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The map label for this gene is gyrA [H]

Identifier: 77460299

GI number: 77460299

Start: 4607685

End: 4610348

Strand: Reverse

Name: gyrA [H]

Synonym: Pfl01_4078

Alternate gene names: 77460299

Gene position: 4610348-4607685 (Counterclockwise)

Preceding gene: 77460300

Following gene: 77460298

Centisome position: 71.61

GC content: 61.45

Gene sequence:

>2664_bases
ATGGGCGAACTGGCCAAAGAAATCCTCCCGGTCAATATCGAAGACGAGCTGAAACAGTCCTATCTCGACTACGCGATGAG
CGTGATCGTCGGCCGTGCACTGCCGGATGCGCGCGATGGCTTGAAGCCCGTGCACCGTCGCGTGCTGTTCGCGATGAGCG
AGCTGGGCAACGACTTTAACAAGCCGTACAAGAAATCTGCCCGTGTCGTCGGTGACGTGATCGGTAAGTATCACCCGCAC
GGTGATACCGCGGTGTACGACACCATCGTCCGCATGGCGCAGCCTTTCTCCCTGCGTTACCTGCTGGTCGACGGCCAGGG
CAACTTCGGTTCGGTGGACGGCGACAATGCCGCGGCCATGCGATACACCGAAGTGCGCATGACCAAGCTGGCGCACGAGC
TGCTGGCCGACCTGCACAAGGAAACCGTGGACTGGGTGCCGAACTACGACGGCACCGAACTGATCCCGGCGGTCATGCCG
ACCCGCGTACCGAACCTGCTGGTCAACGGTTCCAGCGGTATCGCCGTGGGCATGGCGACCAACATTCCGCCGCACAACCT
TGGTGAAGTCATCGACGGTTGCCTGGCCCTCATCGACAACCCCGAGCTGACCGTCGATGAGCTGATGCAATACATTCCCG
GTCCGGACTTCCCGACTGCCGCGATCATCAACGGTCGCGCTGGCATCATCGAAGCCTACCGCACCGGCCGTGGCCGCATT
TACATGCGCGCCCGCTCGATCATCGAAGACATCGACAAGGTCGGTGGCCGTCAGCAGATCGTCATCACCGAACTCCCTTA
CCAGCTGAACAAGGCGCGTCTGATCGAGAAGATCGCCGAGCTGGTAAAAGAGAAGAAACTCGAAGGCATCACCGAACTGC
GCGACGAGTCCGACAAGGACGGTATGCGCGTCGTGATCGAACTGCGTCGCGGCGAAGTGCCTGAGGTGATCCTCAACAAC
CTCTACGCCCAGACCCAGCTGCAATCGGTATTCGGCATCAACATCGTTGCGCTGATCGACGGCCGTCCGCGGATCCTGAA
CCTCAAGGACCTGCTGGAAGCCTTCGTCCGTCACCGTCGCGAAGTGGTTACCCGCCGTACCGTGTTCGAACTGCGCAAGG
CGCGCGAGCGTGGTCACATCCTCGAAGGTCAGGCCGTTGCCCTGTCGAACATCGACCCGGTCATCGCTCTGATCAAGGCT
TCGCCGACGCCGTCGGAAGCCAAGGAAGCGCTGGTCAGCACGCCGTGGGAATCCACTGCCGTGGTCGCGATGGTGGAGCG
TGCCGGTGCCGATTCTTGCCGTCCCGAAAACCTCGATCCGCAATACGGTCTGCGCGACGGCAAGTACTTCCTGTCGCCAG
AGCAGGCGCAAGCCATTCTGGAACTGCGTCTGCACCGCCTGACCGGTCTGGAACACGAAAAGCTGCTGGCCGAGTATCAA
GAGATCCTCAATCAGATCGGCGAGCTGATCCGCATCCTCAACAGCGCCGTGCGCCTGATGGAAGTGATCCGCGAAGAGCT
GGAAGTGATCCGCGCCGAATACGGCGACGTGCGCCGCACCGAGATCCTCGATGCCCGTCTCGACCTGACCCTGGGCGACA
TGATCCCGGAAGAAGAGCGCGTCGTGACCATTTCCCACGGCGGTTATGCCAAGACCCAGCCGCTGGCGGCTTACCAGGCC
CAGCGTCGTGGCGGTAAAGGCAAATCGGCTACCGGCGTGAAGGATGAGGACTACATCGCTCACCTGCTGGTCGCCAACAG
CCATACCACGCTGCTGCTGTTCTCCAGCAAGGGCAAGGTGTACTGGCTGAAAACCTACGAAATTCCGGAGGCGTCCCGCG
CCGCCCGTGGTCGTCCGCTGGTCAACCTGCTGCCGCTGGATGACGGTGAATACATCACCACCATGCTGCCGGTCGAGGAA
TACACCGAAGGTCACTACATCTTCATGGCCACCGCCAACGGCACCGTGAAGAAGACCCCGCTGGAATCCTTCAGCCGTCA
GCGCAGCGTCGGTCTGATCGCGCTGGAACTGGACGAAGGCGACGTGCTGATTTCTGCCGCCATCACCGATGGCGAGCGTG
AAGTGATGCTGTTCTCCGACGGCGGCAAAGTGACCCGCTTCAAGGAATCCGACGTGCGTGCCATGGGCCGTACCGCCCGC
GGTGTGCGCGGCATGCGTCTGCCGGAAGGGCAGAAGTTGATTTCGATGCTGATCCCGGAAGAAGGCAGCCAGATCCTCAC
CGCTTCCGAGCGTGGTTATGGCAAGCGTACCGCGATCACCGAGTTCCCTGAGTACAAGCGTGGCGGCCAGGGCGTGATCG
CCATGGTCAGCAACGAGCGTAACGGCCGTCTGGTCGGTGCGGTCCAGGTGCTCGACGGCGAGGAGATCATGCTGATTTCC
GACCAGGGCACGCTGGTGCGTACCCGTGTCGACGAAGTCTCCAGTCTGGGTCGTAATACCCAGGGCGTGACCCTGATCAA
ACTGGCCAAGGATGAAACCCTGGTCGGTCTGGAGCGGGTTCAGGAGCCGTCGGAAGTCGAGGGTGAAGAGCTTGAAGGTG
AAGAAGGTGTCGAACTGGAAGATGGCGTGATCGGCGCCGAGCCGGACGATGCGGTCGACAACCTGCAGGCGGACGCCGCA
GACGAAGAAGAGTCGCAAGACTAA

