KHPELLERLPFDREQLPGGHHLHLNDEAGADLVADCFNRFFAIP

Sequences:

>Translated_284_residues
MNPAVEEVRLSLPHIELAAHLFGPEDGLPVIALHGWLDNANSFARLAPKLKGLRIVALDMAGHGHSGHRPNGAGYALWDY
AHDVLQVAEQLGWKRFGLLGHSMGAIVSLVLAGSLPERITHLALIDGVIPPTDKGENAAERMGMALQAQLDLREKRKPVY
NTLDRAIEARMKGLVAVSREAAELLAQRGLMPVPGGYTWRTDNRLTLPSPLRLTQEQAMAFVQRIACPAQLVVAADGMLA
KHPELLERLPFDREQLPGGHHLHLNDEAGADLVADCFNRFFAIP
>Mature_284_residues
MNPAVEEVRLSLPHIELAAHLFGPEDGLPVIALHGWLDNANSFARLAPKLKGLRIVALDMAGHGHSGHRPNGAGYALWDY
AHDVLQVAEQLGWKRFGLLGHSMGAIVSLVLAGSLPERITHLALIDGVIPPTDKGENAAERMGMALQAQLDLREKRKPVY
NTLDRAIEARMKGLVAVSREAAELLAQRGLMPVPGGYTWRTDNRLTLPSPLRLTQEQAMAFVQRIACPAQLVVAADGMLA
KHPELLERLPFDREQLPGGHHLHLNDEAGADLVADCFNRFFAIP

Specific function: Alkaloid degradation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI18777795, Length=228, Percent_Identity=32.4561403508772, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI17137390, Length=293, Percent_Identity=32.0819112627986, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24667999, Length=298, Percent_Identity=27.8523489932886, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI21357291, Length=292, Percent_Identity=28.7671232876712, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24667992, Length=292, Percent_Identity=28.7671232876712, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24655874, Length=280, Percent_Identity=27.8571428571429, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21356953, Length=282, Percent_Identity=26.241134751773, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24655886, Length=126, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356937, Length=291, Percent_Identity=25.7731958762887, Blast_Score=88, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.10 [H]

Molecular weight: Translated: 31017; Mature: 31017

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPAVEEVRLSLPHIELAAHLFGPEDGLPVIALHGWLDNANSFARLAPKLKGLRIVALDM
CCCCHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEE
AGHGHSGHRPNGAGYALWDYAHDVLQVAEQLGWKRFGLLGHSMGAIVSLVLAGSLPERIT
CCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
HLALIDGVIPPTDKGENAAERMGMALQAQLDLREKRKPVYNTLDRAIEARMKGLVAVSRE
HHHHHHCCCCCCCCCCCHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AAELLAQRGLMPVPGGYTWRTDNRLTLPSPLRLTQEQAMAFVQRIACPAQLVVAADGMLA
HHHHHHHCCCCCCCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCHH
KHPELLERLPFDREQLPGGHHLHLNDEAGADLVADCFNRFFAIP
CCHHHHHHCCCCHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNPAVEEVRLSLPHIELAAHLFGPEDGLPVIALHGWLDNANSFARLAPKLKGLRIVALDM
CCCCHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEE
AGHGHSGHRPNGAGYALWDYAHDVLQVAEQLGWKRFGLLGHSMGAIVSLVLAGSLPERIT
CCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
HLALIDGVIPPTDKGENAAERMGMALQAQLDLREKRKPVYNTLDRAIEARMKGLVAVSRE
HHHHHHCCCCCCCCCCCHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AAELLAQRGLMPVPGGYTWRTDNRLTLPSPLRLTQEQAMAFVQRIACPAQLVVAADGMLA
HHHHHHHCCCCCCCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCHH
KHPELLERLPFDREQLPGGHHLHLNDEAGADLVADCFNRFFAIP
CCHHHHHHCCCCHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA