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The map label for this gene is hlyB [H]

Identifier: 77460243

GI number: 77460243

Start: 4546814

End: 4548295

Strand: Direct

Name: hlyB [H]

Synonym: Pfl01_4022

Alternate gene names: 77460243

Gene position: 4546814-4548295 (Clockwise)

Preceding gene: 77460242

Following gene: 77460244

Centisome position: 70.62

GC content: 63.56

Gene sequence:

>1482_bases
ATGGGTGCCTGGCTTAGCAATATCTCGCTGAAATACAAATTCTGGGCGGTCAACGCGGTCGCCTTTGTCACCACCCTGTT
GCTGGTGCTGTACGCCGTGCAGCTCGAACAACAGGCCCGCAGCCACGCCGCGCAAACCTCGGCCCAGGCCCAGGCGCAAT
TGCTCAAGGCCTGGCCGGTCGGGCAGGCGCTGCCCAAATCCGATCAGGTACTGACGTTTAAACGCGGTGAAGCGCCGCGC
CTCAACGATCAACCGCTGCTGGAGATAACCGACAGCAGCGGCTGGATCGAGATCAGTCATTTGCCGTTGTTCGGCGAAAA
TCCGCTGCTCGGCGCCGAAGTCTTCAGCCGTGCCGATGGCCAGCAGGTCGCCGTGATCGCCTACGCCCCCAGCCTCAGCC
AGGTGTTCAGCGAGCGCTTCGCCAACTACGCGGTGGCGGTGTTCATCCTGATGCTGGCGATGCTCGGTGCCTCGCAGCTA
CTGATCCGCTTTCTGCTCAGCCAGCTCAATACGCTCAAGGACGTGATGCTGCACGTGGAGAAAACCGGCGACCTCGCCGC
CCGGGTGCCGCTGGCCTGCAAGGACGAAGTCGGGCAAATGGCCAACGCGTTCAATGCGATGCAGGCCGGTTATCAGCGAG
TGGTGACCACCGTGGCCAACACCGCGCGGCAACTCGACGTCGGCGCTGCGCGACTGGCGTCGAGCATGAACGAGGTGCGT
CACGGCATGCTTGGCCAGCAGAGTGAAACCGATCAGGCCGCCACGGCGATCAACGAAATGACCGCGACTGTCTACCACAT
CGCCCAGCACGCCGGCGCCACTCGCGATCTGTCGCAGACCGCCGACGGCCTGGCCGGCAGCGGCCAGCAAGTGGTGAGCC
GGGTGCAGACGTCGATTGCCGGCCTGTCCAGCGGCGTGCAGCAGACTGCCGAGATGATCCAGCGTCTGGCCGAGGACAGC
CAGAAGATCAACGGCGTGGTCAGCGTGATCCACAGCATCGCCGAACAGACCAATTTGCTGGCACTCAATGCCGCCATCGA
AGCGGCCCGTGCCGGGGAAATGGGTCGCGGGTTTGCGGTGGTCGCCGACGAGGTGCGCAACCTCGCAAAACGCGTGCAGA
CCTCCACCGATGAAATCACCACCATGGTCTCGGCGTTGCAGGCCGGCACCCGCGACGCGGTGGACTTCATGCAGGAGAGT
TCGTACAAGGCCGATGACTGCGTGCAGCAGGCGCAAGAAGCCGGCGAAGCGCTGGCGGAAATCACCGGGGCGGTAGCGCA
GATGCGCGAGAGCAACACGCAGATTGCGGTGGCGGCGGAGCAGCAAAGTCAGGTGGCCGAAGAGATGAACCGCGCGGTGG
TGAGCATTCGCGACGTGACCGAAAACACTGTGCAACAGACCGTGGATTCGGCGACCACGAGCAATGAACTGGCGACGCTA
GCCGGGGAGCTGAACAAGGCGATCGGTCAGTTGAAGCTCTAG

Upstream 100 bases:

>100_bases
GATGCATCGGTTGTTGATGCATATCAAGAACCGGACGCCTGGGTAGCGGCACAATAATGGCACCTTGCCAAAACTGTTTC
CGCTATCAGAGAAGACCTCC

Downstream 100 bases:

>100_bases
GGGGCAACATCGAGATTGGCCATTGTCCCTATCACTTCAATAGCCAACCTTGATTCGCCGCCCTCGCTGCCCGGGCCTAT
TCTTCAACCATGTACTGAAC

Product: chemotaxis sensory transducer

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MGAWLSNISLKYKFWAVNAVAFVTTLLLVLYAVQLEQQARSHAAQTSAQAQAQLLKAWPVGQALPKSDQVLTFKRGEAPR
LNDQPLLEITDSSGWIEISHLPLFGENPLLGAEVFSRADGQQVAVIAYAPSLSQVFSERFANYAVAVFILMLAMLGASQL
LIRFLLSQLNTLKDVMLHVEKTGDLAARVPLACKDEVGQMANAFNAMQAGYQRVVTTVANTARQLDVGAARLASSMNEVR
HGMLGQQSETDQAATAINEMTATVYHIAQHAGATRDLSQTADGLAGSGQQVVSRVQTSIAGLSSGVQQTAEMIQRLAEDS
QKINGVVSVIHSIAEQTNLLALNAAIEAARAGEMGRGFAVVADEVRNLAKRVQTSTDEITTMVSALQAGTRDAVDFMQES
SYKADDCVQQAQEAGEALAEITGAVAQMRESNTQIAVAAEQQSQVAEEMNRAVVSIRDVTENTVQQTVDSATTSNELATL
AGELNKAIGQLKL

Sequences:

>Translated_493_residues
MGAWLSNISLKYKFWAVNAVAFVTTLLLVLYAVQLEQQARSHAAQTSAQAQAQLLKAWPVGQALPKSDQVLTFKRGEAPR
LNDQPLLEITDSSGWIEISHLPLFGENPLLGAEVFSRADGQQVAVIAYAPSLSQVFSERFANYAVAVFILMLAMLGASQL
LIRFLLSQLNTLKDVMLHVEKTGDLAARVPLACKDEVGQMANAFNAMQAGYQRVVTTVANTARQLDVGAARLASSMNEVR
HGMLGQQSETDQAATAINEMTATVYHIAQHAGATRDLSQTADGLAGSGQQVVSRVQTSIAGLSSGVQQTAEMIQRLAEDS
QKINGVVSVIHSIAEQTNLLALNAAIEAARAGEMGRGFAVVADEVRNLAKRVQTSTDEITTMVSALQAGTRDAVDFMQES
SYKADDCVQQAQEAGEALAEITGAVAQMRESNTQIAVAAEQQSQVAEEMNRAVVSIRDVTENTVQQTVDSATTSNELATL
AGELNKAIGQLKL
>Mature_492_residues
GAWLSNISLKYKFWAVNAVAFVTTLLLVLYAVQLEQQARSHAAQTSAQAQAQLLKAWPVGQALPKSDQVLTFKRGEAPRL
NDQPLLEITDSSGWIEISHLPLFGENPLLGAEVFSRADGQQVAVIAYAPSLSQVFSERFANYAVAVFILMLAMLGASQLL
IRFLLSQLNTLKDVMLHVEKTGDLAARVPLACKDEVGQMANAFNAMQAGYQRVVTTVANTARQLDVGAARLASSMNEVRH
GMLGQQSETDQAATAINEMTATVYHIAQHAGATRDLSQTADGLAGSGQQVVSRVQTSIAGLSSGVQQTAEMIQRLAEDSQ
KINGVVSVIHSIAEQTNLLALNAAIEAARAGEMGRGFAVVADEVRNLAKRVQTSTDEITTMVSALQAGTRDAVDFMQESS
YKADDCVQQAQEAGEALAEITGAVAQMRESNTQIAVAAEQQSQVAEEMNRAVVSIRDVTENTVQQTVDSATTSNELATLA
GELNKAIGQLKL

Specific function: May form a pore through which the hemolysin can be exported [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=420, Percent_Identity=31.1904761904762, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1787690, Length=353, Percent_Identity=29.1784702549575, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788195, Length=302, Percent_Identity=32.1192052980132, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1788194, Length=413, Percent_Identity=27.3607748184019, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789453, Length=307, Percent_Identity=28.0130293159609, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660
- InterPro:   IPR000727 [H]

Pfam domain/function: PF00672 HAMP; PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 52716; Mature: 52585

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAWLSNISLKYKFWAVNAVAFVTTLLLVLYAVQLEQQARSHAAQTSAQAQAQLLKAWPV
CCCCHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GQALPKSDQVLTFKRGEAPRLNDQPLLEITDSSGWIEISHLPLFGENPLLGAEVFSRADG
CCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCHHHHHHHCCCC
QQVAVIAYAPSLSQVFSERFANYAVAVFILMLAMLGASQLLIRFLLSQLNTLKDVMLHVE
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
KTGDLAARVPLACKDEVGQMANAFNAMQAGYQRVVTTVANTARQLDVGAARLASSMNEVR
CCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGMLGQQSETDQAATAINEMTATVYHIAQHAGATRDLSQTADGLAGSGQQVVSRVQTSIA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHH
GLSSGVQQTAEMIQRLAEDSQKINGVVSVIHSIAEQTNLLALNAAIEAARAGEMGRGFAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHH
VADEVRNLAKRVQTSTDEITTMVSALQAGTRDAVDFMQESSYKADDCVQQAQEAGEALAE
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ITGAVAQMRESNTQIAVAAEQQSQVAEEMNRAVVSIRDVTENTVQQTVDSATTSNELATL
HHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AGELNKAIGQLKL
HHHHHHHHHCCCC
>Mature Secondary Structure 
GAWLSNISLKYKFWAVNAVAFVTTLLLVLYAVQLEQQARSHAAQTSAQAQAQLLKAWPV
CCCHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GQALPKSDQVLTFKRGEAPRLNDQPLLEITDSSGWIEISHLPLFGENPLLGAEVFSRADG
CCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCHHHHHHHCCCC
QQVAVIAYAPSLSQVFSERFANYAVAVFILMLAMLGASQLLIRFLLSQLNTLKDVMLHVE
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
KTGDLAARVPLACKDEVGQMANAFNAMQAGYQRVVTTVANTARQLDVGAARLASSMNEVR
CCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGMLGQQSETDQAATAINEMTATVYHIAQHAGATRDLSQTADGLAGSGQQVVSRVQTSIA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHH
GLSSGVQQTAEMIQRLAEDSQKINGVVSVIHSIAEQTNLLALNAAIEAARAGEMGRGFAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHH
VADEVRNLAKRVQTSTDEITTMVSALQAGTRDAVDFMQESSYKADDCVQQAQEAGEALAE
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ITGAVAQMRESNTQIAVAAEQQSQVAEEMNRAVVSIRDVTENTVQQTVDSATTSNELATL
HHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AGELNKAIGQLKL
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2162464; 10952301 [H]