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The map label for this gene is moeA [H]

Identifier: 77460181

GI number: 77460181

Start: 4486955

End: 4488172

Strand: Reverse

Name: moeA [H]

Synonym: Pfl01_3960

Alternate gene names: 77460181

Gene position: 4488172-4486955 (Counterclockwise)

Preceding gene: 77460182

Following gene: 255961265

Centisome position: 69.71

GC content: 60.84

Gene sequence:

>1218_bases
GTGGGTAAGCCGGGCAAGACTGGCAGCCTGATGCCTGTCGAAGCAGCATTGACGCGGTTGCTGGAAATGGCCGAGGGCTC
GCGGATTGTCGAGAGTGAGTTTTTGCCTCTAGCGCAGGTTCAGGGGCGGGTGCTGGCGCAGGATCTGGTTTCGACGCTGG
ATCTGCCGCCATGGCCGAACAGCGCCATGGACGGTTATGCCTTGAATCTGGCCGACTGGACCGGCGAGCCGTTGCCGGTC
AGCCAGAAAGTCTTCGCCGGACAGGCACCGGAACCCTTGAAACCACGCACCTGTGCCCGGATCTTTACCGGCGCGCCGGT
GCCAGCCGGTGCAGACTGTGTCGAGATGCAGGAAAACGTCGAGATTCATGCCGACGAGCGCGTGAGTTTCACCGAGACGA
TGGTGTCCGGGCAGAACATTCGTCCACAAGGCCAGGAAACGACTGTCGGTGAGTTGGTCTTGCCTGCCGGAACCCGGCTG
GGTCCGATCGAGCAAGGGCTGGCTGCGTCGCTGGGATGCGCTGGGCTCGAGGTGGTTCGTCGGGTTCGTGTGGCAGTGCT
GTCCACGGGGGACGAGCTGGTTGAGCCGGGCACGCCACTCGGGCCGGGACAGATCTACAACAGCAACCGGGTGTTGTTGT
GCAGCTGGTTGCAGCGCCTCGGATGTGAAGTGGTGGATGCCGGCATTCTGCCTGATGACCTGTCCACCACTCGGGCGCGA
CTTGGGGAGTTGCAGGACGTAGACCTGATTCTGTCGACAGGTGGCGTGTCGGTCGGGGAAGCGGATTTCCTCGGAATCGC
TTTGAGGGAAGAAGGCGAGCTGACACTCTGGAAACTGGCAATCAAACCCGGCAAGCCGCTGACGGTCGGGCATTTTCGTG
GAGTTCCGGTGATTGGATTGCCGGGAAATCCTGCCTCGACACTGGTGACTTTTGCTTTGTTGACCCGACCTTATCTGTTG
CGTCTCCAAGGCGTGCAGGATGTCGAGCCGCTGAAGTTTTCAGTTCCTGCAGGATTTGTCTGGCCTCAGGCCGGTAACCG
CCGTGAATTTCTGCGTGGGCGCCTGGAGCAGGGACAAGCGATTATCTACCGTAACCAGAGTTCGGGTGTTTTGCGCAGCG
CTGCCTGGGCTGAGGGGCTGGTGGAAGTGCGGGAAGGGCGAGTATTGGTCAAGGGAGACCAGGTGCTATTCATTCCACTC
AGCGAGCTCCTCGGTTGA

Upstream 100 bases:

>100_bases
CGCACCGGTTGGGATGGCATCCTCGCCGAGCAACTGGACTCACGCCACCGCCCGTGCAATTTCGTCACTCATTTGAAACA
GGCGGCGCCTTGTGAATCCC

Downstream 100 bases:

>100_bases
TTGCAGGCATTTGAAAGGCAGGCACAGTCGCTAAACGATGCCTGCCTTTCAGCGGGTCAACGGCTGAGCAGTGTGACGAG
TTGATCGAACCGGCTGTTGG

Product: molybdopterin molybdochelatase

Products: NA

Alternate protein names: MPT Mo-transferase [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MGKPGKTGSLMPVEAALTRLLEMAEGSRIVESEFLPLAQVQGRVLAQDLVSTLDLPPWPNSAMDGYALNLADWTGEPLPV
SQKVFAGQAPEPLKPRTCARIFTGAPVPAGADCVEMQENVEIHADERVSFTETMVSGQNIRPQGQETTVGELVLPAGTRL
GPIEQGLAASLGCAGLEVVRRVRVAVLSTGDELVEPGTPLGPGQIYNSNRVLLCSWLQRLGCEVVDAGILPDDLSTTRAR
LGELQDVDLILSTGGVSVGEADFLGIALREEGELTLWKLAIKPGKPLTVGHFRGVPVIGLPGNPASTLVTFALLTRPYLL
RLQGVQDVEPLKFSVPAGFVWPQAGNRREFLRGRLEQGQAIIYRNQSSGVLRSAAWAEGLVEVREGRVLVKGDQVLFIPL
SELLG

Sequences:

>Translated_405_residues
MGKPGKTGSLMPVEAALTRLLEMAEGSRIVESEFLPLAQVQGRVLAQDLVSTLDLPPWPNSAMDGYALNLADWTGEPLPV
SQKVFAGQAPEPLKPRTCARIFTGAPVPAGADCVEMQENVEIHADERVSFTETMVSGQNIRPQGQETTVGELVLPAGTRL
GPIEQGLAASLGCAGLEVVRRVRVAVLSTGDELVEPGTPLGPGQIYNSNRVLLCSWLQRLGCEVVDAGILPDDLSTTRAR
LGELQDVDLILSTGGVSVGEADFLGIALREEGELTLWKLAIKPGKPLTVGHFRGVPVIGLPGNPASTLVTFALLTRPYLL
RLQGVQDVEPLKFSVPAGFVWPQAGNRREFLRGRLEQGQAIIYRNQSSGVLRSAAWAEGLVEVREGRVLVKGDQVLFIPL
SELLG
>Mature_404_residues
GKPGKTGSLMPVEAALTRLLEMAEGSRIVESEFLPLAQVQGRVLAQDLVSTLDLPPWPNSAMDGYALNLADWTGEPLPVS
QKVFAGQAPEPLKPRTCARIFTGAPVPAGADCVEMQENVEIHADERVSFTETMVSGQNIRPQGQETTVGELVLPAGTRLG
PIEQGLAASLGCAGLEVVRRVRVAVLSTGDELVEPGTPLGPGQIYNSNRVLLCSWLQRLGCEVVDAGILPDDLSTTRARL
GELQDVDLILSTGGVSVGEADFLGIALREEGELTLWKLAIKPGKPLTVGHFRGVPVIGLPGNPASTLVTFALLTRPYLLR
LQGVQDVEPLKFSVPAGFVWPQAGNRREFLRGRLEQGQAIIYRNQSSGVLRSAAWAEGLVEVREGRVLVKGDQVLFIPLS
ELLG

Specific function: Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP [H]

COG id: COG0303

COG function: function code H; Molybdopterin biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moeA family [H]

Homologues:

Organism=Homo sapiens, GI66932975, Length=335, Percent_Identity=35.2238805970149, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI10880983, Length=335, Percent_Identity=35.2238805970149, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1787049, Length=321, Percent_Identity=49.8442367601246, Blast_Score=278, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI25147659, Length=380, Percent_Identity=31.3157894736842, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17562606, Length=329, Percent_Identity=27.6595744680851, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17136978, Length=347, Percent_Identity=30.5475504322767, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24638841, Length=347, Percent_Identity=30.5475504322767, Blast_Score=142, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020817
- InterPro:   IPR008284
- InterPro:   IPR005111
- InterPro:   IPR005110
- InterPro:   IPR001453 [H]

Pfam domain/function: PF00994 MoCF_biosynth; PF03454 MoeA_C; PF03453 MoeA_N [H]

EC number: NA

Molecular weight: Translated: 43339; Mature: 43207

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00178 AA_TRNA_LIGASE_I ; PS01079 MOCF_BIOSYNTHESIS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKPGKTGSLMPVEAALTRLLEMAEGSRIVESEFLPLAQVQGRVLAQDLVSTLDLPPWPN
CCCCCCCCCCCCHHHHHHHHHHHHCCCEEHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
SAMDGYALNLADWTGEPLPVSQKVFAGQAPEPLKPRTCARIFTGAPVPAGADCVEMQENV
CCCCCEEEEECCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHCCC
EIHADERVSFTETMVSGQNIRPQGQETTVGELVLPAGTRLGPIEQGLAASLGCAGLEVVR
EEECCCCCCHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCHHHHHHHH
RVRVAVLSTGDELVEPGTPLGPGQIYNSNRVLLCSWLQRLGCEVVDAGILPDDLSTTRAR
HHHHHHCCCCHHHHCCCCCCCCCEEECCCCEEHHHHHHHHCCEEEECCCCCCCHHHHHHH
LGELQDVDLILSTGGVSVGEADFLGIALREEGELTLWKLAIKPGKPLTVGHFRGVPVIGL
HCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCEEEECCCCCCEEEC
PGNPASTLVTFALLTRPYLLRLQGVQDVEPLKFSVPAGFVWPQAGNRREFLRGRLEQGQA
CCCCHHHHHHHHHHHCCCEEEECCCCCCCCEEEECCCCEECCCCCCHHHHHHHHHCCCCE
IIYRNQSSGVLRSAAWAEGLVEVREGRVLVKGDQVLFIPLSELLG
EEEECCCCCHHHHHHHHHHHHHHHCCEEEEECCEEEEEEHHHHCC
>Mature Secondary Structure 
GKPGKTGSLMPVEAALTRLLEMAEGSRIVESEFLPLAQVQGRVLAQDLVSTLDLPPWPN
CCCCCCCCCCCHHHHHHHHHHHHCCCEEHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
SAMDGYALNLADWTGEPLPVSQKVFAGQAPEPLKPRTCARIFTGAPVPAGADCVEMQENV
CCCCCEEEEECCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHCCC
EIHADERVSFTETMVSGQNIRPQGQETTVGELVLPAGTRLGPIEQGLAASLGCAGLEVVR
EEECCCCCCHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCHHHHHHHH
RVRVAVLSTGDELVEPGTPLGPGQIYNSNRVLLCSWLQRLGCEVVDAGILPDDLSTTRAR
HHHHHHCCCCHHHHCCCCCCCCCEEECCCCEEHHHHHHHHCCEEEECCCCCCCHHHHHHH
LGELQDVDLILSTGGVSVGEADFLGIALREEGELTLWKLAIKPGKPLTVGHFRGVPVIGL
HCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCEEEECCCCCCEEEC
PGNPASTLVTFALLTRPYLLRLQGVQDVEPLKFSVPAGFVWPQAGNRREFLRGRLEQGQA
CCCCHHHHHHHHHHHCCCEEEECCCCCCCCEEEECCCCEECCCCCCHHHHHHHHHCCCCE
IIYRNQSSGVLRSAAWAEGLVEVREGRVLVKGDQVLFIPLSELLG
EEEECCCCCHHHHHHHHHHHHHHHCCEEEEECCEEEEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]