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The map label for this gene is ynfL [H]

Identifier: 77460119

GI number: 77460119

Start: 4409382

End: 4410317

Strand: Direct

Name: ynfL [H]

Synonym: Pfl01_3898

Alternate gene names: 77460119

Gene position: 4409382-4410317 (Clockwise)

Preceding gene: 77460115

Following gene: 77460123

Centisome position: 68.49

GC content: 62.71

Gene sequence:

>936_bases
ATGGCCGAACGCGACGTCCAGCGCCTGCTCAACGACCGCCTCGACTGGAACCTGTTGCGCACCTTTCGGGTGATCGGTCA
GGAGCTGAGCATCAGCCGCGCCGCCGCGCGTCTGCACCTGACGCAACCGGCGGTGAGCCAGGCGCTCAAGCGTCTTGAGG
AGCAACTGGGTCGCCAGCTGATCGCCCGGCGCGGCCCGCGCTTTGCCCTTACCGAAGTCGGCGAGCAGATCTTCGAACTG
GCCGGCGAGATCTACGGGCAGATGTCCCAGGTCAGCAGCCTGCTGGAGCAACCGGCCGACGAAGTGATCGGCAAGGTGCG
ACTGCTGATCATCAGTCGGATCTTTTCCGATCGCTTCGATGACTTCCTCGCCGACTTCCATAGGCTGTATCCACGGGTCG
ATCTGGAGGTCGACGTGATGCGCAGTTCGGACATCGTCAGCGCCCTTCAGGAGAAAACCGCGACCCTCGGCCTGAGCCTC
AATCGCCGCCCGCAACCGCGTCTGGAACAGCGTCTGTTTCTGCGTCAACGCTATGCGTTTTTCTGTGGCAAACACCACGC
GCTGTTTGGCCGACAGGACATCGGCGAAGGCGATCTGCAGCGTGAGAACTTCGTCAGTTTCACCAGCGACCAGATTGGCG
GCATGCTCTCGCCGCTGACGATTTTTCGCGATCAACAGGGCTTCAGCGGACGCATCGTTGCCTCCTCGCCAAGCCTGGAA
GAGGTGCGGCGACTGGTGATCGCCGGGTTCGGTATCGGTTGTTTGCCGGAGCATGTGGTGGCGGCGGACGTCGAGGCCGG
ATTGCTCTGGCGCCTGCCGCCCCACGAGGGGATTGCCGATGTCGACATTCATCTGCTGTGGAATCGCGAGCAGCGCATGA
GCCGCGCCGAGACGCTGTTCATCGAACGCTTGCAGGCCTGTCTGGCGGATCAGTAA

Upstream 100 bases:

>100_bases
CGGCGCCTGTCGTGGGGTGATTTATCGTCCCGGGGCTATGATTAGTCAATTTTCTATTTATTATTCCGAGTATTACCCTT
CCTTATAATCGAGAAATCCC

Downstream 100 bases:

>100_bases
CCGAGTAGCCGATCCGCGCTGTTGAGCAAAGGCTCACCGGCATTCAGACGGCGGAAGTTCTCGGCGATCTGCTCCGCAAT
GCAGTCATGAGACGCCGCCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MAERDVQRLLNDRLDWNLLRTFRVIGQELSISRAAARLHLTQPAVSQALKRLEEQLGRQLIARRGPRFALTEVGEQIFEL
AGEIYGQMSQVSSLLEQPADEVIGKVRLLIISRIFSDRFDDFLADFHRLYPRVDLEVDVMRSSDIVSALQEKTATLGLSL
NRRPQPRLEQRLFLRQRYAFFCGKHHALFGRQDIGEGDLQRENFVSFTSDQIGGMLSPLTIFRDQQGFSGRIVASSPSLE
EVRRLVIAGFGIGCLPEHVVAADVEAGLLWRLPPHEGIADVDIHLLWNREQRMSRAETLFIERLQACLADQ

Sequences:

>Translated_311_residues
MAERDVQRLLNDRLDWNLLRTFRVIGQELSISRAAARLHLTQPAVSQALKRLEEQLGRQLIARRGPRFALTEVGEQIFEL
AGEIYGQMSQVSSLLEQPADEVIGKVRLLIISRIFSDRFDDFLADFHRLYPRVDLEVDVMRSSDIVSALQEKTATLGLSL
NRRPQPRLEQRLFLRQRYAFFCGKHHALFGRQDIGEGDLQRENFVSFTSDQIGGMLSPLTIFRDQQGFSGRIVASSPSLE
EVRRLVIAGFGIGCLPEHVVAADVEAGLLWRLPPHEGIADVDIHLLWNREQRMSRAETLFIERLQACLADQ
>Mature_310_residues
AERDVQRLLNDRLDWNLLRTFRVIGQELSISRAAARLHLTQPAVSQALKRLEEQLGRQLIARRGPRFALTEVGEQIFELA
GEIYGQMSQVSSLLEQPADEVIGKVRLLIISRIFSDRFDDFLADFHRLYPRVDLEVDVMRSSDIVSALQEKTATLGLSLN
RRPQPRLEQRLFLRQRYAFFCGKHHALFGRQDIGEGDLQRENFVSFTSDQIGGMLSPLTIFRDQQGFSGRIVASSPSLEE
VRRLVIAGFGIGCLPEHVVAADVEAGLLWRLPPHEGIADVDIHLLWNREQRMSRAETLFIERLQACLADQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=283, Percent_Identity=26.1484098939929, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI157672245, Length=228, Percent_Identity=24.5614035087719, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790262, Length=257, Percent_Identity=25.6809338521401, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI2367136, Length=123, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787530, Length=291, Percent_Identity=21.3058419243986, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=281, Percent_Identity=21.7081850533808, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788748, Length=238, Percent_Identity=23.5294117647059, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1786401, Length=256, Percent_Identity=26.171875, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35441; Mature: 35310

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERDVQRLLNDRLDWNLLRTFRVIGQELSISRAAARLHLTQPAVSQALKRLEEQLGRQL
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IARRGPRFALTEVGEQIFELAGEIYGQMSQVSSLLEQPADEVIGKVRLLIISRIFSDRFD
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
DFLADFHRLYPRVDLEVDVMRSSDIVSALQEKTATLGLSLNRRPQPRLEQRLFLRQRYAF
HHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
FCGKHHALFGRQDIGEGDLQRENFVSFTSDQIGGMLSPLTIFRDQQGFSGRIVASSPSLE
HHCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEECCCCHH
EVRRLVIAGFGIGCLPEHVVAADVEAGLLWRLPPHEGIADVDIHLLWNREQRMSRAETLF
HHHHHHHHCCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEECHHHHHHHHHHHH
IERLQACLADQ
HHHHHHHHCCC
>Mature Secondary Structure 
AERDVQRLLNDRLDWNLLRTFRVIGQELSISRAAARLHLTQPAVSQALKRLEEQLGRQL
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IARRGPRFALTEVGEQIFELAGEIYGQMSQVSSLLEQPADEVIGKVRLLIISRIFSDRFD
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
DFLADFHRLYPRVDLEVDVMRSSDIVSALQEKTATLGLSLNRRPQPRLEQRLFLRQRYAF
HHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
FCGKHHALFGRQDIGEGDLQRENFVSFTSDQIGGMLSPLTIFRDQQGFSGRIVASSPSLE
HHCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEECCCCHH
EVRRLVIAGFGIGCLPEHVVAADVEAGLLWRLPPHEGIADVDIHLLWNREQRMSRAETLF
HHHHHHHHCCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEECHHHHHHHHHHHH
IERLQACLADQ
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]