Upstream 100 bases:

>100_bases
TGTCGTAAGTCGGCGCATGGCACTCGGCCTGCATCGACGAGCTTCGTGCGTCCCCTATGGATATGGCGAAGTTTCACCAG
AAAAAGGAATCAGGCTTCTC

Downstream 100 bases:

>100_bases
CCAAATCGTAGCAGGGCAGAGTGTTTGCTCTGTCCGCTACCGTGATTGCATAGCGAGAGTGGATGTGAGCAAACGAGCCT
TTAACTTCTGCGCAGGTCCC

Product: DNA gyrase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 887; Mature: 886

Protein sequence:

>887_residues
MGELAKEILPVNIEDELKQSYLDYAMSVIVGRALPDARDGLKPVHRRVLFAMSELGNDFNKPYKKSARVVGDVIGKYHPH
GDTAVYDTIVRMAQPFSLRYLLVDGQGNFGSVDGDNAAAMRYTEVRMTKLAHELLADLHKETVDWVPNYDGTELIPAVMP
TRVPNLLVNGSSGIAVGMATNIPPHNLGEVIDGCLALIDNPELTVDELMQYIPGPDFPTAAIINGRAGIIEAYRTGRGRI
YMRARSIIEDIDKVGGRQQIVITELPYQLNKARLIEKIAELVKEKKLEGITELRDESDKDGMRVVIELRRGEVPEVILNN
LYAQTQLQSVFGINIVALIDGRPRILNLKDLLEAFVRHRREVVTRRTVFELRKARERGHILEGQAVALSNIDPVIALIKA
SPTPSEAKEALVSTPWESTAVVAMVERAGADSCRPENLDPQYGLRDGKYFLSPEQAQAILELRLHRLTGLEHEKLLAEYQ
EILNQIGELIRILNSAVRLMEVIREELEVIRAEYGDVRRTEILDARLDLTLGDMIPEEERVVTISHGGYAKTQPLAAYQA
QRRGGKGKSATGVKDEDYIAHLLVANSHTTLLLFSSKGKVYWLKTYEIPEASRAARGRPLVNLLPLDDGEYITTMLPVEE
YTEGHYIFMATANGTVKKTPLESFSRQRSVGLIALELDEGDVLISAAITDGEREVMLFSDGGKVTRFKESDVRAMGRTAR
GVRGMRLPEGQKLISMLIPEEGSQILTASERGYGKRTAITEFPEYKRGGQGVIAMVSNERNGRLVGAVQVLDGEEIMLIS
DQGTLVRTRVDEVSSLGRNTQGVTLIKLAKDETLVGLERVQEPSEVEGEELEGEEGVELEDGVIGAEPDDAVDNLQADAA
DEEESQD

Sequences:

>Translated_887_residues
MGELAKEILPVNIEDELKQSYLDYAMSVIVGRALPDARDGLKPVHRRVLFAMSELGNDFNKPYKKSARVVGDVIGKYHPH
GDTAVYDTIVRMAQPFSLRYLLVDGQGNFGSVDGDNAAAMRYTEVRMTKLAHELLADLHKETVDWVPNYDGTELIPAVMP
TRVPNLLVNGSSGIAVGMATNIPPHNLGEVIDGCLALIDNPELTVDELMQYIPGPDFPTAAIINGRAGIIEAYRTGRGRI
