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The map label for this gene is amtB [C]

Identifier: 77460189

GI number: 77460189

Start: 4493353

End: 4494561

Strand: Reverse

Name: amtB [C]

Synonym: Pfl01_3968

Alternate gene names: 77460189

Gene position: 4494561-4493353 (Counterclockwise)

Preceding gene: 77460198

Following gene: 77460187

Centisome position: 69.81

GC content: 62.86

Gene sequence:

>1209_bases
ATGGAAAATCTGCAAAGCGCTGTGGACACGCTGGTTCACAGTTCCAACACGTTGTTCATTCTGATCGGTGCGGTCATGGT
GCTGGCCATGCACGCCGGGTTCGCATTTCTGGAGGTCGGAACGGTTCGCCAGAAAAACCAGGTCAACGCACTGTCGAAGA
TTCTCAGCGACTTTGCGGTGTCGACGCTGGCTTATTTCTTTATAGGCTATTGGCTTTCGTACGGGGTGACCTTCCTGCAT
CCGGCGGCGGTGCTCAGTGCCGATCATGGTTATGGACTGGTGAAGTTTTTCTTCCTGCTGACCTTTGCGGCGGCGATCCC
GGCGATCATCTCCGGCGGCATCGCCGAGCGGGCGCGTTTTGTGCCGCAGTTGTGCGCCACGGCGCTGATCGTGGCGTTCA
TCTACCCGTTTTTCGAAGGGATGATCTGGAACGGCAACTACGGTCTGCAAGCCTGGCTGACCGCACAGTTCGGCGCCCCT
TTCCATGATTTTGCGGGTTCGGTGGTGGTGCATGCCATGGGCGGCTGGCTGGCGCTGGCGGCGGTGTTGCTGCTCGGCCC
GCGCACCGGACGCTATCGCGATGGCAAGCTGGTGGCGTTCGCGCCGTCGAGCATTCCGTTTCTGGCCTTGGGGTCGTGGA
TCCTGATCGTCGGCTGGTTTGGCTTCAACGTGATGAGCGCGCAAACCTTGCAAGGGGTCAGCGGACTGGTGGCGGTCAAC
TCGCTGATGGCTATGGTCGGCGGCACGGTGGCGGCGTTGATCGTCGGGCGCAACGATCCGGGCTTCCTGCACAACGGCCC
GTTGGCCGGGCTGGTGGCGGTGTGTGCCGGTTCCGACCTGATGCACCCGGTCGGCGCGCTGGTGAACGGCGCGATTGCCG
GCGCCCTGTTTGTCTGGTGCTTTACGGCGGCGCAGGTCAAATGGCGCATCGATGACGTGCTGGGTGTCTGGCCGTTGCAC
GGTCTGTGTGGCGTGTGGGGCGGCATTGCCTGCGGGATCTTCGGCTCGACCACGTTGGGCGGACTCGGCGGCGTCAGCCT
GATCAGCCAGTTGATCGGCACCGCCCTTGGTGTGGCGGTGGCGCTGATCGGCGGTTTCGCCGTCTACGGTTCGATCAAGG
CGCTGCATGGTCTGCGCCTGAGCCAGGAACAGGAGTACTACGGCGCCGACCTGTCGCTGCACAAGATCGGCGCGGTGAGC
CAGGACTAG

Upstream 100 bases:

>100_bases
ACCGGACTACCGGATCAGTGATCGCGCAGTCGCAACTTGGTGCATGAAATGTGCGCATGAACCGCTTTGGTGCCTTTTTC
AGCCTTGGGTCTGCCTCCTC

Downstream 100 bases:

>100_bases
GTTTCCTCATCGTCGGCTCCGGGGTAAAAGCCGTGAAGCAGGCGATAACGATCGATGCGCACCTGATCGACTTTCTCCTG
CACCCGGTGCGCGGGAAACC

Product: Rh-like protein/ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MENLQSAVDTLVHSSNTLFILIGAVMVLAMHAGFAFLEVGTVRQKNQVNALSKILSDFAVSTLAYFFIGYWLSYGVTFLH
PAAVLSADHGYGLVKFFFLLTFAAAIPAIISGGIAERARFVPQLCATALIVAFIYPFFEGMIWNGNYGLQAWLTAQFGAP
FHDFAGSVVVHAMGGWLALAAVLLLGPRTGRYRDGKLVAFAPSSIPFLALGSWILIVGWFGFNVMSAQTLQGVSGLVAVN
SLMAMVGGTVAALIVGRNDPGFLHNGPLAGLVAVCAGSDLMHPVGALVNGAIAGALFVWCFTAAQVKWRIDDVLGVWPLH
GLCGVWGGIACGIFGSTTLGGLGGVSLISQLIGTALGVAVALIGGFAVYGSIKALHGLRLSQEQEYYGADLSLHKIGAVS
QD

Sequences:

