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The map label for this gene is pheA [H]

Identifier: 77460297

GI number: 77460297

Start: 4605441

End: 4606535

Strand: Reverse

Name: pheA [H]

Synonym: Pfl01_4076

Alternate gene names: 77460297

Gene position: 4606535-4605441 (Counterclockwise)

Preceding gene: 77460298

Following gene: 77460296

Centisome position: 71.55

GC content: 61.1

Gene sequence:

>1095_bases
ATGTCCGAGCAGGAGCTCAAGGCGCTGCGCGTTCGCATCGATAACCTGGATGAGAAGATCCTCCAGCTGATCAGCGATCG
CGCGCGCTGCGCCGAAGAAGTTGCCCGGGTCAAGATGGCCAGTCTGGCCGAAGGCGAGCAGCCCGTGTTCTATCGTCCCG
AGCGTGAGGCGCAGGTCCTTAAGCGCGTGATGGAGCGCAACAAGGGACCACTGGGCAACGAAGAGATGGCGCGCTTGTTC
CGCGAAATCATGTCTTCGTGCCTGGCCCTCGAGAACCCGCTGAAAGTGGCTTATCTCGGTCCGGAAGGCACCTTCACCCA
GGCGGCGGCCATGAAGCACTTCGGTCACGCGGTGATCAGCAAGCCGATGGCGGCGATCGACGAAGTGTTCCGTGAAGTGG
CGGCCGGTGCGGTGAACTTTGGCGTGGTGCCGGTGGAGAACTCCACCGAAGGCGCGGTCAACCACACCCTCGACAGCTTC
CTCGAGCACGACATGGTGATCTGCGGCGAAGTCGAGCTGCGCATTCACCACCACCTGCTGGTCGGTGAAAACACCAAGAC
CGACAGCATCAGCCGTATCTATTCCCACGCCCAGTCGCTGGCCCAGTGCCGCAAATGGCTGGACGCCCATTATCCGAATG
TCGAGCGCGTGGCGGTTTCCAGCAACGCCGAGGCGGCCAAGCGGGTCAAGGGCGAATGGAACTCGGCGGCGATCGCCGGT
GACATGGCGGCCGGGCTCTATGGCCTGACCCGTCTGGCCGAGAAGATCGAGGATCGCCCGGACAACTCCACGCGCTTCCT
GATGATCGGTAATCAGGAAGTGCCGCCGACCGGCGATGACAAGACTTCGATCATCGTGTCCATGAGCAACAAGCCCGGCG
CTCTCCACGAGCTGCTGGTGCCGTTCCATGACAACGGGATCGACCTGACCCGGATCGAAACCCGTCCGTCGCGCAGCGGC
AAATGGACCTACGTGTTTTTCATCGACTTCGTGGGCCATCACCGTGATCCGCTGATCAAGGGTGTACTGGAAAAGATCAG
TCAGGAAGCAGTAGCACTCAAGGTGCTGGGTTCCTACCCGAAAGCAGTTCTTTAA

Upstream 100 bases:

>100_bases
CGGTCGGCGGCATGCGTGCCTCGATCTACAACGCCCTGGGTCTGGACGCCGTCGAAGCGCTGGTCGGCTACATGGCTGAA
TTCGAGAAGGAGCATTCCTG

Downstream 100 bases:

>100_bases
GGGGTGGTAGCAATGAGTGGCAACTTCCTCGCTCTGGCACAGCCGGGCGTGCAGCAACTTTCGCCATACGTGCCGGGCAA
GCCCGTGGACGAACTGGCCC

Product: prephenate dehydratase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSEQELKALRVRIDNLDEKILQLISDRARCAEEVARVKMASLAEGEQPVFYRPEREAQVLKRVMERNKGPLGNEEMARLF
REIMSSCLALENPLKVAYLGPEGTFTQAAAMKHFGHAVISKPMAAIDEVFREVAAGAVNFGVVPVENSTEGAVNHTLDSF
LEHDMVICGEVELRIHHHLLVGENTKTDSISRIYSHAQSLAQCRKWLDAHYPNVERVAVSSNAEAAKRVKGEWNSAAIAG
DMAAGLYGLTRLAEKIEDRPDNSTRFLMIGNQEVPPTGDDKTSIIVSMSNKPGALHELLVPFHDNGIDLTRIETRPSRSG
KWTYVFFIDFVGHHRDPLIKGVLEKISQEAVALKVLGSYPKAVL