YMRARSIIEDIDKVGGRQQIVITELPYQLNKARLIEKIAELVKEKKLEGITELRDESDKDGMRVVIELRRGEVPEVILNN
LYAQTQLQSVFGINIVALIDGRPRILNLKDLLEAFVRHRREVVTRRTVFELRKARERGHILEGQAVALSNIDPVIALIKA
SPTPSEAKEALVSTPWESTAVVAMVERAGADSCRPENLDPQYGLRDGKYFLSPEQAQAILELRLHRLTGLEHEKLLAEYQ
EILNQIGELIRILNSAVRLMEVIREELEVIRAEYGDVRRTEILDARLDLTLGDMIPEEERVVTISHGGYAKTQPLAAYQA
QRRGGKGKSATGVKDEDYIAHLLVANSHTTLLLFSSKGKVYWLKTYEIPEASRAARGRPLVNLLPLDDGEYITTMLPVEE
YTEGHYIFMATANGTVKKTPLESFSRQRSVGLIALELDEGDVLISAAITDGEREVMLFSDGGKVTRFKESDVRAMGRTAR
GVRGMRLPEGQKLISMLIPEEGSQILTASERGYGKRTAITEFPEYKRGGQGVIAMVSNERNGRLVGAVQVLDGEEIMLIS
DQGTLVRTRVDEVSSLGRNTQGVTLIKLAKDETLVGLERVQEPSEVEGEELEGEEGVELEDGVIGAEPDDAVDNLQADAA
DEEESQD
>Mature_886_residues
GELAKEILPVNIEDELKQSYLDYAMSVIVGRALPDARDGLKPVHRRVLFAMSELGNDFNKPYKKSARVVGDVIGKYHPHG
DTAVYDTIVRMAQPFSLRYLLVDGQGNFGSVDGDNAAAMRYTEVRMTKLAHELLADLHKETVDWVPNYDGTELIPAVMPT
RVPNLLVNGSSGIAVGMATNIPPHNLGEVIDGCLALIDNPELTVDELMQYIPGPDFPTAAIINGRAGIIEAYRTGRGRIY
MRARSIIEDIDKVGGRQQIVITELPYQLNKARLIEKIAELVKEKKLEGITELRDESDKDGMRVVIELRRGEVPEVILNNL
YAQTQLQSVFGINIVALIDGRPRILNLKDLLEAFVRHRREVVTRRTVFELRKARERGHILEGQAVALSNIDPVIALIKAS
PTPSEAKEALVSTPWESTAVVAMVERAGADSCRPENLDPQYGLRDGKYFLSPEQAQAILELRLHRLTGLEHEKLLAEYQE
ILNQIGELIRILNSAVRLMEVIREELEVIRAEYGDVRRTEILDARLDLTLGDMIPEEERVVTISHGGYAKTQPLAAYQAQ
RRGGKGKSATGVKDEDYIAHLLVANSHTTLLLFSSKGKVYWLKTYEIPEASRAARGRPLVNLLPLDDGEYITTMLPVEEY
TEGHYIFMATANGTVKKTPLESFSRQRSVGLIALELDEGDVLISAAITDGEREVMLFSDGGKVTRFKESDVRAMGRTARG
VRGMRLPEGQKLISMLIPEEGSQILTASERGYGKRTAITEFPEYKRGGQGVIAMVSNERNGRLVGAVQVLDGEEIMLISD
QGTLVRTRVDEVSSLGRNTQGVTLIKLAKDETLVGLERVQEPSEVEGEELEGEEGVELEDGVIGAEPDDAVDNLQADAAD
EEESQD