>Translated_402_residues
MENLQSAVDTLVHSSNTLFILIGAVMVLAMHAGFAFLEVGTVRQKNQVNALSKILSDFAVSTLAYFFIGYWLSYGVTFLH
PAAVLSADHGYGLVKFFFLLTFAAAIPAIISGGIAERARFVPQLCATALIVAFIYPFFEGMIWNGNYGLQAWLTAQFGAP
FHDFAGSVVVHAMGGWLALAAVLLLGPRTGRYRDGKLVAFAPSSIPFLALGSWILIVGWFGFNVMSAQTLQGVSGLVAVN
SLMAMVGGTVAALIVGRNDPGFLHNGPLAGLVAVCAGSDLMHPVGALVNGAIAGALFVWCFTAAQVKWRIDDVLGVWPLH
GLCGVWGGIACGIFGSTTLGGLGGVSLISQLIGTALGVAVALIGGFAVYGSIKALHGLRLSQEQEYYGADLSLHKIGAVS
QD
>Mature_402_residues
MENLQSAVDTLVHSSNTLFILIGAVMVLAMHAGFAFLEVGTVRQKNQVNALSKILSDFAVSTLAYFFIGYWLSYGVTFLH
PAAVLSADHGYGLVKFFFLLTFAAAIPAIISGGIAERARFVPQLCATALIVAFIYPFFEGMIWNGNYGLQAWLTAQFGAP
FHDFAGSVVVHAMGGWLALAAVLLLGPRTGRYRDGKLVAFAPSSIPFLALGSWILIVGWFGFNVMSAQTLQGVSGLVAVN
SLMAMVGGTVAALIVGRNDPGFLHNGPLAGLVAVCAGSDLMHPVGALVNGAIAGALFVWCFTAAQVKWRIDDVLGVWPLH
GLCGVWGGIACGIFGSTTLGGLGGVSLISQLIGTALGVAVALIGGFAVYGSIKALHGLRLSQEQEYYGADLSLHKIGAVS
QD

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=425, Percent_Identity=30.1176470588235, Blast_Score=139, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17550180, Length=417, Percent_Identity=29.9760191846523, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17550182, Length=427, Percent_Identity=28.5714285714286, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32564042, Length=428, Percent_Identity=28.5046728971963, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI133931100, Length=358, Percent_Identity=27.9329608938547, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6325396, Length=427, Percent_Identity=26.2295081967213, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6321559, Length=423, Percent_Identity=25.0591016548463, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324187, Length=376, Percent_Identity=27.9255319148936, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI161078314, Length=422, Percent_Identity=31.9905213270142, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355411, Length=248, Percent_Identity=29.0322580645161, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 42084; Mature: 42084

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLQSAVDTLVHSSNTLFILIGAVMVLAMHAGFAFLEVGTVRQKNQVNALSKILSDFAV
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHEEECCHHHHHHHHHHHHHHHHHHH
STLAYFFIGYWLSYGVTFLHPAAVLSADHGYGLVKFFFLLTFAAAIPAIISGGIAERARF
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VPQLCATALIVAFIYPFFEGMIWNGNYGLQAWLTAQFGAPFHDFAGSVVVHAMGGWLALA
HHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
AVLLLGPRTGRYRDGKLVAFAPSSIPFLALGSWILIVGWFGFNVMSAQTLQGVSGLVAVN
HHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLMAMVGGTVAALIVGRNDPGFLHNGPLAGLVAVCAGSDLMHPVGALVNGAIAGALFVWC
HHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
FTAAQVKWRIDDVLGVWPLHGLCGVWGGIACGIFGSTTLGGLGGVSLISQLIGTALGVAV
HHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
ALIGGFAVYGSIKALHGLRLSQEQEYYGADLSLHKIGAVSQD
HHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHCCCCCCC
>Mature Secondary Structure
MENLQSAVDTLVHSSNTLFILIGAVMVLAMHAGFAFLEVGTVRQKNQVNALSKILSDFAV
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHEEECCHHHHHHHHHHHHHHHHHHH
STLAYFFIGYWLSYGVTFLHPAAVLSADHGYGLVKFFFLLTFAAAIPAIISGGIAERARF
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VPQLCATALIVAFIYPFFEGMIWNGNYGLQAWLTAQFGAPFHDFAGSVVVHAMGGWLALA
HHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
AVLLLGPRTGRYRDGKLVAFAPSSIPFLALGSWILIVGWFGFNVMSAQTLQGVSGLVAVN
HHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLMAMVGGTVAALIVGRNDPGFLHNGPLAGLVAVCAGSDLMHPVGALVNGAIAGALFVWC
HHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
FTAAQVKWRIDDVLGVWPLHGLCGVWGGIACGIFGSTTLGGLGGVSLISQLIGTALGVAV
HHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
ALIGGFAVYGSIKALHGLRLSQEQEYYGADLSLHKIGAVSQD
HHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]