Sequences:

>Translated_364_residues
MSEQELKALRVRIDNLDEKILQLISDRARCAEEVARVKMASLAEGEQPVFYRPEREAQVLKRVMERNKGPLGNEEMARLF
REIMSSCLALENPLKVAYLGPEGTFTQAAAMKHFGHAVISKPMAAIDEVFREVAAGAVNFGVVPVENSTEGAVNHTLDSF
LEHDMVICGEVELRIHHHLLVGENTKTDSISRIYSHAQSLAQCRKWLDAHYPNVERVAVSSNAEAAKRVKGEWNSAAIAG
DMAAGLYGLTRLAEKIEDRPDNSTRFLMIGNQEVPPTGDDKTSIIVSMSNKPGALHELLVPFHDNGIDLTRIETRPSRSG
KWTYVFFIDFVGHHRDPLIKGVLEKISQEAVALKVLGSYPKAVL
>Mature_363_residues
SEQELKALRVRIDNLDEKILQLISDRARCAEEVARVKMASLAEGEQPVFYRPEREAQVLKRVMERNKGPLGNEEMARLFR
EIMSSCLALENPLKVAYLGPEGTFTQAAAMKHFGHAVISKPMAAIDEVFREVAAGAVNFGVVPVENSTEGAVNHTLDSFL
EHDMVICGEVELRIHHHLLVGENTKTDSISRIYSHAQSLAQCRKWLDAHYPNVERVAVSSNAEAAKRVKGEWNSAAIAGD
MAAGLYGLTRLAEKIEDRPDNSTRFLMIGNQEVPPTGDDKTSIIVSMSNKPGALHELLVPFHDNGIDLTRIETRPSRSGK
WTYVFFIDFVGHHRDPLIKGVLEKISQEAVALKVLGSYPKAVL

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=386, Percent_Identity=29.7927461139896, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010957
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01842 ACT; PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 40402; Mature: 40271

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00857 PREPHENATE_DEHYDR_1 ; PS00858 PREPHENATE_DEHYDR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQELKALRVRIDNLDEKILQLISDRARCAEEVARVKMASLAEGEQPVFYRPEREAQVL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHH
KRVMERNKGPLGNEEMARLFREIMSSCLALENPLKVAYLGPEGTFTQAAAMKHFGHAVIS
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
KPMAAIDEVFREVAAGAVNFGVVPVENSTEGAVNHTLDSFLEHDMVICGEVELRIHHHLL
HHHHHHHHHHHHHHCCCHHCCEEECCCCCCCHHHHHHHHHHHCCEEEEEEEEEEEEEEEE
VGENTKTDSISRIYSHAQSLAQCRKWLDAHYPNVERVAVSSNAEAAKRVKGEWNSAAIAG
ECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCCCCHHHHH
DMAAGLYGLTRLAEKIEDRPDNSTRFLMIGNQEVPPTGDDKTSIIVSMSNKPGALHELLV
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHC
PFHDNGIDLTRIETRPSRSGKWTYVFFIDFVGHHRDPLIKGVLEKISQEAVALKVLGSYP
CCCCCCEEEEEEECCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
KAVL
CCCC
>Mature Secondary Structure 
SEQELKALRVRIDNLDEKILQLISDRARCAEEVARVKMASLAEGEQPVFYRPEREAQVL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHH
KRVMERNKGPLGNEEMARLFREIMSSCLALENPLKVAYLGPEGTFTQAAAMKHFGHAVIS
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
KPMAAIDEVFREVAAGAVNFGVVPVENSTEGAVNHTLDSFLEHDMVICGEVELRIHHHLL
HHHHHHHHHHHHHHCCCHHCCEEECCCCCCCHHHHHHHHHHHCCEEEEEEEEEEEEEEEE
VGENTKTDSISRIYSHAQSLAQCRKWLDAHYPNVERVAVSSNAEAAKRVKGEWNSAAIAG
ECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCCCCHHHHH
DMAAGLYGLTRLAEKIEDRPDNSTRFLMIGNQEVPPTGDDKTSIIVSMSNKPGALHELLV
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHC
PFHDNGIDLTRIETRPSRSGKWTYVFFIDFVGHHRDPLIKGVLEKISQEAVALKVLGSYP
CCCCCCEEEEEEECCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
KAVL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]