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=321, Percent_Identity=25.5451713395639, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI19913408, Length=343, Percent_Identity=23.9067055393586, Blast_Score=72, Evalue=3e-12,
Organism=Escherichia coli, GI1788562, Length=886, Percent_Identity=66.2528216704289, Blast_Score=1195, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=729, Percent_Identity=36.3511659807956, Blast_Score=412, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17535065, Length=384, Percent_Identity=26.3020833333333, Blast_Score=73, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=349, Percent_Identity=27.5071633237822, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17136538, Length=352, Percent_Identity=26.7045454545455, Blast_Score=79, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 98192; Mature: 98061

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGELAKEILPVNIEDELKQSYLDYAMSVIVGRALPDARDGLKPVHRRVLFAMSELGNDFN
CCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHCCCCC
KPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFSLRYLLVDGQGNFGSVDGDNAAAM
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCEE
RYTEVRMTKLAHELLADLHKETVDWVPNYDGTELIPAVMPTRVPNLLVNGSSGIAVGMAT
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEECCCCEEEEEEC
NIPPHNLGEVIDGCLALIDNPELTVDELMQYIPGPDFPTAAIINGRAGIIEAYRTGRGRI
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCEEEECCCCHHHHHHHCCCCEE
YMRARSIIEDIDKVGGRQQIVITELPYQLNKARLIEKIAELVKEKKLEGITELRDESDKD
EEHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCC
GMRVVIELRRGEVPEVILNNLYAQTQLQSVFGINIVALIDGRPRILNLKDLLEAFVRHRR
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEECHHHHHHHHHHHHH
EVVTRRTVFELRKARERGHILEGQAVALSNIDPVIALIKASPTPSEAKEALVSTPWESTA
HHHHHHHHHHHHHHHHCCCEECCCEEEECCCCCEEEEEECCCCCHHHHHHHHCCCCCCHH
VVAMVERAGADSCRPENLDPQYGLRDGKYFLSPEQAQAILELRLHRLTGLEHEKLLAEYQ
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EILNQIGELIRILNSAVRLMEVIREELEVIRAEYGDVRRTEILDARLDLTLGDMIPEEER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEHHCCCCCCCC
VVTISHGGYAKTQPLAAYQAQRRGGKGKSATGVKDEDYIAHLLVANSHTTLLLFSSKGKV
EEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHEEEEEEECCCEEEEEEECCCCE
YWLKTYEIPEASRAARGRPLVNLLPLDDGEYITTMLPVEEYTEGHYIFMATANGTVKKTP
EEEEEECCCCHHHHHCCCCCEEEEECCCCCEEEEEECHHHCCCCCEEEEEECCCCEECCC
LESFSRQRSVGLIALELDEGDVLISAAITDGEREVMLFSDGGKVTRFKESDVRAMGRTAR
HHHHHHCCCCEEEEEEECCCCEEEEEEECCCCEEEEEEECCCCEEEECHHHHHHHHHHHC
GVRGMRLPEGQKLISMLIPEEGSQILTASERGYGKRTAITEFPEYKRGGQGVIAMVSNER
CCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCC
NGRLVGAVQVLDGEEIMLISDQGTLVRTRVDEVSSLGRNTQGVTLIKLAKDETLVGLERV
CCEEEEEEEEECCCEEEEECCCCCEEEHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHC
QEPSEVEGEELEGEEGVELEDGVIGAEPDDAVDNLQADAADEEESQD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
GELAKEILPVNIEDELKQSYLDYAMSVIVGRALPDARDGLKPVHRRVLFAMSELGNDFN
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHCCCCC
KPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFSLRYLLVDGQGNFGSVDGDNAAAM
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCEE
RYTEVRMTKLAHELLADLHKETVDWVPNYDGTELIPAVMPTRVPNLLVNGSSGIAVGMAT
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEECCCCEEEEEEC
NIPPHNLGEVIDGCLALIDNPELTVDELMQYIPGPDFPTAAIINGRAGIIEAYRTGRGRI
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCEEEECCCCHHHHHHHCCCCEE
YMRARSIIEDIDKVGGRQQIVITELPYQLNKARLIEKIAELVKEKKLEGITELRDESDKD
EEHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCC
GMRVVIELRRGEVPEVILNNLYAQTQLQSVFGINIVALIDGRPRILNLKDLLEAFVRHRR
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEECHHHHHHHHHHHHH
EVVTRRTVFELRKARERGHILEGQAVALSNIDPVIALIKASPTPSEAKEALVSTPWESTA
HHHHHHHHHHHHHHHHCCCEECCCEEEECCCCCEEEEEECCCCCHHHHHHHHCCCCCCHH
VVAMVERAGADSCRPENLDPQYGLRDGKYFLSPEQAQAILELRLHRLTGLEHEKLLAEYQ
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EILNQIGELIRILNSAVRLMEVIREELEVIRAEYGDVRRTEILDARLDLTLGDMIPEEER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEHHCCCCCCCC
VVTISHGGYAKTQPLAAYQAQRRGGKGKSATGVKDEDYIAHLLVANSHTTLLLFSSKGKV
EEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHEEEEEEECCCEEEEEEECCCCE
YWLKTYEIPEASRAARGRPLVNLLPLDDGEYITTMLPVEEYTEGHYIFMATANGTVKKTP
EEEEEECCCCHHHHHCCCCCEEEEECCCCCEEEEEECHHHCCCCCEEEEEECCCCEECCC
LESFSRQRSVGLIALELDEGDVLISAAITDGEREVMLFSDGGKVTRFKESDVRAMGRTAR
HHHHHHCCCCEEEEEEECCCCEEEEEEECCCCEEEEEEECCCCEEEECHHHHHHHHHHHC
GVRGMRLPEGQKLISMLIPEEGSQILTASERGYGKRTAITEFPEYKRGGQGVIAMVSNER
CCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCC
NGRLVGAVQVLDGEEIMLISDQGTLVRTRVDEVSSLGRNTQGVTLIKLAKDETLVGLERV
CCEEEEEEEEECCCEEEEECCCCCEEEHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHC
QEPSEVEGEELEGEEGVELEDGVIGAEPDDAVDNLQADAADEEESQD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7811002; 10984043 [H]